Chlorolab 3+ Liquid-phase oxygen electrode system for advanced photosynthesis & respiration studies Chlorolab 3+ Liquid-phase oxygen electrode system for advanced photosynthesis & respiration studies > PC operated USB Oxylab+ electrode control unit > DW3 advanced large volume electrode chamber with 2 optical ports and integral S1 oxygen electrode disc > LH36/2R LED light source (up to 900 µmols m-2 s-1) with automated control via user-defined PFD light tables > Suitable for liquid-phase samples between 1 - 20ml (min. 15ml if illuminated) with 0 – 100% oxygen concentration > 24 bit high resolution measurement of oxygen signals > Integral systems for measurement of pH & other ion-selective electrode (ISE) signals with 16 bit resolution > Onboard LCD readings of oxygen, auxiliary & ISE signals > 2 channel capability via purchase of additional systems > Quantitherm PAR/Temp sensor for light source calibration > OxyTrace+ Windows® software for data acquisition, hardware control & data analysis > Real time 0 – 4.5v analogue output of oxygen signal Oxylab+ electrode control unit The next generation Oxylab+ oxygen electrode control unit combines striking aesthetics with enhanced features and functionality offering significant advances in flexibility and performance over previous generations of electrode control unit. As part of a complete system, Oxylab+ provides a convenient yet powerful tool for measurements of oxygen evolution or uptake across a broad range of liquid-phase samples from chloroplast extractions to mitochondrial suspensions with oxygen concentrations up to 100%. Oxylab+ offers unrivalled price vs. performance combining simplicity of operation with an enviable feature set. The outstanding flexibility ensures Oxylab+ is equally useful in both a teaching and research capacity. 24 bit resolution allows detection of minute changes in oxygen tension without needing to apply instrument gain. This results in beautiful, noise free traces even when zoomed close in on areas of interest. Integral electronics provide control over an LED light source with automatic intensity changes handled by user-defined PFD light tables in software. The system allows realtime graphing of signals from auxiliary inputs and ion-selective electrodes providing scope for comprehensive analysis of oxygen activity simultaneously with signals such as pH, TPP+, calcium, potassium and hydrogen ions. Signals from all inputs are additionally displayed on an LCD screen mounted within the front panel of the Oxylab+ control unit. Up to 2 individual Oxylab+ control units may be linked to a single PC and operated simultaneously from OxyTrace+ software providing a powerful, multi-channel system. Oxygen electrode disc Since its original design in the early 1970’s by Tom Delieu and David Walker, the S1 Clark Type Oxygen Electrode disc remains largely unchanged – a true testament to the quality and reliability of the sensor. The S1 consists of a platinum cathode and silver anode set into an epoxy resin disc and is prepared for use by trapping a layer of 50% saturated KCl solution beneath an oxygen permeable PTFE membrane. A paper O spacer placed beneath the membrane acts as a wick to 2 provide a uniform layer of electrolyte between anode Membrane and cathode. Cathode (Pt) - - - - - - O + 2H O + 2e- H O + 2OH- 2 2 2 2 When a small voltage is applied across these electrodes H O + 2e- 2OH-2 2 (with the platinum negative with respect to the silver), the current which flows is at first negligible KCl bridge and the platinum becomes polarised (i.e. it adopts the externally applied potential). As this potential is increased to 700 mV, oxygen is reduced at the platinum surface, initially to hydrogen peroxide H O so that the 2 2 polarity tends to discharge as electrons are donated to oxygen (which acts as an electron acceptor). The current which then flows is stoichiometrically related to KCl electrolyte the oxygen consumed at the cathode providing a fast, in disc well effective method of detecting small changes in oxygen + + + + + + Anode (Ag) - + 4e- tension in a liquid-phase sample. 4Ag 4Ag+ + 4e- 0.6 - 0.7v 4Ag+ + 4Cl- 4AgCl DW3 electrode chamber The DW3 large volume electrode chamber is particularly suited to oxygen evolution/uptake measurements of macroalgae in seawater samples of between 1 – 20ml (15-20ml if illumination is required). The square section borosilicate glass reaction vessel with a prepared S1 electrode disc forming the floor of the vessel. A large quartz front optical port allows a large sample surface to be uniformly illuminated using the LH36/2R red LED light housing. Samples may either be in stirred suspension or in the case of laminar material, may be vertically supported and retained by the plunger such that they may be fully illuminated. Precision temperature control of sample and sensor is delivered via a concentric water jacket with self-sealing ports for connection to a thermoregulated circulating water bath. The water jacket is constructed from black acetal which provides the ability for dark adaptation of samples or oxygen measurement in complete darkness. An additional optical port on the reverse of the chamber allows other items such as additional light sources, fibre optic light guides and detectors to be mounted on the DW3 enabling spectroscopic measurements to be made. DW3 is fitted with a plunger with a precision central bore. The height of the plunger may be adjusted easily to suit liquid-phase sample volumes of between 1 - 20ml whilst the central bore easily accommodates Hamilton type syringes allowing additions/subtractions to/from the reaction vessel during an experiment. LH36/2R light source The LH36/2R light housing is designed specifically for use with the DW3 chamber. The light housing mounts directly on to the larger optical port of the DW3 and is held securely in place. The large area LED array consists of 36 red LED’s arranged in such a way to provide a high uniformity of illumination of laminar samples suspended in the square section chamber of the DW3. LH36/2R connects directly to the rear of the Oxylab+ electrode control unit. Light intensity adjustments are made automatically based on user-defined PFD tables within OxyTrace+ software. PFD tables consist of up to 20 individual steps allowing complex light response assays to be configured for automatic execution during a measurement. The LH36/2R has an integral cooling fan which automatically switches on to cool the housing when the light intensity tion reaches a certain point. This provides stability control of the light intensity when required at higher light intensity steps. tribu Optical feedback controls within the light housing serves to dis enhance the stability of the LH36/2R performance across the wer entire range of intensity. poal The LH36/2R has a peak wavelength centred at 660nm with a Spectr maximum intensity of 900 µmol m-2 s-1 in DW3. 600 650 700 Wavelength (nm) Quantitherm PAR/temperature sensor The QRT1 consists of a handheld display unit combined with the QTP1+ probe sensor. For use as a calibration tool for the LH36/2R light source, the QTP1+ probe connects directly to the rear of the Oxylab+ control unit allowing a 10 step multi-intensity calibration routine within the OxyTrace+ software to be performed. QRT1 may also be operated as a standalone sensor across the range of normal measuring temperatures used for both photosynthesis and cellular respiration studies and offers maximum accuracy in the 10°C – 40°C region. For photosynthesis measurements, the PAR quantum sensor provides a displayed resolution of 1µmol m-2 s-1 throughout the 0 to 5,000 µmol m-2 s-1 range and up to a maximum of 50,000 µmol m-2 s-1 with a displayed resolution of 10 µmol m-2 s-1. OxyTrace+ software OxyTrace+ is a multi-function Windows® program supplied with Chlorolab 3+ for system configuration, calibration, data acquisition and analysis. An automated 2 step calibration routine guides the user quickly and effectively through the system calibration process using electrode values measured from air-saturated and deoxygenated water. OxyTrace+ allows simple configuration of comprehensive PFD tables consisting of up to 20 individual light steps. Light intensity adjustments are performed automatically during the measurement. OxyTrace+ also allows calibration of the LH36/2R light source from a simple software routine. This requires the QTP1 PAR/temperature sensor to be connected to the rear of the Oxylab+ control unit and placed into the reaction vessel prior to the addition of any liquids. A tabbed interface allows a simple transition between the different data views including oxygen electrode (and if configured, auxiliary and external ion-selective electrode) real-time output, a split screen showing real-time rate of change above the oxygen signal and tabulated numerical data. Post acquisition analysis tools allow automatic calculation of oxygen rates from user-defined rate intervals. Additional analysis tools automatically calculate rates of change for defined PFD light steps with a calculation of quantum yield presented at the end of a measurement. All files are saved as Comma Separated Values (CSV) data files opening effortlessly in external data processing packages such as MS Excel®. OxyTrace+ will run on all supported Microsoft operating systems. System components Chlorolab 3+ systems are supplied with the following components: • OXYL1+: Oxylab+ electrode control unit • DW3: Oxygen electrode chamber • S1: Oxygen electrode disc and SMB-SMB connection cable • LH36/2R: LED light housing • QRT1: Quantitherm PAR/temperature sensor • A2: Membrane applicator to assist with smooth application of electrode membrane • S2/PL: Pack of 5 magnetic followers • S4: PTFE membrane (0.0125mm x 25mm x 33m) • S10: Set of replacement o-rings for DW2/2 electrode chamber • S16: Cleaning kit for the S1 electrode disc. Technical specifications Oxylab+ electrode control unit S1 oxygen electrode disc Measuring range: Oxygen: 0 - 100%, pH: 0 - 14pH, Electrode type: Clark type polarographic sensor Aux: 0 - 4.096V Electrode output: Typically 1.6µA at 21% O2 Signal inputs: S1 O electrode (SMB), pH/ISE (BNC), Residual current: Typically 0.04µA in 0% O 2 2 Aux (8 pin Mini Din), QTP1 PAR/Temp Response time: 10 - 90% typically < 5 seconds probe (6 pin Mini Din) Oxygen consumption: Typically <0.015µmol/hr-1 Resolution: Oxygen: 0.0003% (24 bit), pH: 0.0006pH (16 bit), LH36/2R light source Aux: 62.5µV/bit (16 bit) Light source: 36 red LED array Polarising voltage: 700mV Control: Via Oxylab+ & OxyTrace+ software Input sensitivity: 0 - 9000nA Wavelength: 660nm peak wavelength Magnetic stirrer: Software controlled 150 - 900rpm Cooling: Integral automatic cooling fan in % steps Intensity: max. 900 µmols m-2 s-1 Sampling rate: 0.1 - 10 readings/s Dimensions: 74 x 52mm - 270g Electronics: Microcontroller: 16 bit high performance CPU running at 32 MHz QRT1 PAR/Temperature sensor ADC: Dual, Low power, 16/24 Bit Sigma Delta Measuring range: 0- 50000μmol m-2 s-1 (+/- 5%) in 2 Display: 61 x 2 character blue LCD ranges (0 - 5000 & 0 - 50000) in 400 - Communications: USB2.0 700nm band Analogue output: 0 - 4.5V O signal Resolution: 1µmol m-2 s-1 at 0 - 5000 2 Dimensions (HWD): 250 x 125 x 65mm - 0.63 Kg 10μmol m-2 s-1 at 5001 - 50000 Power: 95 - 260V universal input mains PAR sensor: Silicon photodiode & optical filter with supply. Output 12V DC 2.5A white acetal diffuser Temperature sensor: RT curve matched bead thermistor. 0 - 50°C/32 - 122°F (0.02°C resolution) DW3 electrode chamber Signal display: Handheld display unit. 16 x 2 LCD Suitability: Liquid-phase photosynthesis/ display. 0 - 5V analogue output of respiration PAR/ temperature values Construction: Black acetal Power requirements: 4 x 1.5V AA (LR6) cells Sample chamber: Square section borosilicate glass Typically 100 hours battery life Sample volume: 1-20ml (15ml min. for illumination) Dimensions (display): 146 x 92 x 32mm - 300g Plunger: Variable height, central bore Dimensions (QTP1): 9.5 x 107mm - 50g Temperature control: Water jacket connected to circulating water bath Optical ports: Optical port (26mm dia), Quartz window (36mm dia) Dimensions: 110 x 75 x 100mm - 400g Hansatech Instruments is Our product range consists of Purchasers of Hansatech a British company that has a range of modular solutions Instruments products can be been developing high quality for the measurement of oxygen assured of ongoing support & scientific instrumentation for using Clark type polarographic prompt & efficient attention to over 40 years. Our systems sensors. We also develop enquiries at all times. Support are used widely for teaching & chlorophyll fluorescence is available both directly & from research in cellular respiration measurement systems using our global distributor network. & photosynthesis programs both continuous excitation & Customers are encouraged to in more than 100 countries pulse-modulated measurement register their instruments on our throughout the world. We have techniques with further website which allows access to gained an enviable reputation optical instrumentation for our Support Ticketing System in for quality, reliability & excellent the measurement of sample addition to instruments manuals price/performance. chlorophyll content. & software upgrades. Hansatech Instruments Ltd Narborough Road, Pentney, King’s Lynn, Norfolk PE32 1JL, UK Tel: +44 (0)1760 338877 Fax: +44 (0)1760 337303 info@hansatech-instruments.com www.hansatech-instruments.com