Science Together KNAUER Column Selection Guide 2020 for HPLC/UHPLC, Prep LC, FPLC and GPC www.knauer.net ecycled paper Printed on r Sales If you want to learn more about our products and services or get a quote, the experts from our Sales team are happy to assist you with your request. Phone: +49 30 809727-0 (workdays 9-17h CET) Fax : +49 30 8015010 Email: sales@knauer.net Support Do you have questions about the installation or the operation of your instrument or software? International Support: Contact your local KNAUER partner for support: www.knauer.net/en/Support/Distributors-worldwide Support in Germany (Austria & Switzerland on case-to-case basis): Phone: +49 30 809727-111 (workdays 9-17h CET) Fax : +49 30 8015010 Email: support@knauer.net Made in Germany since 1962 Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography. The range includes systems and components for analytical HPLC / UHPLC, preparative HPLC, fast protein liquid chromatography (FPLC), multi-column chromatography / simulated moving bed (SMB), and osmometry. Independent and family owned The founder Dr. Herbert Knauer and his wife Roswitha are still active as advisers in the company to this day. The couple‘s daughter, Alexandra Knauer, has been managing director and owner of the company since 2000. Several awards for outstanding products and innovations as well as entrepreneurial excellence make KNAUER a „leading employer“. Comprehensive service from the manufacturer Our wide-ranging services complement our excellent instruments: The trainings are performed by experts and cover a variety of topics for beginners to experienced users. The high-performance customer support and repair service provide fast and spot on solutions. If you need support for your research project, we help you to develop applications, transfer and optimize methods or develop software. Overview KNAUER column portfolio Overview on KNAUER Column Portfolio Non-native conditions Native conditions Molecular weight Molecular weight < 2000 Da > 2000 Da Soluble in Soluble in Soluble in Soluble in Proteins with Charged Group separation of water organic solvent organic solvent water specific Affinity Biomolecules high and low molecular weight Biomolecules Soluble in Soluble in Soluble in Soluble Ionic Non-ionic ACN/H2O, Hexane/ THF, Toluene, in DMSO, MeOH/H2O Heptane Chloroform DMAc, DMF Tagged Anti- Anions Cations Proteins bodies Ion-exclusion HILIC Reversed Phase (RP) Normal Phase (NP) GPC Special GPC SEC Wide pore Tag-Affinity Antibody Affinity Anion Ex- Cation Ex- Desalting/Buffer Reversed Chromatography (AC) Chromatography (AC) change (IEX) change (IEX) Exchange (SEC) Phase (RP) H-form Silica Classical C18 Silica Porous Special Hydro- Wide pore GST-tag Protein A Weak anion ex- Weak cation Desalting (USP L17) (USP L3) (USP L1) (USP L3) styrene- GPC philic classical changer exchanger divinyl- polymer polymer C18 His-tag Protein G Ca-form NH2 Hydrophilic/ NH2 benzene material material (USP L1) Strong anion Strong cation (USP L19) (USP L8) aqueous (USP L8) exchanger exchanger C18 (USP L1) Wide pore Pb-form Zwitter-ionic Diol C18A (USP L34) HILIC Hydrophobic/ (USP L20) hydrophilic/ (USP -) pH stable aqueous Na-form C18 (USP L1) Cyano (USP L1) (USP -) (USP L10) Classical Wide pore KNAUER C8 (USP L7) C8 Sepapure® (USP L7) Hydrophilic/ aqueous Wide pore C8 (USP L7) C4 (USP L26) Hydrophobic/ pH stable C8 (USP L7) C4 (USP L26) Finding the best fitting column for your HPLC/UHPLC, GPC or FPLC More details about KNAUER CN (USP L10) application always starts with looking closely at the substances you want columns and phases can be Phenyl (USP L11) to analyse or purify. found in the Column Product Selection Guide and online: This flow chart gives you a guideline how to select the right column for www.knauer.net/columns Eurokat KNAUER Applichrom® Applichrom® Applichrom® KNAUER Eurospher ABOA ABOA ABOA Eurosil your application. Start at the top and follow the decision lines all the way Eurospher II StyDiViBe DMAc-Phil, Super-OH Bioselect down to find a column recommendation. DMSO-Phil Overview KNAUER column portfolio Overview on KNAUER Column Portfolio Non-native conditions Native conditions Molecular weight Molecular weight < 2000 Da > 2000 Da Soluble in Soluble in Soluble in Soluble in Proteins with Charged Group separation of water organic solvent organic solvent water specific Affinity Biomolecules high and low molecular weight Biomolecules Soluble in Soluble in Soluble in Soluble Ionic Non-ionic ACN/H2O, Hexane/ THF, Toluene, in DMSO, MeOH/H2O Heptane Chloroform DMAc, DMF Tagged Anti- Anions Cations Proteins bodies Ion-exclusion HILIC Reversed Phase (RP) Normal Phase (NP) GPC Special GPC SEC Wide pore Tag-Affinity Antibody Affinity Anion Ex- Cation Ex- Desalting/Buffer Reversed Chromatography (AC) Chromatography (AC) change (IEX) change (IEX) Exchange (SEC) Phase (RP) H-form Silica Classical C18 Silica Porous Special Hydro- Wide pore GST-tag Protein A Weak anion ex- Weak cation Desalting (USP L17) (USP L3) (USP L1) (USP L3) styrene- GPC philic classical changer exchanger divinyl- polymer polymer C18 His-tag Protein G Ca-form NH2 Hydrophilic/ NH2 benzene material material (USP L1) Strong anion Strong cation (USP L19) (USP L8) aqueous (USP L8) exchanger exchanger C18 (USP L1) Wide pore Pb-form Zwitter-ionic Diol C18A (USP L34) HILIC Hydrophobic/ (USP L20) hydrophilic/ (USP -) pH stable aqueous Na-form C18 (USP L1) Cyano (USP L1) (USP -) (USP L10) Classical Wide pore KNAUER C8 (USP L7) C8 Sepapure® (USP L7) Hydrophilic/ aqueous Wide pore C8 (USP L7) C4 (USP L26) Hydrophobic/ pH stable C8 (USP L7) C4 (USP L26) Finding the best fitting column for your HPLC/UHPLC, GPC or FPLC More details about KNAUER CN (USP L10) application always starts with looking closely at the substances you want columns and phases can be Phenyl (USP L11) to analyse or purify. found in the Column Product Selection Guide and online: This flow chart gives you a guideline how to select the right column for www.knauer.net/columns Eurokat KNAUER Applichrom® Applichrom® Applichrom® KNAUER Eurospher ABOA ABOA ABOA Eurosil your application. Start at the top and follow the decision lines all the way Eurospher II StyDiViBe DMAc-Phil, Super-OH Bioselect down to find a column recommendation. DMSO-Phil Eurospher II . . . . . . . . . . . . . . . . . . . . . . . . .1 AppliChrom® . . . . . . . . . . . . . . . . . . . . . . .41 Eurospher II 100 C18 - USP L1. . . . . . . . . . . . . . . . . 2 AppliChrom® ABOA SuperOH-P . . . . . . . . . . . . .42 Eurospher II 100 C18 A - USP L1. . . . . . . . . . . . . . . 4 AppliChrom® ABOA StyDiViBe-P . . . . . . . . . . . .43 Eurospher II 100 C18 H - USP L1. . . . . . . . . . . . . . . 6 AppliChrom® ABOA DMSO-PHIL-P. . . . . . . . . . .44 Eurospher II 100 C18 P - USP L1 . . . . . . . . . . . . . . . 8 AppliChrom® ABOA DMAC-Phil-P . . . . . . . . . . .45 Eurospher II 100 C8 - USP L7 . . . . . . . . . . . . . . . . 10 AppliChrom® ABOA CatPhil-P . . . . . . . . . . . . . . .46 Eurospher II 100 C8 A - USP L7. . . . . . . . . . . . . . . 12 Eurospher II 100 Phenyl - USP L11 . . . . . . . . . . . . 14 Sepapure® . . . . . . . . . . . . . . . . . . . . . . . . .47 Eurospher II 100 NH2 - USP L8 . . . . . . . . . . . . . . . 16 Antibody Affinity Chromatography . . . . . . . . . . .48 Eurospher II 100 Si . . . . . . . . . . . . . . . . . . . . . . . . . 18 Tag-Affinity Chromatography. . . . . . . . . . . . . . . . 49 Eurospher II 100 Diol . . . . . . . . . . . . . . . . . . . . . . .20 Ion-Exchange Chromatography. . . . . . . . . . . . . .50 Most common HPLC modes . . . . . . . . . . . . . . . . .20 Size Exclusion Chromatography . . . . . . . . . . . . . 51 Eurospher II 100 CN . . . . . . . . . . . . . . . . . . . . . . .22 Desalting Columns . . . . . . . . . . . . . . . . . . . . . . . . . 51 Eurospher II 100 HILIC . . . . . . . . . . . . . . . . . . . . . . 24 Columns for SMB processes. . . . . . . . . 52 Eurospher II Chiral . . . . . . . . . . . . . . . . . 25 Chiral selectors . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Column kits . . . . . . . . . . . . . . . . . . . . . . . 54 Characteristics of chiral separations . . . . . . . . . .25 Eurospher II RP Analytical Column Kit . . . . . . . .54 Eurospher II Chiral AM . . . . . . . . . . . . . . . . . . . . .26 Sepapure® Column Kits. . . . . . . . . . . . . . . . . . . . .54 Eurospher II Chiral OM . . . . . . . . . . . . . . . . . . . . .28 Eurospher II Chiral NR . . . . . . . . . . . . . . . . . . . . . .30 Column care and use . . . . . . . . . . . . . . . 55 Eurosil Bioselect . . . . . . . . . . . . . . . . . . . 32 Equilibration and storage of silica gel based columns like Eurospher II . . . . . . . . . . . . . . . . . . .55 Eurosil Bioselect 300 C18 - USP L1 . . . . . . . . . . .33 Regeneration of silica gel based columns like Eurosil Bioselect 300 C18A - USP L1 . . . . . . . . . .34 Eurospher II . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Eurosil Bioselect 300 C8 - USP L7 . . . . . . . . . . . .35 Eurokat column care and regeneration . . . . . . .56 Eurosil Bioselect 300 C4 - USP L26 . . . . . . . . . . .36 KNAUER brochures . . . . . . . . . . . . . . . . 58 Eurokat . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 Eurokat H - USP L17. . . . . . . . . . . . . . . . . . . . . . . . .38 Conversion tables. . . . . . . . . . . . . . . . . . 59 Eurokat Pb & Ca - USP L34 / USP L19. . . . . . . . . .39 Eurokat Na . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40 Notes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 Terms and conditions. . . . . . . . . . . . . . . .61 Eurospher II Eurospher II offers outstanding mechanical and chemical stability. With physical properties very similar to those of Kromasil 100, Eurospher II columns can be used to replace Kromasil® columns, providing excellent peak symmetry for acids, bases, and neutrals. Compared to Kromasil® 100, Eurospher II has • nearly the same particle shape • a lower metal impurity specification • higher mechanical stability Available column dimensions • comparable selectivity in RP mode aaaaaaaaaaaa (Eurospher II C18 H vs. Kromasil® 100 C18) Physical properties Silica gel ultra pure, > 99.99 % Metal content < 10 ppm Particle size 2 / 3 / 5 / 10 μm (15 / 20–45 μm upon request) Particle form spherical Pore size 100 Å Specific surface 320 ± 20 m2/g Pore volume 0.8 ml/g Density 430 g/l Recommended application areas KNAUER Column Selection Guide | 1 Eurospher II • Ultra pure, spherical high performance HPLC phase based on silica gel • Unpolar, monomeric C18 (Octadecyl) modification, endcapped, with 16 % carbon content (~ 50 % endcapping). Properties Separation mechanism: Hydrophobic interaction Key features Recommended application areas High-class HPLC phase perfectly suited to take on routine analyses as well as the most ambitious • acidic, basic and neutral analytes in reversed phase chromatography tasks, classical C18 phase with ca. mode, for example sulphonamides 50 % endcapping and resulting 16 % carbon content, • anabolic steroids outstanding mechanical and chemical stability. • anti-psychotics • beta blocker Tip: Never use classical C18 phases with 100 % aqueous mobile phase. The hydro- • sudan dyes phobic C18 chains will collapse. • phenols and preservatives Eurospher II 100-5 C18, 150 x 2.0 mm ID Eurospher II 100-2 C18, 50 x 2 mm ID Article number: 15BE181E2J Article number: 05BE181E2F Selectivity test mix Steroids 1. Uracil 6. Benzoic acid ethylester 1. Cortisone 4. Deoxycorticosterone 2. Aniline 7. Toluene 2. Corticosterone 5. Norgestrel 3. Phenol 8. Ethylbenenzene 3. Testosterone 6. Progesterone 4. p-Ethylaniline 5. N,N-Dimethylaniline 2 | KNAUER Column Selection Guide Eurospher II Ordering information Eurospher II 100 C18 Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...E181E2G ...E181E2J No. ...E181E2G ...E181E2J No. ...E181E2G ...E181E2J 250 mm 25C... 25CE181E2G 25CE181E2J 25D... 25DE181E2G 25DE181E2J 25E... 25EE181E2G 25EE181E2J with integrated 25X... 25XE181E2G 25XE181E2J 25W... 25WE181E2G 25WE181E2J 25V... 25VE181E2G 25VE181E2J precolumn 150 mm 15C... 15CE181E2G 15CE181E2J 15D... 15DE181E2G 15DE181E2J 15E... 15EE181E2G 15EE181E2J with integrated 15X... 15XE181E2G 15XE181E2J 15W... 15WE181E2G 15WE181E2J 15V... 15VE181E2G 15VE181E2J precolumn 100 mm 10C... 10CE181E2G 10CE181E2J 10D... 10DE181E2G 10DE181E2J 10E... 10EE181E2G 10EE181E2J with integrated 10X... 10XE181E2G 10XE181E2J 10W... 10WE181E2G 10WE181E2J 10V... 10VE181E2G 10VE181E2J precolumn 50 mm 05C... 05CE181E2G 05CE181E2J 05D... 05DE181E2G 05DE181E2J 05E... 05EE181E2G 05EE181E2J 5 mm precolumn P6C... P6CE181E2G P6CE181E2J P6D... P6DE181E2G P6DE181E2J P6E... P6EE181E2G P6EE181E2J cartridge Column length 2 mm ID Order 2 µm 3 µm No. ...E181E2F ...E181E2G 150 mm 15B... 15BE181E2F 15BE181E2G 100 mm 10B... 10BE181E2F 10BE181E2G 50 mm 05B... 05BE181E2F 05BE181E2G Eurospher II 100 C18 semi-preparative standard columns Column 8 mm ID 16 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm No. ...E181E2J ...E181E2N No. ...E181E2J ...E181E2N 250 mm 25G… 25GE181E2J 25GE181E2N 25I… 25IE181E2J 25IE181E2N 150 mm - - - 15I… 15IE181E2J 15IE181E2N 125 mm 12G... 12GE181E2J 12GE181E2N - - - 50 mm 05G… 05GE181E2J 05GE181E2N 05I… 05IE181E2J 05IE181E2N 30 mm 03G… 03GE181E2J 03GE181E2N - - - Eurospher II 100 C18 preparative standard columns Column 20 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E181E2J ...E181E2N No. ...E181E2J ...E181E2N No. ...E181E2N ...E181E2Q 250 mm 25J… 25JE181E2J 25JE181E2N 25M… 25ME181E2J 25ME181E2N 25O… 25OE181E2N 25OE181E2Q 150 mm 15J… 15JE181E2J 15JE181E2N 15M… 15ME181E2J 15ME181E2N 15O… 15OE181E2N 15OE181E2Q 50 mm 05J… 05JE181E2J 05JE181E2N 05M… 05ME181E2J 05ME181E2N 05O… 05OE181E2N 05OE181E2Q Eurospher II 100 C18 preparative dAX columns with dynamic axial compression Column 25 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E181E2J ...E181E2N No. ...E181E2J ...E181E2N No. ...E181E2N ...E181E2Q 250 mm 25T… 25TE181E2J 25TE181E2N 25U… 25UE181E2J 25UE181E2N 25Z… 25ZE181E2N 25ZE181E2Q 150 mm 15T… 15TE181E2J 15TE181E2N 15U… 15UE181E2J 15UE181E2N 15Z… 15ZE181E2N 15ZE181E2Q 50 mm 05T… 05TE181E2J 05TE181E2N 05U… 05UE181E2J 05UE181E2N 05Z… 05ZE181E2N 05ZE181E2Q KNAUER Column Selection Guide | 3 Eurospher II • Ultra pure, spherical high performance HPLC phase based on silica gel • Polar, monomeric C18 (Octadecyl) modification, polar endcapping, with 10 % carbon content (~ 50 % endcapping) Properties Separation mechanism: Hydrophobic and polar interaction very polar compounds, basic pharmaceutical ingredients, water soluble vitamins, catecholamines Key features as well as organic acids. C18 A phase with alternative, more polar selectivity, suited for the use with pure aqueous mobile phases, Tip: With an endcapping the influence of non-50 % endcapping and resulting 10 % carbon content, derivatized silanol groups can be minimized. outstanding mechanical and chemical stability, suited The endcapping reagent is generally a smaller for analytical as well as semi preparative and silane than used for derivatisation. This preparative applications. treatment reduces the unwanted interaction of polar or charged analytes (acids, bases ect.) Recommended application areas because the amount of available silanol groups is reduced. Polar endcapped C18 phase for water soluble and polar analytes, 100 % aqueous eluents for analysis of Eurospher II 100-3 C18 A, 100 x 3 mm ID Eurospher II 100-2 C18 A, 100 x 2 mm ID Article number: 10CE184E2G Article number: 10BE184E2F Aldehydes/Ketones Water soluble vitamins DNPH Derivates of: 1. Ascorbic acid 5. Nicotinamid 2. Nicotinic acid 6. Folic acid 1. Formaldehyde 4. Acroleine 3. Thiamine 7. Cyanocobalamine 2. Acetaldehyde 5. Propionaldehyde 4. Pyridoxin 8. Riboflavin 3. Acetone 6. Crotonaldehyde 4 | KNAUER Column Selection Guide Eurospher II Ordering information Eurospher II 100 C18 A Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...E184E2G ...E184E2J No. ...E184E2G ...E184E2J No. ...E184E2G ...E184E2J 250 mm 25C... 25CE184E2G 25CE184E2J 25D... 25DE184E2G 25DE184E2J 25E... 25EE184E2G 25EE184E2J with integrated 25X... 25XE184E2G 25XE184E2J 25W... 25WE184E2G 25WE184E2J 25V... 25VE184E2G 25VE184E2J precolumn 150 mm 15C... 15CE184E2G 15CE184E2J 15D... 15DE184E2G 15DE184E2J 15E... 15EE184E2G 15EE184E2J with integrated 15X... 15XE184E2G 15XE184E2J 15W... 15WE184E2G 15WE184E2J 15V... 15VE184E2G 15VE184E2J precolumn 100 mm 10C... 10CE184E2G 10CE184E2J 10D... 10DE184E2G 10DE184E2J 10E... 10EE184E2G 10EE184E2J with integrated 10X... 10XE184E2G 10XE184E2J 10W... 10WE184E2G 10WE184E2J 10V... 10VE184E2G 10VE184E2J precolumn 50 mm 05C... 05CE184E2G 05CE184E2J 05D... 05DE184E2G 05DE184E2J 05E... 05EE184E2G 05EE184E2J 5 mm precolumn P6C... P6CE184E2G P6CE184E2J P6C... P6CE184E2G P6CE184E2J P6E... P6EE184E2G P6EE184E2J cartridge Column length 2 mm ID Order 2 µm 3 µm No. ...E184E2F ...E184E2G 150 mm 15B... 15BE184E2F 15BE184E2G 100 mm 10B... 10BE184E2F 10BE184E2G 50 mm 05B... 05BE184E2F 05BE184E2G Eurospher II 100 C18 A semi-preparative standard columns Column 8 mm ID 16 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm No. ...E184E2J ...E184E2N No. ...E184E2J ...E184E2N 250 mm 25G… 25GE184E2J 25GE184E2N 25I… 25IE184E2J 25IE184E2N 150 mm - - - 15I… 15IE184E2J 15IE184E2N 125 mm 12G... 12GE184E2J 12GE184E2N - - - 50 mm 05G… 05GE184E2J 05GE184E2N 05I… 05IE184E2J 05IE184E2N 30 mm 03G… 03GE184E2J 03GE184E2N - - - Eurospher II 100 C18 A preparative standard columns Column 20 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E184E2J ...E184E2N No. ...E184E2J ...E184E2N No. ...E184E2N ...E184E2Q 250 mm 25J… 25JE184E2J 25JE184E2N 25M… 25ME184E2J 25ME184E2N 25O… 25OE184E2N 25OE184E2Q 150 mm 15J… 15JE184E2J 15JE184E2N 15M… 15ME184E2J 15ME184E2N 15O… 15OE184E2N 15OE184E2Q 50 mm 05J… 05JE184E2J 05JE184E2N 05M… 05ME184E2J 05ME184E2N 05O… 05OE184E2N 05OE184E2Q Eurospher II 100 C18 A preparative dAX columns with dynamic axial compression Column 25 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E184E2J ...E184E2N No. ...E184E2J ...E184E2N No. ...E184E2N ...E184E2Q 250 mm 25T… 25TE184E2J 25TE184E2N 25U… 25UE184E2J 25UE184E2N 25Z… 25ZE184E2N 25ZE184E2Q 150 mm 15T… 15TE184E2J 15TE184E2N 15U… 15UE184E2J 15UE184E2N 15Z… 15ZE184E2N 15ZE184E2Q 50 mm 05T… 05TE184E2J 05TE184E2N 05U… 05UE184E2J 05UE184E2N 05Z… 05ZE184E2N 05ZE184E2Q KNAUER Column Selection Guide | 5 Eurospher II • Ultra pure, spherical high performance HPLC phase based on silica gel • Unpolar, monomeric C18 (Octadecyl) modification, high efficiency endcapping with 17 % carbon content (~ 99 % endcapping) Properties Separation mechanism: Hydrophobic interaction Key features Tip: All analytical KNAUER columns can be Unpolar C18 phase with high efficiency endcapping used in reversed flow direction for example and 17 % carbon content, therewith higher pH stability for cleaning. in the range of 1-12, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications. Recommended application areas Recommended alternative to Kromasil 100 C18, for acidic, basic and neutral analytes in reversed phase mode with extended pH range. Eurospher II 100-3 C18 H, 150 x 3.0 mm ID Eurospher II 100-2 C18 H, 50 x 2 mm ID Article number: 15CE185E2G Article number: 05BE185E2F Polyphenols Phenols 1. Gallic acid 5. Rutin 1. Phenol 7. 2-Methyl-4.6- 2. Chlorogenic acid 6. Kaempferol-3-o-rutinoside 2. 4-Nitrophenol dinitrophenol 3. Catechin 7. Myricitin 3. 2.4-Dinitrophenol 8. 4-Chloro-3-Methylphenol 4. Epicatechin 8. Quercetin 4. 2-Nitrophenol 9. 4-Dichlorophenol 5. 2-Chlorophenol 10. 6-Trichlorophenol 6. 2.3-Dimethylphenol 11. Pentachlorophenol 6 | KNAUER Column Selection Guide Eurospher II Ordering information Eurospher II 100 C18 H Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...E185E2G ...E185E2J No. ...E185E2G ...E185E2J No. ...E185E2G ...E185E2J 250 mm 25C... 25CE185E2G 25CE185E2J 25D... 25DE185E2G 25DE185E2J 25E... 25EE185E2G 25EE185E2J with integrated 25X... 25XE185E2G 25XE185E2J 25W... 25WE185E2G 25WE185E2J 25V... 25VE185E2G 25VE185E2J precolumn 150 mm 15C... 15CE185E2G 15CE185E2J 15D... 15DE185E2G 15DE185E2J 15E... 15EE185E2G 15EE185E2J with integrated 15X... 15XE185E2G 15XE185E2J 15W... 15WE185E2G 15WE185E2J 15V... 15VE185E2G 15VE185E2J precolumn 100 mm 10C... 10CE185E2G 10CE185E2J 10D... 10DE185E2G 10DE185E2J 10E... 10EE185E2G 10EE185E2J with integrated 10X... 10XE185E2G 10XE185E2J 10W... 10WE185E2G 10WE185E2J 10V... 10VE185E2G 10VE185E2J precolumn 50 mm 05C... 05CE185E2G 05CE185E2J 05D... 05DE185E2G 05DE185E2J 05E... 05EE185E2G 05EE185E2J 5 mm precolumn P6C... P6CE185E2G P6CE185E2J P6C... P6CE185E2G P6CE185E2J P6E... P6EE185E2G P6EE185E2J cartridge Column length 2 mm ID Order 2 µm 3 µm No. ...E185E2F ...E185E2G 150 mm 15B... 15BE185E2F 15BE185E2G 100 mm 10B... 10BE185E2F 10BE185E2G 50 mm 05B... 05BE185E2F 05BE185E2G Eurospher II 100 C18 H semi-preparative standard columns Column 8 mm ID 16 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm No. ...E185E2J ...E185E2N No. ...E185E2J ...E185E2N 250 mm 25G… 25GE185E2J 25GE185E2N 25I… 25IE185E2J 25IE185E2N 150 mm - - - 15I… 15IE185E2J 15IE185E2N 125 mm 12G... 12GE185E2J 12GE185E2N - - - 50 mm 05G… 05GE185E2J 05GE185E2N 05I… 05IE185E2J 05IE185E2N 30 mm 03G… 03GE185E2J 03GE185E2N - - - Eurospher II 100 C18 H preparative standard columns Column 20 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E185E2J ...E185E2N No. ...E185E2J ...E185E2N No. ...E185E2N ...E185E2Q 250 mm 25J… 25JE185E2J 25JE185E2N 25M… 25ME185E2J 25ME185E2N 25O… 25OE185E2N 25OE185E2Q 150 mm 15J… 15JE185E2J 15JE185E2N 15M… 15ME185E2J 15ME185E2N 15O… 15OE185E2N 15OE185E2Q 50 mm 05J… 05JE185E2J 05JE185E2N 05M… 05ME185E2J 05ME185E2N 05O… 05OE185E2N 05OE185E2Q Eurospher II 100 C18 H preparative dAX columns with dynamic axial compression Column 25 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E185E2J ...E185E2N No. ...E185E2J ...E185E2N No. ...E185E2N ...E185E2Q 250 mm 25T… 25TE185E2J 25TE185E2N 25U… 25UE185E2J 25UE185E2N 25Z… 25ZE185E2N 25ZE185E2Q 150 mm 15T… 15TE185E2J 15TE185E2N 15U… 15UE185E2J 15UE185E2N 15Z… 15ZE185E2N 15ZE185E2Q 50 mm 05T… 05TE185E2J 05TE185E2N 05U… 05UE185E2J 05UE185E2N 05Z… 05ZE185E2N 05ZE185E2Q KNAUER Column Selection Guide | 7 Eurospher II • Ultra pure, spherical high performance HPLC phase based on silica gel • Unpolar, trifunctional C18 (Octadecyl) modification, high efficiency endcapping, with 20 % carbon content (~ 99 % endcapping) Properties Separation mechanism: Hydrophobic and steric interaction Tip: Not all C18 phases are comparable! When replacing an existing column, always Key features have a close look at the characteristics like carbon load, pore size and specific surface KNAUERś most unpolar C18 phase, polymeric, high area. efficiency endcapping of 99 % and 20 % carbon content, higher pH stability in the range of 1-12, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications. Recommended application areas Alternative selectivity to standard C18; stationary phase in Eurospher II C18 family with the highest carbon load; fully endcapped; excellent shape selectivity and pH stability. Eurospher II 100-3 C18 P, 100 x 3.0 mm ID Article number: 10CE182E2G Sedativa Softdrink additives 1. Barbital 3. Prominal 1. Acesulfam 5. Aspartam 2. Luminal 4. Revonal 2. Saccharin 6. Benzoic acid 3. Vanilline 7. Sorbic acid 4. Caffeine 8 | KNAUER Column Selection Guide Eurospher II Ordering information Eurospher II 100 C18 P Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...E182E2G ...E182E2J No. ...E182E2G ...E182E2J No. ...E182E2G ...E182E2J 250 mm 25C... 25CE182E2G 25CE182E2J 25D... 25DE182E2G 25DE182E2J 25E... 25EE182E2G 25EE182E2J with integrated 25X... 25XE182E2G 25XE182E2J 25W... 25WE182E2G 25WE182E2J 25V... 25VE182E2G 25VE182E2J precolumn 150 mm 15C... 15CE182E2G 15CE182E2J 15D... 15DE182E2G 15DE182E2J 15E... 15EE182E2G 15EE182E2J with integrated 15X... 15XE182E2G 15XE182E2J 15W... 15WE182E2G 15WE182E2J 15V... 15VE182E2G 15VE182E2J precolumn 100 mm 10C... 10CE182E2G 10CE182E2J 10D... 10DE182E2G 10DE182E2J 10E... 10EE182E2G 10EE182E2J with integrated 10X... 10XE182E2G 10XE182E2J 10W... 10WE182E2G 10WE182E2J 10V... 10VE182E2G 10VE182E2J precolumn 50 mm 05C... 05CE182E2G 05CE182E2J 05D... 05DE182E2G 05DE182E2J 05E... 05EE182E2G 05EE182E2J 5 mm precolumn P6C... P6CE182E2G P6CE182E2J P6C... P6CE182E2G P6CE182E2J P6E... P6EE182E2G P6EE182E2J cartridge Column length 2 mm ID Order 2 µm 3 µm No. ...E182E2F ...E182E2G 150 mm 15B... 15BE182E2F 15BE182E2G 100 mm 10B... 10BE182E2F 10BE182E2G 50 mm 05B... 05BE182E2F 05BE182E2G Eurospher II 100 C18 P semi-preparative standard columns Column 8 mm ID 16 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm No. ...E182E2J ...E182E2N No. ...E182E2J ...E182E2N 250 mm 25G… 25GE182E2J 25GE182E2N 25I… 25IE182E2J 25IE182E2N 150 mm - - - 15I… 15IE182E2J 15IE182E2N 125 mm 12G... 12GE182E2J 12GE182E2N - - - 50 mm 05G… 05GE182E2J 05GE182E2N 05I… 05IE182E2J 05IE182E2N 30 mm 03G… 03GE182E2J 03GE182E2N - - - Eurospher II 100 C18 P preparative standard columns Column 20 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E182E2J ...E182E2N No. ...E182E2J ...E182E2N No. ...E182E2N ...E182E2Q 250 mm 25J… 25JE182E2J 25JE182E2N 25M… 25ME182E2J 25ME182E2N 25O… 25OE182E2N 25OE182E2Q 150 mm 15J… 15JE182E2J 15JE182E2N 15M… 15ME182E2J 15ME182E2N 15O… 15OE182E2N 15OE182E2Q 50 mm 05J… 05JE182E2J 05JE182E2N 05M… 05ME182E2J 05ME182E2N 05O… 05OE182E2N 05OE182E2Q Eurospher II 100 C18 P preparative dAX columns with dynamic axial compression Column 25 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E182E2J ...E182E2N No. ...E182E2J ...E182E2N No. ...E182E2N ...E182E2Q 250 mm 25T… 25TE182E2J 25TE182E2N 25U… 25UE182E2J 25UE182E2N 25Z… 25ZE182E2N 25ZE182E2Q 150 mm 15T… 15TE182E2J 15TE182E2N 15U… 15UE182E2J 15UE182E2N 15Z… 15ZE182E2N 15ZE182E2Q 50 mm 05T… 05TE182E2J 05TE182E2N 05U… 05UE182E2J 05UE182E2N 05Z… 05ZE182E2N 05ZE182E2Q KNAUER Column Selection Guide | 9 Eurospher II • Ultra pure, spherical high performance HPLC phase based on silica gel • Monomeric C8 (Octyl) modification, standard endcapping, with 10 % carbon content (~ 50 % endcapping) Properties Separation mechanism: Hydrophobic interaction Tip: Column lifetime can be extended by (lower compared to C18 phases) using moderate conditions like temperatures not higher than 45 °C, backpressure below Key features the maximum pressure and a good sample preparation. Classical C8 phase, standard 50 % endcapping and 10 % carbon load, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications. Recommended application areas Similar selectivity to C18 phase but less retention due to the lower hydrophobicity; useful for determination of water soluble vitamins, steroids, catecholamines, sedatives etc. Eurospher II 100-5 C8, 150 x 4.6 mm ID Eurospher 100-5 C8, 125 x 4.0 mm ID Article number: 15EE081E2J Article number: 12DE081ESJ Preservatives Ingredients of cosmetics 1. p-hydroxy benzoic acid 5. p-hydroxy methylbenzoate 1. Bisabolol oxide A 2. 2-methoxy benzoic acid 6. p-hydroxy ethylbenzoate 2. Bisabolol oxide B 3. benzoic acid 7. p-hydroxy propylbenzoate 4. sorbic acid 8. p-hydroxy butylbenzoate 10 | KNAUER Column Selection Guide Eurospher II Ordering information Eurospher II 100 C8 Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...E081E2G ...E081E2J No. ...E081E2G ...E081E2J No. ...E081E2G ...E081E2J 250 mm 25C... 25CE081E2G 25CE081E2J 25D... 25DE081E2G 25DE081E2J 25E... 25EE081E2G 25EE081E2J with integrated 25X... 25XE081E2G 25XE081E2J 25W... 25WE081E2G 25WE081E2J 25V... 25VE081E2G 25VE081E2J precolumn 150 mm 15C... 15CE081E2G 15CE081E2J 15D... 15DE081E2G 15DE081E2J 15E... 15EE081E2G 15EE081E2J with integrated 15X... 15XE081E2G 15XE081E2J 15W... 15WE081E2G 15WE081E2J 15V... 15VE081E2G 15VE081E2J precolumn 100 mm 10C... 10CE081E2G 10CE081E2J 10D... 10DE081E2G 10DE081E2J 10E... 10EE081E2G 10EE081E2J with integrated 10X... 10XE081E2G 10XE081E2J 10W... 10WE081E2G 10WE081E2J 10V... 10VE081E2G 10VE081E2J precolumn 50 mm 05C... 05CE081E2G 05CE081E2J 05D... 05DE081E2G 05DE081E2J 05E... 05EE081E2G 05EE081E2J 5 mm precolumn P6C... P6CE081E2G P6CE081E2J P6C... P6CE081E2G P6CE081E2J P6E... P6EE081E2G P6EE081E2J cartridge Column length 2 mm ID Order 2 µm 3 µm No. ...E081E2F ...E081E2G 150 mm 15B... 15BE081E2F 15BE081E2G 100 mm 10B... 10BE081E2F 10BE081E2G 50 mm 05B... 05BE081E2F 05BE081E2G Eurospher II 100 C8 semi-preparative standard columns Column 8 mm ID 16 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm No. ...E081E2J ...E081E2N No. ...E081E2J ...E081E2N 250 mm 25G… 25GE081E2J 25GE081E2N 25I… 25IE081E2J 25IE081E2N 150 mm - - - 15I… 15IE081E2J 15IE081E2N 125 mm 12G... 12GE081E2J 12GE081E2N - - - 50 mm 05G… 05GE081E2J 05GE081E2N 05I… 05IE081E2J 05IE081E2N 30 mm 03G… 03GE081E2J 03GE081E2N - - - Eurospher II 100 C8 preparative standard columns Column 20 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E081E2J ...E081E2N No. ...E081E2J ...E081E2N No. ...E081E2N ...E081E2Q 250 mm 25J… 25JE081E2J 25JE081E2N 25M… 25ME081E2J 25ME081E2N 25O… 25OE081E2N 25OE081E2Q 150 mm 15J… 15JE081E2J 15JE081E2N 15M… 15ME081E2J 15ME081E2N 15O… 15OE081E2N 15OE081E2Q 50 mm 05J… 05JE081E2J 05JE081E2N 05M… 05ME081E2J 05ME081E2N 05O… 05OE081E2N 05OE081E2Q Eurospher II 100 C8 preparative dAX columns with dynamic axial compression Column 25 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E081E2J ...E081E2N No. ...E081E2J ...E081E2N No. ...E081E2N ...E081E2Q 250 mm 25T… 25TE081E2J 25TE081E2N 25U… 25UE081E2J 25UE081E2N 25Z… 25ZE081E2N 25ZE081E2Q 150 mm 15T… 15TE081E2J 15TE081E2N 15U… 15UE081E2J 15UE081E2N 15Z… 15ZE081E2N 15ZE081E2Q 50 mm 05T… 05TE081E2J 05TE081E2N 05U… 05UE081E2J 05UE081E2N 05Z… 05ZE081E2N 05ZE081E2Q KNAUER Column Selection Guide | 11 Eurospher II • Ultra pure, spherical high performance HPLC phase based on silica gel • Monomeric C8 (Octyl) modification, standard endcapping, with 10 % carbon content (~ 50 % endcapping) Properties Separation mechanism: Hydrophobic interaction (lower compared to C18 phases) Tip: Caution! Even for short term storage all buffer solutions have to be rinsed from the Key features column to prevent crystallization effects. Classical C8 phase, standard 50 % endcapping and 10 % carbon load, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications. Recommended application areas Similar selectivity to C18 phase but less retention due to the lower hydrophobicity; useful for determination of water soluble vitamins, steroids, catecholamines, sedatives etc. Eurospher II 100-5 C8A, 100 x 3 mm ID Article number: 10CE084E2G 1. Ascorbic acid 2. Acesulfam K 3. Saccharin 4. Caffeine 5. Aspartame 6. Benzoic acid 7. Sorbic acid AUm 12 | KNAUER Column Selection Guide Eurospher II Ordering information Eurospher II 100 C8 A Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...E084E2G ...E084E2J No. ...E084E2G ...E084E2J No. ...E084E2G ...E084E2J 250 mm 25C... 25CE084E2G 25CE084E2J 25D... 25DE084E2G 25DE084E2J 25E... 25EE084E2G 25EE084E2J with integrated 25X... 25XE084E2G 25XE084E2J 25W... 25WE084E2G 25WE084E2J 25V... 25VE084E2G 25VE084E2J precolumn 150 mm 15C... 15CE084E2G 15CE084E2J 15D... 15DE084E2G 15DE084E2J 15E... 15EE084E2G 15EE084E2J with integrated 15X... 15XE084E2G 15XE084E2J 15W... 15WE084E2G 15WE084E2J 15V... 15VE084E2G 15VE084E2J precolumn 100 mm 10C... 10CE084E2G 10CE084E2J 10D... 10DE084E2G 10DE084E2J 10E... 10EE084E2G 10EE084E2J with integrated 10X... 10XE084E2G 10XE084E2J 10W... 10WE084E2G 10WE084E2J 10V... 10VE084E2G 10VE084E2J precolumn 50 mm 05C... 05CE084E2G 05CE084E2J 05D... 05DE084E2G 05DE084E2J 05E... 05EE084E2G 05EE084E2J 5 mm precolumn P6C... P6CE084E2G P6CE084E2J P6C... P6CE084E2G P6CE084E2J P6E... P6EE084E2G P6EE084E2J cartridge Column length 2 mm ID Order 2 µm 3 µm No. ...E084E2F ...E084E2G 150 mm 15B... 15BE084E2G 15BE084E2J 100 mm 10B... 10BE084E2G 10BE084E2J 50 mm 05B... 05BE084E2G 05BE084E2J Eurospher II 100 C8 A semi-preparative standard columns Column 8 mm ID 16 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm No. ...E084E2J ...E084E2N No. ...E084E2J ...E084E2N 250 mm 25G… 25GE084E2J 25GE084E2N 25I… 25IE084E2J 25IE084E2N 150 mm - - - 15I… 15IE084E2J 15IE084E2N 125 mm 12G... 12GE084E2J 12GE084E2N - - - 50 mm 05G… 05GE084E2J 05GE084E2N 05I… 05IE084E2J 05IE084E2N 30 mm 03G… 03GE084E2J 03GE084E2N - - - Eurospher II 100 C8 A preparative standard columns Column 20 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E084E2J ...E084E2N No. ...E084E2J ...E084E2N No. ...E084E2N ...E084E2Q 250 mm 25J… 25JE084E2J 25JE084E2N 25M… 25ME084E2J 25ME084E2N 25O… 25OE084E2N 25OE084E2Q 150 mm 15J… 15JE084E2J 15JE084E2N 15M… 15ME084E2J 15ME084E2N 15O… 15OE084E2N 15OE084E2Q 50 mm 05J… 05JE084E2J 05JE084E2N 05M… 05ME084E2J 05ME084E2N 05O… 05OE084E2N 05OE084E2Q Eurospher II 100 C8 A preparative dAX columns with dynamic axial compression Column 25 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E084E2J ...E084E2N No. ...E084E2J ...E084E2N No. ...E084E2N ...E084E2Q 250 mm 25T… 25TE084E2J 25TE084E2N 25U… 25UE084E2J 25UE084E2N 25Z… 25ZE084E2N 25ZE084E2Q 150 mm 15T… 15TE084E2J 15TE084E2N 15U… 15UE084E2J 15UE084E2N 15Z… 15ZE084E2N 15ZE084E2Q 50 mm 05T… 05TE084E2J 05TE084E2N 05U… 05UE084E2J 05UE084E2N 05Z… 05ZE084E2N 05ZE084E2Q KNAUER Column Selection Guide | 13 Eurospher II • Ultra pure, spherical high performance HPLC phase based on silica gel • Phenyl modification (Phenylpropyl), 12 % carbon content Properties Separation mechanism: Pi-Pi Interactions Key features Classical phenyl phase with 12 % carbon load, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications. Tip: For UHPLC columns like Eurospher II Recommended application areas 2 μm particle size, samples and eluents should be filtered through a 0.2 μm instead of a Alternative selectivity for aromatic and mode rately 0.45 μm membrane filter. Frits at column in-pi-pi interaction polar analytes or mixtures with and outlet have smaller pores than classical varying polarity and aromaticity. HPLC columns and block more easily. Eurospher II 100-2 Phenyl, 100 x 2 mm ID Article number: 10BE050E2F Aldehydes/Ketones Biocompatible polymer building monomers DNPH-derivatives of 1. 2-Hydroxyethylmethacrylate 1. Formaldehyde 6. Acetone 2. N-Vinyl-2-pyrrolidon 2. Acetaldehyde 7. Matrix 3. Methyl methacrylate 3. Matrix 8. Butyraldehyde 4. Ethylenglycon dimethacrylate 4. Acrolein 9. Matrix 5. Styrene 5. Propionaldehyde 10. Benzaldehyde 6. Methacryloxymethyltrimethylsilane 14 | KNAUER Column Selection Guide Eurospher II Ordering information Eurospher II 100 Phenyl Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...E050E2G ...E050E2J No. ...E050E2G ...E050E2J No. ...E050E2G ...E050E2J 250 mm 25C... 25CE050E2G 25CE050E2J 25D... 25DE050E2G 25DE050E2J 25E... 25EE050E2G 25EE050E2J with integrated 25X... 25XE050E2G 25XE050E2J 25W... 25WE050E2G 25WE050E2J 25V... 25VE050E2G 25VE050E2J precolumn 150 mm 15C... 15CE050E2G 15CE050E2J 15D... 15DE050E2G 15DE050E2J 15E... 15EE050E2G 15EE050E2J with integrated 15X... 15XE050E2G 15XE050E2J 15W... 15WE050E2G 15WE050E2J 15V... 15VE050E2G 15VE050E2J precolumn 100 mm 10C... 10CE050E2G 10CE050E2J 10D... 10DE050E2G 10DE050E2J 10E... 10EE050E2G 10EE050E2J with integrated 10X... 10XE050E2G 10XE050E2J 10W... 10WE050E2G 10WE050E2J 10V... 10VE050E2G 10VE050E2J precolumn 50 mm 05C... 05CE050E2G 05CE050E2J 05D... 05DE050E2G 05DE050E2J 05E... 05EE050E2G 05EE050E2J 5 mm precolumn P6C... P6CE050E2G P6CE050E2J P6C... P6CE050E2G P6CE050E2J P6E... P6EE050E2G P6EE050E2J cartridge Column length 2 mm ID Order 2 µm 3 µm No. ...E050E2F ...E050E2G 150 mm 15B... 15BE050E2F 15BE050E2G 100 mm 10B... 10BE050E2F 10BE050E2G 50 mm 05B... 05BE050E2F 05BE050E2G Eurospher II 100 Phenyl semi-preparative standard columns Column 8 mm ID 16 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm No. ...E050E2J ...E050E2N No. ...E050E2J ...E050E2N 250 mm 25G… 25GE050E2J 25GE050E2N 25I… 25IE050E2J 25IE050E2N 150 mm - - - 15I… 15IE050E2J 15IE050E2N 125 mm 12G... 12GE050E2J 12GE050E2N - - - 50 mm 05G… 05GE050E2J 05GE050E2N 05I… 05IE050E2J 05IE050E2N 30 mm 03G… 03GE050E2J 03GE050E2N - - - Eurospher II 100 Phenyl preparative standard columns Column 20 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E050E2J ...E050E2N No. ...E050E2J ...E050E2N No. ...E050E2N ...E050E2Q 250 mm 25J… 25JE050E2J 25JE050E2N 25M… 25ME050E2J -25ME050E2N 25O… 25OE050E2N 25OE050E2Q 150 mm 15J… 15JE050E2J 15JE050E2N 15M… 15ME050E2J 15ME050E2N 15O… 15OE050E2N 15OE050E2Q 50 mm 05J… 05JE050E2J 05JE050E2N 05M… 05ME050E2J 05ME050E2N 05O… 05OE050E2N 05OE050E2Q Eurospher II 100 Phenyl preparative dAX columns with dynamic axial compression Column 25 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E050E2J ...E050E2N No. ...E050E2J ...E050E2N No. ...E050E2N ...E050E2Q 250 mm 25T… 25TE050E2J 25TE050E2N 25U… 25UE050E2J 25UE050E2N 25Z… 25ZE050E2N 25ZE050E2Q 150 mm 15T… 15TE050E2J 15TE050E2N 15U… 15UE050E2J 15UE050E2N 15Z… 15ZE050E2N 15ZE050E2Q 50 mm 05T… 05TE050E2J 05TE050E2N 05U… 05UE050E2J 05UE050E2N 05Z… 05ZE050E2N 05ZE050E2Q KNAUER Column Selection Guide | 15 Eurospher II • Ultra pure, spherical high performance HPLC phase based on silica gel • Amino modification (Aminopropyl) • 4 % carbon content (without endcapping) Properties Separation mechanism: Hydrophilic and ionic interactions Tip: A precolumn can help to protect your analytical column. Especially when working with highly matrix afflicted samples it is highly Key features recommended to use precolumns. Multi mode column for RP, NP, HILIC and IC, Aminopropyl modification without endcapping, outstanding mechanical and chemical stability. Recommended application areas Most flexible phase in the Eurospher II family; can be used in three modes: normal phase, reversed phase and ion chromatography mode (weak anion exchanger); different selectivity to the silica packing; in reversed phase mode mainly used for analysis of carbohydrates. Eurospher II 100-3 NH2, 100 x 3.0 mm ID Article number: 10CE190E2G Carbohydrates Sweeteners 1. Fructose 4. Maltose 1. Xylitol 3. Isomalt 2. Glucose 5. Maltotriose 2. Sorbitol 4. Lactitol 3. Saccharose 16 | KNAUER Column Selection Guide Eurospher II Ordering information Eurospher II 100 NH2 Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...E190E2G ...E190E2J No. ...E190E2G ...E190E2J No. ...E190E2G ...E190E2J 250 mm 25C... 25CE190E2G 25CE190E2J 25D... 25DE190E2G 25DE190E2J 25E... 25EE190E2G 25EE190E2J with integrated 25X... 25XE190E2G 25XE190E2J 25W... 25WE190E2G 25WE190E2J 25V... 25VE190E2G 25VE190E2J precolumn 150 mm 15C... 15CE190E2G 15CE190E2J 15D... 15DE190E2G 15DE190E2J 15E... 15EE190E2G 15EE190E2J with integrated 15X... 15XE190E2G 15XE190E2J 15W... 15WE190E2G 15WE190E2J 15V... 15VE190E2G 15VE190E2J precolumn 100 mm 10C... 10CE190E2G 10CE190E2J 10D... 10DE190E2G 10DE190E2J 10E... 10EE190E2G 10EE190E2J with integrated 10X... 10XE190E2G 10XE190E2J 10W... 10WE190E2G 10WE190E2J 10V... 10VE190E2G 10VE190E2J precolumn 50 mm 05C... 05CE190E2G 05CE190E2J 05D... 05DE190E2G 05DE190E2J 05E... 05EE190E2G 05EE190E2J 5 mm precolumn P6C... P6CE190E2G P6CE190E2J P6C... P6CE190E2G P6CE190E2J P6E... P6EE190E2G P6EE190E2J cartridge Eurospher II 100 NH2 semi-preparative standard columns Column 8 mm ID 16 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm No. ...E190E2J ...E190E2N No. ...E190E2J ...E190E2N 250 mm 25G… 25GE190E2J 25GE190E2N 25I… 25IE190E2J 25IE190E2N 150 mm - - - 15I… 15IE190E2J 15IE190E2N 125 mm 12G... 12GE190E2J 12GE190E2N - - - 50 mm 05G… 05GE190E2J 05GE190E2N 05I… 05IE190E2J 05IE190E2N 30 mm 03G… 03GE190E2J 03GE190E2N - - - Eurospher II 100 NH2 preparative standard columns Column 20 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E190E2J ...E190E2N No. ...E190E2J ...E190E2N No. ...E190E2N ...E190E2Q 250 mm 25J… 25JE190E2J 25JE190E2N 25M… 25ME190E2J 25ME190E2N 25O… 25OE190E2N 25OE190E2Q 150 mm 15J… 15JE190E2J 15JE190E2N 15M… 15ME190E2J 15ME190E2N 15O… 15OE190E2N 15OE190E2Q 50 mm 05J… 05JE190E2J 05JE190E2N 05M… 05ME190E2J 05ME190E2N 05O… 05OE190E2N 05OE190E2Q Eurospher II 100 NH2 preparative dAX columns with dynamic axial compression Column 25 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E190E2J ...E190E2N No. ...E190E2J ...E190E2N No. ...E190E2N ...E190E2Q 250 mm 25T… 25TE190E2J 25TE190E2N 25U… 25UE190E2J 25UE190E2N 25Z… 25ZE190E2N 25ZE190E2Q 150 mm 15T… 15TE190E2J 15TE190E2N 15U… 15UE190E2J 15UE190E2N 15Z… 15ZE190E2N 15ZE190E2Q 50 mm 05T… 05TE190E2J 05TE190E2N 05U… 05UE190E2J 05UE190E2N 05Z… 05ZE190E2N 05ZE190E2Q KNAUER Column Selection Guide | 17 Eurospher II • Ultra pure, spherical high performance HPLC phase based on silica gel • No modification, no endcapping, with 0 % carbon content Properties Hydrophilic interaction Tip: Normal phase (NP) stationary phases Key features have been the first phases for chromatography and that is why their properties of polarity High-class HPLC phase perfectly suited to take on were determined as ’ normal’ . The stationary routine analyses as well as the most ambitious phase of a NP column has polar properties chromatography tasks, outstanding mechanical and and is commonly used with nonpolar solvents chemical stability. as hexane or heptane. The other way round a reversed phase (RP) column has covalent Recommended application areas charcteristics and is used with more polar eluents, like mixtures of acetonitrile and water. Wide range of different applications, i.e. SEC (size exclusion chromatography) but also for normal phase HPLC and HILIC; good choice for analytical and preparative purposes to separate polar compounds. Eurospher 100-5 Si, Column 150 x 4 mm Article No.: 15DE000ESJ 1. 2-Nitroaniline 1. alpha-Tocopherol 2. 3-Nitroaniline 2. beta-Tocopherol 3. 4-Nitroaniline 3. gamma-Tocopherol 4. delta-Tocopherol 5. Vitamin D2 6. trans-Retinol 18 | KNAUER Column Selection Guide Eurospher II Ordering information Eurospher II 100 Si Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...E000E2G ...E000E2J No. ...E000E2G ...E000E2J No. ...E000E2G ...E000E2J 250 mm 25C... 25CE000E2G 25CE000E2J 25D... 25DE000E2G 25DE000E2J 25E... 25EE000E2G 25EE000E2J with integrated 25X... 25XE000E2G 25XE000E2J 25W... 25WE000E2G 25WE000E2J 25V... 25VE000E2G 25VE000E2J precolumn 150 mm 15C... 15CE000E2G 15CE000E2J 15D... 15DE000E2G 15DE000E2J 15E... 15EE000E2G 15EE000E2J with integrated 15X... 15XE000E2G 15XE000E2J 15W... 15WE000E2G 15WE000E2J 15V... 15VE000E2G 15VE000E2J precolumn 100 mm 10C... 10CE000E2G 10CE000E2J 10D... 10DE000E2G 10DE000E2J 10E... 10EE000E2G 10EE000E2J with integrated 10X... 10XE000E2G 10XE000E2J 10W... 10WE000E2G 10WE000E2J 10V... 10VE000E2G 10VE000E2J precolumn 50 mm 05C... 05CE000E2G 05CE000E2J 05D... 05DE000E2G 05DE000E2J 05E... 05EE000E2G 05EE000E2J 5 mm precolumn P6C... P6CE000E2G P6CE000E2J P6C... P6CE000E2G P6CE000E2J P6E... P6EE000E2G P6EE000E2J cartridge Eurospher II 100 Si semi-preparative standard columns Column 8 mm ID 16 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm No. ...E000E2J ...E000E2N No. ...E000E2J ...E000E2N 250 mm 25G… 25GE000E2J 25GE000E2N 25I… 25IE000E2J 25IE000E2N 150 mm - - - 15I… 15IE000E2J 15IE000E2N 125 mm 12G... 12GE000E2J 12GE000E2N - - - 50 mm 05G… 05GE000E2J 05GE000E2N 05I… 05IE000E2J 05IE000E2N 30 mm 03G… 03GE000E2J 03GE000E2N - - - Eurospher II 100 Si preparative standard columns Column 20 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E000E2J ...E000E2N No. ...E000E2J ...E000E2N No. ...E000E2N ...E000E2Q 250 mm 25J… 25JE000E2J 25JE000E2N 25M… 25ME000E2J 25ME000E2N 25O… 25OE000E2N 25OE000E2Q 150 mm 15J… 15JE000E2J 15JE000E2N 15M… 15ME000E2J 15ME000E2N 15O… 15OE000E2N 15OE000E2Q 50 mm 05J… 05JE000E2J 05JE000E2N 05M… 05ME000E2J 05ME000E2N 05O… 05OE000E2N 05OE000E2Q Eurospher II 100 Si preparative dAX columns with dynamic axial compression Column 25 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E000E2J ...E000E2N No. ...E000E2J ...E000E2N No. ...E000E2N ...E000E2Q 250 mm 25T… 25TE000E2J 25TE000E2N 25U… 25UE000E2J 25UE000E2N 25Z… 25ZE000E2N 25ZE000E2Q 150 mm 15T… 15TE000E2J 15TE000E2N 15U… 15UE000E2J 15UE000E2N 15Z… 15ZE000E2N 15ZE000E2Q 50 mm 05T… 05TE000E2J 05TE000E2N 05U… 05UE000E2J 05UE000E2N 05Z… 05ZE000E2N 05ZE000E2Q KNAUER Column Selection Guide | 19 Eurospher II • Ultra pure, spherical high performance HPLC phase based on silica gel • Diol, no endcapping, with 5 % carbon content Properties Hydrophilic interaction Key features High-class HPLC phase perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks, classical Diol phase without Tip: The most important column parameters endcapping and 5 % carbon content, outstanding for evaluation are retention time, peak mechanical and chemical stability. symmetry and theoretical plates. Retention time to prove the reproducibilit y of Recommended application areas measurements. Peak symmetry to estimate how good the column bed is packed (peak Alternative to the silica packing with shorter symmetry value of 1.0 is optimal). Theoretical equilibration time and comparable selectivity; due plate numbers to determine the effectiveness to the lower activity of these packings it can be also of the HPLC column. used for SEC-applications. 20 | KNAUER Column Selection Guide Eurospher II Ordering information Eurospher II 100 Diol Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...E410E2G ...E410E2J No. ...E410E2G ...E410E2J No. ...E410E2G ...E410E2J 250 mm 25C... 25CE410E2G 25CE410E2J 25D... 25DE410E2G 25DE410E2J 25E... 25EE410E2G 25EE410E2J with integrated 25X... 25XE410E2G 25XE410E2J 25W... 25WE410E2G 25WE410E2J 25V... 25VE410E2G 25VE410E2J precolumn 150 mm 15C... 15CE410E2G 15CE410E2J 15D... 15DE410E2G 15DE410E2J 15E... 15EE410E2G 15EE410E2J with integrated 15X... 15XE410E2G 15XE410E2J 15W... 15WE410E2G 15WE410E2J 15V... 15VE410E2G 15VE410E2J precolumn 100 mm 10C... 10CE410E2G 10CE410E2J 10D... 10DE410E2G 10DE410E2J 10E... 10EE410E2G 10EE410E2J with integrated 10X... 10XE410E2G 10XE410E2J 10W... 10WE410E2G 10WE410E2J 10V... 10VE410E2G 10VE410E2J precolumn 50 mm 05C... 05CE410E2G 05CE410E2J 05D... 05DE410E2G 05DE410E2J 05E... 05EE410E2G 05EE410E2J 5 mm precolumn P6C... P6CE410E2G P6CE410E2J P6C... P6CE410E2G P6CE410E2J P6E... P6EE410E2G P6EE410E2J cartridge Eurospher II 100 Diol semi-preparative standard columns Column 8 mm ID 16 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm No. ...E410E2J ...E410E2N No. ...E410E2J ...E410E2N 250 mm 25G… 25GE410E2J 25GE410E2N 25I… 25IE410E2J 25IE410E2N 150 mm - - - 15I… 15IE410E2J 15IE410E2N 125 mm 12G... 12GE410E2J 12GE410E2N - - - 50 mm 05G… 05GE410E2J 05GE410E2N 05I… 05IE410E2J 05IE410E2N 30 mm 03G… 03GE410E2J 03GE410E2N - - - Eurospher II 100 Diol preparative standard columns Column 20 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E410E2J ...E410E2N No. ...E410E2J ...E410E2N No. ...E410E2N ...E410E2Q 250 mm 25J… 25JE410E2J 25JE410E2N 25M… 25ME410E2J 25ME410E2N 25O… 25OE410E2N 25OE410E2Q 150 mm 15J… 15JE410E2J 15JE410E2N 15M… 15ME410E2J 15ME410E2N 15O… 15OE410E2N 15OE410E2Q 50 mm 05J… 05JE410E2J 05JE410E2N 05M… 05ME410E2J 05ME410E2N 05O… 05OE410E2N 05OE410E2Q Eurospher II 100 Diol preparative dAX columns with dynamic axial compression Column 25 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E410E2J ...E410E2N No. ...E410E2J ...E410E2N No. ...E410E2N ...E410E2Q 250 mm 25T… 25TE410E2J 25TE410E2N 25U… 25UE410E2J 25UE410E2N 25Z… 25ZE410E2N 25ZE410E2Q 150 mm 15T… 15TE410E2J 15TE410E2N 15U… 15UE410E2J 15UE410E2N 15Z… 15ZE410E2N 15ZE410E2Q 50 mm 05T… 05TE410E2J 05TE410E2N 05U… 05UE410E2J 05UE410E2N 05Z… 05ZE410E2N 05ZE410E2Q KNAUER Column Selection Guide | 21 Eurospher II • Ultra pure, spherical high performance HPLC phase based on silica gel • Cyano modification (Cyanopropyl), no endcapping, with 7 % carbon content Properties Hydrophobic and hydrophilic interaction Recommended application areas Key features For a wide range of application in normal phase mode as well as reversed phase mode (steroids, carbo-Classical phenyl phase with 7 % carbon load, hydrates polar compounds). outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications. Eurospher II 100-5 CN, 250 x 3 mm Article number: 25CE200E2J 450 BHT 400 Determination of BHT in rolling oil 350 fresh rolling oil blue spiked used rolling oil 300 250 U mA 200 150 100 50 0 0,0 0,5 1,0 1,5 2,0 2,5 3,0 3,5 4,0 4,5 5,0 5,5 6,0 6,5 7,0 Minutes \\PC-SPA-09-01\EZChrom Elite 3.3.1\Enterprise\Projects\AP 37_09 BHT in Walzöl\Data\gebrOIL_Hep999Ac001_Paper_1.dat, S 2800 [1] \\PC-SPA-09-01\EZChrom Elite 3.3.1\Enterprise\Projects\AP 37_09 BHT in Walzöl\Data\Frischöl_Hep999Ac001_Paper_1.dat, S 2800 [1] 22 | KNAUER Column Selection Guide Eurospher II Ordering information Eurospher II 100 CN Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...E200E2G ...E200E2J No. ...E200E2G ...E200E2J No. ...E200E2G ...E200E2J 250 mm 25C... 25CE200E2G 25CE200E2J 25D... 25DE200E2G 25DE200E2J 25E... 25EE200E2G 25EE200E2J with integrated 25X... 25XE200E2G 25XE200E2J 25W... 25WE200E2G 25WE200E2J 25V... 25VE200E2G 25VE200E2J precolumn 150 mm 15C... 15CE200E2G 15CE200E2J 15D... 15DE200E2G 15DE200E2J 15E... 15EE200E2G 15EE200E2J with integrated 15X... 15XE200E2G 15XE200E2J 15W... 15WE200E2G 15WE200E2J 15V... 15VE200E2G 15VE200E2J precolumn 100 mm 10C... 10CE200E2G 10CE200E2J 10D... 10DE200E2G 10DE200E2J 10E... 10EE200E2G 10EE200E2J with integrated 10X... 10XE200E2G 10XE200E2J 10W... 10WE200E2G 10WE200E2J 10V... 10VE200E2G 10VE200E2J precolumn 50 mm 05C... 05CE200E2G 05CE200E2J 05D... 05DE200E2G 05DE200E2J 05E... 05EE200E2G 05EE200E2J 5 mm precolumn P6C... P6CE200E2G P6CE200E2J P6C... P6CE200E2G P6CE200E2J P6E... P6EE200E2G P6EE200E2J cartridge Eurospher II 100 CN semi-preparative standard columns Column 8 mm ID 16 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm No. ...E200E2J ...E200E2N No. ...E200E2J ...E200E2N 250 mm 25G… 25GE200E2J 25GE200E2N 25I… 25IE200E2J 25IE200E2N 150 mm - - - 15I… 15IE200E2J 15IE200E2N 125 mm 12G... 12GE200E2J 12GE200E2N - - - 50 mm 05G… 05GE200E2J 05GE200E2N 05I… 05IE200E2J 05IE200E2N 30 mm 03G… 03GE200E2J 03GE200E2N - - - Eurospher II 100 CN preparative standard columns Column 20 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E200E2J ...E200E2N No. ...E200E2J ...E200E2N No. ...E200E2N ...E200E2Q 250 mm 25J… 25JE200E2J 25JE200E2N 25M… 25ME200E2J 25ME200E2N 25O… 25OE200E2N 25OE200E2Q 150 mm 15J… 15JE200E2J 15JE200E2N 15M… 15ME200E2J 15ME200E2N 15O… 15OE200E2N 15OE200E2Q 50 mm 05J… 05JE200E2J 05JE200E2N 05M… 05ME200E2J 05ME200E2N 05O… 05OE200E2N 05OE200E2Q Eurospher II 100 CN preparative dAX columns with dynamic axial compression Column 25 mm ID 30 mm ID 50 mm ID length & style Order 5 µm 10 µm Order 5 µm 10 µm Order 10 µm 15 µm No. ...E200E2J ...E200E2N No. ...E200E2J ...E200E2N No. ...E200E2N ...E200E2Q 250 mm 25T… 25TE200E2J 25TE200E2N 25U… 25UE200E2J 25UE200E2N 25Z… 25ZE200E2N 25ZE200E2Q 150 mm 15T… 15TE200E2J 15TE200E2N 15U… 15UE200E2J 15UE200E2N 15Z… 15ZE200E2N 15ZE200E2Q 50 mm 05T… 05TE200E2J 05TE200E2N 05U… 05UE200E2J 05UE200E2N 05Z… 05ZE200E2N 05ZE200E2Q KNAUER Column Selection Guide | 23 Eurospher II • Ultra pure, spherical high performance HPLC phase based on silica gel • Zwitterionic modification: Ammonium – sulfonic acid • 7 % carbon content Properties Separation mechanism: Hydrophilic and weak reversed phase columns; behavior is the other way electrostatic interactions round on Eurospher II HILIC compared to RP which makes it an ideal tool to enhance chromatographic Key features separations for these molecules. Modern HILIC phase with zwitterionic modification on the basis of ammonium- sulfonic acid. Outstanding mechanical and chemical stability. Tip: HILIC applications are well-suited for coupling with MS detection. The high organic content of the applied mobile phases are Recommended application areas qualified well for evaporation in the MSś Especially suited for the separation of hydrophilic, ionization source. polar and ionic analytes which are poorly retained on Eurospher II 100-5 HILIC, 150 x 3 mm ID Article number: 15CE120E2J Organic acids Water soluble vitamins 1. Maleic acid, LOD 0.016 mg/ml 1. Pyridoxal (Vitamin B6) 2. Ascobic acid, LOD 0.258 mg/ml 2. Folic acid (Vitamin B9) 3. Fumaric acid, LOD 0.032 mg/ml 3. Ascorbic acid (Vitamin C) 4. Oxalic acid, LOD 1.770 mg/ml 4. Nicotinic acid (Vitamin B3) 5. Citric acid, LOD 5.422 mg/ml 5. Thiamine (Vitamin B1) 6. Cyanocobalamine (Vitamin B12) Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...E120E2G ...E120E2J No. ...E120E2G ...E120E2J No. ...E120E2G ...E120E2J 250 mm 25C... 25CE120E2G 25CE120E2J 25D... 25DE120E2G 25DE120E2J 25E... 25EE120E2G 25EE120E2J with integrated 25X... 25XE120E2G 25XE120E2J 25W... 25WE120E2G 25WE120E2J 25V... 25VE120E2G 25VE120E2J precolumn 150 mm 15C... 15CE120E2G 15CE120E2J 15D... 15DE120E2G 15DE120E2J 15E... 15EE120E2G 15EE120E2J with integrated 15X... 15XE120E2G 15XE120E2J 15W... 15WE120E2G 15WE120E2J 15V... 15VE120E2G 15VE120E2J precolumn 100 mm 10C... 10CE120E2G 10CE120E2J 10D... 10DE120E2G 10DE120E2J 10E... 10EE120E2G 10EE120E2J with integrated 10X... 10XE120E2G 10XE120E2J 10W... 10WE120E2G 10WE120E2J 10V... 10VE120E2G 10VE120E2J precolumn 50 mm 05C... 05CE120E2G 05CE120E2J 05D... 05DE120E2G 05DE120E2J 05E... 05EE120E2G 05EE120E2J 5 mm precolumn P6C... P6CE120E2G P6CE120E2J P6C... P6CE120E2G P6CE120E2J P6E... P6EE120E2G P6EE120E2J cartridge 24 | KNAUER Column Selection Guide Eurospher II Chiral Based on ultra-high purity silica, the Eurospher II chiral Tip: Some of the most popular eluents for column materials are characterized by high HPLC (such as acetone, chloroform, DMF, enantioselectivity and loadability. DMSO, MEK, toluene, dioxane, ethyl acetate, Available in a wide range of dimensions for analytical, methylene chloride, pyridine and THF) which preparative and SMB (simulated moving bed) may be remaining in your HPLC system, can applications, Eurospher II Chiral columns are the destroy Eurospher II Chiral AM and OM phases perfect choice for even the most challenging even in small concentrations! It is highly enantioseparation tasks recommended that the HPLC system is flushed with appropriate eluents before the instal ation Since chiral separations are not very well predictable, of the chiral column. KNAUER offers a chiral screening service for your samples. Depending on the analytes, three different chiral selectors are available: Chiral HPLC USP Description Chiral selector column mode code Chiral AM NP L51 polysaccharide chiral stationary phase Amylose-tris-(3,5-dimethylphenylcarbamate) Chiral AM-R RP L51 polysaccharide chiral stationary phase Amylose-tris-(3,5-dimethylphenylcarbamate) Chiral OM NP L40 polysaccharide chiral stationary phase Cellulose-tris-(3,5-dimethylphenylcarbamate) Chiral OM-R RP L93 polysaccharide chiral stationary phase Cellulose-tris-(3,5-dimethylphenylcarbamate) Chiral NR NP n/a immobilized brush-type phase p-electron donor- and p-electron acceptor groups Chiral NR-R RP n/a immobilized brush-type phase p-electron donor- and p-electron acceptor groups Eurospher II Chiral NR Eurospher II Chiral OM Eurospher II Chiral AM Analytes need an oxygen at or near the chiral Highly versatile chiral phase Highest generality of all Eurospher II Chiral center and additionally an aromatic ring phases Elution more predictable than with Chiral AM Analyte does not need to carry an Analyte does not need to carry an aromatic and OM phases aromatic ring ring Inversion of elution order is possible No inversion of elution order possible No inversion of elution order possible Very high capacity and thus ideally suited for Scale up in the preparative HPLC range Scale up in the preparative HPLC range is preparative HPLC is possible possible Stable in all common HPLC eluents Caution! Not stable in all HPLC eluents! Caution! Not stable in all HPLC eluents! KNAUER Column Selection Guide | 25 Eurospher II Chiral • Ultra pure, spherical high performance HPLC phase for enantioseparation of racemates • Amylose modified silica gel phase with chiral selector Properties Silica gel ultra pure, > 99.99 % Key features Metal content < 10 ppm KNAUER offers these chiral stationary phases for two Particle size 3 μm, 5 μm, 10 μm different separation modes: Eurospher II Chiral AM is designed for separations in normal phase (NP) Particle form spherical mode, Eurospher II Chiral AM-R for separations in reversed phase mode. Pore size of base material 1000 Å Amylose-tris-(3,5-dimethyl- Chiral selector phenylcarbamate) for Chiral Recommended application areas AM Eurospher II Chiral AM columns are able to separate neutral, acidic and basic racemates. Eurospher II Chiral AM 5 µm, 250 x 4.6 mm ID Article number: 25EM320E2J Separation of Metoprolol, (RS)-1-(Isopropylamino)-3-[4-(2-methoxyethyl)phenoxy]propan-2-ol 26 | KNAUER Column Selection Guide Eurospher II Chiral Ordering information Eurospher II Chiral AM analytical columns Column length 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm 10 µm Order 3 µm 5 µm 10 µm No. ...M320E2G ...M320E2J ...M320E2N No. ...M320E2G ...M320E2J ...M320E2N 250 mm 25D... 25DM320E2G 25DM320E2J 25DM320E2N 25E... 25EM320E2G 25EM320E2J 25EM320E2N with integrated 25W... 25WM320E2G 25WM320E2J 25WM320E2N 25V... 25VM320E2G 25VM320E2J 25VM320E2N precolumn 150 mm 15D... 15DM320E2G 15DM320E2J 15DM320E2N 15E... 15EM320E2G 15EM320E2J 15EM320E2N with integrated 15W... 15WM320E2G 15WM320E2J 15WM320E2N 15V... 15VM320E2G 15VM320E2J 15VM320E2N precolumn 100 mm 10D... 10DM320E2G 10DM320E2J 10DM320E2N 10E... 10EM320E2G 10EM320E2J 10EM320E2N with integrated 10W... 10WM320E2G 10WM320E2J 10WM320E2N 10V... 10VM320E2G 10VM320E2J 10VM320E2N precolumn 50 mm 05D... 05DM320E2G 05DM320E2J 05DM320E2N 05E... 05EM320E2G 05EM320E2J 05EM320E2N 5 mm precolumn P6C... P6CM320E2G P6CM320E2J P6CM320E2N P6E... P6EM320E2G P6EM320E2J P6EM320E2N cartridge Eurospher II Chiral AM-R analytical columns Column length 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm 10 µm Order 3 µm 5 µm 10 µm No. ...M32RE2G ...M32RE2J ...M32RE2N No. ...M32RE2G ...M32RE2J ...M32RE2N 250 mm 25D... 25DM32RE2G 25DM32RE2J 25DM32RE2N 25E... 25EM32RE2G 25EM32RE2J 25EM32RE2N with integrated 25W... 25WM32RE2G 25WM32RE2J 25WM32RE2N 25V... 25VM32RE2G 25VM32RE2J 25VM32RE2N precolumn 150 mm 15D... 15DM32RE2G 15DM32RE2J 15DM32RE2N 15E... 15EM32RE2G 15EM32RE2J 15EM32RE2N with integrated 15W... 15WM32RE2G 15WM32RE2J 15WM32RE2N 15V... 15VM32RE2G 15VM32RE2J 15VM32RE2N precolumn 100 mm 10D... 10DM32RE2G 10DM32RE2J 10DM32RE2N 10E... 10EM32RE2G 10EM32RE2J 10EM32RE2N with integrated 10W... 10WM32RE2G 10WM32RE2J 10WM32RE2N 10V... 10VM32RE2G 10VM32RE2J 10VM32RE2N precolumn 50 mm 05D... 05DM32RE2G 05DM32RE2J 05DM32RE2N 05E... 05EM32RE2G 05EM32RE2J 05EM32RE2N 5 mm precolumn P6C... P6CM32RE2G P6CM32RE2J P6CM32RE2N P6E... P6EM32RE2G P6EM32RE2J P6EM32RE2N cartridge Eurospher II Chiral AM semi-preparative and preparative standard columns Column 10 mm ID 20 mm ID 30 mm ID length Order 5 µm 10 µm Order 5 µm 10 µm Order 5 µm 10 µm No. ...M320E2J ...M320E2N No. ...M320E2J ...M320E2N No. ...M320E2J ...M320E2N 250 mm 25H… 25HM320E2J 25HM320E2N 25J… 25JM320E2J 25JM320E2N 25M… 25MM320E2J 25MM320E2N 150 mm 15H… 15HM320E2J 15HM320E2N 15J… 15JM320E2J 15JM320E2N 15M… 15MM320E2J 15MM320E2N Eurospher II Chiral AM-R semi-preparative and preparative standard columns Column 10 mm ID 20 mm ID 30 mm ID length Order 5 µm 10 µm Order 5 µm 10 µm Order 5 µm 10 µm No. ...M32RE2J ...M32RE2N No. ...M32RE2J ...M32RE2N No. ...M32RE2J ...M32RE2N 250 mm 25H… 25HM32RE2J 25HM32RE2N 25J… 25JM32RE2J 25JM32RE2N 25M… 25MM32RE2J 25MM32RE2N 150 mm 15H… 15HM32RE2J 15HM32RE2N 15J… 15JM32RE2J 15JM32RE2N 15M… 15MM32RE2J 15MM32RE2N KNAUER Column Selection Guide | 27 Eurospher II Chiral • Ultra pure, spherical high performance HPLC phase for enantioseparation of racemates • Cellulose modified silica gel phase with chiral selector Properties Eurospher II Chiral OM is equivalent to Daicel’s well-known Chiralcel® OD* phases. Key features *) Chiralcel® OD is a brand name of Daicel Corporation. KNAUER offers these chiral stationary phases for two different separation modes: Eurospher II Chiral OM Silica gel ultra pure, > 99.99 % is designed for separations in normal phase (NP) Metal content < 10 ppm mode, Eurospher II Chiral OM-R for separations in reversed phase mode. Particle size 3 μm, 5 μm, 10 μm Particle form spherical Recommended application areas Pore size of base material 1000 Å Eurospher II Chiral OM columns are able to separate neutral, acidic and basic racemates. Cellulose-tris-(3,5-dimethyl- Chiral selector phenylcarbamate) for Chiral AM Eurospher II Chiral OM 5 µm, 250 x 4.6 mm ID Article number: 25EM370E2J Separation of Benzyl-Mandelate (Benzyl-2-Hydroxy-3-Phenylpropionate) 28 | KNAUER Column Selection Guide Eurospher II Chiral Ordering information Eurospher II Chiral OM analytical columns Column length 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm 10 µm Order 3 µm 5 µm 10 µm No. ...M370E2G ...M370E2J ...M370E2N No. ...M370E2G ...M370E2J ...M370E2N 250 mm 25D... 25DM370E2G 25DM370E2J 25DM370E2N 25E... 25EM370E2G 25EM370E2J 25EM370E2N with integrated 25W... 25WM370E2G 25WM370E2J 25WM370E2N 25V... 25VM370E2G 25VM370E2J 25VM370E2N precolumn 150 mm 15D... 15DM370E2G 15DM370E2J 15DM370E2N 15E... 15EM370E2G 15EM370E2J 15EM370E2N with integrated 15W... 15WM370E2G 15WM370E2J 15WM370E2N 15V... 15VM370E2G 15VM370E2J 15VM370E2N precolumn 100 mm 10D... 10DM370E2G 10DM370E2J 10DM370E2N 10E... 10EM370E2G 10EM370E2J 10EM370E2N with integrated 10W... 10WM370E2G 10WM370E2J 10WM370E2N 10V... 10VM370E2G 10VM370E2J 10VM370E2N precolumn 50 mm 05D... 05DM370E2G 05DM370E2J 05DM370E2N 05E... 05EM370E2G 05EM370E2J 05EM370E2N 5 mm precolumn P6C... P6CM370E2G P6CM370E2J P6CM370E2N P6E... P6EM370E2G P6EM370E2J P6EM370E2N cartridge Eurospher II Chiral OM-R analytical columns Column length 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm 10 µm Order 3 µm 5 µm 10 µm No. ...M37RE2G ...M37RE2J ...M37RE2N No. ...M37RE2G ...M37RE2J ...M37RE2N 250 mm 25D... 25DM37RE2G 25DM37RE2J 25DM37RE2N 25E... 25EM37RE2G 25EM37RE2J 25EM37RE2N with integrated 25W... 25WM37RE2G 25WM370E2J 25WM370E2N 25V... 25VM37RE2G 25VM37RE2J 25VM37RE2N precolumn 150 mm 15D... 15DM37RE2G 15DM370E2J 15DM370E2N 15E... 15EM37RE2G 15EM37RE2J 15EM37RE2N with integrated 15W... 15WM37RE2G 15WM370E2J 15WM370E2N 15V... 15VM37RE2G 15VM37RE2J 15VM37RE2N precolumn 100 mm 10D... 10DM37RE2G 10DM370E2J 10DM370E2N 10E... 10EM37RE2G 10EM37RE2J 10EM37RE2N with integrated 10W... 10WM37RE2G 10WM370E2J 10WM370E2N 10V... 10VM37RE2G 10VM37RE2J 10VM37RE2N precolumn 50 mm 05D... 05DM37RE2G 05DM370E2J 05DM370E2N 05E... 05EM37RE2G 05EM37RE2J 05EM37RE2N 5 mm precolumn P6C... P6CM37RE2G P6CM370E2J P6CM370E2N P6E... P6EM37RE2G P6EM37RE2J P6EM37RE2N cartridge Eurospher II Chiral OM semi-preparative and preparative standard columns Column 10 mm ID 20 mm ID 30 mm ID length Order 5 µm 10 µm Order 5 µm 10 µm Order 5 µm 10 µm No. ...M370E2J ...M370E2N No. ...M370E2J ...M370E2N No. ...M370E2J ...M370E2N 250 mm 25H… 25HM370E2J 25HM370E2N 25J… 25JM370E2J 25JM370E2N 25M… 25MM370E2J 25MM370E2N 150 mm 15H… 15HM370E2J 15HM370E2N 15J… 15JM370E2J 15JM370E2N 15M… 15MM370E2J 15MM370E2N Eurospher II Chiral OM-R semi-preparative and preparative standard columns Column 10 mm ID 20 mm ID 30 mm ID length Order 5 µm 10 µm Order 5 µm 10 µm Order 5 µm 10 µm No. ...M37RE2J ...M37RE2N No. ...M37RE2J ...M37RE2N No. ...M37RE2J ...M37RE2N 250 mm 25H… 25HM37RE2J 25HM37RE2N 25J… 25JM37RE2J 25JM37RE2N 25M… 25MM37RE2J 25MM37RE2N 150 mm 15H… 15HM37RE2J 15HM37RE2N 15J… 15JM37RE2J 15JM37RE2N 15M… 15MM37RE2J 15MM37RE2N KNAUER Column Selection Guide | 29 Eurospher II Chiral • Ultra pure, spherical high performance HPLC phase Key features for enantioseparation of racemates Eurospher II Chiral NR is an immobilized brush-type • Amylose modified silica gel phase with chiral phase with very broad generality and complementary selector selectivity to Eurospher II Chiral AM and OM. In many cases it is similar to Eurospher II Chiral AM or Chiral OM, but much more stable. The Chiral NR selector is Properties covalently bound, so that you can use all HPLC eluents. Silica gel ultra pure, > 99.99 % Due to its high loadability it is perfectly suited for preparative separations. Both phases can be used in Metal content < 10 ppm RP and NP mode. Eurospher II Chiral-NR-R shows Eurospher II Chiral NR: reversed elution order compared to Eurospher II 3 μm, 5 μm, 8 µm, 12 µm Chiral-NR caused by the modification with the Particle size Eurospher II Chiral NR-R: opposite enantiomeric form of the chiral selector. 8 µm, 12 µm Recommended application areas Particle form spherical Eurospher II Chiral NR columns are able to separate Pore size of base material 1000 Å analytes with an oxygen at or near the chiral center and additionally an aromatic ring. Eurospher II Chiral NR 5 µm, 250 x 4 mm ID Article number: 25DE110E2J Enantioseparation of α-ionone 30 | KNAUER Column Selection Guide Eurospher II Chiral Ordering information Eurospher II Chiral NR analytical columns Column 3 mm ID 4 mm ID 4.6 mm ID length & Order 3 µm Or- 5 µm 8 µm 12 µm Order 5 µm 8 µm 12 µm equipment No. ...E110E2J der ...E110E2J ...E110E2M ...E110E2O No. ...E110E2J ...E110E2M ...E110E2O No. 250 mm - - 25D... 25DE110E2J 25DE110E2M 25DE110E2O 25E... 25EE110E2J 25EE110E2M 25EE110E2O with integrated - - 25W... 25WE110E2J 25WE110E2M 25WE110E2O 25V... 25VE110E2J 25VE110E2M 25VE110E2O precolumn 150 mm 15C... 15CE110E2G 15D... 15DE110E2J 15DE110E2M 15DE110E2O 15E... 15EE110E2J 15EE110E2M 15EE110E2O with integrated 15X... 15XE110E2G 15W... 15WE110E2J 15WE110E2M 15WE110E2O 15V... 15VE110E2J 15VE110E2M 15VE110E2O precolumn 100 mm 10C... 10CE110E2G 10D... 10DE110E2J 10DE110E2M 10DE110E2O 10E... 10EE110E2J 10EE110E2M 10EE110E2O with integrated 10X... 10XE110E2G 10W... 10WE110E2J 10WE110E2M 10WE110E2O 10V... 10VE110E2J 10VE110E2M 10VE110E2O precolumn 50 mm 05C... 05CE110E2G 05D... 05DE110E2J 05DE110E2M 05DE110E2O 05E... 05EE110E2J 05EE110E2M 05EE110E2O 5 mm precolumn P6C... P6CE110E2G P6C... P6CE110E2J P6CE110E2M P6CE110E2O P6E... P6EE110E2J P6EE110E2M P6EE110E2O cartridge Eurospher II Chiral NR-R analytical columns Column length 4 mm ID 4.6 mm ID & equipment Order 8 µm 12 µm Order 8 µm 12 µm No. ...E11RE2M ...E11RE2O No. ...E11RE2M ...E11RE2O 250 mm 25D... 25DE11RE2M 25DE11RE2O 25E... 25EE11RE2M 25EE11RE2O with integrated 25W... 25WE11RE2M 25WE11RE2O 25V... 25VE11RE2M 25VE11RE2O precolumn 150 mm 15D... 15DE11RE2M 15DE11RE2O 15E... 15EE11RE2M 15EE11RE2O with integrated 15W... 15WE11RE2M 15WE11RE2O 15V... 15VE11RE2M 15VE11RE2O precolumn 100 mm 10D... 10DE11RE2M 10DE11RE2O 10E... 10EE11RE2M 10EE11RE2O with integrated 10W... 10WE11RE2M 10WE11RE2O 10V... 10VE11RE2M 10VE11RE2O precolumn 50 mm 05D... 05DE11RE2M 05DE11RE2O 05E... 05EE11RE2M 05EE11RE2O 5 mm precolumn P6C... P6CE11RE2M P6CE11RE2O P6E... P6EE11RE2M P6EE11RE2O cartridge Eurospher II Chiral NR semi-preparative and preparative standard columns Column 8 mm ID 10 mm ID length Order No. 8 µm 12 µm Order No. 8 µm 12 µm ...E110E2M ...E11RE2O ...E110E2M ...E11RE2O 250 mm 25G… 25GE110E2M 25GE110E2O 25H… 25HE110E2M 25HE110E2O 150 mm 15G… 15GE110E2M 15GE110E2O 15H… 15HE110E2M 15HE110E2O 20 mm ID 30 mm ID 250 mm 25J… 25JE110E2M 25JE110E2O 25M… 25ME110E2M 25ME110E2O 150 mm 15J… 15JE110E2M 15JE110E2O 15M… 15ME110E2M 15ME110E2O Eurospher II Chiral NR-R semi-preparative and preparative standard columns Column 8 mm ID 10 mm ID length Order No. 8 µm 12 µm Order No. 8 µm 12 µm ...E11RE2M ...E11RE2O ...E11RE2M ...E11RE2O 250 mm 25G… 25GE11RE2M 25GE11RE2O 25H… 25HE11RE2M 25HE11RE2O 150 mm 15G… 15GE11RE2M 15GE11RE2O 15H… 15HE11RE2M 15HE11RE2O 20 mm ID 30 mm ID 250 mm 25J… 25JE11RE2M 25JE11RE2O 25M… 25ME11RE2M 25ME11RE2O 150 mm 15J… 15JE11RE2M 15JE11RE2O 15M… 15ME11RE2M 15ME11RE2O KNAUER Column Selection Guide | 31 Eurosil Bioselect Eurosil Bioselect columns for HPLC feature a wide-A pore size of 300 Å has become the accepted pore (300 Å) stationary phase and were specifically standard for wide pore silica gels because it has been designed to separate large biomolecules, such as found to be suitable for a broad range of molecular proteins and peptides. weight proteins, peptides and oligonucleotides. In The use of larger pore silica-based bonded phases general, peptides exceeding approximately 50 amino leads to improvements in resolution, capacity and acids and oligonucleotides greater than 25 residues recovery of proteins and other biomolecules, due to are preferentially analyzed on 300 Å materials. a reduction in size exclusion mechanism and enhanced molecular diffusion rates. Physical properties Silica gel ultra pure, > 99.99 % Metal content < 10 ppm 3 μm, 5 μm, 10 μm Particle size (15 μm, 20 – 45 μm on request) Particle shape spherical Pore size 300 Å Specific surface 90 ± 5 m2/g Pore volume 0.8 ml/g Density 450 g/l Characteristics Separation mechanism of Eurosil Bioselect • Eurosil Bioselect columns are available in 4 To separate larger molecules, they need to freely modifications, useful for a wide range of separation access the interior of the pores of the packing material. tasks involving large molecules up to 100 000 Da. Therefore, the analytes’ diameter must be smaller • Strict manufacturing procedures ensure a very than the average pore diameter. narrow pore size distribution. For higher molecular weight solutes, the use of lower • Interfering anions or heavy metal ions on the silica pore size materials (60-120 Å) as typically used for the surface are not present in Eurosil Bioselect columns analysis of small molecules may result restricted because only ultra pure silica gel and high quality diffusion in the materialś pores and therewith reduced chemical modifications are employed. column efficiency. Modifications The recommended modification of Eurosil Bioselect depends on the molecular weight of the analytes. 32 | KNAUER Column Selection Guide Eurosil Bioselect • Ultra pure, spherical wide pore HPLC phase based on silica gel • Unpolar, monomeric C18 (Octadecyl) modification, endcapped • 7.5 % carbon content (conventional endcapping). Properties approx. 5000 Da. The optimized manufacturing process guarantees excellent batch–to-batch Separation mechanism: Hydrophobic interaction reproducibility. The Eurosil Bioselect sorbent is the first choice for biochromatography with silica material. Key features Eurosil Bioselect 300 C18 has been specifically Recommended application areas developed for the determination and purification of Hydrophilic biomolecules, peptides, peptide mapping peptides, proteins, and oligonucleotides up to MW after enzymatic digestion Eurosil Bioselect 300-5 C18A, 125 x 4 mm ID Eurosil Bioselect 300-5 C18A 150 x 4 mm ID Article number: 12DK184EBJ Article number: 15WK184EBJ 1. Ribonuclease 2. Cytochrome 1. Melanocyte peptide 3. Lysozyme 4. Catalase Column length 2 mm ID Tip: Caused by the larger pore size of 300 Å, Order 3 µm 5 µm Eurosil Bioselect phases are not as mechanical No. ...K181EBG ...K181EBJ stable as classical silica gel phases with up to 150 mm 15B... 15BK181EBG 15BK181EBJ 150 Å and should not be used above 200 bar 100 mm 10B... 10BK181EBG 10BK181EBJ pressure. 50 mm 05B... 05BK181EBG 05BK181EBJ Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...K181EBG ...K181EBJ No. ...K181EBG ...K181EBJ No. ...K181EBG ...K181EBJ 250 mm 25C... 25CK181EBG 25CK181EBJ 25D... 25DK181EBG 25DK181EBJ 25E... 25EK181EBG 25EK181EBJ with integrated 25X... 25XK181EBG 25XK181EBJ 25W... 25WK181EBG 25WK181EBJ 25V... 25VK181EBG 25VK181EBJ precolumn 150 mm 15C... 15CK181EBG 15CK181EBJ 15D... 15DK181EBG 15DK181EBJ 15E... 15EK181EBG 15EK181EBJ with integrated 15X... 15XK181EBG 15XK181EBJ 15W... 15WK181EBG 15WK181EBJ 15V... 15VK181EBG 15VK181EBJ precolumn 100 mm 10C... 10CK181EBG 10CK181EBJ 12D... 12DK181EBG 12DK181EBJ 12E... 12EK181EBG 12EK181EBJ with integrated 10X... 10XK181EBG 10XK181EBJ 12W... 12WK181EBG 12WK181EBJ 12V... 12VK181EBG 12VK181EBJ precolumn 50 mm 05C... 05CK181EBG 05CK181EBJ 05D... 05DK181EBG 05DK181EBJ 05E... 05EK181EBG 05EK181EBJ 5 mm precolumn P6C... P6CK181EBG P6CK181EBJ P6C... P6CK181EBG P6CK181EBJ P6E... P6EK181EBG P6EK181EBJ cartridge KNAUER Column Selection Guide | 33 Eurosil Bioselect • Ultra pure, spherical wide pore HPLC phase based on silica gel • Unpolar, monomeric C18 (Octadecyl) modification, endcapped, with 7 % carbon content (hydrophilic endcapping). Properties Separation mechanism: Hydrophobic interaction process guarantees excellent batch–to-batch reproducibility. The Eurosil Bioselect sorbent is the Key features first choice for Biochromatography with silica material. Eurosil Bioselect 300 C18A has been specifically Recommended application areas developed for the determination and purification of peptides, proteins, and oligonucleotides up to MW Hydrophilic and polar biomolecules, peptides, very approx. 5 000 Da. The optimized manufacturing polar biomolecules separated with 100 % aqueous eluents. Eurosil Bioselect 300-5 C18A 150 x 4 mm Eurosil Bioselect 300-3 C18A, 150 x 2.0 mm ID Article number: 15WK184EBJ Article number: 15BK184EBG 1. Phosphatidylinositol- 1. α-Lactalbumine 4-phosphate 2. β-Lactoglobuline 5-kinase peptide Column length 2 mm ID Order 3 µm 5 µm No. ...K184EBG ...K184EBJ 150 mm 15B... 15BK184EBG 15BK184EBJ 100 mm 10B... 10BK184EBG 10BK184EBJ 50 mm 05B... 05BK184EBG 05BK184EBJ Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...K184EBG ...K184EBJ No. ...K184EBG ...K184EBJ No. ...K184EBG ...K184EBJ 250 mm 25C... 25CK184EBG 25CK184EBJ 25D... 25DK184EBG 25DK184EBJ 25E... 25EK184EBG 25EK184EBJ with integrated 25X... 25XK184EBG 25XK184EBJ 25W... 25WK184EBG 25WK184EBJ 25V... 25VK184EBG 25VK184EBJ precolumn 150 mm 15C... 15CK184EBG 15CK184EBJ 15D... 15DK184EBG 15DK184EBJ 15E... 15EK184EBG 15EK184EBJ with integrated 15X... 15XK184EBG 15XK184EBJ 15W... 15WK184EBG 15WK184EBJ 15V... 15VK184EBG 15VK184EBJ precolumn 100 mm 10C... 10CK184EBG 10CK184EBJ 12D... 12DK184EBG 12DK184EBJ 12E... 12EK184EBG 12EK184EBJ with integrated 10X... 10XK184EBG 10XK184EBJ 12W... 12WK184EBG 12WK184EBJ 12V... 12VK184EBG 12VK184EBJ precolumn 50 mm 05C... 05CK184EBG 05CK184EBJ 05D... 05DK184EBG 05DK184EBJ 05E... 05EK184EBG 05EK184EBJ 5 mm precolumn P6C... P6CK184EBG P6CK184EBJ P6C... P6CK184EBG P6CK184EBJ P6E... P6EK184EBG P6EK184EBJ cartridge 34 | KNAUER Column Selection Guide Eurosil Bioselect • Ultra pure, spherical wide pore HPLC phase based on wide pore silica gel • Unpolar, monomeric C8 modification, endcapped • 4.5 % carbon content (conventional endcapping) Properties approx. 20 000 Da. The optimized manufacturing Separation mechanism: Hydrophobic interaction process guarantees excellent batch–to-batch reproducibility. The Eurosil Bioselect sorbent is the first choice for Biochromatography with silica material. Key features Eurosil Bioselect 300 C8 has been specifically Recommended application areas developed for the determination and purification of Hydrophilic biomolecules, peptides, peptide mapping peptides, proteins, and oligonucleotides up to MW after enzymatic digestion Column length 2 mm ID Order 3 µm 5 µm No. ...K081EBG ...K081EBJ 150 mm 15B... 15BK081EBG 15BK081EBJ 100 mm 10B... 10BK081EBG 10BK081EBJ 50 mm 05B... 05BK081EBG 05BK081EBJ Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...K081EBG ...K081EBJ No. ...K081EBG ...K081EBJ No. ...K081EBG ...K081EBJ 250 mm 25C... 25CK081EBG 25CK081EBJ 25D... 25DK081EBG 25DK081EBJ 25E... 25EK081EBG 25EK081EBJ with integrated 25X... 25XK081EBG 25XK081EBJ 25W... 25WK081EBG 25WK081EBJ 25V... 25VK081EBG 25VK081EBJ precolumn 150 mm 15C... 15CK081EBG 15CK081EBJ 15D... 15DK081EBG 15DK081EBJ 15E... 15EK081EBG 15EK081EBJ with integrated 15X... 15XK081EBG 15XK081EBJ 15W... 15WK081EBG 15WK081EBJ 15V... 15VK081EBG 15VK081EBJ precolumn 100 mm 12C... 12CK081EBG 12CK081EBJ 12D... 12DK081EBG 12DK081EBJ 12E... 12EK081EBG 12EK081EBJ with integrated 12X... 12XK081EBG 12XK081EBJ 12W... 12WK081EBG 12WK081EBJ 12V... 12VK081EBG 12VK081EBJ precolumn 50 mm 05C... 05CK081EBG 05CK081EBJ 05D... 05DK081EBG 05DK081EBJ 05E... 05EK081EBG 05EK081EBJ 5 mm precolumn P6C... P6CK081EBG P6CK081EBJ P6C... P6CK081EBG P6CK081EBJ P6E... P6EK081EBG P6EK081EBJ cartridge KNAUER Column Selection Guide | 35 Eurosil Bioselect • Ultra pure, spherical wide pore HPLC phase based on wide pore silica gel • Unpolar, monomeric C4 modification, endcapped • 2 % carbon content (conventional endcapping) peptides, proteins, and oligonucleotides up to MW approx. 100 000 Da. The optimized manufacturing Properties process guarantees excellent batch–to-batch reproducibility. The Eurosil Bioselect sorbent is the Separation mechanism: hydrophobic and hydrophilic first choice for Biochromatography with silica material. interaction Recommended application areas Key features Hydrophilic biomolecules and especially peptides Eurosil Bioselect 300 C4 has been specifically larger than 10 000- 20 000 Da developed for the determination and purification of Column length 2 mm ID Order 3 µm 5 µm No. ...K041EBG ...K041EBJ 150 mm 15B... 15BK041EBG 15BK041EBJ 100 mm 10B... 10BK041EBG 10BK041EBJ 50 mm 05B... 05BK041EBG 05BK041EBJ Column length 3 mm ID 4 mm ID 4.6 mm ID & equipment Order 3 µm 5 µm Order 3 µm 5 µm Order 3 µm 5 µm No. ...K041EBG ...K041EBJ No. ...K041EBG ...K041EBJ No. ...K041EBG ...K041EBJ 250 mm 25C... 25CK041EBG 25CK041EBJ 25D... 25DK041EBG 25DK041EBJ 25E... 25EK041EBG 25EK041EBJ with integrated 25X... 25XK041EBG 25XK041EBJ 25W... 25WK041EBG 25WK041EBJ 25V... 25VK041EBG 25VK041EBJ precolumn 150 mm 15C... 15CK041EBG 15CK041EBJ 15D... 15DK041EBG 15DK041EBJ 15E... 15EK041EBG 15EK041EBJ with integrated 15X... 15XK041EBG 15XK041EBJ 15W... 15WK041EBG 15WK041EBJ 15V... 15VK041EBG 15VK041EBJ precolumn 100 mm 12C... 12CK041EBG 12CK041EBJ 12D... 12DK041EBG 12DK041EBJ 12E... 12EK041EBG 12EK041EBJ with integrated 12X... 12XK041EBG 12XK041EBJ 12W... 12WK041EBG 12WK041EBJ 12V... 12VK041EBG 12VK041EBJ precolumn 50 mm 05C... 05CK041EBG 05CK041EBJ 05D... 05DK041EBG 05DK041EBJ 05E... 05EK041EBG 05EK041EBJ 5 mm precolumn P6C... P6CK041EBG P6CK041EBJ P6C... P6CK041EBG P6CK041EBJ P6E... P6EK041EBG P6EK041EBJ cartridge 36 | KNAUER Column Selection Guide Eurokat High performance polymer phase for the analysis of Choice of chromatographic conditions organic acids, carbohydrates and alcohols. Available The Eurokat stationary phase is designed to be in the ionic forms H, Ca, Pb, Na und Ag. applicable to a wide range of diverse chromatographic conditions. All Eurokat columns can be used at Physical properties temperatures up to 90 °C with no organic solvents. The best separation of sugars can be achieved using Particle size 10 μm, 25-56 μm between 60 to 90 °C. To extend separation ca. 8 % (H-Form) performance, it is possible to connect up to three columns of Eurokat in series. Eurokat columns require Cross-linkage ca. 6 % (Ca, Pb, Na-Form) higher cross linkage means no organic solvents and thus are environmentally smaller pore size friendly. Max. pressure up to 100 bar Max. temperature Stability up to 90 °C Eurokat polymer columns are extremely stable over the whole pH range. This is one striking advantage Characteristics compared with silica-based phases which have a Eurokat high performance polymer phases were limited lifetime at pH extremes, especially in the especially developed for the separation of organic higher pH range. Most importantly, Eurokat phases acids, carbohydrates, alcohols and even complex show extraordinary column lifetime stability and are mixtures of these compounds. not affected by aqueous solvents. Eurokat is a sulfonated cross-linked styrenedivinylbenzene copolymer available in several ionic forms Application areas (H, Ca and Pb). This particular cation exchanger is characterized by an outstanding density of functional • Carbohydrates and organic acids in softdrinks groups, making it the ideal choice for your ion and fruit juices exclusion, size exclusion and ligand exchange • Sugar substitutes chromatography. • Food preservatives • Dairy products • Urine analytic (Uric acid, Hippuric acid) • Monitoring of fermentation processes Principle of ligand exchange mechanism KNAUER Column Selection Guide | 37 Eurokat • Sulfonated cross-linked styrenedivinylbenzene Recommended application areas copolymer phase for the determination of organic Eurokat H is especially designed for the analysis of acids, carbohydrates and alcohols organic acids and even complex mixtures of acids, • H-form, 8 % cross linkage sugars and alcohols as well as sugar alcohols. Due to a differing separation process we recommend Properties the use of these type of columns only in 8 mm ID or 4 mm ID. Cation exchanger, extremly long lasting lifetime when correctly handled, best results with aqueous eluents and small amounts of anorganic acids, recommended eluent 0,01 N sulfuric acid, usage of up to 10 % organic Tip: Eurokat columns are characterized by an content in the mobile phase is possible extremely long lifetime if handled properly. Especial y pressure shocks and organic solvent contents of more than 10 % in the mobile Technical Data phase should be avoided absolutely. Sulfonated cross-linked styrenedivinylbenzene copolymer with 8 % cross linkage in the ionic form H, available in 10 μm particle size, pressure stable up to maximum 100 bar Eurokat H 10 µm, 300 x 8 mm ID Article number: 30GX340EKN 1. Isomalt 7. Glycerine 2. Lactite 8. Acetic acid 1. Oxalic acid 3. Gluconic acid 9. Ethylene glycol 2. Citric acid 4. Mannite 10. 1,2- Propandiol 3. Tataric acid 5. Xylite 11. Methanol 4. Fumaric acid 6. Threitole 12. Ethanol 5. Succinic acid 6. Adipic acid Column length 4 mm ID 8 mm ID Order No. 10 µm Order No. 10 µm ...X340EKN ...X340EKN 300 mm 30D... 30DX340EKN 30G... 30GX340EKN 120 mm - - 11G... 11GX340EKN 30 mm precolumn 03D... 03DX340EKN 03G... 03GX340EKN 38 | KNAUER Column Selection Guide Eurokat • Polymer phase for the determination of mono- and Recommended application areas disaccharides Especially designed for the analysis of small • Pb (USP L34) form, 6 % cross linkage carbohydrates up to DP 4 Properties Tip: Eurokat columns are used at high temperatures. During equilibration it is very Cation exchanger, extremely long lasting lifetime important to have an eye on the backpressure when correctly handled, best results with aqueous and to start with very low flow rates and eluents, recommended eluent is pure deionized water, increase them only slowly and stepwise after usage of up to 10 % organic content in the mobile the column has adapted to the temperature. phase is possible Technical Data Sulfonated cross-linked styrenedivinylbenzene copolymer with 6 % cross linkage in the ionic form Pb, available in 10μm particle size, pressure stable up to maximum 100 bar Eurokat Pb, 300 x 8 mm ID Eurokat Ca, 300 x 8 mm ID Article number: 30GX350EKN Article number: 30GX360EKN 1. Melezitose 1. Lactose 2. Maltose 2. Glucose 3. Xylose 3. Galactose 4. Arabinose 4. Fructose 5. Maltite 5. Mannite 6. Xylite Eurokat Pb Eurokat Ca Column 4 mm ID 8 mm ID Column 4 mm ID 8 mm ID length length Order 10 µm Order 10 µm Order 10 µm Order 10 µm No. ...X350EKN No. ...X350EKN No. ...X360EKN No. ...X360EKN 300 mm 30D... 30DX350EKN 30G... 30GX350EKN 300 mm 30D... 30DX360EKN 30G... 30GX360EKN 120 mm - - 11G... 11GX350EKN 120 mm - - 11G... 11GX360EKN 30 mm 03D... 03DX350EKN 03G... 03GX350EKN 30 mm 03D... 03DX360EKN 03G... 03GX360EKN precolumn precolumn KNAUER Column Selection Guide | 39 Eurokat • Polymer phase for the determination of sugar Recommended application areas oligomers Especially designed for the analysis of sugar • Na form, 6 % cross linkage oligomers, separates carbohydrates up to DP 8 Properties Tip: Eurokat columns are typically used with Cation exchanger, extremly long lasting lifetime when 100 % aqueous eluents. For storage, please avoid microbial growth by flushing the Eurokat correctly handled, best results with aqueous eluents, column with 0.45 µm filtered ultrapure water recommended eluent is pure deionized water, usage and storing it at 4 °C. of up to 10 % organic content in the mobile phase is possible Technical Data Sulfonated cross-linked styrenedivinylbenzene copolymer with 6 % cross linkage in the ionic form Na available in 10 μm particle size, pressure stable up to maximum 100 bar Eurokat Na, 300 x 8 mm ID Article number: 30GX210EKN 1. Fructosylnystose 2. Nystose 3. Kestose 4. Sucrose 5. Glucose 6. Fructose Column length 4 mm ID 8 mm ID Order No. 10 µm Order No. 10 µm ...X210EKN ...X210EKN 300 mm 30D... 30DX210EKN 30G... 30GX210EKN 120 mm - - 11G... 11GX210EKN 30 mm precolumn 03D... 03DX210EKN 03G... 03GX210EKN 40 | KNAUER Column Selection Guide AppliChrom® AppliChrom® ABOA columns for aqueous You decide between classical GPC/SEC SEC, organic GPC, and special columns with conventional mixed pore applications technology or AppliChrom® BPT • AppliChrom® ABOA StyDiViBe Series GPC-columns technology: for GPC analysis of organic molecules using THF, Separate oligomers and polymers with the new GPC toluene, chloroform. media line from AppliChrom® BPT synthesis techno- • AppliChrom ABOA SuperOH-P Series for aqueous logy for large range, high resolution separations with GPC/SEC-analysis of mainly neutral and anionic increased accuracy in calibration (patent pending). polymers like heparins, polysaccharides, PEO, PEG,.... • AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...) • AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,... • AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and poly-cationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan. KNAUER Column Selection Guide | 41 AppliChrom® GPC/SEC columns for biopolymers and aqueous polymers • Aqueous and highly hydrophilic polymeric base material • Particle size 7 µm • Low column bleeding for low detector noise • Large pore volume for optimal resolution • Individual pore sizes for individual molecular weight ranges • Multi-pore technology for a broad range of molecular sizes • Increased lifetime of GPC/SEC columns by combi-Measurement ranges for Applichrom ABOA SuperOH GPC/SEC nation of proprietary particle and packing columns, 300 x 8 mm, measured in water using PEG/PEO-technology standards: Porosities 100, 200, 300, 400 AppliChrom® ABOA SuperOH-P Porosity Separation Range Column dimensions Order No. 300 x 8 mm 30GE460ABL AppliChrom® ABOA SuperOH-P-100 100 Da – 2.5 kDa 50 x 8 mm precolumn 05GE460ABL 300 x 8 mm 30GH460ABL AppliChrom® ABOA SuperOH-P-200 100 Da – 20 kDa 50 x 8 mm precolumn 05GH460ABL 300 x 8 mm 30GJ460ABL AppliChrom® ABOA SuperOH-P-250 100 Da – 70 kDa 50 x 8 mm precolumn 05GJ460ABL 300 x 8 mm 30GK460ABL AppliChrom® ABOA SuperOH-P-300 1 kDa – 300 kDa 50 x 8 mm precolumn 05GK460ABL 300 x 8 mm 30GN460ABL AppliChrom® ABOA SuperOH-P-350 2.5 kDa – 1 MDa 50 x 8 mm precolumn 05GN460ABL 300 x 8 mm 30GT460ABL AppliChrom® ABOA SuperOH-P-400 10 kDa – 5 MDa 50 x 8 mm precolumn 05GT460ABL 300 x 8 mm 30GZ460ABL AppliChrom® ABOA SuperOH-P-Screening 100 Da – 1 MDa 50 x 8 mm precolumn 05GZ460ABL 42 | KNAUER Column Selection Guide AppliChrom® GPC columns for GPC analysis of organic molecules using THF, toluene, chloroform • Organic polystyrene-divinylbenzene copolymer base material of high porosity for high resolution • Particle size 5 µm • Low column bleeding for low detector noise • High purity for eliminating adsorptive effects and get pure GPC combined with high livetime • Large calibration ranges covering several decades by unique process of BPT-technology • Range of individual porosities that can be used single or in combination GPC-calibration curves AppliChrom ABOA StyDiViBe 300 x 8 mm, • GPC range of 100 Da – >10 MDa THF, 1 ml/min, PMMA standards, M[da] vs. V [ml], porosities: 35A, e 100A, 500A, 103A, 1500A-BPT, 105 A-BPT, 106 A-BPT, 107 A-BPT AppliChrom® ABOA StyDiViBE Porosity Separation Range Column dimensions Order No. 300 x 8 mm 30GA470ABJ AppliChrom® ABOA StyDiViBe-P-35A 100 Da – 2.5 kDa 50 x 8 mm precolumn 05GA470ABJ 300 x 8 mm 30GE470ABJ AppliChrom® ABOA StyDiViBe-P-100A 100 Da – 10 kDa 50 x 8 mm precolumn 05GE470ABJ 300 x 8 mm 30GL470ABJ AppliChrom® ABOA StyDiViBe-P-500A 100 Da – 30 kDa 50 x 8 mm precolumn 05GL470ABJ 300 x 8 mm 30GM470ABJ AppliChrom® ABOA StyDiViBe-P-1.000A 100 Da – 70 kDa 50 x 8 mm precolumn 05GM470ABJ 300 x 8 mm 30GU470ABJ AppliChrom® ABOA StyDiViBe-P-1.500A 100 Da – 120 kDa 50 x 8 mm precolumn 05GU470ABJ 300 x 8 mm 30GW470ABJ AppliChrom® ABOA StyDiViBe-P-10E5A-BPT 500 Da – 1.5 MDa 50 x 8 mm precolumn 05GW470ABJ 300 x 8 mm 30GY470ABJ AppliChrom® ABOA StyDiViBe-P-10E6A-BPT 10 kDa – 4 MDa 50 x 8 mm precolumn 05GY470ABJ 300 x 8 mm 30GV470ABJ AppliChrom® ABOA StyDiViBe-P-10E7A-BPT 100 kDa – 10 MDa 50 x 8 mm precolumn 05GV470ABJ KNAUER Column Selection Guide | 43 AppliChrom® Optimized for DMSO-GPC applications • Intereactionfree pure GPC/SEC • Particle size 12 µm • Easy, reliable and robust GPC/SEC-calibration by dextrans, pullulans et al. • Low column bleeding for low detectornoise for improved lightscattering or viscosity detection • 12 µm particle technology for low backpressure • Large porevolume and optimized mass transfer for polymers giving optimized resolution • Low costs caused by long livetime of column – result of combination of optimized proprietary particle and packing technology AppliChrom® ABOA DMSO-PHIL-P Porosity Separation Range Column dimensions Order No. 300 x 8 mm 30GE46MABO AppliChrom® ABOA DMSO-PHIL-P-100 100 Da – 2.5 kDa 50 x 8 mm precolumn 05GE46MABO 300 x 8 mm 30GH46MABO AppliChrom® ABOA DMSO-PHIL-P-200 100 Da – 20 kDa 50 x 8 mm precolumn 05GH46MABO 300 x 8 mm 30GJ46MABO AppliChrom® ABOA DMSO-PHIL-P-250 100 Da – 70 kDa 50 x 8 mm precolumn 05GJ46MABO 300 x 8 mm 30GK46MABO AppliChrom® ABOA DMSO-PHIL-P-300 1 kDa – 500 kDa 50 x 8 mm precolumn 05GK46MABO 300 x 8 mm 30GN46MABO AppliChrom® ABOA DMSO-PHIL-P-350 5 kDa – 1.5 MDa 50 x 8 mm precolumn 05GN46MABO 300 x 8 mm 30GT46MABO AppliChrom® ABOA DMSO-PHIL-P-400 10 kDa – 5 MDa 50 x 8 mm precolumn 05GT46MABO 300 x 8 mm 30G446MABO AppliChrom® ABOA DMSO-PHIL-P-450 50 kDa – 10 MDa 50 x 8 mm precolumn 05G446MABO 300 x 8 mm 30G646MABO AppliChrom® ABOA DMSO-PHIL-P-600 ≤ 20 MDa 50 x 8 mm precolumn 05G646MABO 300 x 8 mm 30GW46MABO AppliChrom® ABOA DMSO-PHIL-P-M 100 Da – 1 MDa 50 x 8 mm precolumn 05GW46MABO 44 | KNAUER Column Selection Guide AppliChrom® GPC columns for GPC analyses in organic solvents (DMAC or DMF) • Particle size 10 µm • Organic polymer base material of high porosity for high resolution • Wide range of porosities and particle sizes • Excellent reproducibility • High purity for eliminating adsorptive effects and get pure GPC combined with high lifetime • GPC range of 100 - 1 000 000Da • Applications: PMMA, PAN, cellulose, DMF and DMAC soluble polymers AppliChrom® ABOA DMAC-PHIL-P Porosity Separation Range Column dimensions Order No. 300 x 8 mm 30GE46DABN AppliChrom® ABOA DMAC-Phil-P-100 100 Da – 2.5 kDa 50 x 8 mm precolumn 05GE46DABN 300 x 8 mm 30GH46DABN AppliChrom® ABOA DMAC-Phil-P-200 100 Da – 20 kDa 50 x 8 mm precolumn 05GH46DABN 300 x 8 mm 30GJ46DABN AppliChrom® ABOA DMAC-Phil-P-250 100 Da – 70 kDa 50 x 8 mm precolumn 05GJ46DABN 300 x 8 mm 30GK46DABN AppliChrom® ABOA DMAC-Phil-P-300 1 kDa – 300 kDa 50 x 8 mm precolumn 05GK46DABN 300 x 8 mm 30GN46DABN AppliChrom® ABOA DMAC-Phil-P-350 1 kDa – 1 MDa 50 x 8 mm precolumn 05GN46DABN 300 x 8 mm 30GT46DABN AppliChrom® ABOA DMAC-Phil-P-400 10 kDa – >10 MDa 50 x 8 mm precolumn 05GT46DABN 300 x 8 mm 30GW46DABN AppliChrom® ABOA DMAC-Phil-P-450 100 kDa – >10 MDa 50 x 8 mm precolumn 05GW46DABN KNAUER Column Selection Guide | 45 AppliChrom® GPC/SEC-columns for aqueous analysis of neutral, anionic and additional cationic polymers For GPC / SEC analysis of • polycations • polyamines (chitosanes) • polyethylenoxides • polysaccarides • polyanions (heparins, pectins,...) AppliChrom® ABOA DMSO-PHIL-P Porosity Separation Range Column dimensions Order No. 300 x 8 mm 30GE46CABN AppliChrom® ABOA CatPhil-P-100 100 Da – 2.5 kDa 50 x 8 mm precolumn 05GE46CABN 300 x 8 mm 30GH46CABN AppliChrom® ABOA CatPhil-P-200 100 Da – 20 kDa 50 x 8 mm precolumn 05GH46CABN 300 x 8 mm 30GN46CABN AppliChrom® ABOA CatPhil-P-350 2.5 kDa – 1 MDa 50 x 8 mm precolumn 05GN46CABN 300 x 8 mm 30GT46CABN AppliChrom® ABOA CatPhil-P-400 10 kDa – 5 MDa 50 x 8 mm precolumn 05GT46CABN 46 | KNAUER Column Selection Guide Sepapure® In protein purification, a combination of different Physical properties methods is needed for a successful separation. The FPLC Sepapure® columns purity of the wanted biomolecule is increased in three steps: 100 µm Agarose particles Resin with 4 % or 6 % crosslinking Flowrate Recommended: 1 CV/ml In the “capture step” the crude biomolecule is pH Stability 3 – 9 long term extracted from major side products. In the Max. pressure 3 bar “intermediate step” further contaminations are removed, and the highly pure biomolecule is gained in the “polishing step”. For each step a different Sepapure® Desalting columns are based on Dextran method and therefore different columns are used. with an exclusion limit of 5 kDa and available in 1 ml and 5 ml cartridges. FPLC Sepapure® columns are dedicated for purification of biomolecules. Sepapure® media for Physical properties affinity chromatography (AC) and Ion-Exchange Sepapure® Desalting columns chromatography (IEX) is based on Agarose and depending on the specific purification mode Resin 20 – 50 µm Dextran particles functionalized with ligands e.g. Protein A, Ni-NTA or quaternary ammonium (Q). These FPLC media are Flowrate Recommended: 1 CV/ml available packed in 1 ml and 5 ml cartridges or as Exclusion limit 5 kDa bulk media. Max. pressure 3 bar All available bulk media at one glance: KNAUER Column Selection Guide | 47 Sepapure® FPLC media based on cross-linked agarose beads Technical data: with a mean diameter of 100 µm. Agarose beads with typical loading ranges of The FPLC media is functionalized either with Protein 1 - 30 mg/l (Protein A column) or 1 - 15 mg/ml (Protein A or Protein G ligands for the binding of antibodies G column). The maximum operating pressure the or antibody fragments. Sepapure® columns should be used at is 3 bar, while the recommended flowrate is 1 CV/ml. Properties: Recommended application areas: Sepapure® Affinity media for antibodies or antibody fragments is designed to be used with most aqueous Typically used in the first step („capture“) of an FPLC buffer systems. It is long lasting when correctly purification procedure. handled and are compatible with common CIP strategies. All Sepapure® media is stored in 20 % ethanol upon delivery. Available as prepacked 1 ml or 5 ml cartridge or as bulk material. Sepapure Protein A FF 1 ml Article number: 010X40USPZ Step 1: injection peak of human plasma and column washing Step 2: elution peak of IgG Step 3: column equilibration Column type Cartridge 1 ml 5 ml Sepapure® Protein A FF 010X40USPZ 020X40USPZ Sepapure® Protein G FF 010X40VSPZ 020X40VSPZ Resin type Media 5 ml 10 ml 25 ml 50 ml 100 ml 250 ml 1000 ml Sepapure® Protein A FF 00GX40USPZ - 00IX40USPZ 00JX40USPZ 00KX40USPZ 00NX40USPZ 00QX40USPZ Sepapure® Protein G FF - 00HX40VSPZ 00IX40VSPZ - - - - 48 | KNAUER Column Selection Guide Sepapure® FPLC media based on cross-linked agarose beads Technical data: with a mean diameter of 100 µm. Agarose beads with typical loading ranges of The FPLC media is functionalized with NTA ligands 1 - 40 mg/l (Ni-NTA) or 1 - 10 mg/ml (Glutathione). for His-Tag and glutathione ligands for GST-Tag The maximum operating pressure the Sepapure® purifications. columns should be used at is 3 bar, while the recommended flowrate is 1 CV/ml. Properties: Available as prepacked 1 ml or 5 ml cartridge or as bulk material. Sepapure® Affinity media for recombinantly tagged proteins is designed to be used with most aqueous buffer systems. It is long lasting when correctly Recommended application areas: handled and compatible with common CIP strategies. Typically used in the first step („capture“) of an FPLC All Sepapure® media is stored in 20 % ethanol upon purification procedure. delivery. Available as prepacked 1 ml or 5 ml cartridge or as bulk material. AC - Sepapure Ni-NTA FF 1 ml SEC - Sepapure Desalting 5 ml Article number: 010X39FPSZ Article number: 020X460SPZ A: flow through of unbound protein B1: elution peak of 6xHis-GFP from Ni-NTA column B2: elution peak of 6xHis-GFP from Desalting column Column / Cartridge Media Resin type 1 ml 5 ml 5 ml 25 ml 100 ml 250 ml 500 ml 1000 ml Sepapure® 010X39FPSZ 020X39FSPZ - 00IX39FPSZ 00KX39FPSZ 00NX39FPSZ 00PX39FPSZ 00QX39FPSZ Ni-NTA FF Sepapure® 010X39PSPZ 020X39PSPZ 00GX39PSPZ 00IX39PSPZ 00KX39PSPZ 00NX39PSPZ 00PX39PSPZ 00QX39PSPZ Glutathione FF KNAUER Column Selection Guide | 49 Sepapure® FPLC media based on cross-linked agarose beads Technical data: with a mean diameter of 100 µm. The agarose beads are functionalized with a The FPLC media is functionalized with different linkers quaternary ammonium for the strong anion exchanger ranging from strong anion exchange ligands to weak (Q) and with diethylaminoethyl for the weak anion cation exchange ligands. exchanger (DEAE). The strong cation exchanger is functionalized with sulphopropyl (SP) and the weak Properties: cation exchanger with carboxymethyl (CM). The maximum operating pressure the Sepapure® Sepapure® Ion-Exchange media is designed to be columns should be used at is 3 bar, while the used with most aqueous buffer systems. It is long recommended flowrate is 1 CV/ml. lasting when correctly handled and is compatible with common CIP strategies. All Sepapure® media is stored Available as prepacked 1 ml or 5 ml cartridge or as in 20 % ethanol upon delivery. bulk material. Recommended application areas: Typically used in the intermediate step of an FPLC purification procedure. Sepapure SP FF6 1ml Article number: 010X15RSPZ Sepapure CM FF6 1ml Article number: 010X15QSPZ 1. Ribonuclease A 2. Cytochrome C 3. Lysozyme Column / Cartridge Media Resin type 1 ml 5 ml 25 ml 100 ml 500 ml 1000 ml Sepapure® DEAE FF 010X15ISPZ 020X15ISPZ 00IX15ISPZ 00KX15ISPZ 00PX15ISPZ 00QX15ISPZ Sepapure® Q FF 010X15HSPZ 020X15HSPZ 00IX15HSPZ 00KX15HSPZ 00PX15HSPZ 00QX15HSPZ Sepapure® CM FF 010X15QSPZ 020X15QSPZ 00IX15QSPZ 00KX15QSPZ 00PX15QSPZ 00QX15QSPZ Sepapure® SP FF 010X15RSPZ 020X15RSPZ 00IX15RSPZ 00KX15RSPZ 00PX15RSPZ 00QX15RSPZ 50 | KNAUER Column Selection Guide Sepapure® FPLC media based on a cross-linked agarose-dextran Technical data: composite with a mean particle size of 35 µm. The Sepapure® Size Exclusion beads have a fractionation range of either 3 - 70 kDa (Sepapure® Properties: SEC 75) or 6 - 600 kDa (Sepapure® SEC 200). Sepapure® Size-Exclusion media is designedfor the The maximum operating pressure the Sepapure® preparative purification of biomolecules according columns should be used at is 3 bar. to their size. They are long lasting when correctly handled and are compatible with common CIP strategies. All Sepapure® media is stored in 20 % Recommended application areas: ethanol upon delivery. Typically used in the final step of an FPLC purification procedure. Resin type Media 25 ml 50 ml 100 ml 150 ml 250 ml 500 ml 1000 ml Sepapure® SEC 75 00IX46SSPZ 00JX46SSPZ 00KX46SSPZ 00LX46SSPZ 00NX46SSPZ 00PX46SSPZ 00QX46SSPZ Sepapure® SEC 200 00IX46TSPZ 00JX46TSPZ 00KX46TSPZ 00LX46TSPZ 00NX46TSPZ 00PX46TSPZ 00QX46TSPZ FPLC media based on dextran beads with a mean diameter of 20 - 50 µm. Properties: Sepapure Desalting 1ml Article number: 010X460SPZ Sepapure® Desalting media is designed to be used for removal of small molecules below the exclusion limit and for covenient rebuffering. It is long lasting when correctly handled and compatible with common CIP strategies. All Sepapure® media is stored in 20 % ethanol upon delivery. Available as prepacked 1 ml or 5 ml cartridge or as bulk material. Technical data: The Sepapure® dextran beads have an exclusion limit of 5 kDa. The maximum operating pressure of the Sepapure® columns is 3 bar, while the recommended flowrate is 1 CV/ml. Recommended application areas: Typically used in the final step of an FPLC purification procedure or inbetween steps for fast buffer exchange. 1. BSA 2. NaCl Column type Cartridge 1 ml 5 ml Sepapure® Desalting 010X460SPZ 020X460SPZ KNAUER Column Selection Guide | 51 Columns for SMB processes KNAUER offers purpose-built SMB sets. Additional y to the quality test of every single column, SMB sets are tested for reproducibility to ensure the appropriateness for SMB processes. For a stable SMB process, a set of identical and robust HPLC columns is essential as in every of the four zones the separation has to be identical to reach a continuous process. It is recommended to order one additional column in a SMB set (needed columns + 1) to have one column with exactly the specifications of the SMB set for determination of the parameters needed for process layout. The particle size of the separation material should be 15 µm or larger to ensure a robust purification process. While the stationary phase always depends on the application, the recommended column dimensions are mainly dependent on the amount of sample that must be purified. Column Loadability* compared to Recommended dimension analytical 250 x 4 mm column flow rate [ml/min] 250 x 4 mm 1 x (analytical reference) 1 250 x 8 mm 4 x 4 250 x 16 mm 16 x 16 250 x 20 mm 25 x 25 250 x 30 mm 56 x 55 250 x 50 mm 156 x 150 * The mass and volume loadabilities always depend on the specific sample and application as well as on the stationary phase filled in the HPLC column. The chart only gives a first clue and can be higher or lower than the given numbers. Scale-up factor (SF) calculation: SF = ID²(preparative)/ID²(analytical) 52 | KNAUER Column Selection Guide Columns for SMB processes When to use which column hardware solution? KNAUER offers stationary phases for nearly any application in normal- or reversed phase mode as well as for special purification tasks in ion exclusion and ligand exchange mode for example. Nearly all KNAUER phases are also available in larger particle sizes for easy upscaling to SMB processes. Additional y, a self packing column station for frequently changing separation task and multiple column use is available. dAX preparative column hardware • Dynamic axial compression for an extended lifetime • No formation of void volume at the column inlet possible dAX Hardware Extremely high life time Special demand for extended Excellent Reproducibility column life time More cost-intensive Refill possible Standard preparative column hardware • Flanged or standard threaded • Without axial compression Standard flanged Hardware • Simple All classical prep • cost-effective applications • versatile No refill option KNAUER Column Selection Guide | 53 3 columns with the modifications C18, C18H, C18A The column kit is available in different dimensions for a wide range of reversed phase selectivities. and particle sizes: • C18: Classical Reversed Phase columns for HPLC • Classical HPLC: 150 x 4 mm ID columns in 5 µm and UHPLC: Most often used stationary phase in particle size (Order no. A66150RP) reversed phase mode. • High resolution HPLC: 100 x 3 mm ID columns in • C18 A or AQ: Columns for polar reversed phase 3 µm particle size (Order no. A66100RP) applications with 100 % aqueous mobile phase: • UHPLC: 100 x 2 mm ID columns in 2 µm particle C18 stationary phase for the analysis of more polar size (Order no. A66101RP) and hydrophilic compounds in reversed phase mode with hydrophilic endcapping. • C18 H: Columns for more unpolar analytes in Reversed Phase mode: C18 stationary phase with a special, high efficiency or polymeric unpolar endcapping style, extended pH range pH 1-12. Sepapure® IEX Screening Kit (Order no. A66160IEX) Four different IEX resins in one package ranging from strong cation to weak anion exchangers, to find the best fitting conditions to your separation task. The IEX Screening Kit contains: • Sepapure DEAE FF (1 ml) • Sepapure Q FF (1 ml) • Sepapure CM FF (1 ml) • Sepapure SP FF (1 ml) • Sepapure Desalting (5 ml) Sepapure® His-Tag Purification Kit (Order no. A66160Ni) The essential set of columns to purify recombinant His-tagged proteins. The His-Tag Purification Kit contains: • Sepapure Ni-NTA FF (5 ml) • Sepapure Desalting (5 ml) 54 | KNAUER Column Selection Guide Column care and use Proper storage of silica based HPLC columns Silica based columns should be stored in an aprotic • The best solvent for storage of RP packings (C18, C8, solvent. C4, C1, C30, CN and Phenyl) is acetonitrile/water (50:50 v/v). The water content should not be greater Caution! Even for short-term storage, flush than 50%. out all buffer solution from the column to prevent algal growth. Make sure that all buffers • The best solvent for storage of NP packings (Silica, are washed out of the column before Diol, Nitro, Cyano and Amino) is hexan/isopropanol exchanging aqueous mobile phases by 90:10 (v/v). organic solvents. Buffer salts are not soluble • The best solvent for storage of columns used in HILIC in acetonitrile and can block capillary tubing mode (HILIC, Amino, and Silica) is acetonitrile/water and the column. (90:10 v/v) or acetonitrile/5 mM ammonium acetate, pH 5,3 (90:10 v/v). Acetonitrile content should always be greater than 90 %. Equilibration The equilibration time of a column depends on the You can reduce the equilibration time by simply column dimensions. In general, a column is equilibrated increasing the flow rate. However, make sure to flush the after 20 column volumes are flushed through it. The column with at least 10-20 column volumes to make sure equilibration times for the most important column the column is equilibrated. dimensions are summarized in the following table. Eurospher II columns with 2 μm particle size, UHPLC: Column dimension Equilibration time (Length x ID) [mm] Column volume [ml] Flow rate [ml/min] [min] 50 x 2 0.11 0.5 3 100 x 2 0.21 0.5 6 Eurospher II columns, classical HPLC: Column Column dimension Column Flow rate Equilibra- Column Equilibra- tion time dimension Flow rate tion time (Length x volume [ml/min] (Length x volume [ml/min] ID) [mm] [ml] [min] ID) [mm] [ml] [min] 250 x 4.6 2.82 1.0 28 150 x 3.0 0.72 0.6 12 150 x 4.6 1.69 1.0 17 100 x 3.0 0.48 0.6 8 100 x 4.6 1.13 1.0 12 50 x 3.0 0.24 0.6 4 50 x 4.6 0.56 1.0 6 250 x 2.0 0.53 0.25 21 250 x 4.0 2.14 1.0 22 150 x 2.0 0.32 0.25 13 150 x 4.0 1.28 1.0 13 100 x 2.0 0.21 0.25 9 125 x 4.0 1.07 1.0 11 50 x 2.0 0.11 0.25 5 250 x 3.0 1.20 0.6 20 KNAUER Column Selection Guide | 55 Regeneration of a column We recommend regenerating a column if a change in Pressure increase caused by column: backflush the peak form, retention time, resolution or an increase in column carefully to remove particle buildup from the backpressure is observed. inlet frit (connect the column outlet to the pump/injector If the system pressure begins to rise, remove the column and flush). Do not connect the column to the detector. and check the system to find whether the pressure If the column still has a high backpressure, flush the increase is being caused by the system or the column. column according to the following regeneration scheme. Pressure increase caused by system: flush system, After the regeneration procedure, re-equilibrate the exchange eluent filters, frits and/or blocked capillaries. column with the mobile phase before analyses. Regeneration scheme RP columns NP columns Columns used in HILIC mode (C18, C8, C4, C1, C30, CN and Phenyl (Silica, Diol, Nitro, Cyano and Amino (HILIC and Silica stationary phases) stationary phases) stationary phases) 20 CV* water 20 CV heptane 20 CV water 20 CV acetonitrile 5 CV isopropanol 30 CV 0.5 M ammonium acetate 5 CV isopropanol 20 CV acetonitrile 30 CV water 20 CV heptane 20 CV water 20 CV acetonitrile/water (50:50 v/v) 5 CV isopropanol 20 CV acetonitrile 20 CV acetonitrile 20 CV acetonitrile 5 CV isopropanol 20 CV acetonitrile/water (50:50 v/v) 20 CV heptane * CV = column volumes Column maintenance tips • The maximum pressure limit during operating should • The optimal temperature range for the analysis of not be exceed 90 bar. The maximum pressure for the carbohydrates is 60 – 90 °C. column material is 100 bar. • During heating process from room temperature keep • Forceful mechanical handling (bumps, shocks) as well the flow low at 0.1 ml/min for 4 mm and 0.2 ml/min as sudden temperature changes should be strictly for 8 mm columns up to 60 °C. avoided to conserve the homogeneity of the packed • Flow rate changes should also only be carried out column bed. stepwise. • Water used in preparation of the mobile phase should • Optimal flow rates are typically 0.1 – 0.2 ml/min for be either fresh double-distilled or HPLC-grade. 4 mm diameter columns and 0.4 – 0.8 ml/min for • All reagents used in sample preparation (solvents, 8 mm diameter columns. reference compounds, etc.) should be of p.a. grade. • If the column is not to be used for a longer period, Particulate matter and precipitates must be removed the inlet and outlet should be sealed with appropriate from the sample by filtration before injection. blind fittings to prevent the polymer material from • Changes in column temperature should only be drying out. For longer term storage, the column undertaken under continuous eluent flow. As a should be kept at 8 °C to avert bacterial growth. principle, drastic temperature changes should always be carried out in gradual steps. 56 | KNAUER Column Selection Guide Column care and use Column regeneration procedure Eurokat columns can be regenerated in their 3. After this cleaning process, the column is to be corresponding ionic form. Regeneration of the polymer regenerated through purging with following resin is important to maintaining the selectivity and parameters: lifetime of the column material. Flow rate 0.2 ml/min If metal ions or organic components are present in the sample, these materials may settle on the resin material ID column 8 mm or even react with the polymer, resulting in a gradual Time span 4 – 6 hours loss of column performance. Through periodic cleaning Temp. 75 – 85 °C of the column, lifetime and performance can be significantly prolonged. Cleaning Eurokat Pb: 0.25 mol/l lead nitrate eluent 1. To clean the resin, Eurokat Pb, Ca and Na columns Eurokat Ca: 0.25 mol/l calcium nitrate should be flushed for at least 4 hours (preferably Eurokat Na: 0.25 mol/l sodium chloride overnight) with double-distilled water at a flow rate or 0.1 mol/l sodium hydroxide of 0.2 ml/min (8 mm ID column) in the reverse direction Eurokat H: 0.05 mol/l sulfuric acid at 60 – 75 °C. Eurokat H columns can be cleaned in a similar manner but require 0.01 N sulfuric acid. 4. Once this procedure has been completed, the desired 2. The column should then be rinsed for an additional flow rate may be resumed gradually. The column is hour with the same cleaning eluent in the normal flow now ready for further analyses and can be put back direction at 75 – 85 °C. Maintaining this flow direction into normal use once having gradually reached the and temperature, Eurokat Pb, Ca and Na columns working temperature. should then be purged with a mixture of 10 % acetonitrile and 90 % water. Eurokat H columns should be purged with 10 % acetonitrile and 90 % 0.01 N sulfuric acid. Column using tips • In general it is recommended that a precolumn • To avoid contaminating the detector’s measurement (30 x 8 mm or 30 x 4 mm) should be used. In order to cell, neither the cleaning solution nor the regenerant eliminate undissolved particles or precipitates, the should pass through the measurement cell. sample should be filtered through a 0.45 μm filter unit. Particulate matter in the eluent is removed by installing a column inlet filter between the injector and the column. KNAUER Column Selection Guide | 57 Please visit www.knauer.net/brochures to find more information about KNAUER products and systems. AZURA® Analytical HPLC/UHPLC AZURA® Bio purification AZURA® Preparative HPLC (Document no. V7852US) (Document no. V7855US) (Document no. V7820US) AZURA® SMB systems Freezing point osmometry KNAUER Product Selection Guide (Document no. V7741US) (Document no. V7716US) (Document no. V7854US) KNAUER Brochures 58 | KNAUER Column Selection Guide Dimensions Tubing volume/length mm inches inches mm Tubing ID μl/cm μl/in 0.10 .004” 1/32” 0.8 .004” 0.08 0.21 0.12 .005” 1/16” 1.6 .005” 0.13 0.32 0.15 .006” 1/8” 3.2 .010” 0.51 1.29 0.25 .010” 1/4” 6.4 .015” 1.14 2.90 0.40 .016” 3/8” 9.5 .020” 2.03 5.15 0.50 .020” 1/2” 12.7 .025” 3.17 8.04 0.75 .030” 1” 25.4 .030” 4.56 11.58 1.00 .040” .040” 8.11 20.59 1.50 .060” .060” 18.24 46.33 2.00 .080” .070” 24.83 63.06 4.60 .180” .085” 36.61 92.99 6.00 .236” 6.40 .253” 7.00 276” 10.00 .400” Pressure Temperature MPa bar psi °C °F °C °F °C °F 5 50 725 -40 -40 65 149 170 338 10 100 1 450 -35 -31 70 158 175 347 20 200 2 901 -30 -22 75 167 180 356 30 300 4 351 -25 -13 80 176 185 365 40 400 5 802 -20 -4 85 185 190 374 50 500 7 252 -15 5 90 194 195 383 60 600 8 702 -10 14 95 203 200 392 70 700 10 153 -5 23 100 212 205 401 80 800 11 603 0 32 105 221 210 410 90 900 13 054 5 41 110 230 215 419 100 1 000 14 504 10 50 115 239 220 428 110 1 100 15 954 15 59 120 248 225 437 120 1 200 17 405 20 68 125 257 230 446 130 1 300 18 855 25 77 130 266 235 455 140 1 400 20 306 30 86 135 275 240 464 150 1 500 21 756 35 95 140 284 245 473 160 1 600 23 206 40 104 145 293 250 482 170 1 700 24 657 45 113 150 302 255 491 180 1 800 26 107 50 122 155 311 260 500 190 1 900 27 558 55 131 160 320 265 509 200 2 000 29 008 60 140 165 329 270 518 KNAUER Column Selection Guide | 59 60 | KNAUER Column Selection Guide 6.2. Claims for damages 1. Definition of terms The liability of KNAUER shall be restricted to intentional acts and The following terms and conditions apply to every order received acts of gross negligence and compensation shall only be due for by KNAUER and every delivery of goods. This holds as well in case direct, foreseeable damages. Liability for breach of a material, of contradictory buying conditions of the purchaser. Exceptions essential duty of the contract, liability because of personal injury, are only valid when confirmed by KNAUER in writing. Purchase liability according to the stipulations of the German Law on orders are only binding if confirmed by KNAUER in writing. Product Liability and liability for the lack of the condition of the contract goods guaranteed by KNAUER remain unaffected. 2. Payment Deliveries are due and payable, net, within 30 days of invoice date 7. Third party rights on industrial or other intellec-or in advance. Deductions are not allowed. 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KNAUER reserves the right to sue the customer at his commissioning, the warranty begins at the latest four weeks after principal place of business. receipt of the goods unless the supplier is responsible for delayed commissioning. This agreement shall be governed by the laws of the Federal Republic of Germany excluding the UN-Convention on the The warranty for osmometers and liquid chromatography International Sale of Goods (CISG). instruments is limited to two years, excluding glass breakage, damages due to stoppage and consumable materials such as membranes, light bulbs, columns, bushings, gaskets and valves. KNAUER's liability shall be restricted to the replacement of KNAUER Wissenschaftliche Geräte GmbH defective material or repair only. Transportation costs are borne by the customer. In case of failure of replacement or repair the cus-Hegauer Weg 38 tomer may demand a reduction in price or cancellation of the 14163 Berlin, Germany contract with respect to the defective material. The customer has to inspect the goods delivered immediately and shall immediately give written notification of any defects to KNAUER, in case of non-obvious defects within 10 working days after delivery at the These terms and conditions apply since June 1, 2016 very latest. Terms and conditions KNAUER Column Selection Guide | 61 Technical data or prices are subject to change without All trademarks are the property of their respective owners. notice. Prices may vary by country and do not include taxes, Our general terms and conditions apply: customs duties or delivery. www.knauer.net/terms . Distributed by: er .1 ap n 2 ly pd ersio S, v tally frienen 7803U m 020, V viron en ER 2U on AN © K Printed KNAUER +49 30 809727-0 Wissenschaftliche Geräte GmbH +49 30 8015010 (Fax) Hegauer Weg 38 info@knauer.net 14163 Berlin, Germany www.knauer.net Document Outline Eurospher II Eurospher II 100 C18 - USP L1 Eurospher II 100 C18 A - USP L1 Eurospher II 100 C18 H - USP L1 Eurospher II 100 C18 P - USP L1 Eurospher II 100 C8 - USP L7 Eurospher II 100 C8 A - USP L7 Eurospher II 100 Phenyl - USP L11 Eurospher II 100 NH2 - USP L8 Eurospher II 100 Si Eurospher II 100 Diol Most common HPLC modes Eurospher II 100 CN Eurospher II 100 HILIC Eurospher II Chiral Chiral selectors Characteristics of chiral separations Eurospher II Chiral AM Eurospher II Chiral OM Eurospher II Chiral NR Eurosil Bioselect Eurosil Bioselect 300 C18 - USP L1 Eurosil Bioselect 300 C18A - USP L1 Eurosil Bioselect 300 C8 - USP L7 Eurosil Bioselect 300 C4 - USP L26 Eurokat Eurokat H - USP L17 Eurokat Pb & Ca - USP L34 / USP L19 Eurokat Na AppliChrom® AppliChrom® ABOA SuperOH-P AppliChrom® ABOA StyDiViBe-P AppliChrom® ABOA DMSO-PHIL-P AppliChrom® ABOA DMAC-Phil-P AppliChrom® ABOA CatPhil-P Sepapure® Antibody Affinity Chromatography Tag-Affinity Chromatography Ion-Exchange Chromatography Size Exclusion Chromatography Desalting Columns Columns for SMB processes Column kits Eurospher II RP Analytical Column Kit Sepapure® Column Kits Column care and use Equilibration and storage of silica gel based columns like Eurospher II Regeneration of silica gel based columns like Eurospher II Eurokat column care and regeneration KNAUER brochures Conversion tables Notes Terms and conditions