Metrosep Carb 2 Carbohydrate separation column for ion chromatography Carbohydrate analysis with the Metrosep Carb 2 separation column Metrohm is continually expanding its own range of co 02 lumns and improving existing separation column types using state­of­the­art manufacturing processes and materials. The result is columns with excellent separating efficiency, short retention times, high stability, and low costs per injection. The high­capacity Metrosep Carb 2 carbohydrate separa­ The Metrosep carbohydrate separation column is availtion column is available as an iColumn. «i» stands for in able in three lengths (100, 150, and 250 mm), each with tel ligent: In addition to the serial number of the column, inner diameters of 4.0 and 2.0 mm. It is ideally suited to all of its function parameters (e.g., maximum permissible the separation of sugar alcohols, monosaccharides, and pressure and maximum permissible flow) are saved on a disaccharides. single chip. Furthermore, data such as the operating hours and the number of determinations is recorded automatically and is made available to the IC system for monitoring the optimum performance of the column. Column dimensions 100/4.0 150/4.0 250/4.0 1 2 3 4 5 6 7 8 (mm) 1 2.5 mg/L inositol × × × 2 5 mg/L arabitol × × × 1 2 3 4 5 6 7 8 3 5 mg/L sorbitol × × 4 5 mg/L glucose × × × 1 2 4 6 8 5 5 mg/L xylose × × 6 5 mg/L fructose × × × 7 10 mg/L lactose × × 8 15 mg/L sucrose × × × Effect of the column length on the resolution and the analysis times under standard conditions using pulsed amperometric detection: Eluent: 100 mmol/L sodium hydroxide + 10 mmol/L sodium acetate, column temperature 30°C; sample volume 20 µL. Flow for 100 mm column 0.8 mL/min, for 150 and 250 mm columns 0.5 mL/min, amperometric detector: PAD mode, WE: Au, RE: Pd, working potential: 50 mV Applications The Metrosep Carb 2 is ideally suited for routine analysis Typical applications 03 of carbohydrates and their derivatives. It has applications • Sugars, e.g. monosaccharides, oligosaccharides, and across a range of industries: Water and environmental polysaccharides, etc. analysis, in the pharmaceutical and food industries, fo ­ • Sugar alcohols, polyols, glycols, etc. ren sic analysis, quality control of biofuels and cosmetics, • Amino sugars, e.g. aminoglucose, galactosamine, etc. and in life sciences. • Anhydrous sugars, e.g. levoglucosan, galactosan, mannosan, etc. • Saccharic acids, e.g. glucuronic acid, gluconic acid, sialic acid, etc. • Substituted sugar compounds, e.g. glucose­6­phosphate, 2­fluoro­2­deoxy­D­glucose, etc. • Nitrite, bromide, nitrate in seawater with UV/VIS detection 1000 5500 5000 900 4500 800 4000 arabitol 700 sucrose; 65 g/L 3500 sorbitol 600 3000 mannitol inositol 500 2500 erythritol mannosan galactosan Current [nA] 400 2000 levoglucosan Current [nA] glucose; 10 g/L lactose; 31 g/L 1500 rhamnose 300 xylose galactose; 4 g/L 1000 glucose 200 fructose; 15 g/L 500 sucrose 100 0 Systempeak 0 5 10 15 20 25 30 35 40 45 50 55 0 4 8 12 16 20 24 28 32 36 40 Time [min] Time [min] Sugar determination in yogurt using Inline Dialysis Air particle analysis of tracers such as levoglucosan Metrosep Carb 2 ­ 150/2.0 from wood burning sources 5 mmol/L sodium hydroxide / 2 mmol/L sodium acetate, column Metrosep Carb 2 ­ 150/4.0 temperature 40°C; sample volume 5 µL, flow 0.13 mL/min, 10 mmol/L sodium hydroxide, column temperature 45°C; amperometric detector: PAD mode, WE: Au, RE: Pd, working sample volume 100 µL, flow 1.0 mL/min, amperometric detector: potential: 50 mV PAD mode, WE: Au, RE: Pd, working potential: 50 mV 260 240 1600 220 1400 200 180 nitrate; 10 mg/L U] 1200 galactose glucose 160 1000 140 120 800 100 Current [nA] nitrite; 2 mg/L Absorption [mA 80 600 60 bromide; 10 mg/L 400 lactose 40 20 200 0 0 0 1 2 3 4 5 6 7 8 9 10 11 12 0 2 4 6 8 10 12 14 16 18 20 Time [min] Time [min] Seawater analysis after Inline Ultrafiltration Analysis of lactose in lactose-free milk diluted 1:100 Metrosep Carb 2 ­ 100/2.0 after Inline Dialysis 10 g/L NaCl, column temperature 30°C, sample volume 20 µL, Metrosep Carb 2 ­ 150/4.0 flow 0.375 mL/min, UV/VIS detector: 218 ± 5 nm 5 mmol/L sodium hydroxide / 2 mmol/L sodium acetate, column temperature 40°C; sample volume 20 µL, flow 0.8 mL/min, ampe rometric detector: PAD mode, WE: Au, RE: Pd, working potential: 50 mV. The sample was augmented with 100 mg/L lactose. The Metrosep Carb 2 exhibits optimum peak shapes. Free selection of column length allows for a high degree of flexibility, depending on the application. Technical information Housing PEEK Substrate Polystyrene­divinylbenzene copolymer with quaternary ammonium groups Standard eluent 100 mmol/L sodium hydroxide + 10 mmol/L sodium acetate Standard flow Maximum flow Metrosep Carb 2 - 100/4.0 0.8 mL/min 1.6 mL/min Metrosep Carb 2 - 150/4.0 0.5 mL/min 1.2 mL/min Metrosep Carb 2 - 250/4.0 0.5 mL/min 0.8 mL/min Metrosep Carb 2 - 100/2.0 0.2 mL/min 0.7 mL/min Metrosep Carb 2 - 150/2.0 0.13 mL/min 0.45 mL/min Metrosep Carb 2 - 250/2.0 0.13 mL/min 0.30 mL/min Maximum pressure 20 MPa Particle size 5 µm Standard temperature 30°C Temperature range 20–60°C pH range 0–14 Organic modifiers (in the eluent) 0–50% acetonitrile and methanol Organic modifiers (in the sample) 0–100% acetone, acetonitrile, and methanol Storage In standard eluent Can be combined with the Metrosep BO 3– Trap 1 ­ 100/4.0 (6.1015.200) and Metrosep CO 2– Trap 1 ­ 100/4.0 (6.1015.300). 3 3 Ordering information 6.1090.410 Metrosep Carb 2 ­ 100/4.0 6.1090.420 Metrosep Carb 2 ­ 150/4.0 6.1090.430 Metrosep Carb 2 ­ 250/4.0 6.1090.500 Metrosep Carb 2 Guard/4.0 6.1090.510 Metrosep Carb 2 S­Guard/4.0 6.01090.210 Metrosep Carb 2 ­ 100/2.0 6.01090.220 Metrosep Carb 2 ­ 150/2.0 6.01090.230 Metrosep Carb 2 ­ 250/2.0 6.01090.600 Metrosep Carb 2 Guard/2.0 6.01090.610 Metrosep Carb 2 S­Guard/2.0 ­9100 Herisau 6.01806.000 PEEK capillary 0.18 mm ID, 2 m G, CH ohm A witzerland by Metr www.metrohm.com , printed in S SW ­07 Subject to modifications Layout by Ecknauer+Schoch A 8.107.5004EN – 2016