How do you maximize purity and yield? 2 Continuous counter-current chromatography Get purity and yield! The SMB Principle In SMB processes you do not Feed ZONE 3 Raff Ra inate inat have to decide for either yield or purity – go for both! The high efficiency of SMB Column Swi allows for > 99% yield and tching purity simultaneously. ZONE 2 ZONE 4 Extract ZONE 1 Eluent Continuous counter-current chromatography, also In the classical SMB process, as it is illustrated in the known as Simulated Moving Bed Chromatography figure, the solid phase is divided into four separation (SMB) is a highly efficient separation technique in the zones by the use of the four inlets and outlets for feed, field of preparative chromatography. It enables binary or eluent, raffinate and extract. Zones 2 and 3 are the pseudo binary mixtures to be separated into pure separation zones and zones 1 and 4 regenerate liquid substances or fractions with maximum yield and purity. and solid phase. The different adsorption and desorption In an SMB process, a movement of the solid phase in a events that lead to the separation of the components counter-current direction to the movement of the liquid are controlled via the flow rates of the liquid phase in phase is simulated. In SMB processes the solid phase is the four separation zones and the switching time of the distributed over a number of columns. These columns columns. Depending on the application, normally are connected to each other in a closed circuit with 8, 12, or 16 columns are used. switchable column inlets and outlets. The feed mixture that contains the two components to What’s the difference between batch LC and SMBC? be separated is fed in the middle of these columns. The Batch LC SMB Chromatography component that has the higher affinity towards the solid phase travels in the direction, the movement of the solid Unlimited number of phase is simulated. The component that has the lower fractions Two fractions, no waste affinity towards the solid phase travels with the liquid Recovery typically < 80% Recovery up to 100% phase. So you can withdraw the pure substances at the EITHER high purity OR extract and raffinate ports. The movement of the solid is high yield High purity AND high yield simulated by switching the columns counter-clockwise at defined timed intervals, creating a quasi-continuous Isocratic or gradient Isocratic (step gradient possible) process. Recycling possible Recycling is part of the in isocratic mode process (up to 90%) High solvent consumption Can be as low as 1/10th of batch consumption Product concentration Strongly diluted product comparable with input concentration (Feed) > Versatile technique > Highly efficient technique 2 Multi-function valve for column switching Very small hold-up volume ✓ Made of inert Hastelloy ✓ Different valve seals for a wide range of applications ✓ 3 What makes us attractive? It’s about the inner values. KNAUER SMB chromatography systems are very They come with user-friendly control software reliable due to the use of high quality components and ChromGate® SMB. All KNAUER SMB systems can be materials. equipped with the efficiency enhancement Modicon® which can boost high SMB productivity by up to 50%. The modular design makes them adaptable to different tasks, while preseving your investment. The systems feature a very small hold-up volume for as little as possible losses of valuable product. Rack with pumps and valves SMB control unit Multi function valve Column compartment SMB System CSEP® C9812 Production Unit The system is designed for the extraction of pure substances in the kilogram-scale. Tubing 1/8”, pressure up to 50 bar, supports up to 12 columns, heatable compartment with leak detection. SMB unit complete with control software and PC. 4 C9812 C9116 System specifications System specifications Max flow rate (ml/min) 500 Max flow rate (ml/min) 50 Multi-function valve 1 x 48-Port Multi-function valve 1 x 64-Port Available pump heads Available pump heads (ml/min) 100, 250, 500 (ml/min) 10, 50 Column dimensions (max.) 1 000 mm x 32 mm ID or Column dimensions (max.) 300 mm x 30 mm ID 150 mm x 62 mm ID Pressure rating 50 or 100 bar Pressure rating 20 or 50 bar Number of columns with Number of columns 4, 6, 8, or 12 columns inner diameter 16 mm 4, 8, 12, or 16 columns Total column weight max. 120 kg Number of columns with Temperature control max. 60 °C inner diameter 30 mm 4 or 8 columns Pump Type Smartline Pump 1800 Total column weight max. 20 kg Flow meter for Temperature control max. 60 °C flow rate monitoring 2 x Coriolis Pump Type Smartline Pump 100 Tubing, fittings and 1/8“ stainless steel Flow meter for flow accessories (PEEK upon request) monitoring 2 x Coriolis Application Area Kilogram-scale production Tubing, fittings and 1/16" stainless steel Footprint approx. 125 x 70 cm accessories (PEEK upon request) Control system PC with control software Application Area Process development, gram-scale production Footprint approx. 110 x 50 cm Control system PC with control software Ordering information Order No. Software A28701 CSEP® C9116, up to 16 columns, 16 mm ID A28706 CSEP® C9812, up to 12 columns, 62 mm ID A28703 ModiCon Option for CSEP C9116 SMB Systems includes the ModiCon license fee for 5 years, an update of ChromGate® control software, and a low pressure gradient unit for the feed pump A28704 CSEP® C9812, ModiCon Option Are you into bio downstream processing? Biocompatible configurations are available for purification of peptides and proteins. Stainless steel is hereby replaced by titanium alloy and PEEK. Glass columns are available to complete the biocompatible setup. 5 System control The KNAUER SMB systems can be controlled by ChromGate® SMB, which is based on the well-known EZChrom Elite kernel. Usage is as easy as with analytical and preparative HPLC systems. The integration into KNAUER’s ChromGate chromatography data system gives access to We also offer non-standard systems on request. We drivers for a wide range of additional instruments design a tailor-made system according to your prefe-for individual setups. rence and purification task. SMB System CSEP® C9116 Pilot Unit The system is designed for the extraction of pure substances in the gram-scale. Tubing 1/16”, pressure up to 100 bar, supports up to 16 columns, heatable Keep track of pressures, flow rates, temperature, leak events compartment with leak detection. SMB unit complete with control software and PC. ChromGate® SMB: easy configuration and control of the SMB system Why not boost SMB productivity even further? In the classic SMB technique, the mixture to be separated is fed at a constant concentration. With the ModiCon technique, throughput and purity can be further increased by modulating the inlet concentration. Developed by the Max Planck Institute for Dynamics of Complex Technical Systems (Magde-burg, Germany), the ModiCon technique is being distributed exclusively by KNAUER. New systems as well as already existing ones can be equipped with the ModiCon option. The Modicon feature is available for all KNAUER SMB systems. It optimizes feed concentration for up to 50% higher productivity and up to 25% less solvent use. 6 Separation impossible? Strongly overlapping components, which can hardly be purified using preparative batch HPLC, pose no separation problem to SMB. This is made possible through the internal recycling nature of the process, which virtually extends the column length as required and keeps losses concentration to a minimum. time How important are time, space, and operating costs for you? Work faster Save up to 90% of solvent Due to the continuous “injection“ (feed) and separation In SMB, most of the solvent is regenerated and recycled in SMB there are no such things as “runs“ or “latency inside the process. You can save up to 90% of the between runs“. In SMB “operation time“ equals solvent compared to batch processes. “separation time“. The result is much higher productivity than possible with batch LC. Get your targeted amount of purified product in less time! Get your undiluted product Use columns and Batch chromatography leads to strong dilution of the target component – a factor of 20 is typical. space more efficiently The chromatographic step usually needs to be followed by concentration steps. In SMB, outlet concentration can reach inlet concentration. Use SMB to gain undiluted In SMB processes the stationary phase is used more products and minimize concentration efforts. efficiently than in batch LC. While in batch runs only 10–20% of the column bed are loaded, in SMB up to 100% of the bed in the separation regions are loaded with substance. This not only makes better use of packing material, but also enhances productivity. Instead of running multiple batch LC stations in parallel, one SMB does the job, which saves space. 7 8 8 Why KnAUER SMB? KnAUER SMB systems are characterized by their modular setup. This makes them flexible and allows for individual configurations. Central element of the KNAUER CSEP® SMB system is the multifunction valve made of Hastelloy which connects the inlets and outlets of the columns with the pumps. Since only one valve is required for column switching, the KNAUER SMB system is particularly compact and uncluttered. Flexible KNAUER SMB comes on a trolley rack and requires only standard power supply. The system dead volume is significantly lower than that of systems with several valves. In addition, service expenses are also minimized. The multi-function valve and columns are positioned inside an oven, enabling operation of columns from room temperature up to 60°C. Column compartment of the CSEP® 9116 Unit How adaptable should your SMB solution be? The KNAUER SMB system can easily be modified to apply other cyclic purification processes involving multiple columns, e.g. multi-step cleaning. Eluent Extract Eluent Extract Equilibration Waste Column S Colu w mn it S c w h it in ch g ing Waste Wash Raffinate Feed Waste Feed Isocratic SMB Process Cyclic multi-step processing 9 Looking for professionals? KnAUER columns and method development service With our experience in manufacturing analytical and preparative HPLC columns, we can supply sets of columns with outstanding similarity in a wide range of stationary phases. KNAUER’s high pressure glass columns with adjustable bed length provide the user with the opportunity to change the resin quickly and are also ideal for separating biomolecules. Our application lab offers the screening of stationary phases as well as detailed method development studies. These services reduce your effort in method development and ensure that you get optimized equipment for your separation. Talk to our specialists Call +49 30 809727-111 www.knauer.net HPLC · SMB · Osmometry Wissenschaftliche Gerätebau Telefon: +49 30 809727-0 Dr. Ing. Herbert Knauer GmbH Telefax: +49 30 8015010 Hegauer Weg 38 E-Mail: info@knauer.net 14163 Berlin, Germany Internet: www.knauer.net 10 (C) KNAUER 2012 V7706US/1.0/04.12/4T Printed on paper awarded the EU Ecolabel EU Ecolabel : FI/11/1