One instrument for

all applications

2 MSQ Plus™ – mass spectrometer for HPLC/UHPLC analysis





Robust solution

Rugged, easy to use,

high-performance

Nowadays instrumental analysis in quality Resolve routine tasks in contro

ol and in research & development a more effi cient way

means unsupervised sample management The MSQ Plus mass detector handles tasks that often require a lot of time in daily practice much as well as fast and reliable results.

easier due

e to fast and unique mass identifi cation.

With this device, quantitative analysis is more reliable and up to 1000 times more sensitive than UV detection. This means in everyday laboratory life: Using the space-saving, fast and powerful 2 Improvvement of the limit of detection and limit of MSQ P

Plus™ single quadrupole detector quantifi

fication due to increased sensitivity and enhanccement of the calibration range in contrast to together with the high-performance conven

ntional detection procedures..

PLATINblue UHPLC system, maximum 2 Faster m

method development compared with UV

detectio

on as unique results are generated.

productivity without compromises in 2 More reliable peak identifi cation even from trace terms of data quality, sensitivity and impurities or co-eluting peaks.

sturdiness can be achieved.

2 Fast multi-component analysis and confi rmation of molecu

ular weights even when HPLC separation is insuffi cient.

e t.

The Xcalibur™ software easily and 2 Simultaneous analysis of anions and cations possible in one rrun.

comprrehensively controls all LC and MS operating parameters, checks data and compiles result reports. As a matter of course, the software complies with 21 CFR Part 11.

2





Why I use the MSQ?

This MS detector interacts optimally with the PLATINblue HPLC/UHPLC system and facilitates considerably more determinations than systems with classic detectors. The system can be used for micro LC, HPLC and UHPLC. This MS detector supports fl ow rates of up to 2 ml/min without splitting.

Automatic cleaning of

The triple „orthogonal“

The fast change in polarity function facilitates the sample inlet.

M-Path™ design reduces

determination of anions and cations in one run.

neutral background for hig gh

neutral background for hig sensitivity.

The ability to combine the scan and determination of individual ions facilitates identifi cation and quantifi cation in one run.

I can also obtain structural data as a result of the switchable collision-induced dissociation (CID) in the ion source.

The sample inlet is made of titanium making the MSQ Plus extremely robust and resistant to chemicals.

Automatic cleaning of the sample inlet reduces maintenance work and thus downtimes.

I am impressed with the simple operation and high performance. No tools or cables are needed to change the FastLoc ion sources.

The Tefl on covered ion sources can be easily and quickly serviced without tools.

Using the Xcalibur™ data system package, the user can manage the PLATINblue system and the MSQ Plus – control, evaluation and report functions.

The RF prefi lter focuses the ion beam and ensures high

transmission.

Quadrupole analyser with

prefi lter and in-situ

temperature compensation

for high resolution and the highest level of mass stability even during long-term

operation.

Vacuum system with high performance and low power consumption: An integrated effi cient turbo pump generates a vacuum in various regions of the system.

3



It just keeps runn

Fast and simple quantification easy handling

The MSQ Plus mass detector supplies precise mass information for target analytes without the need for expert knowledge.

Groundbreaking features are the ESI and APCI FastLoc ion sources that allow the operating mode to be changed easily and quickly. The ion optic with patented cleaning of the sample inlet provides ultra-robust performance. Simple method development and sequence preparation is supported by templates. An automatic system autotune function and the calibration of mass range do not require the user to make any entries.

Versatile

Using the MSQ Plus mass detector, no compromises have to be made with regard to chromatography. The performance also remains robust when phases with ion pair reagents and non-volatile buffers are used or complex sample matrices are analysed. This mass detector’s extremely wide range of applications covers standard HPLC through to UHPLC.

Ultra-fast scans

Scan speeds of 12000 amu/sec allow for fast data acquisition in shortest time for narrow peaks. In this way, the high throughput of the UHPLC can be used.

Unequalled flow rate range From 10 μl/min to 2 ml/min in electrospray mode; from 200 μl/min to 2 ml/min in APCI mode.

4





ning!

Easy to exchange

The FastLoc ion sources, the ion optic with automated cleaning function and the silently running vacuum system have been optimized to combine high speed with high sensitivity for an extremely high level of robustness.

Ionisation methods

Analytes

FastLoc probes for quickly changing between ESI and ESI:

Preferred ionisation procedure for the APCI mode.

Electro ionisation –

analysis of biomolecules and smaller gentle generation of ions under polar molecules. Typically, quasi-molecu-atmospheric pressure

le ions are detected ([M+H]+ for positive voltage; [M-H]− for negative voltage).

Suitable for molecules up to 20000 m/z.

APCI:

Depending on the solvent and analyte, Chemical ionisation

reactions such as protonation, charge at atmospheric pressure exchange, deprotonation and electron capture are possible.

The method is not as careful as ESI; more fragment ions arise. Suitable for analytes with a molecular weight of up to 2000 m/z. For analytes that are poorly ionised using ESI, that only FastLoc ESI ion source

FastLoc APCI ion source include a few reactive groups and that are thermally stable and vaporable.

HPLC flow rates of capillary For all conventional HPLC

LC to conventional analytical columns with flow rates of 0.2

LC (flow rates of 10 μl/min to to 2 ml/min without splitter 2 ml/min) without splitter Comparison of different single quadrupole mass spectrometers KNAUER

Single Quad Single Quad Single Quad MSQ Plus

vendor 1

vendor 2

vendor 3

Ionisation techniques

Field-tested

ESI sensitivity

50:1

50:1

100:1

50:1

The patented cleaning of the sample inlet maintains the performance of the mass detector even under ESI maximum flow rate

the toughest conditions and reduces the amount of 2

1

1

1

(ml/min)

time-consuming and complicated cleaning steps.

This is a procedure that is normally the case for LC/MS

APCI ion source

included

optional

optional

optional

applications. The figure clearly shows that the critical part of the sample inlet is still clean even after 31 days of heavy-duty use with a phosphate buffer.

Specifications

Mass range (m/z)

2 000

2 000

1 350

2 000

Max. scan speed

12 000

10 000

10 000

15 000

(amu/s)

Width (cm)

30

35

40

35

Indicators

Autotune software





Self-cleaning sample

inlet, maintenance



without tools

5





The software

Intuitive and comprehensive Using practical templates, even beginners can start immediately and develop methods and sequences.

The automatic calibration of the mass range and the autotune software function do not require any user entries.

2 64 scans are possible for each method including the following options:

2 Full scan and selected ion monitoring (SIM) 2 Optional adjustable collision-induced dissociation (CID) in the ion source provides structural data Quan browser: Here the results of the quantification can be reviewed, the parameters can be adjusted, new calibration data can 2

be generated and analyzed samples can be quantified.

Faster change of polarity for positive and negative ESI spectra from scan to scan 2 With a single injection, scan and SIM data can be collected that enable the identification, verification and quantification of the target substances.

2 The software complies with the requirements of 21

CFR Part 11, i.e. system security, multi-user options, implemented audit trails, electronic signatures and much more are included.

Library: The libraries module facilitates the creation of separate MS

databases, the import of MS spectrum data from the Qual browser, the search for unknown substances, and the printing of reports.

In the method editor, the MS methods are created, i.e. scan mode (full scan or SIM) is defined with the respective operational parameters and the ionisation (ESI or APCI) as well as temperature are set.

6





Qual browser: Here the spectra are evaluated, chromatographic peaks in the spectrum are optimized, ion masses and formulas are calculated, the spectra of unknown components are transferred to the library search, and spectra are exported to libraries.

7





The result

It can be this easy

Simplifi ed ion analysis The analysis of ions is greatly simplifi ed using MSQ Plus. By quickly changing the polarity of the ESI mode, you can determine anions and cations in one run.

Separation conditions

Analyte

Anions and cations

(see chromatogram)

Flow rate 0.3

ml/min

Mobile phase

Gradient. H2O/MeOH, 1 % HCOOH

Injection volume

5 μl

Detector (MSQ)

ESI positive/negative mode Capillary voltage 3.5 kV

Temperature

Ion source 350 °C

Identifi cation of unknown compounds The MSQ facilitates unique identifi cation of impurities of an active substance when mAU

Degradation product

compared with conventional UV detection.

A library search of the mass peak for m/z 431

431m/z

fi nds a degradation product of Mevastatin.

Mevastatin

n

Opening the lactone ring forms the corresponding hydroxy acid.

ity

Intens

Separation conditions

Statin D

ra

egr dation:

Analyte

Mevastatin 100 mg/l

Lactone -> Hyd

y roxy acid

Flow rate 1.5

ml/min

Mobile phase

isocratic

H2O/ACN 35:65, 0.1 % HCOOH

Injection volume 10

μl

Detector (MSQ)

ESI positive

Capillary voltage 3.0 kV

Temperature

Ion source 550 °C

m/z

Detection of analytes that do not absorb UV

The analysis of highly explosive triacetone peroxide that, in contrast to most explosives, 3 8

4 /

m/z

does not have any nitro groups, can be performed in a selective and sensitive manner using MSQ Plus.

TATP

Separation conditions

Analyte

Triacetone triperoxide (TATP) Flow rate 0.5

ml/min

TATP (222 m/z)

Mobile phase

binary gradient

+ Fragment (108 m/z)

1 mM ammonium formate/

+ Ammonium (18 m/z)

methanol

Injection volume 2

μl

Detector (MSQ)

APCI positive

Corona 30

μA

Temperature

Ion source 350 °C

8





Multi-components determi nation of toxicologically relevant compounds

The MSQ Plus allows sensitive Separation conditions

and robust quantifi cation of a Analytes see

table

multi-component sample over a Mobile phase

ternary gradient

H2O/MeOH/ACN

wide range of linearity. Compared 0.1% acetic acid

with conventional detection using Flow rate

1 ml/min

UV, the unique selectivity of lower Injection

2 μl

limits of quantifi cation and of Detector (MSQ)

ESI positive mode

detection are achieved for Capillary voltage 4.5 kV

individual analytes.

Temperature

Ion source 450 °C

Analyte

Limit of

Limit of

Linear

quantifi ca- detection

tion

ng/ml

ng/ml

ng/ml

1 Caffeine

2 Ephedrine

1.21

0.36

1.3–2000

3 Pseudoephedrine

1.25

0.38

1.3–1670

4 Amphetamine

1.78

0.53

1.3–1670

5 Hydrocodone

6.80

2.04

4.1–1000

6 Methampheta-

mine

0.96

0.29

1.3–1670

7 Oxycodone

3.48

1.04

3.3–10000

8 3.4-MDMA

1.09

0.33

1.3–1670

9 Clonazepam

7.39

2.22

3.3–3000

10 Cocaine

1.17

0.35

0.3–1000

11 Noscapine

3.79

1.14

0.7–10000

12 Cannabidiol

300

90.0

274–44400

13 THC

191

57.4

68.5–11100

14 Cannabinol

251

75.4

123–20000

9

Technical data Mass spectrometer

MSQ Plus

Installation requirements Power supply

Ionisation mode

U -��}�i «�>Ãi] ÓÎä 6H] ´£ä ¯] xäÉÈä â U ESI

Electrospray ionisation

U �ÃÌ>�Vi Ì� Ì�i �iÝÌ }À�Õ�` V���iVÌ���

U APCI Atmospheric-pressure less than 2 m

chemical ionisation

U «�ÜiÀ �ÕÌ�iÌ v�À Ì�i -+ *�ÕÃ

with a rated voltage of 15 A U ����Õ� Î «�ÜiÀ �ÕÌ�iÌà Ü�Ì�

Mass range

> Ì�Ì>� �����>� VÕÀÀi�Ì �v £Î ­ÓÎä6H®

U £Ç q Óäää �Éâ

Optimized for scans of molecules Gas supply

in the low mass range

U *ÕÀi] `ÀÞ >�` ����vÀii ��ÌÀ�}i� �à ÀiµÕ�ÀiÙ ����Õ� «ÀiÃÃÕÀi x L>À] ÀiµÕ�Àì purity greater than 99 %

Scan speed

U 1�ÌÀ>�v>ÃÌ ÃV>� ëiiÙ }i�iÀ>� ��ÌÀ�}i� ÃÕ««�Þ V>� Li ÕÃiàs well as nitrogen gas generators up to 12 000 amu/s

specified for this

U /�i ��ÌÀ�}i� ÃÕ««�Þ �ÕÃÌ Li >L�i to supply 700 l/h

Sensitivity

U � * ��ì Õ�ìÀ ��À�>� «ÀiÃÃÕÀi]

450 l/h are required

ESI positive ionisation

U �L��i «�>Ãi\ >ViÌ���ÌÀ��iÉÜ>ÌiÀ >Ì £ ��É���

U 1 , �vviÀà > ��ÌÀ�}i� }>à }i�iÀ>Ì�À ­ {£n®

that has been specially developed for the supply U ->�«�i ���« ���iVÌ���]

of LC/MS systems and generates a maximum 10 μl × 5 pg/μl erythromycin 1080 l/h with the pressures needed for this and UÊ -É \ xä «} iÀÞÌ�À��ÞV��] £äää\£ ,-

the required purity

ESI negative ionisation

Dimensions

U �L��i «�>Ãi\ �iÌ�>���ÉÜ>ÌiÀ >Ì £ ��É���

UÊ Îää Ý xÎä Ý Ç£ä �� ­7 Ý Ý ®

U ->�«�i ���« ���iVÌ���]

10 μl × 2 pg/μl p-nitrophenol Weights

UÊ -É \ Óä «} «���ÌÀ�«�i���] xää\£ ,-

UÊ - ìÌiVÌ�À\ >««À�Ý° Èä �}

U ,�Ì>ÀÞ «Õ�«\

APCI positive ionisation

45 kg typically standing on the floor U �L��i «�>Ãi\ >ViÌ���ÌÀ��iÉÜ>ÌiÀ >Ì £ ��É���

UÊ >Ì> ÃÞÃÌi� ­��V�Õ`��} ����Ì�À®\ � Îx �}

U ->�«�i ���« ���iVÌ���]

10 μl × 5 pg/μl erythromycin Environment

UÊ -É \ xä «} iÀÞÌ�À��ÞV��] Óää\£ ,-

U >Ý° �i>Ì `�ÃÃ�«>Ì��� �� À��� Î �7

U �L�i�Ì Ìi�«iÀ>ÌÕÀi �ÕÃÌ Li ÃÌ>L�i LiÌÜii�

APCI negative ionisation

15 °C and 35 °C

UÊ �L��i «�>Ãi\ �iÌ�>���ÉÜ>ÌiÀ >Ì £ ��É���Ê

U ,i�>Ì�Ûi �Õ��`�ÌÞ LiÌÜii� {ä ¯ >�` nä ¯

U ->�«�i ���« ���iVÌ���]

U � V��ì�Ã��} Ü>ÌiÀ Û>«�ÕÀ

10 μl × 2 pg/μl p-nitrophenol U /�i À�Ì>ÀÞ «Õ�« Ã��Õ�` ��Ì Li ��Ài Ì�>� £°x �

U -É \ Óä «} «���ÌÀ�«�i���] xä\£ ,-

away from the instrument and should be accessible for maintenance

* Selected ion monitoring (SIM) U � �ÕÌ�iÌ �à ÀiµÕ�Àì v�À Ì�i Ü>ÃÌi }>ÃiÃ

of the ion source and the rotary pump U /�i ìÛ�Vi Ã��Õ�` ��Ì Li �«iÀ>Ìì �� Ì�i Û�V���ÌÞ

of strong magnetic fields such as those originating from NMR devices, high voltage power lines, transformers or mass spectrometers with a magnetic sector

10





LC system

Accessories:

PLATINblue UHPLC

Nitrogen gas generator

Specifi cations

UÊ ��ÜÊÀ>Ìi\Êä°ä£qx°äÊ��É���Ê­xÊ��Ê«Õ�«Ê�i>`®

Specifi cations

UÊ >Ý°Ê«ÀiÃÃÕÀi\ÊÊ£äääÊL>ÀÊ­£xäääÊ«Ã�®ÊÌ�ÊÓÊ��É���

UÊÊ>Ý°ÊyÊ�Ü\Ê£nÊ�É���Ê­£änäÊ�É�®

800 bar (11600 psi) to 5.0 ml/min UÊÊ>Ý°Ê�ÕÌ«ÕÌÊ«ÀiÃÃÕÀi\ÊÈ°�ÊL>ÀÊ­£ääÊ«Ã�®

(5 ml pump head)

UÊÊ*>ÀÌ�V�iÊÃ�âi\Ê�Êä°ä£Ê��

UÊ ��ÜÊÀ>ÌiÊ>VVÕÀ>VÞ\ʴʣʯ

UÊÊiÀ�Ã��Ã\Ê �

UÊ ��ÜÊÀ>ÌiÊ«ÀiV�Ã���\Ê�Êä°£¯

UÊÊ*�Ì�>�>ÌiÃ\Ê �

Design/setup

PLATINblue HPLC Plus

UÊÊ"ÕÌ«ÕÌ\Ê£ÊÝÊ¥¸ÊÌ�Ài>ÙÊÊ�ÌiÀ�>�Ê>�ÀÊV��«ÀiÃÃ�À\Ê9iÃ]Ê��i HPLC Plus systems can be upgraded to complete UÊÊ*ÀiÃÃÕÀiÊ}>Õ}i\Ê9iÃ]Ê��i UHPLC systems at any time.

UÊ ��ÜÊÀ>Ìi\Êä°ä£q£äÊ��É���

Power supply

UÊ >Ý°Ê«ÀiÃÃÕÀi\ÊÊÇxäÊL>ÀÊ­£ännäÊ«Ã�®ÊÌ�ÊxÊ��É���

UÊÓÎä6H]ÊxäÉÈäÊâ]ÊΰÈÊ

400 bar (5800 psi) to 10 ml/min (10 ml pump head)

Dimensions

UÊ ��ÜÊÀ>ÌiÊ>VVÕÀ>VÞ\Ê´Ê£¯

U 40 x 71 x 70 cm (W x H x D) UÊ ��ÜÊÀ>ÌiÊ«ÀiV�Ã���\Ê�Êä°£¯

Weight

UÊÈäÊ�}

Sleep mode function

UÊÊ,ìÕViÃÊÌ�iÊÀÕ�Ì��iÊ�vÊÌ�iÊV��«ÀiÃÃ�ÀÊ>�ìncreases its life span Order number

A66500

MSQ Plus single quadrupole mass spectrometer

A69450

PLATINblue UHPLC/MS system A66501

Nitrogen gas generator N418LA A69420

PLATINblue UHPLC system, HPG, integr.

degasser, PDA-1 detector, autosampler, column thermostat

A69420PH PLATINblue HPLC Plus System, HPG, integr. degasser, PDA-1 detector, autosampler, column thermostat 11

Service Fast and reliable

In order to maintain the high 2 9�ÕÀÊ«iÀÃ��>�Ê*/ L�ÕiÊ-+ÊV��Ì>VÌÊ«>ÀÌ�iÀÊ

performance of your PLATINblue is responsible for all service features MSQ Plus for along time, we offer a service that guarantees smooth Support

2 On-the-job application advice – we support you operation. Here we present the during the running process with tips and tricks services that apply for the DE/AT/

from our experience

CH region. Please get in touch with your local KNAUER distributor to obtain a quote and discuss service options.

1 Y

W

ear

2 1 year guarantee

arranty

2 Installation service by your personal PLATINblue MSQ partner

Installation

& Training

www.platinblue.com

HPLC · SMB · Osmometry

Wissenschaftliche Gerätebau Telefon: +49-(0)30-809727-0

À°Ê�}°ÊiÀLiÀÌÊ�>ÕiÀÊ�L

Telefax: +49-(0)30-8015010

Hegauer Weg 38

E-Mail: info@knauer.net

14163 Berlin, Germany

Internet: www.knauer.net

^Ê 1 ,ÊÓ䣣Ê6Çn£{1-ÉΰäÉ£Ó°££É{/

Printed on paper awarded the EU Ecolabel EU Ecolabel : FI/11/1