Flyer_MetrosepC4_e Seite 4/1

Metrosep C 4

Cation separation column Application examples

The selectivity for cations of the C 4 column is comparable to that of the C 2 column. This permits the C 4

column to be used in the same application areas as the C 2 column, e.g. for the determination of the standard cations, of amines and transition metals.

2002.0

mine

686

a

minea

Ca

688

2003.5

methyl

dimethyl

690

2005.0

692

minea

Mg

l (μS/cm)

694

2006.5

Na

signal (μS/cm)

signa

trimethyl

verinea

696

d

mine

putrescine

a

ca

2008.0

hist

698

K

700

2009.5

0

4

8

12

16

0

3

6

9

12

15

time (min)

time (min)

Sample: drinking water; column: Metrosep C 4 –

Sample: biogenic amines (5 mg/L methylamine, 100; eluent: 1.7 mmol/L HNO

10 mg/L of each dimethylamine, trimethylamine, 3, 0.7 mmol/L DPA;

temperature: 25 °C, flow: 0.9 mL/min.

putrescine, cadaverine and histamine); Metrosep C 4

column: Metrosep C 4 – 100; eluent: 6 mmol/L

HNO3; temperature: 25 °C, flow: 0.9 mL/min.

Technical information Column material

Maximum pressure

Silica gel with carboxyl groups, particle size 5 μm 6.1050.450:

6.0 MPa (60 bar)

6.1050.410:

12.5 MPa (125 bar)

6.1050.420:

15.0 MPa (150 bar)

New cation separation column Dimensions

6.1050.430:

20.0 MPa (200 bar)

6.1050.450:

50 x 4.0 mm

for ion chromatography 6.1050.410:

100 x 4.0 mm

Maximum flow

6.1050.420:

150 x 4.0 mm

2.0 mL/min (recommended flow: 0.9 mL/min) 6.1050.430:

250 x 4.0 mm

pH range

2...7

Ordering information

ohm Ltd., Herisau, Switzerland 6.1050.450

Cation column Metrosep C 4 – 50

6.1050.430

Cation column Metrosep C 4 – 250

6.1050.410

Cation column Metrosep C 4 – 100

6.1050.500

Precolumn Metrosep C 4 Guard 6.1050.420

Cation column Metrosep C 4 – 150

6.1050.510

Precolumn Metrosep C 4 S-Guard

, printed by Metr

Metrohm Ltd.

CH-9101 Herisau/Switzerland Phone +41 71 353 85 85

Fax +41 71 353 89 01

www.metrohm.com

info@metrohm.com

Subject to modifications Layout by Ecknauer+Schoch ASW

8.107.5001EN – 2008-04





Flyer_MetrosepC4_e Seite 2/3

Metrosep C 4

Cation separation column for ion chromatography Cation separation column New column type

Advantages of the new column Metrohm is continuously expanding its range of Measurements clearly show the advantages of the columns and improving existing separation column new Metrosep C 4 – 150 separation column com-types by applying the latest production methods pared with the Metrosep C 2 – 150 used up to now.

and materials. This results in optimized columns Metrosep C 4 – 50

Especially for monovalent cations, the new Metrosep with exceptional separation performance, short 5 μS/cm

C 4 yields peaks that are not only more symmetrical retention times, high stability and low cost per but also sharper and therefore higher. This improves injection.

resolution and results in lower detection limits. All the analyses carried out show that the separation Metrosep C 4 – 100

The Metrosep C 4 separation column replaces the performance of the Metrosep C 4 is superior to that two column types Metrosep Cation 1-2 and Metro-of the Metrosep C 2.

sep C 2. The C 4 column is available as iColumn, i.e. as an intelligent separation column, in column Metrosep C 4 – 150

lengths of 50 mm, 100 mm, 150 mm and 250 mm.

692

The diagram shows the influence of the column 692

Metrosep C 4 – 250

length on the resolution and the analysis times for 693

693

4

the separation of the standard cations.

HN

694

694

0

10

20

30

40

695

695

4

time (min)

Na

H

Na

N

696

696

Li

K

Li

Mg

Mg

l (μS/cm)

l (μS/cm)

697

Ca

Influence of column length on resolution and 697

Ca

signa

signa

K

analysis times.

698

698

Sample: cation standard (1 mg/L Li+, 5 mg/L Na+, 699

699

5 mg/L NH +

4 , 10 mg/L of each K+, Ca2+ and Mg2+

700

for C 4 – 100, 150 and 250; C 4 – 50 without 700

0

4

8

12

16

20

0

4

8

12

16

20

NH +

4 ); eluent: 1.7 mmol/L HNO3, 0.7 mmol/L DPA; time (min)

time (min)

temperature: 25 °C, flow: 0.9 mL/min.

Separation of the standard cations on the Metrosep C 4 – 150 (left) and Metrosep C 2 – 150 (right).

Modified standard eluent The measurements result in clear differences with respect to peak shape, peak height, resolution and plate count – the Metrosep C 4 demonstrates its superiority.

The standard eluent for the Metrosep C 2 column was an aqueous solution of tartaric acid and dipico-Eluent: 1.7 mmol/L HNO3, 0.7 mmol/L DPA; flow: linic acid. As tartaric acid has been found to pro-0.9 mL/min; sample: cation standard (1 mg/L Li+, mote bacterial growth, it has been replaced with nitric 5 mg/L Na+, 5 mg/L NH +

4 , 10 mg/L of each K+, acid to prolong column life. The new standard eluent Ca2+ and Mg2+).

for the C 4 column is composed of 1.7 mmol/L nitric acid (HNO3) and 0.7 mmol/L dipicolinic acid (DPA).

All other standard parameters have also been optimized for this new eluent. This, together with the lowered standard flow of 0.9 mL/min not only increases TP C 2

TP C 4

Separation of the standard cations with Metrosep column life but also ensures optimal analytical results.

12000

C 4 – 150 and Metrosep C 2 – 150 – comparison 9830

of plate counts. The markedly improved peak 9590

10000

shapes result in higher plate counts, which allows Current Column

Recommended Metrosep C 4 column*

7880

for lower detection limits for the Metrosep C 4.

8000

Metrosep C 2 – 30

Metrosep C 4 – 50

5820

5710

6000

Eluent: 1.7 mmol/L HNO

Metrosep C 2 – 100

Metrosep C 4 – 100

TP

3, 0.7 mmol/L DPA; flow: 4960

4550

4300

0.9 mL/min; sample: cation standard (1 mg/L Li+, Metrosep C 2 – 150

Metrosep C 4 – 150

4080

4000

3270

5 mg/L Na+, 5 mg/L NH +

3090

4 , 10 mg/L of each K+, Metrosep C 2 – 250

Metrosep C 4 – 250

2930

Ca2+ and Mg2+).

Metrosep Cation 1-2

Metrosep C 4 – 100

2000

Metrosep C 2 Guard

Metrosep C 4 Guard

0

Metrosep C 2 S-Guard

Metrosep C 4 S-Guard

Li

Na

NH

K

Ca

Mg

4

* Please note that additional adaptations may be necessary, as required by the specific application.

cations





Flyer_MetrosepC4_e Seite 2/3

Metrosep C 4

Cation separation column for ion chromatography Cation separation column New column type

Advantages of the new column Metrohm is continuously expanding its range of Measurements clearly show the advantages of the columns and improving existing separation column new Metrosep C 4 – 150 separation column com-types by applying the latest production methods pared with the Metrosep C 2 – 150 used up to now.

and materials. This results in optimized columns Metrosep C 4 – 50

Especially for monovalent cations, the new Metrosep with exceptional separation performance, short 5 μS/cm

C 4 yields peaks that are not only more symmetrical retention times, high stability and low cost per but also sharper and therefore higher. This improves injection.

resolution and results in lower detection limits. All the analyses carried out show that the separation Metrosep C 4 – 100

The Metrosep C 4 separation column replaces the performance of the Metrosep C 4 is superior to that two column types Metrosep Cation 1-2 and Metro-of the Metrosep C 2.

sep C 2. The C 4 column is available as iColumn, i.e. as an intelligent separation column, in column Metrosep C 4 – 150

lengths of 50 mm, 100 mm, 150 mm and 250 mm.

692

The diagram shows the influence of the column 692

Metrosep C 4 – 250

length on the resolution and the analysis times for 693

693

4

the separation of the standard cations.

HN

694

694

0

10

20

30

40

695

695

4

time (min)

Na

H

Na

N

696

696

Li

K

Li

Mg

Mg

l (μS/cm)

l (μS/cm)

697

Ca

Influence of column length on resolution and 697

Ca

signa

signa

K

analysis times.

698

698

Sample: cation standard (1 mg/L Li+, 5 mg/L Na+, 699

699

5 mg/L NH +

4 , 10 mg/L of each K+, Ca2+ and Mg2+

700

for C 4 – 100, 150 and 250; C 4 – 50 without 700

0

4

8

12

16

20

0

4

8

12

16

20

NH +

4 ); eluent: 1.7 mmol/L HNO3, 0.7 mmol/L DPA; time (min)

time (min)

temperature: 25 °C, flow: 0.9 mL/min.

Separation of the standard cations on the Metrosep C 4 – 150 (left) and Metrosep C 2 – 150 (right).

Modified standard eluent The measurements result in clear differences with respect to peak shape, peak height, resolution and plate count – the Metrosep C 4 demonstrates its superiority.

The standard eluent for the Metrosep C 2 column was an aqueous solution of tartaric acid and dipico-Eluent: 1.7 mmol/L HNO3, 0.7 mmol/L DPA; flow: linic acid. As tartaric acid has been found to pro-0.9 mL/min; sample: cation standard (1 mg/L Li+, mote bacterial growth, it has been replaced with nitric 5 mg/L Na+, 5 mg/L NH +

4 , 10 mg/L of each K+, acid to prolong column life. The new standard eluent Ca2+ and Mg2+).

for the C 4 column is composed of 1.7 mmol/L nitric acid (HNO3) and 0.7 mmol/L dipicolinic acid (DPA).

All other standard parameters have also been optimized for this new eluent. This, together with the lowered standard flow of 0.9 mL/min not only increases TP C 2

TP C 4

Separation of the standard cations with Metrosep column life but also ensures optimal analytical results.

12000

C 4 – 150 and Metrosep C 2 – 150 – comparison 9830

of plate counts. The markedly improved peak 9590

10000

shapes result in higher plate counts, which allows Current Column

Recommended Metrosep C 4 column*

7880

for lower detection limits for the Metrosep C 4.

8000

Metrosep C 2 – 30

Metrosep C 4 – 50

5820

5710

6000

Eluent: 1.7 mmol/L HNO

Metrosep C 2 – 100

Metrosep C 4 – 100

TP

3, 0.7 mmol/L DPA; flow: 4960

4550

4300

0.9 mL/min; sample: cation standard (1 mg/L Li+, Metrosep C 2 – 150

Metrosep C 4 – 150

4080

4000

3270

5 mg/L Na+, 5 mg/L NH +

3090

4 , 10 mg/L of each K+, Metrosep C 2 – 250

Metrosep C 4 – 250

2930

Ca2+ and Mg2+).

Metrosep Cation 1-2

Metrosep C 4 – 100

2000

Metrosep C 2 Guard

Metrosep C 4 Guard

0

Metrosep C 2 S-Guard

Metrosep C 4 S-Guard

Li

Na

NH

K

Ca

Mg

4

* Please note that additional adaptations may be necessary, as required by the specific application.

cations





Flyer_MetrosepC4_e Seite 4/1

Metrosep C 4

Cation separation column Application examples

The selectivity for cations of the C 4 column is comparable to that of the C 2 column. This permits the C 4

column to be used in the same application areas as the C 2 column, e.g. for the determination of the standard cations, of amines and transition metals.

2002.0

mine

686

a

minea

Ca

688

2003.5

methyl

dimethyl

690

2005.0

692

minea

Mg

l (μS/cm)

694

2006.5

Na

signal (μS/cm)

signa

trimethyl

verinea

696

d

mine

putrescine

a

ca

2008.0

hist

698

K

700

2009.5

0

4

8

12

16

0

3

6

9

12

15

time (min)

time (min)

Sample: drinking water; column: Metrosep C 4 –

Sample: biogenic amines (5 mg/L methylamine, 100; eluent: 1.7 mmol/L HNO

10 mg/L of each dimethylamine, trimethylamine, 3, 0.7 mmol/L DPA;

temperature: 25 °C, flow: 0.9 mL/min.

putrescine, cadaverine and histamine); Metrosep C 4

column: Metrosep C 4 – 100; eluent: 6 mmol/L

HNO3; temperature: 25 °C, flow: 0.9 mL/min.

Technical information Column material

Maximum pressure

Silica gel with carboxyl groups, particle size 5 μm 6.1050.450:

6.0 MPa (60 bar)

6.1050.410:

12.5 MPa (125 bar)

6.1050.420:

15.0 MPa (150 bar)

New cation separation column Dimensions

6.1050.430:

20.0 MPa (200 bar)

6.1050.450:

50 x 4.0 mm

for ion chromatography 6.1050.410:

100 x 4.0 mm

Maximum flow

6.1050.420:

150 x 4.0 mm

2.0 mL/min (recommended flow: 0.9 mL/min) 6.1050.430:

250 x 4.0 mm

pH range

2...7

Ordering information

ohm Ltd., Herisau, Switzerland 6.1050.450

Cation column Metrosep C 4 – 50

6.1050.430

Cation column Metrosep C 4 – 250

6.1050.410

Cation column Metrosep C 4 – 100

6.1050.500

Precolumn Metrosep C 4 Guard 6.1050.420

Cation column Metrosep C 4 – 150

6.1050.510

Precolumn Metrosep C 4 S-Guard

, printed by Metr

Metrohm Ltd.

CH-9101 Herisau/Switzerland Phone +41 71 353 85 85

Fax +41 71 353 89 01

www.metrohm.com

info@metrohm.com

Subject to modifications Layout by Ecknauer+Schoch ASW

8.107.5001EN – 2008-04