MICROBIOLOGY

AMP-6000®

Enumeration of

Cheese Spoiling

Clostridia*

SY-LAB – THE NEW STANDARD

FOR A SAFER CHEESE PRODUCTION

Butyric acid producing Clostridia are Clostridia represent strictly anaerobic Sensitivity:

undesired contaminants in cheese gram-positive rods capable to form Limit of Quantification:

production due to their ability to cause extreme resistant endospores. Clostri-75 Spores/Liter standard microplate late-blowing, a severe quality defect in dial spores can withstand very high 30 Spores/ Liter deepwell plate long ripened hard and semi-hard and/or low temperatures and can‘t be cheese.

inactivated neither by extreme pH-Specificity:

Contaminated raw milk is the major values nor by irradiation. They are able highly specific detection of

source of Clostridia. Less then 100 to colonise many different habitats Clostridium tyrobutyricum, spores per liter of raw milk may result with strong seasonal variations. They Cl. butyricum, Cl. sporogenes and in severe cheese deficiencies like irre-can ideally replicate in silage and are Cl. beijerinkii.

gular eye formation, slits and cracks or able to passage the ruminant intestine efficient suppression of competing unpleasant off-flavors and therefore without any damage. Raw milk is microflora compared to conventional cause severe economic losses.

contaminated via the fecal route.

reference methods.

Clostridia enumeration is therefore a The 4 most common species isolated valuable parameter to control hygienic from milk and cheese are Clostridium conditions in stables of dairy cows.

Matrix:

tyrobutyricum, Clostridium butyricum, Additionally it can be used as an indica-Milk and Cheese

Clostridium sporogenes and Clostridium tor for the type of roughage used to beijerinckii. Clostridium tyrobutyricum feed the cows.

has been described as the major source of late blowing.

The AMP-6000® microbiological plat-form enables precise enumeration of cheese spoiling Clostridia by a new miniaturised and semi-automated MPN- technology.

* patent pending

RAPID:

PRECISE:

SIMPLE:

Results within 48 hrs.

reliable and comparable

automated sample preparation

enumerations

and result evaluation

SY-LAB Geräte GmbH • Tullnerbachstraße 61-65 • 3011 Neupurkersdorf, Austria • www.sylab.com • sales@sylab.com





MICROBIOLOGY

Workflow

Sample pasteurisation

Sample application and

Anaerobic Incubation

Colour scan and calcu-

(20 min.)

automated serial dilution

(48 hrs.)

lation of results (apprx.

(apprx. 2 min./ sample)

1 min per sample)

Consumables for the enumeration of cheese spoiling Clostridia Standard protocol (Detection limit > 75 spores/liter)

• 62-166613

AmpMedia 666 box with 60 vials for 180 tests in total

• 62-505095

Sample reservoir 25 ml, sterile, pack for 180 tests

• 62-503060

Microtiter plates and lids, sterile for 60 tests

• 62-502200

Pasteursation vial with filter, sterile 60 vials

• 62-504016

Pipette tips for AMP-6000® APS, sterile, 16 packs a 96 tips for 192 tests Protocol for enhanced sensitivity (Detection limit > 30 spores/liter)

• 62-166613

AmpMedia 666 box with 60 vials for 60 tests

• 62-505015

Sample reservoir 50 ml, sterile, pack für 60 tests

• 62-502110

Deepwell plates and gas permeabel seals, sterile for 100 tests

• 62-502200

Pasteursation vial with filter, sterile 60 vials

• 62-504016

Pipette tips for AMP-6000® APS, sterile, 16 packs a 96 tips for 192 tests Accessories

• 60-600160 Petrisphere Gas System

• 60-600170 Anaerobic jar, stainless steel with rack für 15 microplates or 8 deepwell plates

• 60-600150 Mini-Shaker for microplates SY-LAB Geräte GmbH • Tullnerbachstraße 61-65 • 3011 Neupurkersdorf, Austria • www.sylab.com • sales@sylab.com