Oxyview 1 Oxygen electrode measurement teaching system Oxyview 1 Oxygen electrode measurement teaching system > Convenient, low cost system for teaching of photosynthesis research & cellular respiration measurements using the oxygen electrode > Clear cast acrylic DW1/AD oxygen electrode chamber with integral Clark type polarographic oxygen electrode > Oxyview electrode control unit with integral magnetic stirrer > Oxygen content of sample displayed as digital or percentage value > User-friendly configuration via a responsive 4 button control panel and a series of comprehensive menu screens > Analogue output for connection to a chart recorder or other external recording device > Minimal footprint (90 x 135mm) allowing multiple units where bench space is limited Oxyview control unit The OXYV1 Oxyview control unit has been designed as a convenient, low cost oxygen electrode control unit for teaching studies of photosynthesis and cellular respiration using the oxygen electrode measurement technique. The Oxyview control unit is fully compatible with the range of oxygen electrode chambers produced by Hansatech Instruments thus allowing a wide range of different assays to be performed in both liquid and gas-phases. The Oxyview is configured and controlled via a front mounted control panel featuring 4 touch-sensitive buttons. Configuration is achieved by navigating through a series of simple menu screens and following the displayed guidelines for each step of the setup process. These configuration steps in-clude setting of the stirrer speed (for liquid-phase measurements) and back-off and gain settings. Once configured, the Oxyview control unit provides an accurate and stable reading of the oxygen content of the sample in question. The Oxyview control unit is powered by a 12V DC wall cube which connects directly to the rear of the unit. Also at the rear is a 0 – 5V analogue output. This allows the measured values from the control unit to be logged to an external recording device such as a chart recorder or similar datalogger accepting a 0 – 5V analogue input. Oxygen electrode disc Since its original design in the early 1970’s by Tom Delieu and David Walker, the S1 Clark Type Oxygen Electrode disc remains largely unchanged – a true testament to the quality and reliability of the sensor. The S1 consists of a platinum cathode and silver anode set into an epoxy resin disc. When a small voltage is applied across these electrodes (with the platinum negative with respect to the silver), the current which flows is at first negligible and the platinum becomes polarised (i.e. it adopts the externally applied potential). As this potential is increased to 700 mV, oxygen is reduced at the platinum surface, initially to hydrogen peroxide H O so that the polarity tends to discharge as electrons are donated 2 2 to oxygen (which acts as an electron acceptor). The current which then flows is stoichiometrically related to the oxygen consumed at the cathode providing a fast, effective method of detecting small changes in oxygen tension in a liquid-phase sample. DW1/AD electrode chamber A component that has proved itself time and again over 4 decades, the DW1/AD electrode chamber offers quality and versatility in measurements of dissolved oxygen. Developed in conjunction with the great Prof. David Walker, the DW1/AD provides a highly flexible solution for a wide range of applications covering both teaching and research in plant and biomedical science. The reaction vessel of the DW1/AD is constructed from precision bore borosilicate glass tube with a prepared S1 electrode disc forming the floor of the vessel. Precision temperature control of sample and sensor is delivered via a concentric water jacket with suitable connection ports for a thermoregulated circulating water bath. DW1/AD is fitted with a gas-tight plunger with a stoppered central bore. The height of the plunger may be adjusted easily to suit liquid-phase sample volumes of between 0.2 - 2.5ml whilst the central bore easily accommodates Hamilton type syringes allowing additions/subtractions to/from the reaction vessel during an experiment. The clear acrylic construction allows high sample visibility and uniform sample illumination using external light sources where necessary. System components Oxyview 1 systems are supplied with the following components > OXYV1 Oxyview control unit > S2/P: Pack of 5 magnetic followers > DW1/AD: Electrode chamber > S3: Pack of 2 replacement borosilicate glass > S1: Oxygen electrode disc and SMB-SMB reaction vessels connection cable > S4: Reel of PTFE membrane (0.0125mm x 25mm > A2: Membrane applicator to assist with smooth x 33m) application of electrode membrane > S7A: Set of replacement o-rings for DW1/AD > A3: Top plate key and alignment jig to dismantle > S16: Cleaning kit for the S1 electrode disc. and reassemble DW1/AD for cleaning Technical specifications Oxyview 1 electrode control unit DW1/AD electrode chamber Measuring range: Oxygen: 0 - 100% Suitability: Liquid-phase respiration/ Signal inputs: Oxygen electrode (SMB) photosynthesis Resolution: 10 x 10-6 μmols/ml at 20 °C Construction: Clear cast acrylic Polarising voltage: Selectable between 0.4 - 0.9V Sample chamber: Precision bore, borosilicate glass tube (0.7V recommended default) Sample volume: 0.2 - 2.5ml Gain: Coarse: x1, x2, x5, x10, x20, x50, x100. Temperature control: Water jacket connected to Fine: 1mV steps thermoregulated circulating water bath Back off: Signal back off in 1mV steps Dimensions (DH): 65 x 105mm Analogue output: 0 - 5V electorde signal Weight: 100g Dimensions (HWD): 90 x 135 x 85mm Plunger: Variable height plunger assembly with Weight: 320g central bore for sample additions Power: 95 - 260V universal input mains supply. Output 12V DC 2.5A S1 oxygen electrode disc Electrode type: Clark type polarographic oxygen sensor Electrode output: Typically 1.6µA at 21% O2 Residual current: Typically 0.04µA in 0% O2 Response time: 10 - 90% typically < 5 seconds Oxygen consumption: Typically <0.015µmol/hr-1 Hansatech Instruments is Our product range consists of Purchasers of Hansatech a British company that has a range of modular solutions Instruments products can be been developing high quality for the measurement of oxygen assured of ongoing support & scientific instrumentation for using Clark type polarographic prompt & efficient attention to over 40 years. Our systems sensors. We also develop enquiries at all times. Support are used widely for teaching & chlorophyll fluorescence is available both directly & from research in cellular respiration measurement systems using our global distributor network. & photosynthesis programs both continuous excitation & Customers are encouraged to in more than 100 countries pulse-modulated measurement register their instruments on our throughout the world. We have techniques with further website which allows access to gained an enviable reputation optical instrumentation for our Support Ticketing System in for quality, reliability & excellent the measurement of sample addition to instruments manuals price/performance. chlorophyll content. & software upgrades. Hansatech Instruments Ltd Narborough Road, Pentney, King’s Lynn, Norfolk PE32 1JL, UK Tel: +44 (0)1760 338877 Fax: +44 (0)1760 337303 info@hansatech-instruments.com www.hansatech-instruments.com