bbe biological •biophysical •engineering moldaenke F l u o r o P r o b e Submersible Spectrofluorometer with Automatic Algae Class and Chlorophyll Analysis Gelbstoff measurement (CDOM) by irradiation with UV-LED w w w . b b e - m o l d a e n k e . d e F l u o r o P r o b e b b e m o l d a e n k e © F l u o r o P r o b e – a h i g h l y s e n s i t i v e m e a s u r i n g i n s t r u m e n t Get to know your water ... The bbe FluoroProbe is a highly sensitive measuring instrument for chlorophyll analysis. Due to the numerous features it can be used in a variety of applications. The FluoroProbe quickly and reliably determines chlorophyll concentration down to depths of 100m. The measuring data of the probe can be displayed online via a serial interface or stored in the probe for subsequent analysis. In addition to determining the chlorophyll content, the probe also detects the presence of algae and allocates them to various spectral algae classes (blue-green algae/cyanobacteria, green algae, diatoms/dinoflagellates/chrysophytae, cryptophytae). This enables the instrument to analyse the occurrence and distribution of algae on site without the necessity of the laboratory. Minimum set-up – maximum precision D no sample preparation D 'click and go' D autostart with plug-in D calculation in real time D instant graphic plot function D profile and/or time response display D pre-adjustment of algae classes D measurements according to HPLC-pigment analysis D best fit for composite algae mixtures F l u o r o P r o b e b b e m o l d a e n k e © In situ chlorophyll profiling plus real-time algae class distinction The software of the FluoroProbe provides detailed data analysis The results can be shown either as tables or graphically. Further processing allows zooming, printing and export of data. Also setting the parameter and calibrating the FluoroProbe is performed with the help of the PC software. The screenshots show an example of distribution profiles recorded with the FluoroProbe in Lake Plussee (Northern Germany). The vertical migration of dinoflagellates was measured, both measurements were taken at the same location at 10:00 am (upper graph) and 12:00 noon (lower graph). Dinoflagellates were dominant. The fluorescence of algae due to excitation by visible light mainly depends on the presence of chlorophyll-a, a very common pigment in the plant world. The occurrence of other pigments is typical for different algae classes. Interactions between these different pigment systems with chlorophyll-a results in a special excitation spectrum for the taxoni-mical algae classes. The special patterns of 1 0 : 0 0 this algae fluorescence - so-called fingerprints - are used in the bbe fluorometers for the quantification of different algae Most of the dinoflagellates were situated at the surface classes. The excitation light sources are (0-2 m) of the lake. A maximum of cryptophyta was found at LEDs with different wavelengths, mostly circa 5 m. within the visible spectral range. The fingerprints of four algae classes and of Gelbstoff are already stored in the FluoroProbe. Special user-defi- ned finger-prints can also be added and used to analyse the chlorophyl con- tent of the water. P D A f o r O p e r a t i o n i n t h e f i e l d 1 2 : 0 0 Gelbstoff measurement – CDOM Decay of organic matter often results in The dinoflagellates had moved downwards in water layers with products with fluorescent properties. higher nutrient concentrations. Their maximum concentration was found at 3 m depth, whilst the other algal groups did not Excitation with UV-light records Gelb-migrate. The peak of cryptophyta remained stable at 5 m depth stoff and allows blank readings/zero throughout the entire day. point correction for most cases with internally stored Gelbstoff fingerprints. w w w . b b e - m o l d a e n k e . d e F l u o r o P r o b e b b e m o l d a e n k e © Applications D drinking water monitoring for blue-green algae D supervision of aquaculture systems D control of bathing water for toxic algae blooms D early warning system D ocean research D limnological research W o r k s t a t i o n 2 5 D education Specifications F l u o r o P r o b e Measurands total chlorophyll [µg chl-a/l] Chlorophyll 0 - 200 µg chl-a/l Measurement procedure spectral fluorometry Resolution 0.05 µg chl-a/l Features Transmission 0 - 100% D determination of up to 5 different algae classes Water temperature -2 - +40°C D quantification of different Housing material reinforced carbon fibre/V4A steel algae classes within seconds Weight 4.5 kg by in situ measurement D Gelbstoff measurement Dimensions (H x D) 450 x 140 mm with UV-LED Voltage 12 V D reduction of laborious microscopic observations – saving valuable Battery capacitiy 3900 mAh time Operating time 10 h - 30 days D internal rechargable battery for stand-alone measurements Memory capacity 1800 datasets D internal datalogger Interface RS485/RS232 modem (optional) D pressure sensor Maximum diving depth 0 - 100 m 0 - 300 m (extended range 1) D PC software for data analysis 0 - 1000 m (extended range 2) included D optional water temperature Options Workstation 25 (cuvette device) sensor transmission measurement ation! temperature measurement er D optional transmission sensor instrument for benthic algae o alt PDA with software D optional GSM modem 2, 10, 20, 50, 100 m cables Subject t Your local bbe dealer… biological • biophysical • engineering bbe moldaenke Wildrosenweg 3 | 24119 Kronshagen | Germany phone (+49) 431 38040 -0 fax (+49) 431 38040 -10 e-mail info@bbe-moldaenke.de net www.bbe-moldaenke.de