AZURA Bio LC 2 FPLC and Purification Solutions Flexible and adjustable to your biopurifi cation tasks Fast protein liquid chromatography (FPLC) of appropriate pump from KNAUER. A wide range of bio molecules can be a task. KNAUER offers with fl ow cells for different detectors allows optimized AZURA® an elaborate biochromatography concept, detection. The unique benchtop cooling feature which builds on our experience and is makes costly cooling compartments unnecessary very adaptable. AZURA Bio LC is designed for and enables operation in any lab environment. performance, fl exibility and user friendliness. KNAUER supports you in system confi guration and System solutions range from fl ow rates as low as application development. 0.02 ml/min up to 1000 ml/min by choosing the Oligonucleotides Antibodies Other proteins Peptides Biomolecules Enzymes Hormones Vaccines Biosimilars/ Biopharmaceuticals 2 How much product do you want to purify? Contichrom MCSGP Up to 100 ml/min 200 g/day Twin column process www.knauer.net/contichrom Purity AZURA SMB w Productivity b ww Flexibility Up to . 500 ml/min kn AZURA Bio LC a 2000 g/day uer. Up to ne Multi column process t 1000 ml/min /a SMB Principle zu www.knauer.net/sm Feed Raf R finate inat 5 – 10 g/day rabio Single column process Column Switching Extract Eluent AZURA® Bio LC features 2 Isocratic, binary high pressure or quaternary 2 A variety of detectors (UV/VIS, DAD, RI) low pressure gradient pumps and monitors (conductivity and pH) 2 Pump heads allowing fl ow rates of 2 Wide range of fl ow cells available, up to 10, 50, 100, 250, 500 or 1000 ml/min including remote cells with fi ber optics 2 Bio-inert wetted materials 2 Integrated leak management and sensors 2 Optional benchtop cooling as well as software, monitoring the solvent of glass column and fraction collector supply and waste, provide for safety 2 Choice of different control software packages: volume-based or time-based 3 AZURA Bio LC Purifi cation Solutions Designing tailor-made FPLC purifi cation solutions is very easy with the modular AZURA Bio LC platform. Scalable regarding the purifi ed amount and automatable ranging from basic to sophisticated, you have the choice. Here are a few examples. AZURA Compact Bio LC Flow rate: Max. 10 ml/min or 50 ml/min 1/16‘‘ Auto- Variable Available Low High Automated sampler Automated Variable multiple Automated AZURA max. fl ow rates pressure pressure Manual sample with buffer Column Peak single wavelength DAD Conductivity fraction System features ml/min gradient gradient sample injection cooling selection switching parking Air sensor wavelength detection detection and collection and options 10 50 100 250 500 1000 option option injection module option module module module option detection option option pH option option AZURA Compact Bio LC            AZURA Lab Bio LC LPG               AZURA Lab Bio LC HPG                AZURA Pilot Bio LC                 4 Maximum Flowrate AZURA Lab Bio LC Flow rate: Max. 10 ml/min or 50 ml/min 1/16‘‘ AZURA Pilot Bio LC Flow rate: Max. 100, 250, 500, or 1000 ml/min 1/8‘‘ or 1/4‘‘ Auto- Variable Available Low High Automated sampler Automated Variable multiple Automated AZURA max. fl ow rates pressure pressure Manual sample with buffer Column Peak single wavelength DAD Conductivity fraction System features ml/min gradient gradient sample injection cooling selection switching parking Air sensor wavelength detection detection and collection and options 10 50 100 250 500 1000 option option injection module option module module module option detection option option pH option option AZURA Compact Bio LC            AZURA Lab Bio LC LPG               AZURA Lab Bio LC HPG                AZURA Pilot Bio LC                 www.knauer.net/azurabio 5 What is your idea of straightforward FPLC? The AZURA Compact Bio LC systems were designed for routine separation tasks in research and develop ment. Thanks to the compact design and easy to use PurityChrom® Bio software, the systems offer reliable performance and ease-of-use. The AZURA Compact Bio LC systems are resistant to all commonly used buffer solutions since all wetted materials are composed of biocompatible parts. The systems can also be operated with organic solvents typically used in HPLC. 2 AZURA Compact Bio LC Isocratic buffer supply • Pump with a fl ow rate of up to 10 ml/min or 50 ml/min • UV detector with variable wavelength (190 – 500 nm) • Manual injection valve 6 www.knauer.net/compactbio AZURA Compact Bio LC Application Separation of a protein standard by size exclusion chromatography (SEC) Manual injection valve Material and column dimension BioFox 17/1200 SEC, Pre-packed column 300 x 8 mm ID , 15 ml Buffer: 20 mM sodium phosphate buffer pH 7.0, 300 mM NaCl Gradient: isocratic Flow rate: 0.125 ml/min Temperature: ambient Injection volume: 10 µl Sample: size exclusion chromatography standard (BioRad 151-190) 60 6 50 5 40 30 4 AU 20 3 m mAU 2 10 1 0 -10 Variable wavelength -20 20 40 60 80 100 120 140 UV/VIS detector Minutes Minutes \\PC-SPA-BIOLAB1\OL_Projects\AP_Bio_05_2013\Result\151013 BioRad 1200 300mM Nacl 0125mlBioLine_3.rslt\151013 BioRad 1200 300mM Nacl 0125ml.dat, S 2600 [Channel 1] 1 Aggregate 2 Thyroglobulin 670 kDa 3 gamma-Globulin 158 kDa 4 Ovalbumin 44 kD 5 Myoglobulin 17 kDa 6 Vitamin B12 1.35 kDa www.knauer.net/applications 7 How customizable is your LC purifi cation system? 2 AZURA Lab Bio LC AZURA Lab Bio LC is available as isocratic, low pressure gradient (LPG), or high pressure gradient (HPG) system for the purifi cation of biomolecules on a labora-tory scale. All modules can be freely combined so that for any task the desired device confi guration can be compiled. With AZURA Lab Bio LC you can choose your method: 2 Affi nity chromatography 2 Size exclusion chromatography 2 Ion exchange chromatography 2 Hydrophobic interaction chromatography 2 Mixed mode chromatography The AZURA Bio LC pump can be combined with different assistant modules for sample injection, column switching, peak parking and additional buffer and sample selection as well as fractionation. Detectors, monitors and a wide fl ow cell range enable you to perform accurate detection accompanied by pH and conductivity monitoring regardless of your fl ow rate. An optional fraction collector is available with or without benchtop cooling. Quaternary LPG Pump P 6.1L with online degasser and buffer blending valve (… page 10) 8 www.knauer.net/lab-bio AZURA Lab Bio LC Application Automated purifi cation of human antibodies (IgG) by affi nity chromatography (AC) and successive size exclusion chromatography (SEC) Eluent Tray with tubing guides Assistant ASM 2.1L Material and column dimension for switching tasks Hi Trap Protein G, Protein G Sepharose High Performance, 34 µm, 5 ml BioFox 17/1200 SEC, 300 x 8 mm ID, 15 ml Buffer: A 20 mM Sodium phosphate buffer pH 7.5 Assistant ASM 2.1L with B 100 mM Glycin HCL, pH 2.7 sample pump and valves for C 20 mM Sodium phosphate buffer pH 7.0 injection and switching Method: Gradient Flow rate (… page 14) Affi nity chromatography 0 – 26 min 100 % A 0.5 ml/min Injection and Washing Gel fi ltration 26 – 46 min 100 % B 2 ml/min Elution and Peak- Parking 46 – 86 min 100 % C 1 ml/min UV/VIS Detector UVD 2.1L Re-injection with variable wavelength Temperature: ambient (… page 12) Injection volume: 500 µl Sample: human serum linear Gradient 1 ml/min Tailor your system to 2400 1 AC SEC 2200 make your work easier 2000 1800 2 AZURA Assistant devices may be 1600 confi gured for column switching, 1400 peak parking, fraction collection, 1200 mAUmAU or as a cost effective isocratic FPLC 1000 module: AZURA Compact Bio LC 800 3 2 Choose a specifi c detector 600 2 depending on your target molecule 400 (UV, conductivity/pH, fl uorescence) 200 2 Choice of AZURA pumps depending 0 on your method and desired fl ow rate 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 Minut Minuteses 2 Optional benchtop cooling of \\KNET-OL-LIZ4\Enterprise\Projects\AP_BIO_14_2014\Result\BIOLINE_3.91 2014-07-29 14-23-30 (GMT +02-00).rslt\290714500ulserumshortrunpH2.dat, UVD2.1L [280 nm] glass column and fraction collector 1 Injection peak affi nity chromatography 2 Combinable with all common FPLC 2 Elution peak human IgG affi nity chromatography columns 3 Elution peak human IgG gel fi ltration www.knauer.net/applications 9 Choose the gradient and fl ow rate capabilities you need Pressure Sensor with Buffer Selection Valve Inline Filter two channels for higher fl exibility system protection AZURA Mixer highest mixing 2-Channel effi ciency Online Degasser reduces baseline noise 2 AZURA Pump P 6.1L Binary FPLC Pump (high pressure gradient) • Flow rate range: 0.001 – 50 ml/min • Automatic recognition of pump heads via RFID • Up to 400 bar • Biocompatible, metal-free wetted parts • Pump heads: 10 or 50 ml/min • Binary gradient with solvent selection valve (2 x 2 buffers) www.knauer.net/biopumps 10 2 AZURA Pump P 6.1L Quaternary FPLC Pump (low pressure gradient) • Flow rate range: 0.001 – 10 ml/min • Up to 400 bar • Biocompatible, metal-free wetted parts Multi-Proportioning Valve • Quaternary gradients (4 x 1 buffers) precise blending of buffers Pressure Sensor with Inline Filter system protection AZURA Mixer highest mixing effi ciency 4-Channel Online Degasser reduces baseline noise 2 AZURA Pump P 2.1L Isocratic FPLC Pump • Flow rate range: 0.1 – 1000 ml/min • Pump heads with titanium wetted parts • Gradient options: binary high pressure blending (2 x 1 buffers) with an additional P 2.1L or cost-effective binary low pressure blending (2 x 1 buffers) or ternary low pressure blending (3 x 1 buffers, up to 220 ml/min) Exchangeable Pump Heads 11 Which detector do you need for your target molecules? 2 AZURA Detector DAD 6.1L Diode Array Detector • User friendly and easy maintenance • LightGuide technology • Extended wavelength range 190 – 1000 nm • Impressive sensitivity/price ratio • Fiber optics version available “Versatility through a wide flow cell range“ 2 BlueShadow Detector 50D Multiple Wavelength Detector • Simultaneous measurement of up to 4 wavelengths • Wavelength range from 190 – 750 nm (extended wavelength range version 190 – 900 nm also available) “Robust multi-channel UV/VIS detector” • Wide linear range 2 AZURA Detector UVD 2.1L Variable Wavelength Detector • Wavelength range from 190 – 750 nm • Large choice of flow cells • Fiber optics version available “ Reliable UV/VIS detector for a wide spectrum of applications“ www.knauer.net/biodetect 12 2 AZURA Detector UVD 2.1S Small Variable Wavelength Detector • Very small and compact design • Price attractive • Can be used in an AZURA Assistant • Wavelength range from 190 – 500 nm • Fiber optics version available “Compact and versatile UV detector “ Flow cells for UV/VIS and DAD detectors 2 Flow cells are available for analytical, 2 Fiber optic versions offer the possibility to separate semi-preparative and preparative applications the fl ow cell spatially from the device providing 2 Exchanging fl ow cells is easy due to frontal access enhanced security for hazardous, explosive or toxic work processes Type Capillary Path Volume Max. Max. Wetted connection length fl ow rate pressure materials Analytical 1/16” 10 mm 2 µl 5 ml/min 100 bar PEEK, synthetic Available only quartz glass, Tefl on for DAD 6.1L High sensitivity Available only analytical 1/16” 50 mm 6 µl 5 ml/min 100 bar PEEK, synthetic quartz glass, Tefl on for DAD 6.1L Semi- Fiber optics version preparative 1/16” 3 mm 2 µl 50 ml/min 30 bar PEEK, synthetic quartz glass, Tefl on also available Preparative 1/16” 0.5 mm 3 µl 250 ml/min 100 bar PEEK, synthetic Fiber optics version quartz glass, Tefl on also available Preparative 1/8” Variable Variable 1000 ml/min 100 bar PEEK, synthetic Fiber optics version quartz glass, Tefl on also available Preparative 3/8” with TRI-Clamp connection 7 mm 10 bar PTFE, fused silica Available only as fi ber optics version Preparative 3/8” with TRI-Clamp connection 10 mm 10 bar PTFE, fused silica Available only as fi ber optics version 2 AZURA CM 2.1S Conductivity Monitor • For the monitoring of conductivity and pH • Flow rates up to 100 ml/min • 1 µS/cm – 999 mS/cm • pH range 2 – 12 “ Basic and versatile monitor for Flow cells for Capillary Max. Max. CM 2.1S connection fl ow rate pressure biopurification applications” Analytical 1/16” 10 ml/min 160 bar Preparative 1/16” 100 ml/min 100 bar 13 How often have you wished for an assistant? 2 AZURA ASM 2.1L Injection Module Assistant • Sample pump and automatic injection valve included • Injection via a syringe and sample loop or directly via the sample pump • Injection of different sample amounts from small to large • Sample pump up to 50 ml/min • Pump also usable for column fi lling Equipped with combinations of • Optional: 6 port valve for buffer-valves, pump(s), or detector or sample selection or fraction collection 2 AZURA ASM 2.1L 2 AZURA ASM 2.1L Column Switching Assistant Peak Parking Assistant • Two column switching valves included • Two automatic injection valves included • For up to 6 different columns or up to • Makes peak parking possible 5 different columns and bypass • Enables back fl ushing of columns • Optional: 6 port valve for buffer- or sample • Optional: 6 port valve for buffer selection or selection or fraction collection fraction collection www.knauer.net/asmbio Collecting your products Depending on the number of fractions and volumes to be collected, you can pick either a fraction collector or a fractionation valve to save your precious products. Fractionation Valves 2 For collecting large quantities 2 Up to 5 fractions and waste 2 Available as a single device or integrated into an ASM 2.1L Fraction Collectors available 2 For up to 288 fractions 2 Choice of rack capacities from two 96-well microplates up to nine 480 ml bottles 2 Cooling optional www.knauer.net/fractionation 14 Do you like to measure based on volume or time? 2 PurityChrom® Bio User Friendly Bio LC Software Other supported chromatography data systems with purifi cation function: • Flexible Method development based on volume, • ClarityChrom, a cost-effective and time-based column volume or time CDS • Safety features with “hold function” during • OpenLAB EZChrom Edition, a time-based client/ running method, display of solvent supply and server CDS for large networked laboratories waste management • Approved: 21 CFR part 11 www.knauer.net/softwarebio Your FPLC at your fi nger tips! AZURA Mobile Control enables the control of AZURA Bio liquid chromatography devices. AZURA Mobile Control displays all important parameters of AZURA devices on one screen and works conjointly with the chromato graphy data system OpenLAB and ClarityChrom. The integrated user management ensures that parameters can only be changed by authorized operators. Device settings can be easily changed with sliders or by entering numeric values, for each device and your complete AZURA LC system. A graphical overview of method parameters Download your demo version here: makes the programming very convenient. www.knauer.net/mobilecontrol 15 Pre-packed glass columns for BioFox separation media SEC and IEC Separation media for AC, IEC, IMAC or SEC with a Ready-to-use glass pressure stability up to columns for FPLC are 40 bar (580 psi) for fast available with different and high resolution BioFox agarose media. biochromatography. go to pre-packed FPLC go to BioFox separation columns media www.knauer.net/packedglass www.knauer.net/biofox Bioline glass columns Column rack Bioline glass columns for Designed specifi cally for protein chromatography Bioline high resolution offer axial compression, glass columns, the high pressure stability, Bioline column rack and a thermostatic jacket can also accomodate for easy cooling and any conventional LC or heating. MPLC columns. go to high-resolution go to Bioline column rack glass columns www.knauer.net/glasscolumns www.knauer.net/fplc-rack Application support Benefi t from our long Distributed by: experience in analytical and preparative chromatography and contact us for application support or method development www.knauer.net/appsupp Technical data are subject to change without notice. www.knauer.net HPLC · SMB · Osmometry KNAUER Phone: +49 30 809727-0 Wissenschaftliche Geräte GmbH Telefax: +49 30 8015010 Hegauer Weg 38 E-Mail: info@knauer.net 14163 Berlin, Germany Internet: www.knauer.net © KNAUER 2015 V7855US/2.0/01.15/4t