RiboFlow™ Cronobacter For safer infant formula Reliable Simple Rapid Safe Economic Medalled technology safer infant formula The genus Cronobacter described in 2008 comprises six species: C. sakazakii C. malonaticus C. muytjensii C. dublinensis C. turicensis C. genomospecies 1 Known as neonatal pathogens, Cronobacter spp. are regarded as causative agents of meningitis, septicaemia or necrotising enterocolitis in infants with a rather high mortality. Contaminated powdered infant formula has been identified as the most likely vehicle of transmission for Cronobacter spp. associated with hospital outbreaks. With RiboflowTM Cronobacter SY-LAB provides a rapid and accurate detection system for Cronobacter spp. Thus enabling an efficient in-process-control of manu-facturing plants as well as a powerful risk assessment and -management with respect to Cronobacter relevant food borne diseases. your benefits Reliable Proven molecularbiological technology Novel rRNA based highly specific pathogen detection system. Simple Simple to perform. No instrumentation necessary. Rapid Results available in < 30 hours. Safe Unsurpassed specificity; Enrichment broth without Laurylsulfate and Crystal Violet. No cross reaction from Enterobacteriacea competitors. Economic No capital costs. Minimal hands on time. Affordable rapid assay costs. test procedure 1. Standard sample enrichment in Bufferd Peptone Water at 37° C for 16-20 hours. 2. Selective enrichment in SY-LAB Cronobacter Enrichment Broth (CEB) at 42° C for 12-20 hours. 3. Transfer 0,5 ml of the CEB enrichment to Micro-centrifuge cups and centrifuge for 5 minutes. 4. Discard supernatant, add lysis and denaturation reagents. 5. 5 min incubation at room temperature followed by addition of 60 µl running buffer. 6. Transfer 100 µl to a RiboFlowTM Cronobacter test device, incubate at 37° C and read results after max. 15 minutes. typical results: Negative test result (l.) Positive test result (r.) Note A combined protocol for the use with the BacTrac 4300 impedance analyzer is also available. The test can also be used to confirm suspicious colonies from agar plates within 3-5 hours. ordering information 51-416113 RiboFlowTM Cronobacter 24 Tests 41-441450 Cronobacter Enrichment Broth, 60 vials a 9 ml incl. Vancomycin 41-430012+ Screw caps, white, sterile 40 pcs/pack. – We develop solutions for a safer future! – SY-LAB Geräte GmbH Tullnerbachstraße 61-65 3011 Neupurkersdorf AUSTRIA www.sylab.com sales@sylab.com +43-2231-62252-0 Version 2.0 gb