bbe biological •biophysical •engineering moldaenke D a p h n i a T o x i m e t e r I I A powerful instrument for water toxicity assessment continuous visual analysis of daphnia behaviour w w w . b b e - m o l d a e n k e . d e D a p h n i a T o x i m e t e r I I b b e m o l d a e n k e © Mobility and analysis of daphnia Speed: average velocity and distribution α D = 1.09 Fractal dimensions: curviness and angle Distance and grouping Swimming height Daphnia growth D a p h n i a T o x i m e t e r I I i n t h e l a b Shading: time index and distribution The bbe Daphnia Toximeter II observes daphnia online Daphnia are established test organisms to indicate water quality. The bbe Daphnia Toximeter II observes daphnia under the influence of constantly running sample water. bbe developed the 24/7 sensitive method to detect hazardous compounds in water from rivers (source-water protection), plants, distribution systems and production drains to preserve human health and to monitor water. The instrument is also designed as an early warning system to rapidly detect the entire range of dissolved toxic compounds including pesticides, neurotoxins and warfare agents. Thus it is well suited to detect wilful (terrorist attacks) or negligent incidents (spills, accidents). The bbe Daphnia Toximeter II can also be used for long-term monitoring for the “strategic” evaluation of water quality and as a valuable tool in hazard management. The bbe Daphnia Toximeter II has been deployed worldwide now for over 10 years. Measurements The method of image analysis enables a series of measurement methods and plausibility tests to assess the daphnia behaviour using different criteria. Speed measurements: D average speed D speed distribution Behaviour observation: D swimming height D fractal dimension - measurements for turns and circling movements, curviness Growth observation: D determination of daphnia size Location: D no. of daphnia C h a m b e r e x c h a n g e D distribution in chamber D a p h n i a T o x i m e t e r I I b b e m o l d a e n k e © Get to know your water quality using sensitive real-time biomonitoring Toxicity Index Software The concept of the toxicity index is based on the evaluation of The bbe alarm software is used to record and analyse certain measurands, such as swimming speed or height, and the data. The most important features are changes in these measurands. Only if more than two of the measurands simultaneously show unusual results within a fixed D determination of different D online display period of time does the Daphnia Toximeter II trigger an alarm. behavioural patterns D drift correction D alarm analysis D parameterisation of Due to a dynamic alarm threshold an increase in daphnia size D saving of data the measurements (growth) does not affect the alarm. and parameters D data export to Excel D graphic display of all or text files Sensitivity to toxins measurement values D print function Daphnia Toximeter Substance EC50 in μg/L alarm at μg/L Aldrin 28 27 Carbaryl 19 22 Chlorpyrifos 344 15 Cyclosarin (GF) 60 10 Cypermethrin 1.2 1 Dichlorvos 170 0.5 Dimethoat 1900 2100 Endosulfan 200 - 900 100 Lindane 800 - 6500 30 Malathion 54 10 L i v e p i c t u r e a n d t r a c e i m a g e s c r e e n Parathionethyl 8.5 10 Sarin 10 6.4 Tabun 30 36 Terbuthylazin 3400 250 Trichlorfon 80 2 Test substances in static tests and in the bbe Daphnia Toximeter Operation Sample water (0.5-2 l/h) continuously runs through the measuring chamber containing the daphnia. The live images obtained using a CCD-camera are evaluated online with an integrated PC in order to analyse changes in behaviour. If the change is statistically significant, an alarm is triggered. Depending on the physical properties of the water flow, the incoming water maybe subject to sample preparation. The integrated preparation unit filters the sample water, mesh 2 - 40 μm, and treats the sample with ultrasound. Particles from the filter are suspended and S h o r t r e s p o n s e t i m e t o t o x i n i m p a c t removed by this procedure. An excess of waterflow washes away the suspended matter and keeps the water preparation clean. Additionally the water is heated with a heating cartridge to an appropriate temperature to remove dissolved air. Remote access software enables direct control of the Daphnia Toximeter II via remote PC and telephone lines. Readout, opera-tional check and parameter adjustment can be performed by the user or by bbe as part of our support service. D a p h n i a T o x i m e t e r I I b b e m o l d a e n k e © Applications D drinking water supply D dam monitoring D waterway analysis and assessment D general environmental monitoring D intake assessment D toxicological evaluation D limnological work D research and education D a p h n i a T o x i m e t e r I I w i t h d r i p p a n , p u m p h e a d s a n d f i l t e r u n i t Features Specifications D 1- or 2-chamber systems with up to 10 daphnia each D a p h n i a T o x i m e t e r D sample preparation: Measurement procedure video image analysis ultrasonic filter unit D automatic daphnia feeding with Housing material varnished steel plate ready prepared algae food D compact housing: Weight 60 kg IP54 security protection class Dimensions (H x W x D) 880 x 800 x 500 mm D separate compartment for flow-through cell and electrical Protection class IP54 components Mains supply 230/110 V 60/50 Hz Power consumption 600 W Sample temperature 0 - 30 °C Water requirement 200 l / h D modern touchscreen PC, 15” monitor, Windows XP, Maintenance interval ≥ 7 days flexible USB keyboard Sample inflow free inflow/tube pump D alarm evaluation with bbe alarm database software Hardware touchscreen PC, 15" monitor tion! D hardware diagnostic rae alt D remote control via software o modem, LAN, analog output 4-20 mA (2 X), (optional) Outputs relay output (2 X), RS232, USB Subject t Your local bbe dealer… biological • biophysical • engineering bbe moldaenke Preetzer Chaussee 177 | 24222 Schwewntinetal | Germany phone (+49) 431 38040 -0 fax (+49) 431 38040 -10 e-mail info@bbe-moldaenke.de net www.bbe-moldaenke.de