CIRAS-3 Portable Photosynthesis System • Photosynthesis • Chlorophyll Fluorescence • Soil Respiration • Canopy Assimilation www.ppsystems.com CIRAS-3... The New Sta Adjustable handle 27 tactile keys for user inputs and system navigation Optimized viewing angle (30°) Large, full color transflective LCD Rugged, lightweight aluminum enclosure Easy access battery compartment Polyurethane, shock absorbing base CIRAS-3 Main Console Weight: 4.3 kg (including battery) Dimensions: 28.0 cm (W) x 14.5 cm (D) x 24 cm (H) www.ppsystems.com 2 sales@ppsystems.com andard For Field Research External PAR sensor Infrared leaf (Ambient) temperature sensor On-board LCD Switch display parameters Remote recording PAR sensors (Internal) Peltier cooler (Temperature control) Ambient temperature sensor Interchangeable Head Plates for PLC3 Universal Leaf Cuvette PLC3 Universal Leaf Cuvette Weight: 0.75 kg 25 mm x 7 mm 18mm Diameter 25 mm x 18 mm Dimensions: 32 cm (L) x 3.8 cm (Handle diameter) Area: 1.75 cm2 Area: 2.50 cm2 Area: 4.5 cm2 www.ppsystems.com 3 sales@ppsystems.com Experience Pioneering Leaf Gas 3rd Generation Exchange Measurements Photosynthesis System Our Proud History For High Level Research PP Systems is considered a world leader CIRAS-1 (1992) in the manufacture and design of 1st portable system to feature integral instrumentation for high level plant control of CO and soil science research. Our experience 2 (using mini CO2 cartridges) and H in photosynthesis measurements over the 2O. Soon thereafter we introduced a range of leaf cuvettes that included past 30+ years has yielded an instrument automatic control of temperature and that includes state of the art design and light for response curves. unsurpassed performance without sacrificing portability. Many of the features that have been incorporated into the CIRAS-3 system are the result CIRAS-2 (1999) of numerous discussions and research performed by some of the world’s most 1st portable system to feature prominent photosynthesis experts over integral, simultaneous measurement many years. of photosynthesis and chlorophyll fluorescence. The CIRAS-3 is the perfect choice for measurement of: • Photosynthesis CIRAS-3... The Evolution Continues • Chlorophyll Fluorescence Experience true portability • Soil Respiration • Canopy Assimilation Our Mission Aiding scientific research with versatile, proven technology. PP Systems is proud to have supported the technology needs of plant and soil scientists since 1984. Our photosynthesis systems have been proven and tested by thousands of customers • Small footprint and lightweight (just 4.3 kg) from over 100 countries worldwide. We are recognized • Internal,rechargeable Li-ion battery providing up to 8 hours use as a leader in the design and • 4 independent,non-dispersive infrared gas analyzers for both CO2 and H2O manufacture of photosynthesis • Automatic control of CO2,H2O,temperature and light measurement instrumentation for high level research. • Simultaneous measurement of photosynthesis and chlorophyll fluorescence • Large,full color 7.0”transflective LCD for field use with optimized viewing angle • Unlimited data storage (internal memory and use of thumb drives) Trusted and tested technology • Flexible and versatile user interface • Powerful software and programming capability www.ppsystems.com 4 sales@ppsystems.com CIRAS-3 - Main Console As reported by many researchers, there is a big difference between “portable” and “transportable”. At PP Systems, our goal has always been to provide researchers with the most portable system possible without sacrificing performance. Our reputation for doing just that is unsurpassed. The light weight (4.3 kg) and small footprint of the CIRAS-3 console means reduced fatigue, less site disturbance of fragile vegetation, and access to “hard to reach” places. The console is constructed out of a rugged, ergonomic aluminum enclosure with custom designed polyurethane cover and shock absorbing base. The system is powered by a very efficient and powerful internal Li-Ion battery providing up to 8 hours operation in the field eliminating or reducing the requirement to change out batteries. The CIRAS-3 console can be used as a stand-alone CO2 and H2O differential gas analyzer for use with custom chambers in the laboratory or field if required. Quickly change between open and closed system analysis for measurement of soil CO2 efflux and net canopy CO2 flux. CO2/H2O Gas Analysis A major part of any photosynthesis system is the gas analyzer. Accurate, reliable and stable measurement and control of CO2 and H2O is critical for high level research. The CIRAS-3 is a “true differential analyzer” featuring 4 independent, non-dispersive gas analyzers for accurate, simultaneous measurement and control of both CO2 and H2O. For high level research and greatest accuracy, this is a significant advantage over “gas switching” systems and a major requirement for most researchers. For enhanced reliability, there are no moving parts such as chopper motors or filter wheels. The gas analyzers include an infrared source, highly polished and gold plated sample cells and detectors that are optimized for CO2 (4.26 µm) and H2O (2.60 µm). The analyzers act as absorptiometers measuring infrared absorption only. The optical bench is temperature controlled and pressure compensated ensuring the most accurate CO2 and H2O measurements under changing ambient conditions. Measurement Ranges • CO2: 0-10000 µmol mol-1 • H2O: 0-75 mb Calibration, Accuracy and Long Term Stability The design of the CIRAS-3 ensures an inherent calibration stability that has been confirmed by over 30 year’s experience in gas analysis technology. The CIRAS-3 features an innovative Auto-Zero function that allows for fast warm-up, adaptation to changing ambient conditions and excellent stability. In short, you can expect your calibration to be accurate, reliable and stable for many years without the need for time consuming, expensive recalibration. The Auto-Zero function minimizes effects on span (gas sensitivity) of sample, source aging, changes in detector sensitivity and changes in pre-amplifier gain. Periodic system checks are simple and recommended to confirm system integrity and calibration. www.ppsystems.com 5 sales@ppsystems.com Environmental Control – CO2, H2O, Temperature & Light The level of environmental control in the field with the CIRAS-3 is unsurpassed. The CIRAS-3 offers complete, independent and automatic control of CO2, H2O, temperature and light for response curves (i.e. A/Ci curves, light response, etc.) with fast time response. Control of all environmental parameters can be dynamic or preprogrammed for automated response curve generation. All power required for environmental control is provided by the internal Li-Ion battery (no external power supply is required). CO Replaceable CO 2 Control 2 cartridge Unlike some systems on the market today, automatic CO2 control is standard with the CIRAS-3. Our innovative, integral gas blender and regulator mixes pure CO2 provided by mini CO2 cartridges Desiccants for zero and CO (8 g) with CO 2 /H2O control 2 free air to provide a very accurate, continuous and constant flow of CO2. Each CO2 cartridge is capable of providing at least one full day of operation. It is very easy to change out H2O Control cartridges and there is little to no maintenance required for the On-board, self-indicating desiccants are used for conditioning the CO2 gas blender or regulator. H • 2O concentrations per user selected levels. CO2 Control Range: 0-2000 µmol mol-1 • H2O Control Range: 0-Dewpoint The system can easily be configured for ambient measurement The system can easily be configured for ambient measurement of of CO2 if required. H2O if required. Temperature Control LED Light Unit (RGBW) Automatic, accurate and stable temperature control is standard and integral with all leaf cuvettes. Peltier coolers are mounted to the cuvette head along with heat sink and fans for precise control of temperature over a wide range. Several controlling features can be selected by the user including cuvette temperature, leaf temperature, tracking cuvette to ambient and tracking leaf to ambient. Temperature control can also be disabled if required. • Temperature Control Range: ~ 10 oC below ambient to 15 oC above ambient • Temperature Control Limits: 0-45 oC Peltier Cooler Light Control (Optional) All leaf cuvettes can be supplied with an optional, compact LED light unit for automatic control of light. Each LED light unit features a bank of red, green, blue and white LEDs (RGBW) allowing for control of both light intensity and proportion of red, green, blue and white light. • Light Control Range: 0-2500 µmol m-2 s-1 (range varies depending on light unit type) Controlling light intensity up to 2500 µmol m-2 s-1 allows oversaturating and induced photoinhibition replicating Environmental Control Setup Screen extreme light environments (i.e. high elevation, desert). www.ppsystems.com 6 sales@ppsystems.com Leaf Cuvettes The CIRAS-3 can be supplied with 3 standard leaf cuvettes (Universal, Narrow and Conifer) which are truly plug and play. Each cuvette can be connected directly to the CIRAS-3 without the need for time consuming, delicate reassembly and adjustment of different heads and sensors. All leaf cuvette materials are carefully selected to minimize influences such as infrared radiation, water sorption, CO2 effects and leaks. The leaf cuvette gaskets provide an air-tight seal without causing any damage to vegetation. Automatic temperature control is standard with all leaf cuvettes. PLC3 Universal … For flat, broad leaves Our PLC3 Universal Leaf Cuvette is our most popular leaf cuvette by far. The PLC3 Universal is designed for measurement on most flat, broad leaf plants. It features 3 different windows allowing you to customize the head to accommodate different sized leaves. • Window: IR Filter • Window Area: 25 mm x 7 mm / 18mm Diameter / 25 mm x 18 mm The PLC3 Universal Cuvette includes 2 miniature silicon photodiode sensors providing a reliable average of PAR inside the cuvette in addition to ambient PAR measured by an external, cosine corrected PAR sensor. It also includes an infrared radiation sensor for accurate, non-contact measurement of leaf temperature. Leaf temperature can also be calculated based on energy balance. PLC3 Narrow … For grasses, long needles and narrow leaves For measurement on grasses, cereal crops and long needle conifers. • Window: IR Filter • Window Area: 83 mm (L) x 30 mm (W) The PLC3 Narrow Cuvette includes 1 miniature silicon photodiode sensor providing measurement of PAR inside the cuvette in addition to ambient PAR measured by an external, cosine corrected PAR sensor. Leaf temperature is determined by use of a leaf thermistor or calculated based on energy balance. An optional head plate can be supplied to easily convert from PLC3 Narrow to Conifer. PLC3 Conifer … For conifers and short needle vegetation For measurement on grasses, cereal crops and short needle conifers. The window is hemispherical making it suitable for 3D structures. • Window: Glass • Window Area: 83 mm (L) x 40 mm (Height) The PLC3 Conifer Cuvette includes 1 miniature silicon photodiode sensor providing measurement of PAR inside the cuvette in addition to ambient PAR measured by an external, cosine corrected PAR sensor. Leaf temperature is determined by use of a leaf thermistor or calculated based on energy balance. An optional head plate can be supplied to easily convert from PLC3 Conifer to Narrow. www.ppsystems.com 7 sales@ppsystems.com LED Light Unit - LED Light Unit (RGBW) RGBW (Optional) An innovative, compact, RGBW light unit is available for each leaf cuvette as an optional accessory. For field use, the LED based light source is ideal because it features low power consumption, minimal heat generation and can easily be installed or removed from the cuvette head. This is highly recommended for users that want to perform light response curves either manually or automatically. Not only can it automatically control light intensity up to 2500 µmol m-2 s-1 (range varies depending on light unit type), the user can also control the proportion of red, green, blue and white light in any combination up to 100%. The light source can also be PLC3 Universal Light Unit removed for ambient measurements if required. • RGBW Specification Automatic control of light intensity up to 2500 µmol m-2 s-1 Red Peak Wavelength: • Select red,green,blue or white light and any 625 nm +/- 5 nm combination up to 100% Green Peak Wavelength: 528 nm +/- 8 nm • Uniform light distribution within the chamber Blue Peak Wavelength: • Lightweight and simple connection to the leaf 475 nm +/- 10 nm cuvette head White: 425-700 nm Leaf Cuvette LCD Display All leaf cuvettes include an on-board LCD display simplifying field measurements. Researchers can display user defined parameters on the 2 x 16 character display. It can display up to 4 parameters simultaneously but users can toggle between different displays for more parameters. This is ideal in those “hard to reach” sampling locations that prohibits users from reading the console. Having the data on the cuvette LCD allows the user to focus on the vegetation and it also lets them know when readings are stable and ready to be recorded. Two tactile feel keys are available on the cuvette to allow the user to toggle the LCD and to record measurements remotely. www.ppsystems.com 8 sales@ppsystems.com User Interface & Software The CIRAS-3 main console incorporates a large, full color 7.0” WSVGA transflective display featuring superb readability under ambient conditions especially under high sunlight. The display is custom designed with a 30° viewing angle making it easier to read from various positions ensuring measurements can be performed and recorded by just one person. A custom, 27 tactile feel keypad is available for all user inputs and system navigation. User Friendly Simple, intuitive, menu-driven controls for setup, operation, recording routines and management of data are all designed into the graphical user interface (GUI). On-line system help is available to guide you every step of the way. Data Presentation Users can customize the information presented on the LCD. Many of the displays and presentation of data are user defined. Data Collection and Transfer Measured and calculated photosynthesis and chlorophyll fluorescence parameters With just a few key presses data files can be created and measurements executed and saved to internal memory or to a convenient USB thumb drive (memory stick). Data storage is flexible and unlimited and can easily be transferred to a PC for further analysis. USB thumb drive used to upload/download data Measured and Calculated Photosynthesis Parameters CO2 reference, CO2 analysis, CO2 differential, H2O reference, H2O analysis, H2O differential, air temperature, leaf temperature, cuvette temperature, PAR internal, User defined graphics/scatter plots PAR external, relative humidity, atmospheric pressure, flow, Ci, gs, A, E, VPD and WUE Response Curves Response curves can easily be executed manually or automatically. Users can either load factory default response curve scripts or they can modify, edit or create their own. Response curve scripts Graphical display setup Response Curve Scripts Editor can also be shared between users making it easy to duplicate experiments. A series of A/Ci curves on Arundo donax (Giant Cane) at various leaf development stages View saved data www.ppsystems.com 9 sales@ppsystems.com CFM-3 Chlorophyll Fluorescence Module (Optional) The CFM-3 Chlorophyll Fluorescence Module is an optional accessory for use with the PLC3 Universal Leaf Cuvette for simultaneous CFM-3 Chlorophyll measurement of photosynthesis and chlorophyll fluorescence. Fluorescence Module Independent measurement of chlorophyll fluorescence is also possible. The CFM-3 is compact and elegantly designed with all light sources and fluorescence detection capability built directly into the light unit. The CFM-3 light unit can be used as an actinic light source for leaf gas exchange and as a pulse-amplitude modulated (PAM) fluorometer for measurement of chlorophyll fluorescence on both dark and light adapted vegetation. The CFM-3 is capable of delivering saturating pulses up to 10000 µmol m-2 s-1 which is extremely useful in accommodating a wider range of ambient light conditions. The system is also capable of producing a “Multi-Pulse” sequence of saturating light levels for estimation of apparent Fm’. Leaf cuvettes can also be modified for use with commercially available fluorometers. Measured and Calculated Chlorophyll Fluorescence Parameters Fo, Fv, Fm, Fs, Fo’, Fm’, Fv/Fm, 0PSII, J, Fv’/Fm’, qP, qNP, NPQ, qL, 0NO, 0NPQ-K, 0fD and 0NPQ-G Photosynthesis and chlorophyll fluorescence data can be displayed on one screen. Graphical presentation of data is also available. PC Utility Software & Remote Display Programming simple or complex experiments from a PC could not be any easier. Users can create their own response curve scripts using the popular XML programming language. Once created, programs can be downloaded to the CIRAS-3 or shared with other researchers that might want to replicate measurements. Data can also be displayed remotely from a PC making it ideal for teaching and demonstration purposes. Response Curve Scripts Editor Remote Display www.ppsystems.com 10 sales@ppsystems.com Soil Respiration and Net Canopy CO2 Flux Soil CO2 Efflux Net Canopy CO2 Flux The popular SRC-1 Soil Respiration Chamber can be used with Our CPY-4 Canopy Assimilation Chamber is ideal for measurement the CIRAS-3 for rapid, accurate measurement of closed system of net canopy CO2 flux on low lying vegetation. soil CO2 efflux. The chamber is transparent and includes sensors for measurement The chamber is constructed out of rugged PVC with a stainless of PAR and air temperature. steel ring providing a good seal. Headquarters Situated in the heart of New England, PP Systems is headquartered in the Boston North Technology Park (BNTP) which is located in Amesbury, MA (USA). Training & Support Our track record for offering the highest level of technical support is second to none. All new customers are eligible to receive a FREE training course on the CIRAS-3 Portable Photosynthesis System. Our experienced technical staff provides full product training at our Amesbury, MA (USA) factory several times each year. Class size is small to guarantee that each customer receives the personal attention required and deserved. Customers come away from the course feeling well trained and For international visitors, Boston’s Logan International airport comfortable with all aspects of the system from an is most convenient and is approximately a 40 minute drive to operational and maintenance standpoint. Amesbury. If you are ever in our area, please feel free to stop in for a visit. www.ppsystems.com 11 sales@ppsystems.com Technical Specification Main Console PLC3 Leaf Cuvette Analysis Method Auxiliary Port Construction Non-dispersive infrared, configured as an For connection to external devices (i.e. soil Handle: Aluminum absolute absorptiometer with microprocessor respiration chamber, canopy chamber). Leaf Gasket: Closed cell foam control of linearization. Four independent gas Digital Output Impeller: Stainless Steel analyzers simultaneously measure absolute CO2 • USB-Mini b (Host) Window and H2O for both the reference and analysis • 2 Ea. USB for use with external devices PLC3 Universal: Glass IR interference filter gas streams. All measurements corrected for (mouse, memory stick). PLC3 Universal Window Sizes temperature and pressure. Data Storage • 25 mm x 7 mm (1.75 cm2) CO2 Measurement Range 512 MB flash memory for programming and data • 18 mm Diameter (2.5 cm2) • 0-10000 µmol mol-1 storage. Unlimited data storage using USB thumb • 25 mm x 18 mm (4.5 cm2) CO2 Precision drives (memory sticks). PLC3 Narrow: Glass IR interference filter • 0.2 µmol mol-1 at 300 ppm Microprocessor Speed PLC3 Conifer: Scratch resistant glass • 0.5 µmol mol-1 at 1,750 ppm 800 MHz LCD Display • 3.0 µmol mol-1 at 10000 ppm Display 2 x 16 character LCD for display of user CO2 Control Range 7.0” WSVGA transflective color LCD defined parameters • 0-2000 µmol mol-1 User Input Keypad H2O Measurement Range 27 tactile keys. Two tactile feel keys for recording and • 0-75 mb LCD selection Power Supply H2O Precision Internal, rechargeable 7.2V Li-Ion battery PAR Sensor (Internal) • 0.015 mb at 0 mb providing up to 8 hours continuous use. PLC3 Universal: 2 Miniature, silicon • 0.020 mb at 10 mb Power supply/charger included. photodiode sensors • 0.030 mb at 50 mb PLC3 Narrow: 1 Miniature, silicon Operating Temperature Range H photodiode sensor 2O Control Range 0-50 oC, non-condensing. In dirty environments, • 0-Dewpoint. PLC3 Conifer: 1 Miniature, silicon external air filtration may be required. photodiode sensors Pressure Range Enclosure • Response: 400-700 nm • 65-115 kPa Rugged, ergonomic, lightweight aluminum with • Range: 0-3000 µmol m-2 s-1 Air Sampling polyurethane base. • Precision: 10 µmol m-2 s-1 User adjustable from 50-100 cc min-1 using Dimensions PAR Sensor (External) Filtered silicon cell integral DC pumps. Both analysis and reference 28 cm W x 14.5 cm D x 24 cm H (cosine corrected) pumps fitted with mass flow controllers. Weight • Response: 400-700 nm Cuvette Air Supply Unit 4.3 kg • Range: 0-3000 µmol m-2 s-1 0-500 cc min-1 measured and controlled by • Precision: 10 µmol m-2 s-1 a mass flow meter. Air Temperature Sensor • Range: -10 oC – 50 oC • Precision thermistor • Accuracy: +/- 0.5 oC at 25 oC Distributor CFM-3 Chlorophyll Temperature Control Fluorescence Module ~10 oC below ambient to +15 oC above ambient. (Optional) Temperature Control Limits: 0 oC to 45 oC. Leaf Temperature Sensor Modulating Beam 625 nm +/- 5 nm (Red) PLC3 Universal: Radiation sensor for non-contact Saturation Light 0-10000 µmol-2 s-1 measurement Far Red Light 2 x 750 nm LEDs • Accuracy: +/- 0.5 oC at 25 oC Detector PIN Photodiode with PLC3 Narrow and Conifer: Precision Thermistor >700 nm filter • Accuracy: +/- 0.5 oC at 25 oC Detector Method Rapid pulse Dimensions peak tracking 32 cm (L) x 3.8 cm (Handle diameters) Leaf Area 1.75 / 2.5 / 4.5 cm2 Weight: 0.750 kg 110 Haverhill Road • Suite 301 PP Systems is a registered trademark of PP Systems, Inc. Amesbury, MA 01913 U.S.A. PP Systems is continuously updating its products and TEL +1 978-834-0505 reserves the right to amend product specifications FAX +1 978-834-0545 without notice. EMAIL sales@ppsystems.com All brand names are trademarks or registered trademarks URL www.ppsystems.com of their respective owners. Printed: June 2013 Copyright © PP Systems 2013 www.ppsystems.com All rights reserved.