Science Together KNAUER Product Selection Guide 20 20 Instruments, accessories and services for HPLC/UHPLC, Prep LC, FPLC and osmometry www.knauer.net ecycled paper Printed on r Sales If you want to learn more about our products and services or get a quote, the experts from our Sales team are happy to assist you with your request. Phone: +49 30 809727-0 (workdays 9-17h CET) Fax : +49 30 8015010 Email: sales@knauer.net Support Do you have questions about the installation or the operation of your instrument or software? International Support: Contact your local KNAUER partner for support: www.knauer.net/en/Support/Distributors-worldwide Support in Germany (Austria & Switzerland on case-to-case basis): Phone: +49 30 809727-111 (workdays 9-17h CET) Fax : +49 30 8015010 Email: support@knauer.net Made in Germany since 1962 Based in Berlin, KNAUER is a medium-sized, owner- managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography. The range includes systems and components for analytical HPLC / UHPLC, preparative HPLC, fast protein liquid chromatography (FPLC), multi-column chromatography / simulated moving bed (SMB), and osmometry. Independent and family owned The founder Dr. Herbert Knauer and his wife Roswitha are still active as advisers in the company to this day. The couple‘s daughter, Alexandra Knauer, has been managing director and owner of the company since 2000. Several awards for outstanding products and innovations as well as entrepreneurial excellence make KNAUER a „leading employer“. Comprehensive service from the manufacturer Our wide-ranging services complement our excellent instruments: The trainings are performed by experts and cover a variety of topics for beginners to experienced users. The high-performance customer support and repair service provide fast and spot on solutions. If you need support for your research project, we help you to develop applications, transfer and optimize methods or develop software. Instruments Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Assistants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Autosamplers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 Column thermostats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Detectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Fraction collectors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Degassers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 Osmometers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Accessories Pump accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 Autosampler accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Osmometry accessories and standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 Purification accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 Lab equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 PC Hardware & Software Mobile Control (Chrom) for Windows 10 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 ClarityChrom® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 PurityChrom®. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 OpenLAB® CDS EZChrom Edition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 Chromeleon™ 7.2 Drivers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 PC Hardware & Periphery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67 KNAUER Services Application Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 Chiral Column Screening Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69 KNAUER Academy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70 Research . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Worldwide Technical Services. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Development Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 Storage of instruments and systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 Configuration of your PC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 Power Cable Overview . . . . . . . . . . . . . . . . . . . 75 System Configurators Analytic HPLC/UHPLC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77 Prep HPLC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 FPLC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79 KNAUER Brochures . . . . . . . . . . . . . . . . . . . . . . 80 Conversion Tables. . . . . . . . . . . . . . . . . . . . . . . . 81 Terms & Conditions . . . . . . . . . . . . . . . . . . . . . . 82 Instruments The AZURA® Pump P 6.1L uses technology to overcome the challenges of pumping LC solvents at high pressure with low pulsation. This pump is designed to fulfil the needs for high pressure and low pressure mixing tasks. The pump can deliver flow in the range of 0.001 – 50 ml/min at pressures up to 1000 bar (depending on model and flow rate). The AZURA® binary pump contains two identical high-pressure pumps, a 2 × 2-channel solvent selection valve and the new developed AZURA® mixer, a low-volume nicrofluidic mixing device. The AZURA® quaternary pump contains one high-pressure pump and an integrated LPG mixing block with a 4 channel valve and mixer. The integrated degasser and AZURA® inline filter are completing the KNAUER offers various analytical AZURA® HPLC pump and turn this pump into a working horse in the lab. software control options: For biocompatible applications or ion chromatography, this pump is also available with a com- www.knauer.net/softwarecontrol plete metal-free design. For pump accessories Specifications see p. 36 Solvent delivery Pump type Analytical HPLC pump Delivery system Double Serial Piston Pump Pulsation compensation Active Pulsation Compensation Piston seal washing Active Wash Flow rate accuracy < 1%, measured at 5 - 50% of max. flow range using ethanol/water 10:90 Flow rate precision < 0.1% System protection Soft start, Pmin und Pmax are programmable Gradient range 0 - 100 % in 0,1 % increments Solvent selection valve HPG only Gradient formation LPG / HPG Liquid temperature range 4−60 °C (39.2−140 °F) HPG: gradient accuracy ± 0.3 % at 1 ml/min, 150 bar (ethanol/caffeine tracer) ± 1 % (5 - 95 %, measured at 0.1 – 10 ml/min, water/ caffeine tracer) HPG: gradient precision < 0.1 % RSD at 1 ml/min, 0.3% RSD overall, based on retention time at constant room temperature LPG: gradient accuracy ± 0.3 % at 1 ml/min, 150 bar (ethanol/caffeine tracer) ± 2 % (1 - 99 %, measured at 5 - 50 % of the flow range, water/caffeine tracer) LPG: gradient precision < 0.1% RSD at 1 ml/min, 0.5% RSD overall, based on retention time at constant room temperature Degasser module Degasser channels 4 channels (LPG Versions), 2 / 4 channels (HPG Versions); optional Max. flow rate/channel 10 ml/min Degassing method Gas Permeation trough Teflon(R) AF amorphous fluoropolymer membrane Degassing efficiency < 0.5 ppm dissolved O2 at 1 ml/min Degassing chamber volume 280 µl volume per channel Solvent applicability Universal, with exception of hydrochloric acid and halogenated hydrocarbons Wetted materials PEEK, Tefzel® (ETFC), Systec AF TM Communication Display Mobile Control (optional) Inputs LAN, Pin header connectors (Analog IN, Start In, Error IN) Analog inputs Flow rate, 0 - 10 V via pin header connectors Analog control input Flow Rate Level/event outputs 8 event outputs (TTL, OC, Relais) and 24 V Control LAN, Analog and event control, Mobile Control Technical parameters Leak sensor Yes Special features Pump Head is detected automatically using Radio frequency indentificaion (RFID) Ambient conditions 4-40 °C (39.2-104 °F) Air humidity below 90%, non-condensing General Power supply 100 - 240 V; 50 - 60 Hz; Maximum power consumption 100 Watt Dimensions 361 mm x 208.2 mm x 523 mm (W × H × D) Weight 14.1 kg 1 | Pumps Pumps Pump specifications Pump head 10 ml Flow rate range 0.001 - 10 ml/min Maximum delivery pressure 12500 psi / 862 bar / 86.2 MPa up to 3 ml/min / 10150 psi / 700 bar / 70 MPa up to 5 ml/min; 5800 psi / 400 bar / 40 MPa up to 10 ml/min Wetted materials GFP, Stainless Steel, FKM, PEEK, Sappire, Aluminiumoxide, Ruby, Zirconiumoxide Mixing volume 100 µl HPG, 200 µl LPG Flow rate increment 0.001 ml/min Best working conditions 0.1 - 8.0 ml/min Continuous working conditions 0.1 -4.0 ml/min Pumphead material Stainless Steel Ordering details: APH30EA AZURA® Pump P 6.1L isocratic, without degasser, with 10 ml pump head (stainless steel) APH30ED AZURA® Pump P 6.1L isocratic, without degasser, with 10 ml NP pump head (stainless steel) APH31EA Isocratic Pump P 6.1L, with degasser, with 10 ml pump head (stainless steel) and solvent selection valve APH34EA AZURA® Pump P 6.1L (LPG), with 10 ml pump head (stainless steel), degasser and mixer (200 µl) APH35EA AZURA® Pump P 6.1L (HPG), with 10 ml pump head (stainless steel), degasser and mixer (100 µl) APH35ED AZURA® Pump P 6.1L (HPG), with 10 ml NP pump head (stainless steel), degasser and mixer (100 µl) APH38EA AZURA® Pump P 6.1L (HPG), without degasser, with 10 ml pump head APH38ED AZURA® Pump P 6.1L (HPG), without degasser, with 10 ml NP pump head and mixer (100 µl) APH39EA AZURA® Pump P 6.1L (LPG), without degasser, with 10 ml pump head (stainless steel) and mixer (100 µl) Pump specifications Pump head 50 ml Flow rate range 0.01 - 50 ml/min Maximum delivery pressure 4350 psi / 300 bar / 30 MPa up to 10 ml/min; 2900 psi / 200 bar / 20 MPa up to 50 ml/min Wetted materials GFP, FKM, PEEK, Sappire, Aluminiumoxide, Ruby, Zirconiumoxide Mixing volume 200 µl Flow rate increment 0.001 ml/min Best working conditions 0.1 - 40 ml/min Continuous working conditions 0.1 - 20 ml/min Pumphead material Stainless Steel Ordering details: APH30FA AZURA® Pump P 6.1L isocratic, without degasser, with 50 ml pump head (stainless steel) APH30FD AZURA® P 6.1L Isocratic Pump APH38FA AZURA® Pump P 6.1L (HPG), without degasser, with 50 ml pump head (stainless steel) and mixer (200 µl) Pumps | 2 Pump specifications Pump head 5 ml Flow rate range 0.001 - 5 ml/min Maximum delivery pressure 14500 psi / 1000 bar / 100 MPa up to 2 ml/min, 10150 psi / 700 bar / 70 MPa up to 5 ml/min Wetted materials GFP, Stainless Steel, FKM, PEEK, Sapphire, Aluminiumoxide, Ruby, Zirconiumoxide Mixing volume 100 µl (HPG) / 200 µl (LPG) Flow rate increment 0.001 ml/min Best working conditions 0.02 - 5 ml/min Continuous working conditions 0.1 - 4 ml/min Pumphead material Stainless Steel Ordering details: APH34GA AZURA® Pump P 6.1L (LPG), with 5 ml pump head (stainless steel), degasser and mixer (100 µl) APH35GA AZURA® Pump P 6.1L (HPG), with 5 ml pump head (stainless steel), degasser and mixer (100 µl) Pump specifications Pump head 10 ml / 50 ml Flow rate range 0.001 - 10 ml/min / 0.01 - 50 ml/min Maximum delivery pressure 5800 psi / 400 bar / 40 MPa for 10 ml head, 2900 psi / 200 bar / 20 MPa for 50 ml head Wetted materials GFP, FKM, PEEK, Sappire, Aluminiumoxide, Ruby, Zirconiumoxide Mixing volume 250 µl Flow rate increment 0.001 ml/min Best working conditions for 10 ml pump heads: 0.1 - 8.0 ml/min; for 50 ml pump heads: 0.1 - 40 ml/min Continuous working conditions for 10 ml pump heads: 0.1 - 4.0 ml/min; for 50 ml pump heads: 0.1 - 20 ml/min Pumphead material Ceramic Ordering details: APH60EB AZURA® Pump P 6.1L, isocratic, without degasser, with 10 ml pump head (ceramic) APH60FB AZURA® Pump P 6.1L, isocratic, without degasser, with 50 ml pump head (ceramic) APH64EB AZURA® Pump P 6.1L (LPG), with 10 ml pump head (ceramic), degasser and mixer (250 µl) APH65EB AZURA® Pump P 6.1L (HPG), with degasser, with 10 ml pump head (ceramic) and mixer (250 µl) APH68EB AZURA® Pump P 6.1L (HPG), without degasser, with 10 ml pump head (ceramic) and mixer (250 µl) APH68FB AZURA® Pump P 6.1L (HPG), without degasser, with 50 ml pump head (ceramic) and mixer (250 µl) APH69EB AZURA® Pump P 6.1L (LPG), without degasser, with 10 ml pump head (ceramic) and mixer (250 µl) 3 | Pumps AZURA® preparative HPLC pump P 2.1L covers wide flow rate range and pressure capabilities. It has been designed for purification of mg and gram samples. The pump can deliver flow in the range of 0.01 – 1000 ml/min at pressures up to 400 bar (depending on model). The integrated automatic recognition of the pump head with RFID technology allows fast adaptions of the pump for various applications. Specifications KNAUER offers various software control options: Solvent delivery www.knauer.net/softwarecontrol Pump type Preparative HPLC pump For pump accessories Delivery system Dual Piston Pump with Al2O3 pistons parallel see p. 36 Pulsation compensation Active Pulsation Compensation Piston seal washing Active Wash Flow rate accuracy ± 2 %, measured at 5 - 50% of flow range using ethanol/water 10:90 Flow rate precision < 0.1% RSD System protection Soft start, Pmin und Pmax are programmable Gradient range 0 - 100 % Gradient formation LPG / HPG Liquid temperature range 4−60 °C (39.2−140 °F) HPG: gradient accuracy ± 2 % (5 - 95 %, measured at 5 - 50% of flow range, water/caffeine tracer) Leak management Yes HPG: gradient precision < 1 % RSD based on retention time at constant room temperature LPG: gradient accuracy ± 3 % (5 - 95 %, measured at 5 - 50% of flow range, water/caffeine tracer) LPG: gradient precision 2% RSD, based on retention time at constant room temperature Communication Display Mobile Control (optional) Inputs LAN, Pin header connectors (Analog IN, Start In, Error IN) Analog inputs Flow rate, 0 - 10 V via pin header connectors Analog control input Flow Rate Level/event outputs 8 event outputs (TTL, OC, Relais) and 24 V Control LAN, Analog and event control, Mobile Control Technical parameters Leak sensor Yes Special features Pump Head is detected automatically using Radio frequency indentificaion (RFID) Ambient conditions 10 - 40 °C (50-104 °F), Air humidity below 90%, non-condensing General Power supply 100 - 240 V; 50 - 60 Hz; Maximum power consumption 320 Watt Dimensions 361 mm x 208.2 mm x 523 mm (W × H × D) Weight 19 kg Optional accessories ternary low pressure gradient valve block, 10 - 220 ml/min, binary low pressure gradient valve block, 10 - 800 ml/min, pump head heating and cooling device Pumps | 4 Pump specifications Pump head 100 ml Flow rate range 0.01 - 100 ml/min Maximum delivery pressure 5800 psi / 400 bar / 40 MPa Wetted materials aluminium oxide (Al2O3), FKM, graphite fiber reinforced PTFE, PEEK, sapphire, stainless steel, titanium Flow rate increment 0.01 ml/min Best working conditions 1 - 80 ml/min Continuous working conditions 1 - 40 ml/min Pumphead material stainless steel / titanium Ordering details: APE20KA AZURA® Pump P 2.1L with 100 ml pump head (stainless steel) APE20KB AZURA® Pump P 2.1L with 100 ml pump head (titanium) Pump specifications Pump head 250 ml Flow rate range 0.01 - 250 ml/min Maximum delivery pressure 3260 psi / 225 bar / 22.5 MPa up to 100 ml/min, 2900 psi / 200 bar / 20 MPa up to 250 ml/min Wetted materials aluminium oxide (Al2O3), FKM, graphite fiber reinforced PTFE, PEEK, sapphire, stainless steel, titanium Flow rate increment 0.01 ml/min Best working conditions 2.5 - 200 ml/min Continuous working conditions 2.5 - 100 ml/min Pumphead material stainless steel / titanium Ordering details: APE20LA AZURA® Pump P 2.1L with 250 ml pump head (stainless steel) APE20LC AZURA® Pump P 2.1L with 250 ml pump head (titanium) 5 | Pumps Pump specifications Pump head 500 ml Flow rate range 0.01 - 500 ml/min Maximum delivery pressure 1450 psi / 100 bar / 10 MPa Wetted materials aluminium oxide (Al2O3), FKM, graphite fiber reinforced PTFE, PEEK, sapphire, stainless steel, titanium Flow rate increment 0.1 ml/min Best working conditions 5 - 400 ml/min Continuous working conditions 5 - 200 ml/min Pumphead material stainless steel / titanium Ordering details: APE20MA AZURA® Pump P 2.1L with 500 ml pump head (stainless steel) APE20MC AZURA® Pump P 2.1L with 500 ml pump head (titanium) Pump specifications Pump head 1000 ml Flow rate range 1 - 1000 ml/min Maximum delivery pressure 1080 psi / 75 bar / 7.5 MPa up to 350 ml/min, 720 psi / 50 bar / 5 MPa up to 1000 ml/min, Wetted materials aluminium oxide (Al2O3), FKM, graphite fiber reinforced PTFE, PEEK, sapphire, stainless steel, titanium Flow rate increment 0.1 ml/min Best working conditions 10 - 800 ml/min Continuous working conditions 10 - 400 ml/min Pumphead material stainless steel / titanium Ordering details: APE20NA AZURA® Pump P 2.1L with 1000 ml pump head (stainless steel) APE20NB AZURA® Pump P 2.1L with 1000 ml pump head (titanium) Ordering details: AZZ00AA LPG module for Pump P 2.1L binary up to 800 ml/min (stainless steel) AZZ00AB LPG module for Pump P 2.1L ternary up to 220 ml/min (stainless steel) AZZ10AB LPG module for Pump P 2.1L ternary up to 220 ml/min (PEEK) Pumps | 6 The AZURA® Pump P 4.1S was developed for eluent delivery up to 400 bar and for flow rates up to 50 ml/ min in HPLC and other applications where a compact easy-to-integrate pump is required. Apart from serving as a compact isocratic pump for small HPLC systems, it can also be used as a sample pump for preparative chromatography. This pump is the perfect choice for dosing applications as the exchangeable pump heads are compatible to a wide range of chemicals. Specifications KNAUER offers various Solvent delivery software control options: www.knauer.net/softwarecontrol Pump type Ultra-compact high pressure pump Delivery system Double Serial Piston Pump For pump accessories Piston seal washing Passive Wash see p. 36 Flow rate accuracy ± 2%, measured at 5 - 50% of flow range using ethanol/water 10:90 Flow rate precision ≤ 0.5% RSD, measured at 1/5 ml/min using ethanol/water 10:90 System protection Pmin und Pmax are programmable Liquid temperature range 4−60 °C (39.2−140 °F) Communication Display Yes Inputs LAN, Pin header connectors (Analog IN, Start In, Error IN), RS-232 Analog inputs 0 - 10 V Analog control input Flow Rate Control LAN, RS-232, analog, standalone Technical parameters Display Yes Ambient conditions 10-40 °C (50-104 °F) Air humidity below 90%, non-condensing General Power supply 100 - 240 V; 50 - 60 Hz; Maximum power consumption 100 Watt Dimensions 121 x 129 x 220 mm (W × H × D) Weight 2.4 kg 7 | Pumps Pump specifications Pump head 10 ml Flow rate range 0.001 - 10 ml/min Maximum delivery pressure 5800 psi / 400 bar / 40 MPa up to 10 ml/min Wetted materials Graphite fiber reinforced PTFE, FKM (FFKM for APG20EC), PEEK (PCTFE for APG20EC), sapphire, ruby, zirconium oxide, titanium and pump head material Maximum viscosity 100 mPa s (at reduced max. flow) Flow rate increment 0.001 ml/min Best working conditions 0.1 - 8.0 ml/min Continuous working conditions 0.1 - 4.0 ml/min Pumphead material Stainless steel / ceramic / Hastelloy® C Ordering details: APG20EA AZURA® Pump P 4.1S compact HPLC pump with 10 ml/min stainless steel pump head, stainless steel connections APG20EB AZURA® Pump P 4.1S compact HPLC pump with 10 ml/min ceramic pump head, PEEK connections APG20EC AZURA® Pump P 4.1S compact HPLC pump with 10 ml/min Hastelloy® C pump head, Titanium pressure sensor, Hastelloy® C connections APG20EF AZURA® Pump P 4.1S compact HPLC pump with 10 ml/min ceramic pump head, Ti connections. APG20EG AZURA® Pump P 4.1S compact HPLC pump with 10 ml/min stainless steel pump head, stainless steel connections, recommended for aqueous solutions APG20EH AZURA® Pump P 4.1S compact HPLC pump with 10 ml/min ceramic pump head, Titanium connections, recommended for aqueous solutions Pump specifications Pump head 50 ml Flow rate range 0.01 - 50 ml/min Maximum delivery pressure 2180 psi / 150 bar / 15 MPa up to 50 ml/min Wetted materials Graphite fiber reinforced PTFE, FKM (FFKM for APG20FC), PEEK (PCTFE for APG20FC), sapphire, ruby, zirconium oxide, titanium and pump head material Maximum viscosity 100 mPa s (at reduced max. flow) Flow rate increment 0.01 ml/min Best working conditions 0.5 - 40.0 ml/min Continuous working conditions 0.5 - 20 ml/min Pumphead material Stainless steel / ceramic / Hastelloy® C Ordering details: APG20FA AZURA® Pump P 4.1S compact HPLC pump with 50 ml/min stainless steel pump head, stainless steel connections APG20FB AZURA® Pump P 4.1S compact HPLC pump with 50 ml/min ceramic pump head, PEEK connections APG20FC AZURA® Pump P 4.1S compact HPLC pump with 50 ml/min Hastelloy® C pump head, Hastelloy® C connections APG20FG AZURA® Pump P 4.1S compact HPLC pump with 50 ml/min stainless steel pump head, stainless steel connections, recommended for aqueous solutions APG20FI AZURA® Pump P 4.1S compact HPLC pump with 50 ml/min ceramic pump head, PEEK connections, recommended for aqueous solutions Pumps | 8 AZURA® Pump P 2.1S was developed for eluent delivery up to 400 bar and for flow rates up to 50 ml/min in HPLC and other applications where a compact easy-to-integrate pump is required. This pump is the perfect choice for dosing applications as the exchangeable pump heads are compatible to a wide range of chemicals. Specifications Solvent delivery Pump type Ultra-compact high pressure pump KNAUER offers various Delivery system Double Serial Piston Pump software control options: Pulsation compensation No www.knauer.net/softwarecontrol Piston seal washing Passive Wash Flow rate accuracy ± 5%, measured at 5 - 50% of flow range using ethanol/water 10:90. For pump accessories ±2% at calibration point (one point calibration), see p. 36 measured at 5 - 50% of flow range Flow rate precision ≤ 0.5% RSD, measured at 1/5 ml/min using ethanol/water 10:90 System protection Imin und Imax are programmable (I ~ pressure) Liquid temperature range 4−60 °C (39.2−140 °F) Communication Display Yes Inputs LAN, Pin header connectors (Analog IN, Start In, Error IN), RS-232 Analog inputs 0 - 10 V Analog control input Flow Rate Control LAN, RS-232, analog, standalone Technical parameters Display Yes Ambient conditions 10-40 °C (50-104 °F) Air humidity below 90%, non-condensing General Power supply 100 - 240 V; 50 - 60 Hz; Maximum power consumption 100 Watt Dimensions 121 x 129 x 220 mm (W × H × D) Weight 2.3 kg 9 | Pumps Pump specifications Pump head 10 ml Flow rate range 0.001 - 10 ml/min Maximum delivery pressure 5800 psi / 400 bar / 40 MPa up to 10 ml/min Wetted materials Graphite fiber reinforced PTFE, FKM (FFKM for APG90EC), PEEK (PCTFE for APG90EC), sapphire, ruby, zirconium oxide and pump head material Maximum viscosity 100 mPa s (at reduced max. flow) Flow rate increment 0.001 ml/min Best working conditions 0.1 - 8.0 ml/min Continuous working conditions 0.1 - 4.0 ml/min Pumphead material Stainless steel / ceramic / Hastelloy® C Ordering details: APG90EA AZURA® Pump P 2.1S compact HPLC pump with 10 ml/min stainless steel pump head APG90EB AZURA® Pump P 2.1S compact HPLC pump with 10 ml/min ceramic pump head APG90EC AZURA® Pump P 2.1S compact HPLC pump with 10 ml/min Hastelloy® C pump head APG90EG AZURA® Pump P 2.1S compact HPLC pump with 10 ml/min stainless steel pump head, recommended for aqueous solutions Pump specifications Pump head 50 ml Flow rate range 0.01 - 50 ml/min Maximum delivery pressure 2180 psi / 150 bar / 15 MPa up to 50 ml/min Wetted materials Graphite fiber reinforced PTFE, FKM (FFKM for APG90FC), PEEK (PCTFE for APG90FC), sapphire, ruby, zirconium oxide and pump head material Maximum viscosity 100 mPa s (at reduced max. flow) Flow rate increment 0.01 ml/min Best working conditions 0.5 - 40.0 ml/min Continuous working conditions 0.5 - 20 ml/min Pumphead material Stainless steel / ceramic / Hastelloy® C Ordering details: APG90FA AZURA® Pump P 2.1S compact HPLC pump with 50 ml/min stainless steel pump head APG90FB AZURA® Pump P 2.1S compact HPLC pump with 50 ml/min ceramic pump head APG90FC AZURA® Pump P 2.1S compact HPLC pump with 50 ml/min Hastelloy® C pump head APG90FG AZURA® Pump P 2.1S compact HPLC pump with 50 ml/min stainless steel pump head, recommended for aqueous solutions Pumps | 10 Docking station for pumps, valves and detectors The Assistant ASM 2.2L is a docking station for up to three compact devices. Valves, pumps and UV detectors can be combined in one housing. The plug-in modules are removed by loosening four screws allowing the user to exchange modules in case of service within minutes. Likewise, the configuration of the LC system can be adapted to new requirements. Routine maintenance work e.g. replacing the lamp of a detector are easily performed by the user. Depending on the integrated modules the assistant fulfills many different tasks like eluent delivery, detection, sample and solvent selection, sample injection, column switching or fraction collection. An assistant including a pump, injection valve, and detector features a complete, compact chromatographic system. As a part of a larger system, the ASM 2.2L is extremely versatile in analytical, preparative and continuous liquid chromatography. Select your desired plug-in modules for the left, middle and right position in the assistant KNAUER offers various and you will get your perfect assistant for chromatography and beyond. software control options: www.knauer.net/assistants www.knauer.net/softwarecontrol Specifications General Power supply 100 – 240 V, 50 – 60 Hz, maximum 130 W Dimensions 361 x 208 x 523 mm (W x H x D) Weight about 17 kg (depending on integrated modules) Leak sensor Yes Ambient conditions Temperature range: 4 - 40 °C, 39,2 - 104 °F Humidity: 10 - 90 % not condensing Communication Interfaces LAN Control Mobile Control, Software Inputs Error (IN), Start (IN), Autozero, 0–10 V Analog IN Outputs Event 1–2, Error (OUT) (OC), + 5 V, + 24 V Analog inputs Integrator output (detector signal) Software functions Assistant configuration: The ASM 2.2L is supported as complete device. Modules are addressed via the assistant. ClarityChrom® OpenLAB® Mobile Control (version 6) Two pumps (independent) yes no yes Fraction valve one one yes, one valve Injection module* no no yes, but part of a method Single device configuration: The ASM 2.2L is not supported as device. Integrated modules are addressed as separate devices via IP port. ClarityChrom® OpenLAB® PurityChrom® Two pumps (independent) no yes yes Fraction valve no cascading one (Multi valve fraction collector) Injection module* yes, but part of a method yes, fully automatic module yes, but part of a method with trigger for data acquisition * An injection module is a combination of one pump and one 6 port 2 position valve. Assistants 11 | Assistants Plugin modules The Assistant ASM 2.2L can be equipped with following plug-in modules. Use the web-based configurator to customize your assistant: www.knauer.net/assistantconfigurator. Select the chosen plug-in modules for the left, middle and right position of the assistant to receive the article number of your assistant variant. Configuration note An assistant with following configuration is not allowed: � more than two pump modules - a high-pressure gradient is not supported � more than one UV detector � without a plug-in module Basic device ASM 2.2L basic device AY* Plug-in modules Basic plug-in modules Article number of modules integrated in ASM 2.2L* Empty module G2022 AZURA® Valve Unifier VU 4.1** EWA04 AZURA® UV Detector UVD 2.1S EDA03XA AZURA® Valve Unifier VU 4.1** AZURA® UV Detector UVD 2.1S, fiber optics EDA07XA Compact pump without pressure sensor AZURA® Pump P 2.1S, 10 ml, stainless steel DPG92EA AZURA® Pump P 2.1S, 10 ml, Hastelloy C DPG92EC AZURA® Pump P 2.1S, 10 ml, ceramic DPG92EB AZURA® Pump P 2.1S, 50 ml, stainless steel DPG92FA AZURA® Pump P 2.1S, 50 ml, Hastelloy C DPG92FC AZURA® Pump P 2.1S, 50 ml, ceramic DGP92FB Compact pump with pressure sensor AZURA® Pump P 4.1S, 10 ml, stainless steel DPG22EA AZURA® Detector UVD 2.1S AZURA® Pump P 4.1S, 10 ml, stainless steel, normal phase DPG22ED AZURA® Pump P 4.1S, 10 ml, ceramic DPG22EB AZURA® Pump P 4.1S, 50 ml, stainless steel DPG22FA AZURA® Pump P 4.1S, 50 ml, stainless steel, normal phase DPG22FD AZURA® Pump P 4.1S, 50 ml, ceramic DPG22FB AZURA® Pump P 4.1S, 50 bar, 10 ml, stainless steel DPG12EA AZURA® Pump P 4.1S, 50 bar, 10 ml, ceramic DPG12EB AZURA® Pump P 4.1S, 50 bar, 50 ml, stainless steel DPG12FA AZURA® Pump P 4.1S, 50 bar, 50 ml, ceramic DPG12FB AZURA® Pump P 4.1S * No article numbers for direct order. Use the listed article numbers in the configurator to find your desired module combination: www.knauer.net/assistantconfigurator. To purchase a single plug-in module, replace the first letter of its article number for an “A”. Example: EWA04 is the valve drive which is integrated in an assistant. Use the article number AWA04 to order it as a single module. An exception is the empty module which can separatly be ordered as AG2022. ** Note that valves V 4.1 must be ordered in addition to the valve drive VU 4.1. For valves, see p. 33 Accessories Column holder - replacing empty module AG2022B Column holder Assistants | 12 Autosamplers The Autosampler AS 6.1L can inject from up to 768 positions when equipped with microti-ter plates (either high or low formats) or from up to 108 standard 2 ml sample vials. Sample carryover is significantly minimized thanks to a highly-effective interior and exterior needle wash procedure. This autosampler is also fast and flexible: one complete sample injection cycle takes less than one minute, including needle wash. Three different injection modes are supported; “full loop filling” (highest reproducibility), “partial loop filling” (variable volumes, e.g. for dilution series) and “μl pickup” (loss-free injection of extremely small sample volumes), allowing the user to optimize sample usage. The headspace pressure function prevents bubbles from forming in the vial during sample uptake. Precolumn derivatization is supported. For high-pressure injections of up to 1240 bar, the autosampler is equipped with a so-called ILD™ valve (Intermediate Loop Decompression). This valve consists of a rotor-stator combination KNAUER offers various and includes a central port for depressurizing. For highpressure applications, the sample loop software control options: is depressurized prior to receiving the sample. This way, the sample is not diluted with solvent. www.knauer.net/softwarecontrol Because the valve is switched extremely fast, pressure spikes are reduced. Analyses are more precise and wear of the column is reduced. For autosampler accessories see p. 40 Specifications Sample injection Maximum back pressure see device versions Vial/plate dimensions max. plate/vial height: 47 mm (incl. septa or capmat) Injection volume range 0.1 µl - 10 ml depending on sample loop Sample loop see device versions Dispenser syringe see device versions Headspace pressure built-in compressor, only for sample vials with septum Switching time inj. valve < 100 ms Piercing needle precision ± 0.6 mm Sample tray cooling optional Vial detection missing vial/well plate detection by sensor Needle wash programmable: wash between injections and wash between vials Wetted materials Tefzel® (ETFE), Glass, Teflon® (PTFE), Kel-F® (PCTFE), stainless steel, PEEK, Vespel Analytical performance Injection modes full loop filling, partial loop filling and microliter pickup Injection precision full loop filling: < 0.3 % RSD partial loop injection at injection volumes>5 µl: <0.5% RSD microliter pickup at injections>5 µl: <1.0% RSD Sample carryover < 0,01 % under typical conditions using needle wash; < 0.003 % under special conditions with extended needle wash Injections per vial max. 9 injections Injection cycle time minimum 7 s from the same vial, 14 s from different vials;< 60 s for>100 µl sample injection in all injection modes, incl. 300 µl needle wash Analysis time max. 9 h, 59 min, 59 s Communication Inputs 2 programmable TTL inputs (next injection, freeze, stop) Outputs 1 programmable relay output (inject marker, auxiliary, alarm) Control Ethernet (LAN) Interfaces LAN, analog Technical parameters Ambient conditions temperature range: 10–40 °C; 50–104 °Fair humidity: 20 - 80% General Power supply 95–240 V AC Dimensions 361 x 375 x 570 mm (W x H x D) Weight 30 kg 13 | Autosamplers Device versions HPLC+ UHPLC Bio Prep Maximum back pressure 860 bar 1240 bar 345 bar 200 bar Sample needle 15 µl 15 µl 15 µl 60 µl Dispenser syringe 250 μl 250 μl 250 μl 2500 μl Buffer tubing 500 μl 500 μl 1000 μl 2000 μl Sample loop 100 μl, 0.4 mm ID 10 μl, 0.18 mm ID 100 μl, 0.4 mm ID 10 ml Order number AAA50AA AAA10AA AAA20AA AAA40AA Order number (cool/heat option) AAA51AA AAA11AA AAA21AA AAA41AA* * also available as biocompatible version: AAA31AA Ordering details: AAA50AA AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 860 bar AAA51AA AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 860 bar, with sample cooling/heating AAA10AA AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 1240 bar AAA11AA AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 1240 bar, with sample cooling/heating AAA20AA AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 345 bar, with biocompatible flow path AAA21AA AZURA® Autosampler AS 6.1L analytical HPLC autosampler, 345 bar, with biocompatible flow path and sample cooling/heating AAA40AA AZURA® Autosampler AS 6.1L preparative HPLC autosampler, 200 bar AAA41AA AZURA® Autosampler AS 6.1L preparative HPLC autosampler, 200 bar, with sample cooling/heating AAA31AA AZURA® Autosampler AS 6.1L preparative HPLC autosampler, 200 bar, with biocompatible flow path and sample cooling/heating Autosamplers | 14 The AZURA® CT 2.1 is a price attractive basic column thermostat. It allows temperature control in the range of 5 °C and 85 °C and thus is appropriate for most HPLC applications. For advanced purification and analysis purposes, the oven can optionally be equipped with an eluent pre-heating cartridge. This ensures even more constant separation conditions leading to higher selectivity and an improved peak shape. The instrument operates with a microprocessor controlled Peltier element for precise temperature settings. In combination with its high temperature stability, this allows programming of linear as well as non-linear temperature gradients. Specifications Thermostatting Heating and cooling system microprocessor controlled Peltier element for heating and cooling, KNAUER offers various fan supported 2-way air circulation software control options: Temperature range 5–85 °C www.knauer.net/softwarecontrol Heating/cooling rate 2 °C/min Temperature accuracy ± 0.2 °C Temperature stability ± 0.1 °C Column compartment Column dimensions max. number max. length* max. outer diameter* matching column 8 160 mm 12 mm 125 mm x 4.6 mm ID with precolumn 4 325 mm 12 mm 300 mm x 4.6 mm ID 1 325 mm 35 mm 300 mm x 16 mm ID *total outer dimensions of the column including screw caps Dimensions, internal 90 x 390 x 47 mm (W x H x D) Safety self-check and auto-calibration at power-on, selectable turn-off temperature Leak sensor gas sensor, adjustable sensitivity, acoustic signal, turn-off switch Communication Control optional for Stand-alone functionality: - keyboard unit with LCD - Mobile Control Interfaces LAN Interface General Power supply 90–230 V, 50–60 Hz, 100 W Dimensions 150 x 470 x 310 mm (W x H x D) Weight 8.4 kg Other Optional accessories Cartridge for Eluent pre-heating for capillary with an ID of 0.1 or 0.18 mm Ordering details: Device A05852 Column thermostat AZURA® CT 2.1 for constant temperatures and reproducible results Accessories A05852-3 Cartridge for eluent Pre-heating ID 0.1 mm, ~5.5 µl A05852-2 Cartridge for eluent Pre-heating ID 0.18 mm, ~18 µl A05852-1 Keyboard unit for stand-alone operation Column thermostats 15 | Column Thermostats Detectors The AZURA® DAD 6.1L is a high-end diode array detector (DAD) which combines outstanding performance with easy handling. A wide range of easily exchangeable flow cells make this device the right choice for fast, standard analytical, semi-preparative and preparative separations with bio-compatible or stainless steel wetted parts. Key features State-of-the-art total reflection flow cells (LightGuide technology) are available for this detector • Wide application range providing maximum light throughput (due to total internal reflection) with minimal peak disper- • Large choice of flow cells sion (due to small cell volume) to guarantee an optimized signal to noise (S/N) ratio. • Fiber optics adapter available An optional fiber optics adapter offers the possibility to separate the flow cell spatially from the • Attractively priced device and thus provides enhanced security for hazardous, explosive or toxic work processes, as • Made in Germany well as protecting the device from leakages at high flow rates. The newly developed optical unit with KNAUER Polka-Dot technology and intelligent temperature management ensure maximum sensitivity combined with minimal baseline drift over the KNAUER offers various whole spectrum. software control options: Furthermore, easy frontal access and improved safety features enable effortless lamp replace- www.knauer.net/softwarecontrol ment. This eases maintenance and guarantees short downtimes. The DAD 6.1L comes installed with a high brightness deuterium and tungsten halogen lamps, For detector accessories which cover a wavelength range from 190 to 1000 nm. see p. 42 Specifications Detection Detector type Diode array detector Number of diodes 1024 Pixel pitch 0.8 nm/diode Detection channels 8 (Digital)/4 (Analog) Light source High brightness deuterium (D²) lamp and halogen lamp with integrated GLP chip Wavelength range 190 - 1000 nm Spectral bandwidth < 3.5 nm at H line (FWHM) /Note: digital bandwidth 1 - 32 nm α Wavelength accuracy ± 1 nm Noise ± 3.5 µAU at 254 nm (ASTM E1657-98) Drift 300 µAU/h at 254 nm (ASTM E1657-98) Linearity > 2.5 AU at 274 nm (ASTM E1657-98) Maximum data rate 100 Hz (LAN)/12.5 Hz (analog) Flow cell Not included (see Accessories / Spare parts) Time constants 0.00 / 0.01 / 0.02 / 0.05 / 0.1 / 0.2 / 0.5 / 1.0 / 2.0 / 5.0 / 10.0 s Integration time Automatic Wavelength verification Internal holmium filter and deuterium lines Leak sensor Yes Communication Inputs Error (IN), Start (IN), Autozero Outputs Events 1 - 2 (Relay and TTL compatible, respectively), Error (OUT), + 5 V, Valve + 24 V, Valve (OUT) Analog outputs 4 x 0 - 5 V, 20 bit, offset adjustable Control Mobile Control, software, event control, analog, terminal protocol Interfaces LAN (RJ-45), USB (service only), multi-pin connector, analog (RCA cinch connector) Technical parameters GLP Detailed report including lamp recognition, operating hours, lamp operating hours, number of lamp ignitions Display Mobile Control (optional) Ambient conditions Temperature range: 4 - 40 °C, 39.2 - 104 °F, Humidity: below 90 % non-condensing Detectors | 16 General Power supply 100 – 240 V, 50 – 60 Hz, 75 W Dimensions 361 x 158 x 523 mm (W x H x D) Weight 13.8 kg Ordering details: Device ADC11 AZURA® Detector DAD 6.1L Diode array detector DAD 6.1L without flow cell 190 - 1000 nm, incl. test cell Accessories AMC19XA 10 mm path length, 2µl, 1/16”, 50 bar, LightGuide Flow cell cartridge for AZURA® Detector DAD/MWD AMD59XA 50 mm path length, 6µl, 1/16”, 50 bar, High Sensitivity LightGuide Flow cell cartridge for AZURA® Detector DAD/MWD AMC38 10 mm path length, 10µl, 1/16”, 300 bar, PressureProof Flow cell cartridge for AZURA® Detector DAD/MWD AMB18 3 mm path length, 2µl, 1/16”, 300 bar, PressureProof Flow cell cartridge for AZURA® Detector DAD/MWD AZL01 HBST deuterium lamp for AZURA® Detector DAD 6.1L AZL02 Halogen lamp for AZURA® Detector DAD 6.1L AMKX8KIT Fiber optics adapter kit for AZURA® Detector DAD/MWD, with fiber optic cables (1x 400 mm and 1x 750 mm) and mounting bracket AMLX8 Test cell for AZURA® Detector DAD/MWD The AZURA® DAD 2.1L is a highly competitive diode array detector which combines high performance with easy handling at an affordable price. A wide range of easily exchangeable cartridge flow cells make this device the right choice for fast, standard analytical, semi-preparative and preparative separations with bio-inert or stainless steel wetted parts. Key features State-of-the-art total reflection flow cells (LightGuide technology) are available for this device pro- • Wide application range viding maximum light throughput (due to total internal reflection) with minimal peak dispersion • Large choice of flow cells (due to the small cell volume) to guarantee an optimized S/N ratio. • Fiber optics adapter available An optional fiber optics adapter offers the possibility to separate the flow cell spatially from the • Leak management device and thus provides enhanced security for hazardous, explosive or toxic work processes, as • Made in Germany well as protecting the device from leakages at high flow rates. The newly developed optical unit and intelligent temperature management ensure for maximum sensitivity combined with minimal baseline drift. KNAUER offers various Furthermore, easy frontal access and improved safety features enable effortless lamp replace-software control options: ment. This eases maintenance and guarantees short downtimes. www.knauer.net/softwarecontrol The DAD 2.1L comes installed with a deuterium lamp which covers a wavelength range from 190 to 700 nm. For detector accessories see p. 42 17 | Detectors Specifications Detection Detector type Diode array detector Number of diodes 256 Pixel pitch 2 nm/diode Detection channels 8 (Digital)/4 (Analog) Light source Deuterium (D²) lamp with integrated GLP chip Wavelength range 190 - 700 nm Spectral bandwidth <10 nm at H line (FWHM) /Note: digital bandwidth 1 - 32 nm α Wavelength accuracy ± 1 nm Noise ± 5 µAU at 254 nm (ASTM E1657-98) Drift 400 µAU/h at 254 nm (ASTM E1657-98) Linearity > 2.0 AU at 274 nm (ASTM E1657-98) Maximum data rate 100 Hz (LAN)/12.5 Hz (analog) Flow cell Not included (see Accessories / Spare parts) Time constants 0.00 / 0.01 / 0.02 / 0.05 / 0.1 / 0.2 / 0.5 / 1.0 / 2.0 / 5.0 / 10.0 s Integration time Automatic Wavelength verification Internal holmium filter and deuterium lines Leak sensor Yes Communication Inputs Error (IN), Start (IN), Autozero Outputs Events 1 - 2 (Relay and TTL compatible, respectively), Error (OUT), + 5 V, Valve + 24 V, Valve (OUT) Analog outputs 4 x 0 - 5 V, 20 bit, offset adjustable Control Mobile Control, software, event control, analog, terminal protocol Interfaces LAN (RJ-45), USB (service only), multi-pin connector, analog (RCA cinch connector) Technical parameters GLP Detailed report including lamp recognition, operating hours, lamp operating hours, number of lamp ignitions Display Mobile Control (optional) Ambient conditions Temperature range: 4 - 40 °C, 39.2 - 104 °F, Humidity: below 90 % noncondensing General Power supply 100 – 240 V, 50 – 60 Hz, 75 W Dimensions 361 x 158 x 523 mm (W x H x D) Weight 12.2 kg Ordering details: Device ADC01 AZURA® Detector DAD 2.1L Diode array detector DAD 2.1L without flow cell 190 - 700 nm, incl. test cell ADB01 AZURA® Detector MWD 2.1L Multiwavelength detector MWD 2.1L, without flow cell 190 - 700 nm, incl. test cell Accessories AMC19XA 10 mm path length, 2µl, 1/16”, 50 bar, LightGuide Flow cell cartridge for AZURA® Detector DAD/MWD AMD59XA 50 mm path length, 6µl, 1/16”, 50 bar, High Sensitivity LightGuide Flow cell cartridge for AZURA® Detector DAD/MWD AMC38 10 mm path length, 10µl, 1/16”, 300 bar, PressureProof Flow cell cartridge for AZURA® Detector DAD/MWD AMB18 3 mm path length, 2µl, 1/16”, 300 bar, PressureProof Flow cell cartridge for AZURA® Detector DAD/MWD A5193 Deuterium lamp, replacement, for S25 20, 10D, 40D, UVD 2.1S, UVD 2.1L, DAD 2.1L, MWD 2.1L AMKX8KIT Fiber optics adapter kit for AZURA® Detector DAD/MWD, with fiber optic cables (1x 400 mm and 1x 750 mm) and mounting bracket AMLX8 Test cell for AZURA® Detector DAD/MWD Detectors | 18 The AZURA® UV/VIS Detector UVD 2.1L is a competitively priced HPLC spectrophotometer for routine HPLC applications including fast LC methods. Besides offering excellent technical specifications, this robust detector features a highly flexible and compact design. The UVD 2.1L comes with an installed deuterium lamp which covers a wavelength range from 190 to 750 nm. Due to a smart design the flow cell is easily accessible and can be changed very quickly. You can Key features choose between a wide range of flow cells for analytical or preparative LC applications with flow • Large choice of flow cells rates from 10 μl/min up to 10 l/min. • Leak management • 55 years experience Specifications • Made in Germany Detection Detector type Variable single wavelength UV detector KNAUER offers various Detection channels 1 software control options: Light source Deuterium (D²) lamp with integrated GLP chip www.knauer.net/softwarecontrol Wavelength range 190 - 750 nm Spectral bandwidth 11 nm at H line (FWHM) For detector accessories α Wavelength accuracy ± 2.5 nm see p. 42 Wavelength precision 0.3 nm (ASTM E275-93) Noise ± 15 µAU at 254 nm (ASTM E1657-98) Drift 300 µAU/h at 254 nm (ASTM E1657-98) Linearity > 2.0 AU at 274 nm (ASTM E1657-98) Maximum data rate 50 Hz (LAN)/20 Hz (Analog) Flow cell Not included (see Accessories / Spare parts) Time constants 0.0 / 0.1 / 0.2 / 0.5 / 1.0 / 2.0 / 5.0 / 10.0 s Integration time Automatic Leak sensor Yes Communication Inputs Error (IN), Start (IN), Autozero, 0 - 10 V Analog IN Outputs Events 1 - 3, + 5 V, 24 V Valve Analog outputs 1 x 0 - 5 V scalable, 20 bit, offset adjustable Control Digital: LAN, remote connector/Analog: wavelength control/Manual: Mobile Control (optional) Programming Timed: wavelength, events, fraction valve, links, wake up (program, link); 9 programs, 50 program lines Technical parameters GLP Detailed report including lamp recognition, operating hours, lamp operating hours, number of lamp ignitions Display Mobile Control (optional) Ambient conditions Temperature range: 4 - 40 °C, 39.2 - 104 °F, Humidity: below 90 % noncondensing General Power supply 100 - 240 V, 50 - 60 Hz, 65 W Dimensions 361 x 158 x 523 mm (W x H x D) Weight 5.9 kg 19 | Detectors Ordering details: Device ADA01XA AZURA® Detector UVD 2.1L with deuterium lamp without flow cell, incl. test cell ADA04XA AZURA® Detector UVD 2.1L Fiber Optics Version with deuterium lamp without flow cell Accessories A4061XB 10 mm path length, 10 µl, 1/16”, 300 bar, stainless steel, with heat exchanger one sided inlet and outlet, classical KNAUER flow cell A4042 3 mm path length, 2 µl, 1/16”, stainless steel, classical KNAUER flow cell A5193 Deuterium lamp, replacement, for S25 20, 10D, 40D, UVD 2.1S, UVD 2.1L, DAD 2.1L, MWD 2.1L A4126 Test cell Holmium Oxid Filter A4146 Test cell, WG 280 filter stray light A4123 Test cell The AZURA® UVD 2.1S is a highly competitive single variable wavelength UV detector for HPLC. It offers excellent technical specifications for routine laboratory work. With its small footprint, it is one of the smallest detectors for HPLC on the market. The UVD 2.1S comes in the novel small AZURA® housing. The installed deuterium lamp covers a wavelength range from 190 to 500 nm. The UV detector can be controlled with OpenLab EZChrom Edition®, ChromGate®, PurityChrom Bio and ClarityChrom® software, as well as from the front panel (stand-alone operation), via LAN, via RS-232, or through analog input/output; allowing it to be integrated into almost any LC system. Due to a smart design, the flow cell is easily accessible and can be changed very quickly. You can Key features choose between a wide range of flow cells for analytical or preparative LC applications with flow rates from 10 μl/min up to 10 l/min. • Compact • Large choice of flow cells Also available as a module for the new AZURA® ASM 2.2L. • 55 years experience • Made in Germany Specifications Detection Detector type Variable single wavelength UV detector KNAUER offers various Detection channels 1 software control options: Light source Deuterium (D²) lamp with integrated GLP chip www.knauer.net/softwarecontrol Wavelength range 190 - 500 nm Spectral bandwidth 13 nm at H line (FWHM) For detector accessories α Wavelength accuracy ± 3 nm see p. 42 Wavelength precision 0.7 nm (ASTM E275-93) Noise ± 20 µAU at 254 nm (ASTM E1657-98) Drift 300 µAU/h at 254 nm (ASTM E1657-98) Linearity > 2.0 AU at 274 nm (ASTM E1657-98) Maximum data rate 50 Hz (LAN)/20 Hz (Analog)/10 Hz (RS-232) Flow cell Not included (see Accessories / Spare parts) Time constants 0.00 / 0.02 / 0.05 / 0.1 / 0.2 / 0.5 / 1.0 / 2.0 s Integration time Automatic Detectors | 20 Communication Inputs Autozero, Start (IN), Error (either IN or OUT) Outputs Error (either OUT or IN) Analog inputs Wavelength 0 - 10 V Analog outputs 1 x ± 2.5 V scalable, 20 bit Control Front panel, Mobile Control, software, event control, analog, terminal protocol Interfaces LAN (RJ-45), RS-232 (SUB-D 9), multi-pin connector, analog (RCA cinch connector) Technical parameters GLP Lamp operating hours Display LED Ambient conditions Temperature range: 4 - 40 °C, 39.2 - 104 °F, Humidity: below 90 % noncondensing General Power supply External: input 100 - 240 V, output 24 V DC, 60 W Dimensions 121 x 129 x 187mm (W x H x D) Weight 1.5 kg Ordering details: Device ADA00 AZURA® Detector UVD 2.1S with deuterium lamp without flow cell, incl. test cell ADA05 AZURA® Detector UVD 2.1S Fiber Optics Version with deuterium lamp without flow cell Accessories A4061XB 10 mm path length, 10 µl, 1/16”, 300 bar, stainless steel, with heat exchanger one sided inlet and outlet, classical KNAUER flow cell A4045 3 mm path length, 2 µl, 1/16”, 30 bar, biocompatible, classical KNAUER flow cell A4042 3 mm path length, 2 µl, 1/16”, stainless steel, classical KNAUER flow cell A5193 Deuterium lamp, replacement, for S25 20, 10D, 40D, UVD 2.1S, UVD 2.1L, DAD 2.1L, MWD 2.1L 21 | Detectors The AZURA® RID 2.1L is a sensitive and competitively priced differential refractometer. It is suitable for detecting compounds with little or no UV activity such as alcohols, sugars, lipids or polymers. This instrument is designed for use in analytical HPLC (high performance liquid chromatography) as well as under certain conditions for GPC (gel permeation chromatography) applications. Key features The intelligently designed optical unit with advanced temperature control ensures high sensitivity, fast baseline stabilization, and excellent reproducibility. Furthermore, the long-life LED, • Temperature controlled highly pressure resistant flow cell, improved safety features and enhanced diagnostics func-optical unit tions guarantee easy handling and minimal maintenance. The wide linear dynamic range and • Long-life LED 10 ml/min maximum flow rate make the AZURA® RID 2.1L the perfect choice for most labora- • Pressure resistant flow cell tory tasks. • 55 years experience • Made in Germany Specifications Detection KNAUER offers various software control options: Detector type Refractive index detector www.knauer.net/softwarecontrol Version analytical (RID 2.1L) / preparative (RID 2.1L HighFlow) Light source Long-life LED For detector accessories Detection channels 1 see p. 42 Refractive index range 1.00 - 1.75 RIU Noise ± 2.5 nRIU (RID 2.1L) / ± 50 nRIU (RID 2.1L HighFlow) Drift 200 nRIU/h (RID 2.1L) / 2000 nRIU/h (RID 2.1L HighFlow) Linearity > 1000 µRIU (RID 2.1L) / > 4000 µRIU (RID 2.1L HighFlow) Flow cell 5 bar back pressure resistance Flow cell included Max. flow rate 10 ml/min (pure water) (RID 2.1L) / 100 ml/min (pure water) (RID 2.1L HighFlow) Flow cell volume 15 µl Wetted materials Stainless steel / fused silica / PTFE / PEEK Temperature control OFF, 30 - 55 °C (1 °C increment) Time constants 0.00 / 0.01 / 0.02 / 0.05 / 0.1 / 0.2 / 0.5 / 1.0 / 2.0 / 5.0 / 10.0 s Maximum data rate 100 Hz (LAN)/20 Hz (Analog) Autozero Full range Leak sensor Yes (internal and external leak management) Communication Inputs Error (IN), Start (IN), Autozero, Flush (IN) Outputs Event 1, Start (OUT), Error (OUT), + 5 V, 24 V Valve Analog outputs 1 x 0- 2.5 V scalable, 20 bit, offset adjustable Control Mobile Control, software, event control, analog, terminal protocol Interfaces 2 x LAN (RJ-45, dual IP-stack), USB (service only), multi-pin connector, analog (cinch connector) Technical parameters GLP Detailed report including operating hours, light source operating hours Display Mobile Control (optional) Ambient conditions Temperature range: 4 - 40 °C, 39.2 - 104 °F, Humidity: below 90 % noncondensing General Power supply 100 - 240 V, 50 - 60 Hz, 65 W Dimensions 361 x 158 x 523 mm (W x H x D) Weight 10.8 kg Other Optional accessories Mobile Control Ordering details: ADD31 AZURA® Detector RID 2.1L analytical refractive index detector with flow cell ADD38 AZURA® Detector RID 2.1L HighFlow preparative refractive index detector with flow cell and external pressure release valve Detectors | 22 The 4000 MiD is a miniaturized chip-based single quadrupole mass spectrometer suitable for a broad range of applications. An integrated oil-free pump system enables a small footprint and thereby the installation of this detector nearly everywhere. The easy to use concept with plug and play consumable results in low maintenance costs and a completely tool-less front end. Due to its small design, the nitrogen consumption of the 4000 MiD is reduced leading to low operating costs. With the MiDas automated sampling unit, the 4000 MiD is the ideal addition for preparative HPLC systems and mass-directed purification. With the integrated vacuum system and integrated electronics inside of one box the KNAUER 4000 MiD brings mass spectrometry to places where no other spectrometer can be deployed. Key features With a mass range of 50 to 800 m/z, the KNAUER 4000 MiD can be used for a broad variety of • Wide application range applications. In combination with the KNAUER MiDas, it is the ideal choice for preparative chro- • Compact matography and direct introduction methods. The mass spectrometer can be controlled with ClarityChrom® 7.4.1 and higher, as well as with PurityChrom® 5.09.069 and higher allowing it to be integrated into KNAUER LC systems. KNAUER offers various software control options: Specifications www.knauer.net/softwarecontrol Detection For detector accessories see p. 42 Detector type Mass spectrometer MS Detection MS type Single Quadrupole Ionization modes ESI (positive and negative) Mass range 50 m/z to 800 m/z Mass sensitivity Mass resolution m/z 0.7 (FWHM) Mass accuracy m/z ± 0.3 (Full scan) Gas requirements Gas Nitrogen Gas flow rate 2.5 l/min Gas inlet pressure 2-6 bar General Dimensions 550 x 350 x 250 mm (L x W x H) Weight 32 kg (4000 MiD only) Ordering details: Device A66900 Chip-based miniaturized mass spectrometer with flow split module MiDas Accessories A66921 Solaris Air Compressor for Solaris nitrogen gas generator A66920 Nitrogen gas generator Solaris without air compressor, 10 l/min A0000MSEU Installation and instruction in Europe, 2 days, including flight and hotel. Training in MS basics not included. A0000MSIN Installation and instruction International, 2 days, including flight and hotel. Training in MS basics not included. 23 | Detectors The fluorescence detector RF-20A provides world-class sensitivity, excellent maintainability and diverse validation / support functions. It supports a wide range of applications in the wavelength range of 200 to 650 nm from conventional analysis to high-performance analysis. With a signal-to-noise ratio of 1200 for the water-Raman band, the fluorescence detector is well suited for trace analysis. The xenon lamp and flow cell are directly accessible on the device, thus allowing a quick and easy handling and maintenance of the device by the user, thereby minimizing downtime. The lamp life is 2000 hours. When replacing the xenon lamp, no adjustment is required. Key features Specifications (for RF-20A) • Pressure resistant flow cell Detection KNAUER offers various Detector type Fluorescence detector software control options: Detection channels 1 www.knauer.net/softwarecontrol Number of signals 1 Light source xenon lamp For detector accessories see p. 42 Wavelength range 200 - 650 nm Spectral bandwidth 20 nm Wavelength accuracy ± 2 nm Wavelength precision ± 0.2 nm Indicates the precision performance when the power is turned ON in the single wavelength mode and the wavelength is changed. Sensitivity can be set at three levels: HIGH (x 1), MED (x 32), LOW (x 1024) Wetted materials SUS316L, PTFE (fluorocarbon polymers), quartz Flow cell volume 12 µl Time constants 11 levels can be selected, equivalent to “no filter”, 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 3.0, 6.0, 8.0 and 10.0 sec-onds Autozero auto zero function, basline shift function Communication Gain can be set at three levels: x 1, x 4, x 16 Technical parameters Ambient conditions operating temperature: 4 to 35 °C, relative humidity: 20 to 85 % (no condensation) General Power supply AC220–240 V, 400 VA, 50/60 Hz Dimensions 260 x 210 x 420 mm (W x H x D) Weight 16 kg Ordering details: Device A59200 Fluorescence detector RF-20 A 200 - 650 nm incl. accessories and flow cell A59201 Fluorescence detector RF-20 Axs 200 - 750 nm incl. accessories and flow cell A59203 Fluorescence detector RF-20 A with photomultiplier from 200 - 900 nm incl. accessories and flow cell A59204 Fluorescence detector RF-20 Axs with photomultiplier from 200 - 900 nm incl. accessories and flow cell Accessories A59210 Xenon lamp for RF-20A/Axs fluorescence detector A59211 Flow cell for Semi micro LC cell capacity 3 µl, supports temperature control (RF-20Axs only) A59212 Inert flow cell for RF-20A/Xs, cell capacity 12 µl, contact materials: PEEK, PTFE A59300 CBM-20A Interface CE/IVD PC Interface (request software control option via KNAUER) A1783-3 Shimadzu LC Driver for Chromeleon 7.2 Shimadzu CBM-20A required Detectors | 24 Evaporative Light Scattering Detection (ELSD) is a universal modern technology with which every analyte that is less volatile than the mobile phase can be detected. Using the unique Low Temperature technology, this detector allows the achievement very high sensitivity. The technology is gradient compatible and is independent of the absorption characteristics of the eluents. Compounds can be universally measured with this detector (carbohydrates, proteins, peptides, polymers, lipids, steroids, etc.), regardless of their fluorescence, absorption or refractive-index characteristics. Comprehensive SOP protocols for GLP conformity and validation procedures are available. Specifications (for Sedex 85 LT) Key features Detection • Attractively priced Detector type Light scattering detector • Wide application range Detection channels 1 • Large choice of nebulizers Light source selected high effciency blue LED (470 nm), elapsed-time counter Sensitivity < 1 ng caffein (LOD) KNAUER offers various Maximum data rate Analog: 100 Hz/Digital: 30 Hz software control options: www.knauer.net/softwarecontrol Gas requirements For detector accessories Gas nitrogen preferred see p. 42 Gas flow rate < 3 l/min Gas inlet pressure 3.5 bar HPLC flow rate standard HPLC with 4 nebulizers: 0.2 - 2.5 ml/minultra high performance LC with 1 nebulizer Maintenance easily accessible from the front for cleaning Heated zone Temperature range ambient to 100 °C Communication Gain 1 to 12 - factor 211 (2048) Filter moving average (0 – 10 s) Analog outputs 0 – 1 V Analog control input contact closure, TTL for ready, autozero, power down Control RS-232 Power-down methods shut-off: gas, LED, heating and/or PMT cleaning mode Technical parameters Display LCD and keypad General Power supply 230 V/50 Hz, 1.7 A - 115 V/60 Hz, 1.8 A Dimensions 250 mm x 480 mm x 550 mm (W x H x D) Weight 16 kg Ordering details: Device A0754-1 Sensitive Light scattering detector ELSD 85LT for univ. detection 0.2 - 2.5 ml/min, 100 Hz including accessories A0754-3 High sensitive ELSD 90LT for univ. detection for HPLC and ultrafast HPLC, low temperature technology, supports high data rates A0754-5 Preparative Light scattering detector ELSD SEDEX LC for univ. detection 200 µl/min - 2 ml/min A0754-6 Ultra high sensitive light scattering detector ELSD SEDEX 100LT for univ. detection 200 µl/min - 2 ml/min 100 Hz including accessories, SAGA Accessories A2618-01 OpenLAB® CDS EZChrom Edition drivers for 80LT, 85LT, 90LT, 100LT and LC from Sedere A1783-4 Sedex Driver for Chromeleon 7.2; For Sedex 85LT / 90LT; Instrument Controler Class 3 necessary A1783-5 Sedex Driver for Chromeleon 7.2; For Sedex FP / LC / 100LT; Instrument Controler Class 3 necessary 25 | Detectors With its measurement frequency up to 100 Hz, this electrochemical detector is specially designed for super-fast highly sensitive and selective measurement of oxidisable and reducible substances in (U)HPLC. The AZURA® ECD 2.1 comprises of a thermostat-controlled Faraday’s cage, accom-modating column and flow cell. The AZURA® ECD 2.1 unites the three operating modes DC, Pulse and Scan in one instrument. The DC mode covers about 90% of all applications. The pulse mode is important for PAD (Pulsed Amperometric Detection) of e.g. carbohydrates. The scan mode is used to obtain a voltammogram in method optimization. A digital low-pass filter provides an excellent signal-to-noise ratio. For highest sensitivity, the SenCell flow cell is recommended. The correct flow cell can be chosen from a broad variety of flow cells after our advice. Specifications Key features • Compact • Thermostat included Puls mode Measuring range 10 pA-200 μA in steps of 1, 2 and 5 Filter (cut off) Advanced Digital Filter, 0.5 -0.001 Hz in steps of 1, 2 and 5 KNAUER offers various Pulse times t1: 100-2000 ms; t2: 0-2000 ms; t3: 0-2000 ms in steps of 10 ms software control options: Data recording 20, 40, 60, 80 and 100 ms www.knauer.net/softwarecontrol For detector accessories DC mode see p. 42 Measuring range 10 pA-200 μA in steps of 1, 2 and 5 Filter (cut off) Advanced Digital Filter, 0.5 -0.001 Hz in steps of 1, 2 and 5 Noise < 2 pA with dummy cell Scan mode Measuring range 10 pA-200 μA in steps of 1, 2 and 5 Scan speed 1-50 mV/s in steps of 1, 2 and 5 Scan cycle half, complete, continuous Detection Detector type Electrochemical detector Version DC, pulse, scan operating modes Detection channels 1 Working potential -2.0 V to +2.0 V Maximum data rate 10 Hz Autozero triggered via key, TTL, RS-232 Heated zone Thermostatting oven included Temperature range from 7°C above ambient temperature to 45°C Communication Control Parametric control and data-acquisition via LAN port (USB service port) Ordering details: Device A1651 Electrochemical detector AZURA® ECD 2.1without flow cell Flow cells Art. no. Flow cell type Typical applications A1652 SenCell 2 mm GC Salt-Bridge DC mode for Catecholamines A1652-1 FlexCell Au HyREF 3-Step PAD for Carbohydrates A1652-3 SenCell Au HyREF DC mode for Phenols, Polyphenols, Bisphenol A A1652-2 SenCell GC HyREF 4-Step PAD for Carbohydrates Detectors | 26 The CDD-10AVP is a highly senstitiv conductivity detector applicable to ion chromatograph or organic acid analysis. Low noise, low drift and wide dynamic range assure proven performance of the CDD-10AVP detector. A special feature is the VP key for validation. Flow cell 0.25 µl included. Specifications Detection Detector type Conductivity detector KNAUER offers various Detection channels 1 software control options: Measurement range 0.01 - 52000 µS/cm www.knauer.net/softwarecontrol Noise < 4 nS/cm Drift < 25 nS/cm per hour For detector accessories Flow cell volume 0.25 µl see p. 42 Time constants 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 3.0, 6.0, 8.0, 10.0 s Communication Outputs 10 mV recorder terminal, integrator Ordering details: Device A1252-1 Conductivity detector CDD-10 Avp with flow cell 0.25 µl Accessories AZB00XA AZURA® Interface Box IFU 2.1 LAN, A/D converter, 4 channels 27 | Detectors The AZURA® CM 2.1S is a reliable conductivity monitor which is utilized in FPLC to follow salt gradients. Optionally pH values can be measured. Wetted parts are completely biocompatible. The contactless measurements of conductivity reduces the risk of carryover to a minimum. The flow cells covers a flow rate up to 100 ml/min and is delivered with the CM 2.1S. Please order the pH measuring kit in addition to the conductivity monitor. Note that the flow cell for up to 10 ml/min generates a certain back pressure (approximately 1 bar at 1 ml/min). Choose the device with the 100 ml/min flow cell for minimal back pressure. KNAUER offers various Specifications software control options: www.knauer.net/softwarecontrol Flow cell features Flow cell features by device Biocompatible Yes Device order number ADG30GC ADG30GD Capillary connection 1/16” Flow cell volume 30 µl 300 µl Wetted materials PEEK Max. flow rate 10 ml/min 5 - 100 ml/min Maximum pressure 160 bar 100 bar Back pressure 1 bar at 1ml/min - Detection Detector type Conductivity monitor Measurement accuracy Conductivity accuracy: < 5 % full scale end value pH accuracy: +/-0.5 pH in temperature range of 4 – 25°C Temperature sensor accuracy: ± 1.0 °C Measurement precision Conductvity precision: measured in range 0.1 – 300 mS/cm: < 2% of end value or ≤ 5 mS/cm of higher values pH precision: +/-0.2 pH in temperature range 4 – 25°C Measurement range 0.1 – 999 mS/cm pH measurement 2 – 12 Maximum data rate 5 Hz Supported electrodes all pH electrodes with BNC connector Communication Analog outputs 2 channels (Conductivity value and pH Value) DAC 18 bit Digital outputs LAN; RS-232 Technical parameters GLP electronic Serial No. Ambient conditions operating temperature: 4 to 35 °C, relative humidity: 45 to 85 % Display LCD, 2 x 8 characters General Power supply 100 – 240 V, 50 – 60 Hz, max. 20W Dimensions 121 x 129 x 187 mm (W x H x D) Ordering details: Device ADG30GC AZURA® CM 2.1S with flow cell - up to 10 ml/min - conductivity monitor with optional pH measurement ADG30GD AZURA® CM 2.1S with flow cell - up to 100 ml/min - conductivity monitor with optional pH measurement Accessories A4156 Flow cell CM 2.1S for flow rates up to 10 ml/min A4157 Flow cell CM 2.1S for flow rates up to 100 ml/min A70091 pH measuring kit for conductivity monitor CM 2.1S for flow rates up to 80 ml/min, delay volume 80 µl, max. pressure 5 bar A5813 Flow splitter for CM 2.1S when used with flowrates over 100 ml/min A9854-3 Mounting bracket AZURA® L for AZURA® Valve Unifier VU 4.1(both-sided) or AZURA® UVD 2.1S and AZURA® CM 2.1S (left-sided on AZURA® L) Detectors | 28 The Foxy® fraction collector can be adapted to a broad spectrum of applications. Fractions can be collected into small tubes, standard tube sizes, and bottles. For essentially unlimited volumes, funnel racks can direct fluids to any collection vessel or downstream process. Specifications Fraction collection Brand Foxy R1 / Foxy R2 KNAUER offers various Fractionation modes drop counting, time intervals, volume intervals, level, slope software control options: Max. flow rate 25 ml/min or 125 ml/min www.knauer.net/softwarecontrol Fraction capacity see list of racks in accessories (next page) Diverter valve yes (R1) / no (R2) For purification accessories Wetted materials valve: PEEK and perfluoroelastomer (FFKM), see p. 48 supplied ferrules: ETFE, supplied valve tubing: PTFE, supplied drain tubing: viny Fractionation control operator: front panel control via touch screen LCDintegrated systems: direct communication via Ethernet (TCP/IP) and RS-232 serial communications Maximum test tube height 160 mm(R1) / 1/8” - 196 mm; 1/4” - 180 mm (R2) RFID rack recognition no (R1) / yes (R2) Number of racks 1(R1) / 2 (R2) Capillary connection 1/16” : 25 ml/min 1/8” : 125 ml/min 1/4” : 1000 ml/min Communication Control LAN, RS-232 Technical parameters Conformity CE, CSA Display touch screen LCD displays Ambient conditions 0–40 °C, 32–104 °F General Power supply 100–240 VAC, 50–60 Hz, max. 1 A Dimensions R1: 311 x 330 x 355 mm (W x D x H) R2 1/8”: 311 x 533 x 378 mm (W x D x H) R2 1/4”: 311 x 533 x 394 mm (W x D x H) Weight R1: 7.1 kg R2 1/8”: 10.3 kg R2 1/4”: 10.4 kg Fraction collectors 29 | Fraction collectors Ordering details: Device A59100 Fraction collector Foxy® R1 for 1/16“ or 1/8“ tubing A59102 Fraction collector Foxy® R2 for 1/16“ or 1/8“ tubing A591021 Fraction collector Foxy® R2 for 1/4“ tubing Accessories A59122 Cooling option for Foxy® R1 with cooling hood, cooling plate and accessories A59117 Cooling rack for 144 tubes 1.5 ml for Foxy® R1 * A59118 Cooling rack for 72 Falcons 15 ml for Foxy® R1 * A59119 Cooling rack for 96-Well Microplates for Foxy® R1 * A59105 Rack for 100 vials 16 mm/max. 20 ml for Foxy® R1/R2 A59104 Rack for 144 vials 13 mm/max. 9 ml for Foxy® R1/R2 A59111 Rack for 2 microwell plates 96 well for Foxy® R1/R2 A59114 Rack for 2 x 9 bottles 480 ml for Foxy® R2 (not suitable for Foxy® R1, bottles too tall) A59110 Rack for 36 Falcon 50 ml for Foxy® R1/R2 A59108 Rack for 36 vials 25 mm/max. 70 ml for Foxy® R1/R2 A59107 Rack for 60 tubes 1.5 ml for Foxy® R1/R2 A59106 Rack for 72 Falcons 15 ml for Foxy® R1/R2 A591091 Rack with 26 funnels with vinyl tubing for Foxy R2, up to 1000 ml/min A59109 Rack with 36 funnels with vinyl tubing for Foxy® R1/R2 A591092 Scintillation rack for 36 vials 28 mm for Foxy® R1/R2 A70055 Thermostatting unit -20° to 40°C A70050 Thermostatting unit -40° to 200°C * for Foxy R1 with cooling option Fraction collectors | 30 The LABOCOL Vario-4000 fraction collectors are characterized by their high robustness and optimal ratio of dimensions/benefit. The user is not limited to given rack types. The rack layout can be designed according to individual needs. Freely define the number of fraction vessels and their position. The wide application area make the Vario-4000 series ideal for use in research and development as well as in production. The Vario-4000 models differ in the base area and the flow rate range. Specifications Fraction collection Brand LABOCOL Vario-4000 / Plus KNAUER offers various Max. flow rate 100 ml/min for 1/16”; 500 ml/min for 1/8” software control options: Fraction capacity consider list of racks in accessories below www.knauer.net/softwarecontrol Wetted materials PEEK and PTFE Number of racks 3 (Vario-4000) / 5 (Vario-4000 Plus) For purification accessories Capillary connection 1/16” : 100 ml/min see p. 48 1/8” : 500 ml/min 1/4” : 1000 ml/min Communication Control LAN, RS-232 Technical parameters Ambient conditions 0–40 °C, 32–104 °F General Power supply 100–240 VAC, 50–60 Hz, max. 2.5 A Dimensions Vario-4000 30 x 50 cm (WxD) Max. floor space Vario-4000 Plus 46 x 50 cm (WxD) Vario-4000 24 x 41 cm (WxD) min. H *: 52 cm Vario-4000 Plus 40 x 41 cm (WxD) max. H *: 67 cm Weight 8 kg (Vario-4000) / 10 kg (Vario-4000 Plus) * with touchpanel Ordering details: Device A591023 Fraction collector LABOCOL Vario-4000 Plus, for 1/16“ or 1/8“ tubing A591026 Fraction collector LABOCOL Vario-4000 Plus, for 1/4“ tubing A591022 Fraction collector LABOCOL Vario-4000, for 1/16“ or 1/8“ tubing A591024 Fraction collector LABOCOL Vario-4000, for 1/4“ tubing Accessories A59134 Rack for 24 Falcon® tubes of 50 ml for LABOCOL Vario-4000/Vario-4000 Plus A59131 Rack micro for 125 tubes 10.5 mm/max. 9 ml for LABOCOL Vario-4000/Vario-4000 Plus A59132 Rack prep for 20 tubes 36 mm/max. 140 or 240 ml for LABOCOL Vario-4000/Vario-4000 Plus A59133 Rack semiprep for 39 tubes 26 mm/max 80 ml for LABOCOL Vario-4000/Vario-4000 Plus A59130 Rack standard for 80 tubes 18 mm/max. 36 ml for LABOCOL Vario-4000/Vario-4000 Plus A591029 Touchpanel for LABOCOL Vario-4000/Vario-4000 Plus 31 | Fraction collectors Dissolved gases in the solvent can cause bubbles in the flow path of pumps and detectors. Reliable chromatographic separation therefore requires degassing of the solvent. The small analytical 2-channel degasser DG 2.1S is equipped with two degassing chambers and can thus degas two solvents simultaneously. Specifications Degasser module Degasser channels 2 KNAUER offers various Max. flow rate/channel 10 ml/min software control options: Recommended flow rate/ 2.8 ml/min www.knauer.net/softwarecontrol channel Degassing method Gas permeation through a fluoropolymere membrane Degassing chamber volume 285 µl Solvent applicability universal, except hydrochloric acid, halogenated hydrocarbons, hexafluoro isopropanol (HFIP), tetrahydro-furan, hexane, heptane Wetted materials PTFE, PPS, PEEK, Systec AF TM Maximum pressure stability 70 psi Technical parameters Display 1 LED Ambient conditions temperature range: 4 - 40 °C, 39.2 - 104 °F air humidity: below 90%, non-condensing General Power supply 85 - 265 V, 50 - 60 Hz, 20 W Dimensions 121 x 138 x 190 mm (W x H x D) Weight 2.3 kg Feature overview Order no. Degasser type Channels Max. flow rate Chamber volume AZE02 analytical 2 10 ml/min (recommended 2 ml/min) 285 µl per channel AZE03-1 analytical 4 10 ml/min (recommended 2 ml/min) 285 µl per channel A5335 analytical, for GPC 2 10 ml/min (recommended 3 ml/min) 480 µl per channel A5328 semi-preparative 2 30 ml/min (recommended 15 ml/min) 5.3 µl per channel AZE02-1 preparative 2 200 ml/min (recommended 75 ml/min) 23 ml per channel AZE03 preparative 4 200 ml/min (recommended 75 ml/min) 23 ml per channel Ordering details: AZE02 Biocompatible 2 channel degasser AZE03-1 Analytical 2 channel GPC Degasser A5335 Semi-preparative 2 channel Degasser AZE03-1 Analytical 4 channel Degasser, biocompatible AZE02-1 Preparative 2 channel degasser, biocompatible AZE03 Preparative 4 channel degasser, biocompatible Degassers Degassers | 32 The valve drive AZURA® Valve Unifier VU 4.1 enables automatic valve switching. Due to its low switching time, the flow path is blocked only for a very short time, and pressure peaks are reduced to a minimum. Valves are identified via RFID technology, which guarantees an easy valve exchange of KNAUER valves with RFID technology. This RFID technology allows to easily check GLP data. and this way to simplify maintenance such as the exchange of a rotor seal. The display enables user-friendly standalone operation. In addition, the valve drive can be operated with software as well with an optional touch display (Mobile Control), via LAN or analog input/ output, by which it can be integrated into nearly every LC system. Specifications Communication Key features Interfaces LAN, display, terminal strip • One valve drive for all valves • Ultra fast switching cycle Control Display, software, event control • Easy maintenance Inputs Binary control; Home, Backward/Inject, Forward/Load, Start IN • Compact Outputs Trigger out, Event • Multiple interfaces and drivers available General Power supply External DC 24V, 65 W Dimensions 80 x 123 x 192 mm (W x H x D) Weight 1.9 kg KNAUER offers various Ambient conditions Temperature range: 4–40 °C; 39.2–104 °F software control options: below 90 % humidity (non condensing) www.knauer.net/softwarecontrol For valve accessories see p. 45 AVS34CE AVN94CE AVR38AC Valve drive VU 4.1 (AWA01) AVC38AC with 6 port 2-position valve AVS62CE (AVC28AC) Ordering details: Valve drive Device Order number VU 4.1 valve drive for V 4.1 valves AWA01 Mounting bracket Device Order number Mounting bracket to mount VU 4.1 to AZURA L device A9854-3 33 | Valves Valves Valves for valve drive VU 4.1 Manual valves* *the mounting bracket A9853 is required to mount the manual valves to an AZURA L device Ports Stator material Rotor material Capillary connection Max. pressure Bore size Order number [bar] [mm] 6 SST DLC1 PEEK 1/16" (UNF 10-32) 500 0.75 AVJ26AE 6 SST DLC1 Vespel 1/16" (UNF 10-32) 1200 0.3 AVI28AC 6 PEEK PEEK 1/16" (UNF 10-32) 240 0.75 AVG24CE 6 SST DLC1 PEEK 1/8" (UNF 1/4-28 coned) 300 1.5 AVK25AE 6 PEEK PEEK 1/8" (UNF 1/4-28 coned) 50 2 AVL22CE 8 SST DLC1 PEEK 1/16" (UNF 10-32) 500 0.75 AVJ36AE 8 SST DLC1 Vespel 1/16" (UNF 10-32) 1200 0.3 AVI38AC 2-position valves Ports Stator material Rotor material Capillary connection Max. pressure Bore size Order number [bar] [mm] 6 SST DLC1 PEEK 1/16" (UNF 10-32) 500 0.75 AVD26AE 6 SST DLC1 Vespel 1/16" (UNF 10-32) 1200 0.3 AVC28AC 6 PEEK PEEK 1/16" (UNF 10-32) 240 0.75 AVD24CE 6 SST DLC1 PEEK 1/8" (UNF 1/4-28 coned) 300 1.5 AVE25AE 6 PEEK PEEK 1/8" (UNF 1/4-28 coned) 50 2 AVF22CE 8 SST DLC1 PEEK 1/16" (UNF 10-32) 500 0.75 AVD36AE 8 SST DLC1 Vespel 1/16" (UNF 10-32) 1200 0.3 AVC38AC Multiposition valves Ports Stator material Rotor material Capillary connection Max. pressure Bore size Order number [bar] [mm] 6 SST DLC1 Vespel 1/16" (UNF 10-32) 1200 0.3 AVR28AC 6 SST DLC1 PEEK 1/16" (UNF 10-32) 500 0.75 AVS26AE 6 SST DLC1 PEEK 1/8" (UNF 1/4-28 coned) 300 1.5 AVT25AE 8 SST DLC1 Vespel 1/16" (UNF 10-32) 1200 0.3 AVR38AC 8 SST DLC1 PEEK 1/16" (UNF 10-32) 500 0.75 AVS36AE 8 SST DLC1 PEEK 1/16" (UNF 10-32) 300 0.75 AVS35AE 8 PEEK PEEK 1/16" (UNF 10-32) 240 0.75 AVS34CE 8 SST DLC1 PEEK 1/8" (UNF 1/4-28 coned) 200 1.5 AVU34AE 8 PEEK PEEK 1/8" (UNF 1/4-28 coned) 50 2 AVU32CE 12 SST DLC1 PEEK 1/8" (UNF 1/4-28 coned) 50 1.5 AVT52AE 16 SST DLC1 PEEK 1/16" (UNF 10-32) 500 0.6 AVQ66AE 16 PEEK PEEK 1/16" (UNF 10-32) 50 0.75 AVS62CE Special purpose valves* *for detailed information please check our website: www.knauer.net/valves Valves Capillary Max. pressure Bore size Order number connection [bar] [mm] Column selection valve, biocompatible 1/16" 50 0.4 AVZ52CE Allows switching of up to 5 columns incl. bypass and (UNF 10-32) reverse flow option. Multi-injection valve, biocompatible 1/16" 240 0.75 AVN94CE Allows manual and automated sample loading as well (UNF 10-32) as direct injection. Multi-injection valve, stainless steel 1/16" 500 0.75 AVN96AE Allows manual and automated sample loading as well (UNF 10-32) as direct injection. ----- 1 stainless steel coated with diamond-like carbon Valves | 34 KNAUER is one of the pioneers in the field of osmometry and known for its reliable and user friendly instruments for many decades. Our newest freezing point osmometer K-7400S allows the easy and fast determination of the osmolality of various aqueous solutions. Also, the freezing point depression of the samples can be read. The proven technology of freezing point determination in combination with the robust and intelligent design of the device allows reproducible measurements. The instrument is equipped with a peltier cooler and an integrated microprocessor controlling the automated measurement. The freezing point osmometer is a standalone device that optionally can be equipped with a printer. Furthermore, the device can be controlled via the EuroOsmo 7400 software. The software automatically plots the temperature curve for each measurement and Key features calibration and allows saving of the measured values. In addition, the data can optionally be exported into various file formats for archival storage. • Made in Germany • 55 years experience The K-7400S freezing point osmometer meets the requirements of the European Pharmacopoeia • fast measurements for osmolality (2.2.35, 01/2012). Specifications Technical parameters Sample volume 50 - 150 µl Osmolality range 0 - 2000 mOsmol/kg Resolution osmolality: integer value without decimal part, e.g. 850 mOsmol temperature: value with three digits, e.g. -1.576 °C Test time ~ 2 min Precision SD ≤ 4 mOsmol/kg [0 — 400 mOsmol/kg] RSD ≤ 1 % [400 — 2000 mOsmol/kg] Linearity ± 1 % [0 -1500 mOsmol/kg] ± 1.5 % [0 - 2000 mOsmol/kg] Calibration Two-point calibration (0 mOsmol/kg and one freely selectable osmolality) optional: Three-point calibration (0 Osmol/kg and two free selectable osmolalities) General Power supply 100 – 240 V, 50 – 60 Hz, 70 W Dimensions 160 x 182 x 340 mm (W xH x D) Weight 5.3 kg Ambient conditions Temperature range: 10–35 °C Rel. humidity: 20–80 % (non-condensing) Communication Interfaces RS-232 port Control Keypad (LED display, 2 rows with 24 characters) optional: EuroOsmo7400 Software Ordering details: Device A0006AC Osmometer for the determination of osmolality or freezing point of aqueous solutions Accessories A0840-2 Measuring head for plastic sample tubes; compatible with the K-7400 and the K-7400S Semi-Micro Osmometer A3705 EuroOsmo 7400 - software for K-7400 and K-7400S osmometers A3711 Plain paper printer for freezing point osmometer K-7400 and K-7400S A13270 Barcode Scanner with USB cable, for EuroOsmo 7400 A0011XB Upgrade Kit for K-7400 required to use measuring head for sample tubes (A0840-2) For more osmometry accessories and standards see p. 47 Osmometers 35 | Osmometers Accessories Eluent trays & bottles 250 ml Bottle for Piston Back Flushing A2056 AZURA® Eluent tray E 2.1L for AZURA® devices with a capacity of 6 x 1 l bottles or AZC00 4 x 2.5 l bottles or 2 x 5 l bottles, (delivery without bottles) Eluent supply bottle 1000 ml, GL45 thread, square, Clear glass, with screw cap with A5325 2 holes for tubing AZC00 Eluent supply bottle plastic 2 L incl. cap and tubing for IC and ECD systems A70038 GL45 thread, round, 2000ml, Clear glass, without screw cap A59158-1 Set of eluent supply bottles, 2 x 1 L bottles and 1 x 250 ml bottle for piston A5324-4 backflushing, includes closed screw caps Set of eluent supply bottles, 2 x 1 L bottles, includes screw caps with holes for eluent A5324-1 tubing Set of eluent supply bottles, 3 x 2.5 L brown glass bottles (borosilicate glass) with A70037 special round bottom for minimal eluent remainder, for preparative HPLC/FPLC, A70037 A5324 A2056 includes screw-type cap A5324-1 A5324-2 Set of eluent supply bottles, 4 x 1 L bottles for Eluents and 1 x 250 ml bottle for A5324-3 piston backflushing, includes closed screw caps Set of eluent supply bottles, 4 x 1 L bottles for Eluents and 1 x 250 ml bottle for A5324-2 piston backflushing, includes screw caps with holes for eluent tubing Set of eluent supply bottles, 4 x 1 L bottles, includes screw caps with holes for eluent A5324 tubing Mass flow controllers* Mini CORI-Flow (M13) Mass flow controller incl. mounting block, A5390 Flow: 1 - 50 ml/min, stainless steel 316 Mini CORI-Flow (M14) Mass flow controller incl. mounting block, A5391 Flow: 2 - 833 ml/min, stainless steel 316 Mini CORI-Flow (M13) Mass flow controller incl. mounting block, A5393 Flow: 1 - 50 ml/min, stainless steel 316, Profibus Mini CORI-Flow (M12) Mass flow controller incl. mounting block, A5394 Flow: 0,03 – 3,3 ml/min, stainless steel 316 Mini CORI-Flow (M13) Mass flow controller incl. mounting block, A5395 Flow: 1 - 50 ml/min, Hastelloy-C22 HI-TEC Bright display for Mini CORI-Flow mass flow controller A5396 (display, setpoint and counter) *analog and bus versions on request Static mixers AZURA® HPLC mixer up to 100 MPa, 50 µl mixing volume, stainless steel AZZ00MB AZURA® HPLC mixer up to 100 MPa, 100 µl mixing volume, stainless steel AZZ00MC AZZ00MB AZURA® HPLC mixer up to 100 MPa, 200 µl mixing volume, stainless steel AZZ00MD AZZ00MC AZURA® HPLC mixer up to 40 MPa, 250 µl mixing volume, PEEK (biocompatible) AZZ10ME AZZ00MD HyperShear Static Mixer, (1.5mL), high flow series, 414 bar max. pressure, A5830 stainless steel and PEEK Mounting bracket AZURA® L for Hypershear mixing chambers A9853-8 AZZ10ME A5830 Pump accessories | 36 Dynamic mixers Dynamic mixing chamber (250 V), stainless steel, analytical, 1/16“, up to 420 bar, A0285 1740 µl mixing volume Dynamic mixing chamber (115 V), stainless steel, analytical, 1/16“, up to 420 bar, A02851 1740 µl mixing volume Dynamic mixing chamber (250 V), stainless steel, preparative, 1/8“, up to 250 bar, A0581 5.9 ml mixing volume Dynamic mixing chamber (115 V), stainless steel, preparative, 1/8“, up to 250 bar, A05811 5.9 ml mixing volume Dynamic mixing chamber (250 V), titanium, analytical, 1/16“, up to 420 bar, A0275 1740 µl mixing volume Dynamic mixing chamber (115 V), titanium, analytical, 1/16“, up to 420 bar, A02751 1740 µl mixing volume Dynamic mixing chamber (250 V), titanium, preparative, 1/8“, up to 250 bar, A70581 5.9 ml mixing volume Dynamic mixing chamber (115 V), titanium, preparative, 1/8“, up to 250 bar, A705811 5.9 ml mixing volume Mixing chamber extension unit for A70581/ A705811, 1 intermediate section, A2515 titanium, 4.8 ml, 3 screws 6 x 60 mm Solvent filters & inlet tubing Mobile Phase Filter, stainless steel, 2 µm, 1/8” pipe OD, A3373 A3374 A3364 suitable for all analytical HPLC systems A3375 Mobile Phase Filter, stainless steel, 20 μm, for 1/8” OD, compatible with the AZURA® A3374 Tubing Kit (A9650), suitable for all analytical and semi preparative HPLC systems, max. flow rate 100 ml/min Mobile Phase Filter, stainless steel, 10 μm, for 1/8” OD,compatible with the AZURA® A3375 Tubing Kit (A9650), suitable for all analytical HPLC systems, max. flow rate 10 ml/min A9650 Mobile Phase Filter, Biocompatible PE, 20 µm, 1/8“ pipe OD, A3364 suitable for all FPLC systems, max. flow rate 500 ml/min AZURA® Tubing kit with cap and solvent filter (A3375, stainless steel, 10 µm), A9650 suitable for all analytical HPLC systems AZURA® Tubing Kit bio with cap and insert, solvent filter inlet and fittings, 1set A96507 Filter Cartridge for pump P 6.1L, Titanium frit, 2 µm pore size. A9661 A96507 50 ml/min maximum flow, High capacity filter, 60 µl volume, 3 pcs. Filter Cartridge for pump P 6.1L, Stainless steel frit, 2 µm pore size. A96601 10 ml/min maximum flow, Volume optimized filter, 20 µl volume, 3 pcs. Pulse dampers KNAUER Pulse Damper, high volume, stainless steel AZZ00NB KNAUER Pulse Damper, low volume, stainless steel AZZ00NA Mounting Bracket KNAUER Pulse Damper FZZ2 37 | Pump accessories Pump head inlet fittings Pump head inlet for AZURA® Pump P 2.1L, BlueShadow 80P, 1/4” (NPT), A9861 stainless steel, max. flow 250 ml/min Pump head inlet for AZURA® Pump P 2.1L, Set, 1/2”-20 UNF, PEEK with CTFE (Kel-F) A9868 A9861 adapter, including tubing 1/4“ PTFE Inlet bushing for prep pump heads, adapter to 3/8” tube stub A98611 Inlet bushing for binary LPG prep pump heads, LPG inlet to 3/8” tube stub A98612 Inlet bushing for LPG prep pump heads, LPG ternary inlet to 3/8” tube stub A98613 Male connector to connect a 1/4’’ OD capillary to a 1/4’’ male NPT adapter A58267 (material: stainless steel) for A9861 A9868 Male connector to connect a 4 mm OD capillary to a 1/4’’ male NPT adapter A58268 (material: stainless steel) for A9861 Male connector to connect a 1/8’’ OD capillary to a 1/4’’ male NPT adapter A58269 (material: stainless steel) for A9861 Inlet-bushing kit for P 2.1S, P 4.1S, P6.1L, 40P and S1050 pumps A58202 for pump heads 10 ml (1/8” capillaries) A58267 A98611 Inlet-bushing kit for P 2.1S, P 4.1S, P6.1L, 40P and S1050 pumps A58203 A58268 A98612 for pump heads 10 ml (1/16” capillaries) A58269 A98613 Inlet-bushing kit for P 2.1S, P 4.1S, P6.1L, 40P and S1050 pumps A58204 for pump heads 50 ml (1/8” capillaries) Inlet-bushing kit for P 2.1S, P 4.1S, P6.1L, 40P and S1050 pumps A58205 for pump heads 50 ml (1/16” capillaries) Solvent Filter 1/4”-PTFE Tubing up to 250 ml/min A58207 A58203 Pump head outlet fittings Outlet-bushing kit 1/8” tube stub for S1800, 80P and P 2.1L pumps A5822 Adapter to connect a capillary with 1/16” OD (thread: 10-32 UNF) to AZURA® Pump A7200 P2.1L or BlueShadow Pump 80P outlet (1/8”, M8x1 thread), material: stainless steel A5822 Replacement pump heads for analytical AZURA® pumps Pump head 5 ml, stainless steel (1000 bar) AHA60 Pump head 10 ml, stainless steel AHB40 Pump head, 10 ml, stainless steel, for aqueous solutions AHB40FA Pump head 10 ml, stainless steel, for high temperature dosing applications AHB40CA Pump head 10 ml, stainless steel, for normal phase applications AHB40BA Pump head 10 ml, ceramic AHB32 Pump head 10 ml, ceramic with Ti-bushings AHB32DA AHB32 Pump head 10 ml, ceramic, Titanium bushings, for aqueous solutions AHB32GA Pump head 10 ml, Hastelloy-C, for corrosive chemicals AHB43 Pump head 50 ml, stainless steel AHC20 Pump head 50 ml, stainless steel, for aqueous solutions AHC20FA Pump head 50 ml, stainless steel, for normal phase applications AHC20BA Pump head 50 ml, stainless steel, for high temperature dosing applications AHC20CA Pump head 50 ml, ceramic AHC22 Pump head 50 ml, ceramic , for aqueous solutions AHC22 Pump head 50 ml, Hastelloy-C, for corrosive chemicals AHC23 AHB32 Pump accessories | 38 Replacement pump heads for preparative AZURA® pumps Pump head 100 ml, stainless steel A4029-1 Pump head 100 ml, titanium A4029V2 Pump head 250 ml, stainless steel A4021-1 Pump head 250 ml, titanium A4021V2 Pump head 500 ml, stainless steel A4038-1 Pump head 500 ml, titanium A4038V2 Pump head 1000 ml, stainless steel A4022-1 Pump head 1000 ml, titanium A4022V2 A4029-1 Check valves Check valve unit for 10 and 50 ml pump heads, for dosing applications A06840 Check valve unit for 10 ml (AZURA® Pump P 6.1L/P 4.1S/ P 2.1S, BlueShadow 40P) A06841 Check valve unit (spring-loaded) for 10 ml and 50 ml pump head A068411 (AZURA® Pump P 6.1L/P 4.1S/ P 2.1S, BlueShadow 40P) Check valve unit (KEL-F) for 10 ml pump head A068412 (AZURA® Pump P 2.1S/P4.1S, BlueShadow 40P) Check valve unit for 50 ml (AZURA® Pump P 6.1L/P 4.1S/ P 2.1S, BlueShadow 40P) A06842 Check valve unit (KEL-F) for 50 ml pump head A068422 (AZURA® Pump P 2.1S/P4.1S, BlueShadow 40P) Check valve unit PEEK/stainless steel for 500 ml and 1000 ml pump heads A1080 (AZURA® Pump P 2.1L, BlueShadow 80P) Check valve unit PEEK/titanium for 500 ml and 1000 ml pump head A1080V1 (AZURA® Pump P 2.1L, BlueShadow 80P) Check valve unit (Kel-F/titanium) for 500 ml and 1000 ml pump head A1080V2 (AZURA® Pump P 2.1L, BlueShadow 80P) Check valve unit Kel-F/stainless steel for 500 ml and 1000 ml pump head (AZURA® A1080V3 Pump P 2.1L, BlueShadow 80P) Check valve unit stainless steel/PEEK for 100 ml and 250 ml pump head (AZURA® A1122 Pump P 2.1L, BlueShadow 80P) Check valve unit titanium/PEEK for 100 ml and 250 ml pump head A1122-1 (AZURA® Pump P 2.1L, BlueShadow 80P) Check valve unit titanium/KEL F for 100 ml and 250 ml pump head A1122-2 (AZURA® Pump P 2.1L, BlueShadow 80P) Check valve unit stainless steel/KEL F for 100 ml and 250 ml pump head A1122-3 (AZURA® Pump P 2.1L, BlueShadow 80P) Maintenance kits Maintenance kit for AZURA® Pump P 2.1S/ P 4.1S, AZURA® Pump P 6.1L, A96423 BlueShadow 40P, 10 ml pump head, including 1 set of gaskets, 2 piston rods, 2 sapphire backing rings, 2 O-rings Maintenance kit for AZURA® Pump P 4.1S & P 2.1S, AZURA® Pump P 6.1L, A96424 BlueShadow 40P, 50 ml pump head, including 1 set of gaskets, 2 piston rods, 2 sapphire backing rings, 2 O-rings Maintenance kit 100 ml for AZURA® Pump P 2.1L and BlueShadow 80P, A96425 including 2 sets of gaskets, 2 piston rods, 2 springs Maintenance kit 250 ml for AZURA® Pump P 2.1L and BlueShadow 80P, A96426 including 2 sets of gaskets, 2 piston rods, 2 springs Maintenance kit 500 ml for AZURA® Pump P 2.1L and BlueShadow 80P, A96427 including 2 sets of gaskets, 2 piston rods, 2 springs Maintenance kit 1000 ml for AZURA® Pump P 2.1L and BlueShadow 80P, A96428 including 2 sets of gaskets, 2 piston rods, 2 springs 39 | Pump accessories Maintenance kits Rebuild-Kit for one pump head AZURA® Pump P 2.1L and BlueShadow 80P A58211 (100ml/250 ml), Venting screw KEL-F, Check valve unit KEL-F, O-ring Rebuild-Kit for one pump head AZURA® Pump P 2.1L and BlueShadow 80P A58212 (500ml/1000 ml), Venting screw KEL-F, Check valve unit KEL-F, O-ring Rebuild-Kit Kel-F for AZURA® Pump P 4.1S/P 2.1S/P6.1L/40P, 10 ml/min pump head A5821-1 Rebuild-Kit Kel-F for AZURA® Pump P 4.1S/P 2.1S/P6.1L/40P, 50 ml/min pump head A5821-2 Maintenance tool kit for 10 ml pump heads A9670 Maintenance tool kit for 50 ml pump heads A9671 Maintenance tool kit for 100 ml pump heads A9672 Maintenance tool kit for 250 ml pump heads A9673 Maintenance tool kit for 500 ml pump heads A9674 Maintenance tool kit 1000 ml pump heads A9675 LPG modules LPG module for Pump P 2.1L binary up to 800 ml/min (stainless steel) AZZ00AA LPG module for Pump P 2.1L ternary up to 220 ml/min (stainless steel) AZZ00AB LPG module for Pump P 2.1L ternary up to 220 ml/min (PEEK) AZZ10AB AZZ00AB Temperature control Pump head cooling and heating device for 100/250/500/1000 ml/min pump heads A2034-1 Pump head cooling and heating device for 10 and 50 ml/min pump heads A2035-1 Temperature controller for column heating sleeve A57024 Heating solution for 10 and 50 ml/min pump heads, includes heating plate and A57036 insulation sleeve Heating solution for 10 and 50 ml/min pump heads, includes heating plate and A57037 insulation sleeve (without temperature controller) A57036 Buffer tubings Buffertubing for AS 6.1L, 500 µl; Spare part for AAA00AA, AAA01AA, AAA10AA, A500525 AAA11AA, AAA50AA & AAA51AA Buffertubing for AS 6.1L, 1000 µl; Spare part for AAA20AA & AAA21AA A500526 Buffertubing for AS 6.1L, 2000 µl; Spare part for AAA31AA, AAA40AA & AAA41AA A500527 Rotor seals A500526 Rotor seal for AS 6.1L, 700 bar; Spare part for AAA00AA & AAA01AA A500519 Rotor seal for AS 6.1L, 860 bar; Spare part for AAA50AA & AAA51AA A500520 Rotor seal for AS 6.1L, 1240 bar; Spare part for AAA10AA & AAA11AA A500521 Rotor seal for AS 6.1L, 345 bar; Spare part for AAA20AA & AAA21AA A500522 Rotor seal for AS 6.1L, 200 bar; Spare part for AAA40AA & AAA41AA A500523 Rotor seal for AS 6.1L, 200 bar; Spare part for AAA31AA A500524 A500521 Autosampler accessories | 40 Syringes 250 µl Syringe for AS 6.1L; Spare part for AAA00AA, AAA01AA, AAA10AA, A500512 AAA11AA, AAA50AA & AAA51AA 500 µl Syringe for AS 6.1L; Spare part for AAA20AA & AAA21AA A500513 2500 µl Syringe for AS 6.1L; Spare part for AAA31AA, AAA40AA & AAA41AA A500514 A500512 Sample loops Sample loop incl. fittings, 100 µl, stainless steel; Spare part for AAA00AA, AAA01AA, A50077 AAA50AA & AAA51AA Sample loop incl. fittings, 10 μl, stainless steel; Spare part for AAA10AA & AAA11AA A50078 Sample loop incl. fittings, 10 ml, stainless steel; Spare part for AAA40AA & A500509 AAA41AA Sample loop incl. fittings, 100 µl, PEEK; Spare part for AAA20AA & AAA21AA A500510 Sample loop incl. fittings, 10 ml, PEEK; Spare part for AAA31AA A500511 A50077 Sample needles Sample needle for preparative autosampler AS 6.1L; Spare part AAA40AA & A500516 AAA41AA Sample needle for biocompatible autosampler AS 6.1L; Spare part AAA20AA & A500517 AAA21AA Sample needle for bio-preparative autosampler AS 6.1L; Spare part AAA31AA A500518 Sample needle for analytical autosampler AS 6.1L; Spare part for AAA00AA, A64700 AAA01AA, AAA10AA, AAA11AA, AAA50AA & AAA51AA Vial kits for analytical & preparative HPLC Vials: Screw neck vials N9 (ø 11.6 mm), 1.5 ml, clear glass, flat bottom, wide A0638-6 opening, with label & scale, Caps: Screw caps blue with septum silicone beige/PTFE white, 100 pcs each Vials: Screw neck vials N9 (ø 11.6 mm), 1.5 ml, amber glass, flat bottom, wide A0638-7 opening, with label & scale, Caps: Screw caps blue with septum silicone beige/PTFE white, 100 pcs each Vials: Screw neck vials N9 (ø 11.6 mm), 1.5 ml, clear glass, flat bottom, wide A0638-8 opening, with label & scale, Caps: Screw caps blue with septum rubber red/TEF colourless, 100 pcs each Vials: Screw neck vials N9 (ø 11.6 mm), 1.5 ml, amber glass, flat bottom, wide A0638-9 opening, with label & scale, Caps: Screw caps blue with septum rubber red/TEF colourless, 100 pcs each Microinserts 0.1 ml for Screw neck vials N9, 1.5 ml, 100 pcs. A18201-3 Vials: Screw neck vials N18 (ø 22.5 mm), 10 ml, clear glass, round bottom Caps: A15854 Screw caps, magnetic, with septum rubber red/TEF colourless 100 pcs each A0638-9 Air needles Air needle for AS 6.1L; 50 mm protrusion length A500529 Air needle for AS 6.1L; 56 mm protrusion length A500530 Air needle for AS 6.1L; 62 mm protrusion length A50058 Air needle for AS 6.1L; 68 mm protrusion length A500531 Air needle for AS 6.1L; 74 mm protrusion length A500532 A500533 Air needle for AS 6.1L; 80 mm protrusion length A500533 Set of air needles for autosampler AS 6.1L, 1 pc. of each length A50059 41 | Autosampler accessories Maintenance kits Preventive maintenance kit for AS 6.1L/3950 (1240 bar/1000 bar) AAA10AA/ A5009-1 AAA11AA/A50060/A500601/A50070/A500701 Preventive maintenance kit for AS 6.1L/3950 (700 bar) AAA00AA/AAA01AA/ A5009-2 A50080/A50081 Preventive maintenance kit for AS 6.1L/3950 (345 bar, Bio) AAA20AA/AAA21AA/ A5009-3 A50052-1/A50053/A50053-1 Preventive maintenance kit for AS 6.1L/3950 (200 bar, Prep) AAA40AA/AAA41AA/ A5009-4 A50054-1/A50056-1 Preventive maintenance kit for AS 6.1L/3950 (200 bar, Prep-Bio) AAA31AA/ A5009-5 A50055-2 Preventive maintenance kit for AS-1 (860 bar) A63500/A63501/A63502 A5009-6 Vial plates Vial plate for 48x 1.5 ml vials for autosampler 3950 and AZURA® AS 6.1L, 1pcs. A50050 Vial plate for 84x1.5ml and 3x10ml vials for autosampler 3950 and AZURA® AS 6.1L, A500501 1pcs. Prep vial plate for 12 x 10ml for autosampler 3950 and AZURA® AS 6.1L, 1pcs. A500502 Vial plate for 108x 1.5ml vials for Autosampler AZURA® AS 6.1L, 1pcs. A500505 Prep vial plate for 30 x 10ml for autosampler 3950 and AZURA® AS 6.1L, 1pcs. A500507 A500505 Optional accessories Fuse (2.5 A) for AS 6.1L, 2 pcs. A500534 Rectangular bottle (250 ml, PE) for wash or transport solution A500535 Waste tube for AS 6.1L, silicone, 1 m A500536 Waste tube for AS 6.1L, PTFE, 1 m A500537 Flow cells 1/16” 3 mm path length, 2 µl, 1/16”, stainless steel, classical KNAUER flow cell A4042 3 mm path length, 2 µl, 1/16”, 30 bar, biocompatible, classical KNAUER flow cell A4045 10 mm path length, 10 µl, 1/16”, 300 bar, stainless steel, for 50D, S2550 and MW-1 A4061V2 10 mm path length, 10 µl, 1/16”, 300 bar, stainless steel, with heat exchanger one A4061XB sided inlet and outlet, classical KNAUER flow cell 3 mm path length, 2µl, 1/16”, 300 bar, PressureProof Flow cell cartridge for AMB18 AZURA® Detector DAD/MWD 10 mm path length, 2µl, 1/16”, 50 bar, LightGuide Flow cell cartridge for AMC19XA AZURA® Detector DAD/MWD 10 mm path length, 10µl, 1/16”, 300 bar, PressureProof Flow cell cartridge for AMC38 AZURA® Detector DAD/MWD 50 mm path length, 6µl, 1/16”, 50 bar, High Sensitivity LightGuide Flow cell cartridge AMD59XA for AZURA® Detector DAD/MWD AMC19XA 10 mm path length, 2.4 µl, 1/16”, 100 bar, biocompatible, fiber optic connectors, A64150 AMD59XA for PDA-1 Autosampler accessories | 42 Flow cells 1/16” 50 mm path length, 10 µl, 1/16”, 100 bar, biocompatible, fiber optics connectors, A64151 for PDA-1 0.5 mm path length, 3 µl, 1/16”, 200 bar, stainless steel, classical KNAUER flow cell A4069 0.5 mm path length, 3 µl, 1/16”, 100 bar, biocompatible, classical KNAUER flow cell A4095 Flow cells 1/8” 2 mm path length, 1/8”, 200 bar, stainless steel, changeable to 0.5/1.25 mm, A4066 classical KNAUER flow cell 2 mm path length, 1/8”, 100 bar, biocompatible, changeable to 0.5/1.25 mm, A4067 classical KNAUER flow cell Flow cells 1/4” 2 mm path length, 1/4” angular connections, 200 bar, stainless steel, A4068 changeable to 0.5/1.25 mm, without fittings, classical KNAUER flow cell 2 mm path length, 1/4” straight connections, 200 bar, stainless steel, A4068-2 changeable to 0.5/1.25 mm, without fittings, classical KNAUER flow cell A4068 Flow cells 1/16” fiber optics 3 mm path length, 2 µl, 1/16”, 300 bar, stainless steel, fiber optic connectors, A4044 classical KNAUER flow cell 3 mm path length, 2 µl, 1/16”, 300 bar, 85 °C, stainless steel, fiber optic connectors, A4044HT classical KNAUER flow cell Fiber optics adapter kit for AZURA® Detector DAD/MWD, with fiber optic cables AMKX8KIT (1x 400 mm and 1x 750 mm) and mounting bracket 3 mm path length, 2 µl 1/16”, 30 bar, biocompatible, fiber optic connectors, A4047 classical KNAUER flow cell A4044 A4044HT 10 mm path length, 10 µl, 1/16”, 300 bar, stainless steel, fiber optic connectors, A4074 A4074 classical KNAUER flow cell A4089 0.5 mm path length, 3 µl, 1/16”, 200 bar, stainless steel, fiber optic connectors, A4089 classical KNAUER flow cell 0.5 mm path length, 3 µl, 1/16”, 100 bar, biocompatible, fiber optic connectors, A4096 classical KNAUER flow cell Flow cells 1/8” fiber optics 2 mm path length, 1/8”, 200 bar, stainless steel, fiber optic connectors, A4078 changeable to 0.5/1.25 mm, classical KNAUER flow cell 2 mm path length, 1/8”, 100 bar, biocompatible, fiber optic connectors, A4079 changeable to 0.5/1.25 mm, classical KNAUER flow cell A4079 Nano flow cell 3 mm path length, 6 nl, 375 µm OD, 50 µm ID, 300 bar, fused silica, A4104 fiber optic connectors A4104 43 | Autosampler accessories Flow cells larger than 1/8” fiber optics 2 mm path length, 1/4” angular connections, 200 bar, stainless steel, A4081 fiber optic connectors, changeable to 0.5/1.25 mm 2 mm path length, 1/4” TRI-Clamp connections, 80 bar, stainless steel, A4153 fiber optic connectors, changeable to 0.5/1.25 mm 2 mm path length, 3/8” TRI-Clamp connections, 80 bar, stainless steel, A4152 fiber optic connectors, changeable to 0.5/1.25 mm 7 mm path length, 3/8” TRI-Clamp connections, 10 bar, biocompatible, A4152-1 A4152 fiber optic connectors A4153 2 mm path length, 1/2” TRI-Clamp connections, 80 bar, stainless steel, A4154 A4154 fiber optic connectors, changeable to 0.5/1.25 mm A4155 10 mm path length, 3/8” TRI-Clamp connections, 10 bar, biocompatible, A4154-1 fiber optic connectors 2 mm path length, 3/4” TRI-Clamp connections, 80 bar, stainless steel, A4155 fiber optic connectors, changeable to 0.5/1.25 mm Fiber optic cables Fiber optic cables (2 pc), 750 mm, 2x SMA 905 600/660, polymicro A0740 Fiber optic cables (2 pc), custom made sizes,, 2x SMA 905 600/660, polymicro A0743 Fiber optic cables (2 pc), 750 mm, 2x SMA 905 600/660, polymicro, up to 85°C A0740HT A0740 Lamps Deuterium lamp for Smartline S2500 and S2600 detectors A4071 Deuterium lamp for Smartline PDA detectors K-2800, S2800 and S2850 A4447V1 HBST deuterium lamp for AZURA® Detector DAD 6.1L AZL01 HBST deuterium lamp for PLATINblue MW-1 and PDA-1 detectors A64210 Deuterium lamp, replacement, for S2520, 10D, 40D, UVD 2.1S, UVD 2.1L, DAD 2.1L, A5193 MWD 2.1L HBST deuterium lamp for Smartline UV and UV/VIS detectors 2550 A5194 and BlueShadow 50D Halogen lamp for AZURA® Detector DAD 6.1L AZL02 Halogen lamp for converting Smartline UV 2500 detector A4073 into Smartline VIS 2500 detector AZL01 Halogen lamp for converting Smartline UV 2600 detector A4073XA into Smartline VIS 2600 detector Halogen lamp for PLATINblue MW-1 detector A64200 Halogen lamp for PLATINblue PDA-1 detector A64201 Halogen lamp for Smartline 2500 detector, VIS version A4072 Halogen lamp for Smartline 2600 detector, VIS Version A4072XA Halogen lamp for Smartline PDA 2800 and 2850 detectors A4448 Halogen lamp for Smartline UV/VIS detector 2550 A5195 Halogen lamp for upgrading Smartline UV detector 2550 A5197 to Smartline UV/VIS detector 2550 LED for Sedex 80LT and Sedex 85LT light scattering detectors A07541 Xenon lamp for RF-10AXL fluorescence detector A0753 Xenon lamp for RF-20A/Axs fluorescence detector A59210 Autosampler accessories | 44 Waste tubing kits Waste tubing kit for AZURA® RID 2.1L, 1/16” A9841 Waste tubing kit for LightGuide flow cells, 1/16” A9842 Waste tubing kit for UV flow cells, 1/16” A9843 Waste tubing kit for UV flow cells, 1/8” A9844 A9844 Flow splitters Adjustable preparative post column flow splitter. Calibrated on customer flow rate, A5815 split ratio and eluent. Adjustable semi-preparative post column flow splitter. Calibrated on customer flow A5816 rate, split ratio and eluent. Passive post column flow splitter for analytical applications. Calibrated with water for A1770 split ratios of 15:1 - 250:1 Flow splitter for CM 2.1S when used with flowrates over 100 ml/min A5813 A5816 Valves, rotors, stators Description Valve Rotor seal Stator 6 Port 2-position valve, 1200 bar AVC28AC A0611.2 A205118 8 Port 2-position valve, 1200 bar AVC38AC A0611.3 A205120 6 Port 2-position valve, 240 bar, bioinert AVD24CE A205101 A205102 6 Port 2-position valve, 500 bar AVD26AE A205145 A205140 8 Port 2-position valve, 500 bar AVD36AE A205144 A205142 6 Port 2-position valve, 300 bar AVE25AE A205147 A205146 Rotor Seals Manual 6 Port 2-position valve, 240 bar, bioinert AVG24CE A205101 A205102 Manual 6 Port 2-position valve, 1200 bar AVI28AC A0611.2 A205118 Manual 8 Port 2-position valve, 1200 bar AVI38AC A0611.3 A205120 Manual 6 Port 2-position valve, 500 bar AVJ26AE A205145 A205140 Manual 8 Port 2-position valve, 500 bar AVJ36AE A205144 A205142 Manual 6 Port 2-position valve, 300 bar AVK25AE A205147 A205146 8 Port multi-injection valve, 240 bar, bioinert AVN94CE A205131 A205132 16 Port multiposition valve, 500 bar AVQ66AE A205151 A205152 6 Port multiposition valve, 1200 bar AVR28AC A0880.2 A205118 8 Port multiposition valve, 1200 bar AVR38AC A0880.4 A205120 6 Port multiposition valve, 500 bar AVS26AE A205139 A205140 8 Port multiposition valve, 240 bar, bioinert AVS34CE A205103 A205104 8 Port multiposition valve, 300 bar AVS35AE A205141 A205142 Valve AVD26AE 8 Port multiposition valve, 500 bar AVS36AE A205141 A205142 16 Port multiposition valve, 50 bar, bioinert AVS62CE A250105 A205106 6 Port multiposition valve, 300 bar AVT25AE A205148 A205146 8 Port multiposition valve, 50 bar, bioinert AVU32CE A205129 A205130 8 Port multiposition valve, 200 bar AVU34AE A205149 A205150 45 | Autosampler accessories Sample loops 1/16” SST incl. fittings Sample Loop VICI,1 µL, stainless steel, 0.1 mm ID A05641 Sample loop,1 µl, stainless steel, 0.1 mm ID A05642 Sample loop, 2 µl, stainless steel, 0.1 mm ID A05643 Sample loop, 5µl, stainless steel, 0.25 mm ID A05644 Sample loop, 10 µl, stainless steel, 0.25 mm ID A05645 Sample loop, 20 µl, stainless steel, 0.25 mm ID A05646 Sample loop, 50 µl, stainless steel, 0.45 mm ID A05647 Sample loop, 100 µl, stainless steel, 0.45 mm ID A05648 Sample loop, 200 µl, stainless steel, 1 mm ID A0565 Sample loop, 500 µl, stainless steel, 1 mm ID A0566 Sample loop, 1000 µl, stainless steel, 1 mm ID A0567 Sample loop, 2000 µl, stainless steel, 1 mm ID A0568 KNAUER VariLoop S, 10 ml, 1/16”, stainless steel, variable injection volume and A1054-2 multiple injections A1054-2 KNAUER VariLoop L, 40 ml, 1/16”, stainless steel, variable injection volume and A1055-1 multiple injections Sample loops 1/8” SST incl. fittings Sample loop, 1 ml, stainless steel, 2.2 mm ID A1043 Sample loop, 2 ml, stainless steel, 1.6 mm ID A1044 Sample loop, 5ml, stainless steel, 1.6 mm ID A0586-2 A1159-2 Sample loop, 10ml, stainless steel, 2.2 mm ID A0843 KNAUER VariLoop S, 10 ml, 1/8”, stainless steel, variable injection volume and A1159-2 multiple injections KNAUER VariLoop L, 40 ml, 1/8”, stainless steel, variable injection volume and A1160-1 multiple injections Sample loops 1/16” PEEK incl. fittings Sample loop, 10 µl, PEEK, 345 bar, 0.25 mm ID A1058 Sample loop, 50 µl, PEEK, 240 bar, 0.75 mm ID A1060 Sample loop, 100 µl, PEEK , 240 bar, 0.75 mm ID A0508 Sample loop, 200 µl, PEEK, 240 bar, 0.75 mm ID A1061 Sample loop, 500 µl, PEEK, 240 bar, 0.75 mm ID A1057 Sample loop 1 ml, PEEK, 240 bar, 0.75 mm ID A0423 Sample loop 2ml, PEEK, 240 bar , 0.75 mm ID A0785 Superloop, 50 ml, variable injection volumes and multiple injections, A1929 movable piston protects against dilution, 1/16“, Glass, 40 bar A0423 Superloop, 150 ml, variable injection volumes and multiple injections, A1928 movable piston protects against dilution, 1/16“, Glass, 20 bar Sample loops 1/8” PEEK incl. fittings Sample Loop 5ml, PEEK, 50 bar, 1/16“ ID A78980 Sample Loop 10ml, PEEK, 50 bar, 1/16“ ID A78985 Autosampler accessories | 46 Injection syringes for 1/16” injektion port Injection syringe 10 µl A0723 Injection syringe 25 µl A0724 Injection syringe 50 µl A0725 Injection syringe 100 µl A0726 Injection syringe 250 µl A0727 Injection syringe 500 µl A0728 Injection syringe 1000 µ A0729 A0723 Injection syringe 2500 µl A0730 Luer-Lock glass syringes for 1/8” injection port Luer-Lock glass syringe, 10 ml A0573 Luer-Lock glass syringe, 20 ml A0653 A0653 Loop filling ports Loop filling port A0555 Injection Port, stainless steel, 1/16“ A0328 Injection Port, PEEK, 1/16“ A03281 Injection Port, stainless steel, 1/8“ A0505 A0555 Injection Port, PEEK, 1/8“ A05051 A0328 Pack of 12 ampules NaCl calibrating solution, 300 mOsmol/kg A01240 Pack of 12 ampules NaCl calibrating solution, 400 mOsmol/kg A01241-1 Pack of 12 ampules NaCl calibrating solution, 850 mOsmol/kg A01250 Pack of 12 ampules NaCl calibrating solution, 2000 mOsmol/kg A01248 Cleaning tissue, lint-free, for thermistor cleaning A02330 Ribbon cartridge for the plain paper printer A3711 (black) A7014 Printer paper for the plain paper printer A3711 (60 m roll) A7013 A01240 100 Pack of plastic sample tubes for Semi-Micro Osmometer K-7400S A02721 500 Pack of plastic sample tubes for Semi-Micro Osmometer K-7400S A0272 100 Pack of plastic sample tubes for the Semi-Micro Osmometer K-7400S A0720 A0720 A02721 A0272 47 | Osmometry accessories and standards Eluent & column heating Eluent heating device to control the temperature of eluent (1-Channel). A70054V3 Cleanroom compatible. Eluent heating device to control the temperature of eluent A70054V4 and a cloumn heating sleeve (2-Channel). Cleanroom compatible. Temperature controller for column heating sleeve A57024 Heating sleeve for HPLC column 150 x 20 mm HM D=25..57*L=193 mm 100 °C, A57026 230V, 200W, Pt100 Heating sleeve for HPLC column 250 x 20 mm HM D=25..57*L=293 mm 100 °C, A57027 230V, 200W, Pt100 Heating sleeve for HPLC column 150 x 30 mm HM D=38..70*L=203 mm 100 °C, A57028 230V, 400W, Pt100 Heating sleeve for HPLC column 250 x 30 mm HM D=38..70*L=303 mm 100 °C, A57029 230V, 500W, Pt100 A57026 Heating sleeve for HPLC column 150 x 50 mm HM D=60..100*L=211 mm 100 °C, A57030 230V, 500W, Pt100 Heating sleeve for HPLC column 250 x 50 mm HM D=60..100*L=311 mm 100 °C, A57031 230V, 800W, Pt100 Heating sleeve for HPLC costum made up to 350 x 50 mm A57032 Purification Pressure control for delta pressure measurement up to 250 ml/min for 1/16’’ and AZG10 1/8’’. Includes interface box. External pressure sensor up to 250 ml/min for 1/16’’ and 1/8’’. AZG10-1 External pressure sensor for up to 1000 ml/min for 1/4’’, 0 - 10 bar, analog output AZG10-2 External pressure sensor for up to 1000 ml/min for 1/4’’, 0 - 10 bar, LAN AZG10-3 AZG10 External pressure sensor for up to 1000 ml/min for 1/4’’, 0 - 10 bar, LAN, AZG10-4 biocompatible Air sensor (1/16”) for AZURA® Bio LC with one air sensor A70092 and wiring for up to 4 air sensors Additional air sensor for AZURA® Bio LC for 1/16” tubing A70092-1 Air sensor (1/8”) for AZURA® Bio LC with one air sensor A70093 and wiring for up to 4 air sensors Additional air sensor for AZURA® Bio LC for 1/8” tubing A70093-1 Air sensor (1/4“) for AZURA® Bio LC with one air sensor and wiring A70083 for up to 4 air sensors AZG10-2 Additional Air Sensor A70083-1 Don’ forget to order! Power Supply: Distribution Box 24 V for 6 devices like air AZS80SA sensor, external pressure sensor, IFU 2.1 LAN AZURA® Organizer Holder for AZURA® Click for attaching falcons, columns A70085 (5 mm - 26 mm OD) and the pH Flow Cell Clamp for AZURA® Organizer 12 mm A70085-1 Clamp for AZURA® Organizer 16 mm A70085-2 Clamp for AZURA® Organizer 25 mm A70085-3 A70092 AZURA® Click rail for AZURA® L devices for attaching IFU 2.1 LAN, air sensor, A70089 pressure Control and the AZURA® Organizer Purification accessories | 48 K-Connect system K-Connect Fingertight Fitting, PEEK, long, Set of 2, incl. ferrule, A9646 UNF 10/32 Thread for 1/16 inch K-Connect and PEEK Capillaries K-Connect Fingertight Fitting, PEEK, long, Set of 10, incl. ferrule, A9646-1 UNF 10/32 Thread for 1/16 inch K-Connect and PEEK Capillaries A9646/-1 A9645/-1 K-Connect Fingertight Fitting, Stainless Steel, long, Set of 2, incl. ferrules, A9645 UNF 10/32 Thread for 1/16 inch K-Connect Capillaries K-Connect Fingertight Fitting, Stainless Steel, long, Set of 10, incl. ferrules, A9645-1 UNF 10/32 Thread for 1/16 inch K-Connect Capillaries K-Connect Standard Fitting, Stainless Steel, Set of 2, incl. ferrule, A9647 UNF 10/32 Thread for 1/16 inch K-Connect Capillaries A9647/-1 K-Connect Standard Fitting, Stainless Steel, Set of 10, incl. ferrule, A9647-1 UNF 10/32 Thread for 1/16 inch K-Connect Capillaries DYNASEAL system DYNASEAL connection system, 1/16”, 4 short bushings, 4 clamping rings and A0108 8 sealing rings A0108 A0181 DYNASEAL connection system, 1/16”, 3 long bushings, 3 clamping rings and A0181 4 sealing rings DYNASEAL connection system, 1/16”, 10 short bushings, 10 biconical sealing rings A1020 DYNASEAL connection system, 1/16”, 5 long bushings, 5 biconical sealing rings A1069 DYNASEAL connection system, 1/8”, M8x1, 4 long bushings, 4 clamping rings A0736 and 8 sealing rings A1020 A1069 DYNASEAL connection system, 1/8”, M8x1, 4 short bushings, 4 clamping rings A0644 and 8 sealing rings Bushings for capillaries - DYNASEAL DYNASEAL connection system, bushings for 1/16” capillaries, stainless steel, A1021 fingertight, short, 10 pcs. DYNASEAL connection system, bushings for 1/16” capillaries, stainless steel, A1064 A1021 A0735 fingertight, long, 5 pcs. DYNASEAL connection system, bushings for 1/8” capillaries, stainless steel, A1067 fingertight, short, 4 pcs. DYNASEAL connection system, bushings for 1/8” capillaries, stainless steel, A0735 fingertight, long, 4 pcs. A1064 A1067 Split-grooved clamping rings 4 Split-grooved clamping rings for capillaries with 1/16” OD A0484 4 Split-grooved clamping rings for capillaries with 1/8” OD A1239 100 Split-grooved clamping rings for capillaries with 1/16” OD A0482 Sealing rings 30 Sealing rings for capillaries with 1/16” OD, PETP A0139 100 Sealing rings for capillaries with 1/16” OD, PETP A0140 10 Sealing rings for capillaries with 1/16” OD, PEEK A1062 10 Sealing rings for capillaries with 1/8” OD, PETP A0232 10 Sealing rings for capillaries with 1/8” OD, PEEK A1063 49 | Consumables Biconical sealing rings 10 Biconical sealing rings with 1/16”, PEEK A1070 10 Biconical sealing rings with 1/16”, PETP A1022 A1070 10 biconical sealing rings 1/8” PETP A0738 Bushings for capillaries, SST 10 Bushings for capillaries with 1/8” OD, M8x1, wrench caliber 10, stainless steel A0830 3 Bushings for capillaries with 1/16” OD, stainless steel, wrench caliber 1/4”, UNF A0115 10-32, long 10 Bushings for capillaries with 1/16” OD, stainless steel, wrench caliber 1/4”, UNF A0116 10-32, long 10 Bushings for capillaries with 1/16” OD, stainless steel, wrench caliber 1/4”, UNF A0112 10-32, short 25 Bushings for capillaries with 1/16” OD, stainless steel, wrench caliber 1/4”, UNF A0113 10-32, short Ferrules for capillaries 30 Ferrules for capillaries with 1/16” OD, stainless steel A0110 100 Ferrules for capillaries with 1/16” OD, stainless steel A0111 10 Ferrules for capillaries with 1/8” OD, stainless steel A0874 10 Ferrules, Hastelloy, for capillaries with 1/16” OD A01101 10 Ferrules, Titanium, for capillaries with 1/16” OD A01102 Bushings for capillaries, PEEK & polymer Bushing for 1/16” capillaries, PEEK, long Hex, with integrated sealing cone, 5 pcs A25021 Bushing for 1/16” capillaries, PEEK, short Hex, with integrated sealing cone, 5 pcs. A25011 Bushings for 1/8” capillaries, PETP, with integrated sealing cone, M8x1, knurled, A0733 short,10 pcs. Bushings for 1/16” capillaries, with integrated sealing cone, knurled, PEEK, A0584 UNF 10-32, 10 pcs. A0584 A0142 Bushings for 1/16” capillaries, PETP, with integrated sealing cone, knurled, A0145 UNF 10-32, short, 10 pcs. Bushings for 1/16” capillaries, PETP, UNF 10-32, long, 10 pcs. A0144 Bushings for 1/16” capillaries, PETP, knurled, UNF 10-32, short, 10 pcs. A0141 Bushings for 1/16” capillaries, PETP, knurled, UNF 10-32, short, 30 pcs. A0142 A0733 A25021 Flat bottom fittings and adapters Bushings flat bottom for 1/8” capillaries, PEEK, Super flangeless, 1/4-28, 10 pcs. A5829 Bushings flat bottom for 1/16” capillaries, PEEK, Super flangeless, 1/4-28, 10 pcs. A58291 Ferrules for 1/16” capillaries and flat bottom bushings, PEEK, with lock ring A58292 (stainless steel), for Super flangeless bushings, 10 pcs. Ferrules for 1/8” capillaries and flat bottom bushings, PEEK, with lock ring A58293 A58294 (stainless steel), for Super flangeless bushings, 10 pcs. Ferrules for 1/8” capillaries and flat bottom bushings, ETFE, with lock ring A58294 (stainless steel), for Super flangeless bushings, 10 pcs. Adapter PEEK 1/8” flat bottom internal on 1/16” external 10/32 thread A1982 Adapter PEEK 1/8” external thread on 1/16” flat bottom internal thread A05841 Consumables | 50 Blind fittings Blind fittings, 1/16”, PETP, knurled, UNF 10-32, short, 10 pcs. A0146 Blind fittings, 1/16”, PETP, knurled, UNF 10-32, short, 30 pcs. A0147 Blind fittings, 1/16”, PEEK, knurled, UNF 10-32, short, 10 pcs. A0582 Blind fittings, 1/8”, PETP, knurled, M8x1, short, 10 pcs. A0734 A0734 A0146 A0147 Adapters Luer Adapter to 10-32, ETFE, female Luer to male 10/32 threads for injection, A1980 simply screw the adapter in the port of your injection valve A1980 Couplings, polymer/PEEK Coupling to connect 2 capillaries with 1/16’’ OD (material: PEEK/PETP, thread: A0148 UNF10-32), including 2 bushings and sealing rings, 0.5 mm bore, suitable for classical HPLC, 1 pc. Coupling to connect 2 capillaries with 1/16’’ OD (material: PEEK/PETP, thread: A0149 UNF10-32), including 2 bushings and sealing rings, 0.5 mm bore, suitable for classical HPLC, 5 pcs. Coupling to connect 2 capillaries with 1/16’’ OD (material: PEEK, thread: A0233 10-32 UNF), including 2 one-piece PEEK fittings, 0.5 mm bore, suitable for classical HPLC Coupling to connect 2 capillaries with 1/16’’ OD (material: PEEK, thread: A0233-1 10-32 UNF), without fittings, 0.5 mm bore, suitable for classical HPLC Coupling to connect 2 capillaries with 1/16’’ and 1/8”OD (material: PEEK, thread: A1407 10-32 UNF, M8x1), including 2 one piece fittings (1x 1/16”, 1x 1/8“), 1 mm bore Coupling to connect 2 capillaries with 1/8”OD (material: PEEK, thread: M8x1), A14071 including 2 one piece fittings 1/8”, 2 mm bore, suitable for preparative HPLC Couplings, SST/Titanium Coupling to connect 2 capillaries with 1/16’’ OD (material: titanium, thread: A0117V1 10-32 UNF), including 2 bushings and ferrules, 0.5 mm bore, suitable for classical HPLC Coupling to connect 2 capillaries with 1/16’’ OD (material: stainless steel, thread: A0117 10-32 UNF), including 2 bushings and ferrules, 0.5 mm bore, suitable for classical HPLC Coupling to connect 2 capillaries with 1/16’’ OD (material: stainless steel, thread: A0118 10-32 UNF), including 2 bushings and ferrules, 0.5 mm bore, suitable for classical HPLC, 5 sets Coupling to connect 2 capillaries with 1/16’’ OD (material: stainless steel, A0119 thread: 10-32 UNF), including 2 bushings and ferrules, 0.5 mm bore, suitable for classical HPLC, 25 sets Coupling to connect 2 capillaries with 1/8” OD (material: stainless steel, A2512 thread: M8x1), including 2 bushings and ferrules, 2 mm bore, suitable for preparative HPLC Coupling to connect a capillary with 1/16” OD to a capillary with 1/8’’ OD A2513 (material: stainless steel, thread: M8x1, 10-32 UNF), 1 mm bore Coupling Dynaseal to connect a capillary with 1/16” OD to a capillary with 1/8’’ OD A0485 (material: stainless steel, thread: M8x1, 10-32 UNF), including Dynaseal bushings and ferrules (1x 1/16”, 1x 1/8“), 1 mm bore Coupling Dynaseal to connect 2 capillaries with 1/8” OD (material: stainless steel, A0480 thread: M8x1), including 2 Dynaseal bushings and ferrules, 2 mm bore, suitable for preparative HPLC 51 | Consumables Swagelok® unions & reducing unions, SST Union to connect 2 capillaries with 1/4” OD, material: stainless steel, Swagelok® A58263 Reducer to connect a capillary with 3/8” OD to a capillary with 1/4’’ OD, A58264 material: stainless steel, Swagelok® Reducer to connect a capillary with 8 mm OD to a capillary with 1/4’’ OD, A58265 material: stainless steel, Swagelok® Reducer to connect a capillary with 1/8’’ OD to a capillary with 1/4’’ OD, A58266 material: stainless steel, Swagelok® A58270 A58263 Reducer to connect a capillary with 1/16’’ OD to a 1/8’’ OD pipe, A58270 A58271 A58264 material: stainless steel, Swagelok® A58265 A58266 Reducer to connect a capillary with 1/8’’ OD to a 1/4’’ pipe union, A58271 material: stainless steel, Swagelok® Reducer to connect a capillary with 4 mm OD to a 1/8’’ pipe union, A58282 material: stainless steel, Swagelok® T-connectors, SST/titanium T-connector to connect 3 capillaries with 1/8’’ OD A2511 (material: stainless steel, thread: M8x1), including 3 bushings and ferrules T-connector to connect 3 capillaries with 1/16’’ OD A0120 (material: stainless steel, thread: 10-32 UNF), including 3 bushings and ferrules A0120 T-connector to connect 3 capillaries with 1/8’’ OD A58260 (material: stainless steel, Swagelok®) T-connector to connect 3 capillaries with 1/4’’ OD A58261 (material: stainless steel, Swagelok®) T-connector to connect 3 capillaries with 1/4’’ OD (material: titanium, Swagelok®) A58262 A58260 A58261 A58262 T-connectors, polymer T-connector to connect 3 capillaries with 1/16’’ OD A150-1 (material: PEEK, thread: 10-32 UNF), without bushings T-connector to connect 3 capillaries with 1/8’’ OD A2511-1 (material: PEEK, thread: M8x1),including 2 one piece PEEK fittings 1/8” T-connector to connect 3 capillaries with 1/16’’ OD A0150 (material: PETP/POM, thread: 10-32 UNF), inclusive 2 bushings and sealing rings X-connectors, SST X-connector to connect 4 capillaries with 1/16’’ OD (material: stainless steel, thread: A0121 10-32 UNF), including 4 bushings and ferrules X-connector to connect 4 capillaries with 1/8’’ OD (material: stainless steel, thread: A1096 M8x1), including 4 bushings and ferrules X-connector to connect 4 tubings with 1/4’’ OD (material: stainless steel, Swagelok®) A58272 for 1000 ml/ min Systems X-connectors, polymer X-connector to connect 4 capillaries with 1/16’’ OD (material: PEEK, thread: A0151 10-32 UNF), including 4 on e-piece fittings Consumables | 52 Pressure release valves Pressure Release Valve Kit for AZURA® pump P 2.1L and Pump 1800 (25 to 50 bar), A5800 1/8”, stainless steel, cross piece titanium Pressure Release Valve Kit for AZURA® pump P 2.1L and Pump 1800 (3,4 to 24 bar), A5801 1/8”, stainless steel, cross piece titanium Pressure Release Valve for AZURA® pump P 2.1L and Pump 1800 (without spring), A5802 1/4”, stainless steel A5802 Back-Pressure Regulator/pressure relief valve kit for 1/16” OD tubing, stainless steel, A5805 provides a constant back-pressure of 3 bar, contains pressure relief valve tee and fittings for 1/16” Spring for pressure release valve, 25 - 50 bar M1070 Spring for pressure release valve, 3.4-24 bar M1080 M1080 Backpressure regulators Back-Pressure Regulator for 1/16“ OD tubing, 10-32 threads, PEEK, Range 1-20 bar A70087 (15-300 psi) Back-Pressure Regulator for 1/16“ OD tubing, 10-32 threads, PEEK, Range A70088 20-103 bar (300-1500 psi) Back-Pressure Regulator for 1/16“ OD tubing, 10-32 threads, stainless steel, A70084 Range 90-300 bar (1300-4200 psi) Back-Pressure Regulator/pressure relief valve for 1/16” OD tubing, stainless steel, A5805-1 provides a constant back-pressure of 52 bar, contains pressure relief valve tee and fittings for 1/16” Spare membranes for Back-Pressure Regulators A70084, A70087, A70088 A70082 Back-Pressure Regulator/pressure relief valve for 1/8” and 1/16” OD tubing, A5804 134 μl volume, PEEK, provides a constant back-pressure of 1.4 bar (20 psi), contains pressure relief valve tee and fittings for 1/8” and 1/16” A70087 Back-Pressure Regulator for 1/16” OD tubing, 134 μl volume, PEEK, provides a A5804-1 constant back-pressure of 0.3 bar (5 psi), contains Y assembly and fittings Capillaries 1/16”, SST Stainless steel, 1/16” OD, 0.1 mm ID, 300 cm length, 1 pcs. A0130 Stainless steel, 1/16” OD, 0.25 mm ID, 300 cm length, 1 pcs. A0131 Stainless steel, 1/16’’ OD, 0.5 mm ID, 300 cm length, 1 pcs. A0132 Stainless steel, 1/16” OD, 0.7 mm ID, 300 cm length, 1 pcs. A0133 Stainless steel, 1/16” OD, 1 mm ID, 300 cm length, 1 pcs. A0134 Stainless steel, 1/16” OD, 0.1 mm ID, 10 cm length, 10 pcs. A0123 Stainless steel, 1/16” OD, 0.1 mm ID, 20 cm length, 10 pcs. A0124 Stainless steel, 1/16” OD, 0.1 mm ID, 30 cm length, 10 pcs. A0125 Stainless steel, 1/16’’ OD, 0.25 mm ID, 10 cm length, 10 pcs. A0126 Stainless steel, 1/16’’ OD, 0.25 mm ID, 20 cm length, 10 pcs. A0127 Stainless steel, 1/16” OD, 0.25 mm ID, 30 cm length, 10 pcs. A0128 Capillaries 1/16”, titanium Titanium, 1/16” OD, 0.7 mm ID, 50 cm length, 1 pcs. A0506 53 | Consumables Capillaries 1/8”, SST Stainless steel, 1/8”OD, 1.6 mm ID, 150 cm length, 1 pcs. A0639 Stainless steel, 1/8” OD, 2.2 mm ID, 150 cm length, 1 pcs. A0640 Capillaries, AZURA® Analytical K-Connect, stainless steel, 1/32” with fitting sleeve for 1/16”connections Material Color code ID [mm] length [mm] Included in Capillary Kit Article no. Stainless steel red 0.10 150 - AZF41 Stainless steel red 0.10 300 1 x in AZF40 AZF42 Stainless steel red 0.10 400 2 x in AZF40, 1 x in AZF80 AZF43 Stainless steel red 0.10 700 2 x in AZF40, 1 x in AZF80 AZF44 Stainless steel red 0.10 900 1 x in AZF40 AZF45 Stainless steel yellow 0.18 150 - AZF51 Stainless steel yellow 0.18 300 1 x in AZF50, 1 x in AZF80 AZF52 Stainless steel yellow 0.18 400 2 x in AZF50, 1 x in AZF80 AZF53 Stainless steel yellow 0.18 700 2 x in AZF50, 1 x in AZF80 AZF54 Stainless steel yellow 0.18 900 1 x in AZF50, 1 x in AZF80 AZF55 Stainless steel black 0.45 150 - AZF61 Stainless steel black 0.45 300 1 x in AZF60 AZF62 Stainless steel black 0.45 400 2 x in AZF60 AZF63 Stainless steel black 0.45 700 2 x in AZF60 AZF64 Stainless steel black 0.45 900 1 x in AZF60 AZF65 AZURA® Analytical K-Connect start-up kits, stainless steel, 1/32” capillaries with fitting sleeves for 1/16”connections Set of precut capillaries 0.1 mm, fittings, connectors and adapters, red AZF40 Set of precut capillaries 0.18 mm ID, fittings, connectors and adapters, yellow AZF50 Set of precut capillaries 0.45 mm, fittings, connectors and adapters, black AZF60 Set of precut capillaries 0.1 and 0.18 mm ID, fittings, connectors and adapters, AZF80 red/yellow AZURA® Capillary start-up kit, SST All kits contain a special amount of capillaries, fittings, ferrules, couplings, connectors and adapters. AZURA® start-up kit 1/16” stainless steel, 0.25 mm ID precut capillaries AZF70 AZURA® Start-Up Kit 1/4’’ HPG, stainless steel, set of capillaries and fittings A9850-2 AZURA® Start-Up Kit 1/4’’ LPG, stainless steel, Set of capillaries and fittings A9850-3 AZURA® start-up kit 1/16”, stainless steel, semi-prep, capillary kit A9849-1 AZURA® start-up kit 1/8” stainless steel, capillary kit A9850 AZURA® start-up kit 1/16” stainless steel, capillary kit A9849 Accessory kit for ScaleUp system, 1/16”, stainless steel A9850-1 Consumables | 54 AZURA® Capillary start-up kits for special HPLC systems Dedicated Start up kits for special HPLC systems include anything you need for system build-up. AZURA® Start-up Kit PEEK, for Analytical HPLC System, up to 5 ml/min or 300 bar A70501 AZURA® Capillary Start-up kit for educational system 1/16”, stainless steel A9849-2 AZURA® GPC Cleanup Start up kit, Tefzel-(ETFE) tubing, OD 1/16”, ID 0.7 mm A50041 Accessory kit for ScaleUp system, 1/16”, stainless steel A9850-1 Tubing start-up kits for FPLC AZURA® FPLC Start-up kit, PEEK, 1/16” for 10 ml/min FPLC systems A70500 AZURA® FPLC Start-up kit, transparent FEP, 1/16” for FPLC systems up to 10 ml/min A70500A and 20 bar AZURA® FPLC Start-up kit, PEEK/Tefzel, 1/16” for 50 ml/min FPLC systems A70600 AZURA® FPLC Start-up kit, FEP/PEEK, 1/8” for 100 ml/min - 500 ml/min A70300 FPLC systems AZURA® FPLC Start-up kit, PEEK, 1/8” for 100 ml/min - 500 ml/min FPLC systems, A70300A up to 100 bar. AZURA® FPLC Start-up kit, PEEK/Tefzel, 1/16” for FPLC systems up to 100 ml/min A70300B AZURA® FPLC Start-up kit, 1/4” for 1000 ml/min FPLC systems A70400 Tubing 1/16”, PEEK, by the meter 0.13 mm ID, variable length, max. pressure 420 bar, red striped A2522 0.18 mm ID, variable length, max. pressure 400 bar, yellow striped A2523 0.25 mm ID, variable length, max. pressure 385 bar, blue striped A2524 0.50 mm ID, variable length, max. pressure 350 bar, orange striped A2525 0.75 mm ID, variable length, max. pressure 240 bar, green striped A2526 1.00 mm ID, variable length, max. pressure 165 bar, grey striped A2527 1.40 mm ID, variable length, max. pressure 50 bar, black striped A2528 Tubing 1/8” OD, PEEK, by the meter 1.59 mm ID, variable length, max. pressure 220 bar, natural A2540 0.75 mm ID, variable length, max. pressure 345 bar, natural A2541 2 mm ID, variable length, max. pressure 165 bar, natural A2542 Tubing 1/16” OD, Tefzel™, by the meter 0.25 - 0.3 mm ID, variable length, max. pressure 185 bar A0182-1 0.7 mm ID, variable length, max. pressure 115 bar A0183-1 1.0 mm ID, variable length, max. pressure 85 bar A04781-1 Tubing 1/8” OD, Tefzel™, by the meter Tubing 1/8” OD, ETFE, by the metre A0478-1 Tubing, various OD, FEP, fixed length 2.1 mm ID, 300 cm length, FEP tubing, 1/8” OD A9869 0.81 mm ID, 300 cm length, FEP tubing, 1/16” OD A9869-1 55 | Consumables Tubing, various OD, PFA, by the meter 3.96 mm ID, variable length, PFA tubing 1/4’’ OD, by the meter A31891 1.6 mm ID, variable length, PFA tubing 1/8” OD, by the meter A31892 Tubing, various OD, PTFE, by the meter 0.9 mm ID, variable length, 1.6 mm OD A04782-1 1.45 mm ID, variable length, max. pressure < 10 bar, 2 mm OD A0153-1 1.5 mm ID, variable length, max. pressure 35 bar, 3.2 mm OD A0732-1 7 mm ID, variable length, 9 mm OD A1099-1 4.4 mm ID, variable length, 1/4” OD, black, antistatic A3307 1.6 mm ID, variable length, 1/8” OD, black, antistatic A3306 2 mm ID, variable length, 1/8’’ OD A0873-1 0.45 mm ID, variable length, max. pressure 150 bar, 1.6 mm OD A0152-1 3 mm ID, variable length, max. pressure 20 bar, 4 mm OD A0154-1 Inline filters, SST, for HPLC Inline Filter (prep.) 5-10 µm, stainless steel, max. flow rate 1000 ml/min A3381 (for 1/8” tubing) Replacement frit for A3381 5-10 µm, stainless steel, max. flow rate 1000 ml/min A33811 Inline Filter, PEEK body, Stainless steel frit, 1/16”, to protect your column, A00161 with 2 µm pore size, 3 pcs., easily connected directly to any column A3381 UHPLC/HPLC precolumn filter, universal, 0.5 µm titanium frit, set of 5, stainless steel B2 body, up to 1034 bar Frit 0.5 µm, 0.2 µl for Inline Filter, stainless steel with 0.25 mm bore up to 1375 bar, A00164-1 5 pcs. Inline Filter, stainless steel, frit 0.5 µm, 0.2 µl, for 1/16” capillaries, 0.25 mm bore, A00164 up to 1375 bar B2 Inline filters, biocompatible, for FPLC Inline Filter, PEEK/Titanium, 1/16”, biocompatible, to protect your column, A3378 with 2 µm pore size titanium frit Inline Filter, PEEK/Titanium, 1/16”, biocompatible, to protect your column, A3379 with 10 µm pore size titanium frit A3378 Replacement Frits 2 µm for Inline Filter, PEEK/Titanium, biocompatible A3378-1 A3379 Replacement Frits 10 µm for Inline Filter, PEEK/Titanium, biocompatible A3379-1 Inline Filter, PEEK body, Titanium frit, 1/16”, to protect your column, A00162 with 0.5 µm pore size, 3 pcs.,easily connected directly to any column Inline Filter, PEEK body, Titanium frit, 1/16”, to protect your column, A00163 with 2 µm pore size, 3 pcs., easily connected directly to any column Shut-off valves Shut-off valve, PEEK, 1/16”, including connectors (1/4-28 flat bottom) A5811 Shut-off valve, PEEK, 1/8”, including connectors (1/4-28 flat bottom) A5812 A5811 Consumables | 56 Safety cap sets for analytical systems All sets include 1 l eluent bottles and Vici eluent caps with safety air inlet filter, 1 waste can and cap with safety exhaust filter and adapter to connect the AS 6.1L waste tubing, 250 ml bottle for piston backflushing Safety caps sets for AZURA analytical systems for isocratic systems, incl. filters, bottles and fittings A59257 for LPG systems, incl. filters, bottles and fittings A59257-1 for HPG systems, incl. filters, bottles and fittings A59257-2 Eluent waste kit for all AZURA® Analytical systems , incl. filter, waste can and cap A59258 A59257 A59258 Safety caps VICI Cap, GL45 Thread, 3 ports, 1/4”-28 connection, including O-ring EPDM, A59231 nuts and ferrules VICI Safety Cap with stopcocks, GL45 Thread, 3 ports, 1/4”-28 connection, A59234 including O-ring EPDM, nuts and ferrules VICI Safety Cap with stopcocks, GL45 Thread, 4 ports, 1/4”-28 connection, A59235 A59232 A59233 including O-ring EPDM, nuts and ferrules VICI Waste Cap, GL45 Thread, 3 ports 1/4”-28 connection, 1 x 10M x 1 for barbed A59236 hose adapter, including O-ring EPDM, nuts and ferrules VICI Cap, GL45 Thread, 2 ports, 1/4”-28 connection, including O-ring EPDM, A59232 nuts and ferrules VICI Cap, GL45 Thread, 2 ports, 1/4”-28 connection, including O-ring EPDM, A59230 nuts and ferrules VICI Safety Cap with stopcocks, GL45 Thread, 2 ports, 1/4”-28 connection, A59233 A59231 A59234 including O-ring EPDM, nuts and ferrules Safety caps accessories VICI 1/4-28 flangeless nuts, PPS, for 1/16” tubing, for VICI caps, 10 pcs. A59245 VICI plugs, PEEK, 1/4”-28,1 pcs., for closing unused ports for VICI caps A59249 VICI Barbed hose adapter for 1/8” tubing, for VICI caps A59251 VICI Barbed hose adapter for 8 mm ID tubing, for VICI caps A59254 VICI Safety Air Inlet Valve with 15 mm filter, fit any VICI cap or VICI safety cap A59241 A59249 VICI Safety Exhaust Filter with detector, filled with absorbent, fit any VICI cap or VICI A59243 safety cap VICI Safety Air Inlet Valve with 4 mm filter, fit any VICI cap or VICI safety cap A59240 VICI Safety Exhaust Filter filled with absorbent, fit any VICI cap or VICI safety cap A59242 O-ring FEP coated for sealing all VICI caps or VICI safety caps, improved chemical A59244 resistance VICI Safety Exhaust Filter for basic solutions in IC, fit any VICI cap or VICI safety cap A59255 VICI 1/4-28 flangeless nuts, PPS, for 1/8” tubing, for VICI caps, 10 pcs. A59246 VICI inverted ferrules, ETFE, for 1/16” tubing, suitable for A59245, for VICI caps, A59247 10 pcs. A59242 VICI inverted ferrules, ETFE, for 1/8” tubing, suitable for A59246, for VICI caps, A59248 10 pcs. Cellulose filter, 0.2 µm, 4 mm diameter for VICI Safety Air Inlet Valve, fit any VICI cap A59252 or VICI safety cap Cellulose filter, 0.2 µm, 15 mm diameter for VICI Safety Air Inlet Valve, fit any VICI A59253 cap or VICI safety cap AZURA® Tubing kit with cap and solvent filter (A3375, stainless steel, 10 µm), A9650 suitable for all analytical HPLC systems A9650 57 | Lab equipment Consumables Wrenches & tightening tools Torque wrench basic tool, 1-25 Nm, without plug-in head X0219 Open-jaw plug-in head for Torque wrench X0219, 1-17 mm (for 100 - 1000 ml pump X0220 head in-/outlet and LPGblock) X0219 Open-jaw plug-in head for Torque wrench X0219, 1-10 mm (for Smartline I pump X0221 heads) Open-jaw plug-in head for Torque wrench X0219, 1-13 mm (for 10 - 50 ml Smartline X0222 II/AZURA® pump heads in-/outlet) Double open-end wrench, 1/4” and 5/16” X0003 Double open-end wrench, 8/10 mm X0030 Double open-end wrenches, 2 pc., 1/4”and 5/16” A0138 X0030 Tightening tools for PEEK fittings, blue, 1/16“ fittings 1/4“ hex head nut A25030 (10-32 threads) Tightening tools for PEEK fittings, green, 1/32“ fittings 3/16 hex head nut A25031 (6-40 threads) Capillary and tube cutter Tube cutter, suitable for all tubes A0569 Capillary cutter for PEEK capillaries and tubings with OD up to 4 mm A0851 A0851 Metal capillary cutting pliers for 1/16” capillaries A0809 Metal capillary cutter for 1/8” capillaries A9865 A0809 Capillary graters and benders Capillary grater for degrating of 1/16” stainless steel capillaries, can also be used to A0137 remove column filters Capillary grater for degrating of 1/8” stainless steel capillaries A9864 Tube bender for 1/8” and 3/16” tubings with an bend radius of 90° A9870 A9870 Tool sets for AZURA® systems Tool Kit AZURA® for systems with PEEK or pre-cut capillary kits A1033 Tool Kit AZURA® for 1/16” systems (stainless steel) A1033-1 Tool Kit AZURA® for 1/8” systems (stainless steel) A1033-2 Tool sets for AZURA® pump heads A1033 A1033-1 A1033-2 Tool Kit for 10 ml pump head A9670 Tool Kit for 50 ml pump head A9671 Tool Kit for 100 ml pump head A9672 Tool Kit for 250 ml pump head A9673 Tool Kit for 500 ml pump head A9674 Tool Kit for 1000 ml pump head A9675 Lab equipment | 58 LC racks Benchtop rack: AZURA® S 300 x 160 x 210 mm (WHD) A70016 Benchtop rack: AZURA® L low 480 x 190 x 420 mm (WHD) A70010 Benchtop rack: AZURA® L high 480 x 430 x 420 mm (WHD) A70011 Benchtop rack: special manufacture with customized dimensions A70015 A70011 Product Riser AZURA®: Set of 4 feet that lift the device to a height of 28 mm for easy A9860 handling of the waste tube of the drainage system AZURA® mounting brackets A9860 Mounting bracket AZURA® L for KNAUER manual injection valves A9853 Mounting bracket AZURA® L for Vici valve drives A9853-2 Mounting bracket AZURA® L for columns with 25 - 29 mm AD A9853-3 Mounting bracket AZURA® L for KNAUER flow cells A9853-5 Mounting bracket AZURA® L for prep sample loop A9853-6 Mounting bracket AZURA® L for Hypershear mixing chambers A9853-8 Mounting bracket AZURA® L Bio for manual KNAUER injection valve, pH-flowcell A9854-1 and 2 prepacked columns Mounting bracket AZURA® S for manual KNAUER injection valve A9854-2 A9853 Mounting bracket AZURA® L for AZURA® Valve Unifier VU 4.1(both-sided) or A9854-3 AZURA® UVD 2.1S and AZURA® CM 2.1S (left-sided on AZURA® L) LC column holder/multi column base A9847 A3983 Column holder: Magnetic clip, for all columns with 1/4” OD, for all KNAUER columns A9847 with 3, 4 and 4.6 mm ID, compatible with all AZURA® devices Prism column holder for horizontal storage of HPLC columns on the lab bench, the A3983 most price attractive alternative to store your HPLC columns Glass column holder, Stand, plate and 2 clamps, can hold one glass column in the A1319 dimensions of 10-40 mm ID Multi Column Base Bio 60 x 40 x 130cm (w x d x h) for up to 3 MPLC columns with A70190 A1319 A0070A conn. for cooling device Multi Column Base serves as a holder for up to 3 columns with inner diameter up to A0070A 50 mm, especially made for preparative column solutions A4364 Accessories for LC column holder 3-finger clamps, long shaft, finger with silicone coating, clamp width A4364 12-100 one piece 3-finger clamps, short shaft, finger with silicone coating, clamp width A4364-1 12-100, one piece A4368 Clamp for Multi Column Base, short shaft, to fix an HPLC column or other A4368 accessories to the Multi Column Base Clamp for Multi Column Base, long shaft, to fix an HPLC column or other accessories A2820 to the Multi Column Base Bosshead squared for Multi Column Base, used in combination with clamps with a A2820A A2820 long shaft Installation accessories HPLC Standard accessory kit A1071 Installation Box Kit, Box for small parts, KNAUER file folder and support sticker A9862 A9862 59 | Lab equipment A PV procedure is recommended for testing newly installed AZURA® HPLC systems as well as for regular monitoring of system performance. This table gives an overview of the needed PV document, PV Standard and HPLC column for a specific AZURA system. Backpressure Type of Flow cell Injection: PV document Article no. of PV Article no. of range detection path length Symple loop standard HPLC column [mm] volume [μl] UHPLC systems UV, DAD 10 1 – 20 VPV-001: Analytical HPLC, A01260-3 10BE181E2F (max. 1000 bar) UV detection UV, DAD 10 21 – 100 VPV-001: Analytical HPLC, A01260-2 10BE181E2F UV detection UV, DAD 50 1 – 20 VPV-001: Analytical HPLC, A01260-2 10BE181E2F UV detection UV, DAD 50 21 – 100 VPV-001: Analytical HPLC, A01260-1 10BE181E2F UV detection FLD all 1 – 20 VPV-004: Analytical HPLC, A01262-2 10BE181E2F FL detection FLD all 21 – 100 VPV-004: Analytical HPLC, A01262-3 10BE181E2F FL detection HPLC Plus systems UV, DAD 10 1 – 20 VPV-001: Analytical HPLC, A01260-4 15WE181E2J (max. 700 bar) UV detection UV, DAD 10 21 – 100 VPV-001: Analytical HPLC, A01260-3 15WE181E2J UV detection UV, DAD 50 1 – 20 VPV-001: Analytical HPLC, A01260-3 15WE181E2J UV detection UV, DAD 50 21 – 100 VPV-001: Analytical HPLC, A01260-2 15WE181E2J UV detection FLD all 1 – 20 VPV-004: Analytical HPLC, A01262-1 15WE181E2J FL detection FLD all 21 – 100 VPV-004: Analytical HPLC, A01262-2 15WE181E2J FL detection HPLC & UHPLC RID all all VPV-002: Analytical HPLC, RI A01261-1 05WE184E2J systems detection Preparative HPLC UV, DAD < 2 all VPV-007: Preparative HPLC, A01264-1 05JE181E2J systems UV detection UV, DAD > 2 all VPV-007: Preparative HPLC, A01264-2 05JE181E2J UV detection RID all all VPV-008: Preparative HPLC, A01265-1 05IE184E2J RI detection Standards for PV | 60 PC Hardware & Software With the hand-held Mobile Control and Mobile Control Chrom software you have your AZURA devices and systems at your fingertips. Remotely control and monitor your devices and enjoy the touchscreen-optimized user interface. Choose Mobile Control as an easy-to-use and cost-effective device control solution! Mobile Control provides full access to AZURA devices. Change device settings, set operating parameters, automate device control or check the system status… Mobile Control features all functionalities of a device display. Do you want to acquire data without the overhead of an advanced chromatographic data system? Mobile Control Chrom features data acquisition from AZURA detectors in addition to full device control. Why to use Mobile Control (Chrom) software Only pay for what you use: Mobile Control features basic functions to operate AZURA devices and systems. Mobile Control can operate dedicated applications which do not require a highly developed and cost-intensive Chromatographic Data System (CDS). Save space: Mobile Control runs on a tablet. Especially in labs with little space avoiding a desktop PC with keyboard and monitor can be a decisive factor. The touch-optimized user interface allows device control using just your fingers. Save time: Mobile Control convinces due to an intuitive user interface and a clearly structured menu function. The training period is minimal in comparison to a complex CDS. Free updates: With every release new features are available in Mobile Control. You can download the current version for free. Free trial: To evaluate if Mobile Control holds up to your expectations, you can download the software and test the free trail option. Perfect for those who’d like to try before they buy. Customized software design: Mobile Control is made by KNAUER and can be adapted to the requirements of our OEM partners. Specifications Software Software name Mobile Control - display software for AZURA devices without data This software supports a acquisition wide range of instruments: Mobile Control Chrom - display software for AZURA devices with data www.knauer.net/softwarecontrol acquisition Operating system Windows 10 Field of application Display software, device control, basic LC system operation For PC hardware & periphery see p. 67 Supported instruments most AZURA® devices Expandability Stand-alone yes Multi-user environment yes Free demo version: Report functions yes www.knauer.net/mobilecontrol Ordering details: Software A9607 Mobile Control without data acquisition including tablet and mount A9608 Mobile Control Chrom with data acquisition including tablet and mount A9610 Mobile Control without data acquisition A9612 Mobile Control Chrom with data acquisition A9613 Mobile Control Chrom with data acquisition and column test option A9614 Upgrading Mobile Control to Mobile Control Chrom gaining data acquisition Accessories A96181 USB-LAN ADAPTER Network adapter USB 2.0 <-> 10/100 Ethernet for tablets A64809 WiFi router, 8x LAN GBit RJ-45 ports, 1x WAN GBit RJ-45 port A64809INT WiFi router, 8x LAN GBit RJ-45 ports, 1x WAN GBit RJ-45 port, power pl ug UK, US or AUS A64811 Single device WLAN router for Mobile Control - 1x RJ45, 10/100 MBit; WLAN A9615 Tablet lock with stand for all tablets A9616 Tablet lock for all tablets A9617 Mobile Control Mount - flexible tablet mount for tablets Mobile Control (Chrom) 61 | Software for Windows 10 KNAUER ClarityChrom® is a powerful, yet easy-to-use chromatography software (or chromatography data system, CDS) for instrument control, data acquisition and data processing. ClarityChrom is designed for smaller laboratories. It is an economical solution compared to other more complex chromatography software while still offering FDA 21 CFR Part 11 compliance. ClarityChrom comes as a complete package with LC control and including autosampler control. It is scalable from 1 up to 4 systems; depending on the desired instruments. The built-in fractionation option as well as the optional extensions as SST for automated system tests, PDA for 3D (UV spectra) data handling, GPC analysis, MS and GC control cover a wide range of the requirements for a CDS on a modern lab. KNAUER additionally offers are more advanced fractionation with the KNAUER FRC control module. ClarityChrom supports all KNAUER devices that can be controlled by software. Please refer to the instrument support list in the Support section of our website, the download link can be found below. Beside this, devices and systems from more than 45 manufacturers can be controlled. Additionally, data acquisition can also be performed with any detector providing a voltage output by simply connecting a KNAUER IFU 2.1 interface box or any other supported A/D converter. The system suitability (SST) extension automates the calculation of system suitability parameters for system validation and calculates up to 12 parameters and compares the results with the limits the user has set. The PDA extension allows to acquire and process 3D data from a photo diode array detector (KNAUER PDA detectors are fully supported). The PDA extension provides peak purity analysis and peak identification by spectral library search in self-made or commercial spectra libraries. The SEC/GPC extension provides interactive and automated gel permeation chromatography analysis, including re-calibration and GPC reporting, as well as simplifies the retrieval of GPC data. The GPC extension allows flow rate and multi-detector delay corrections and includes Narrow, Broad and Broad on Narrow calibrations. ClarityChrom comes with some basic fractionation functionality. The KNAUER-exclusive Knauer FRC control module for ClarityChrom adds more drivers of several fraction collectors and supports the peak recognition by level and/or slope as well as fractionation by time. Also more advanced functionality as solvent recycling, manual fractionation and rack view with detailed fraction information and chromatogram links are available. The functionality corresponds exactly to the KNAUER preparative functionality of discontinued ClarityChromPrep. ClarityChrom offers all the necessary operations for an analytical lab. Moreover, the preparative version adds fractionation options to this feature list and allows more flexibility in the lab. Clari-This software supports a tyChrom is the best solution for all laboratories searching for an up-to-date and robust software wide range of instruments: with support of devices from many manufacturers to be flexible in instrumentation but also meet www.knauer.net/en/supp_cc the requirements for modern laboratories. For PC hardware & periphery Specifications see p. 67 Software Software name ClarityChrom Extensions / Licenses PDA / 3D UV, System suitability, Fraction collection, SEC/GPC, Mass spectrometry System architecture 32-bit CDS Operating system Windows 10, Windows 8.1, Windows 7, all 32- and 64-bit Expandability Stand-alone Workstation version, max. 4 systems controlled by one computer, max. 3 LC systems, max. 2 systems with PDA or 1 system with MS or special devices per computer Client/server No Client/Server functionality Multi-user environment Selectable system of user accounts with independently customizable behavior and appearance for individual users Network environment Easy offline data sharing (at the file level) among all stations in a local network Fields of application Analytical and preparative HPLC, GPC/SEC, GC, MS Supported instruments All Knauer devices are supported, driver for devices from many other manufacturers are available Instrument connection Supports RS-232, Ethernet, PCI interface card, A/D-D/A interface Recommended PC hardware Pentium 2 GHz, 4 GB RAM, 80 GB free hard disk space, separate graphics card if one PC should control more than one system, USB for dongle, connectors as LAN, RS-232 etc. for device control Graphics capabilities Multiple chromatogram view and overlay, PDA view ClarityChrom® Software | 62 Expandability Integration 27 integration parameters (peak width, threshold, tangent slope ratio etc.) integration parameters programmable in time, automatic re-integration Calculation types with/without calibration (int./ext. standard method) Security and GLP Installation qualification test of the software; FDA 21 CFR Part 11 conformance, validation with virtual detector Instrument control method-based instrument control, Instrument status display and Direct-Control mode, Calibration 6 types of calibration curves, up to 20 levels, reference peaks, groups, unlimited number of standards (peaks), LOD, LOQ Chromatogram operations Overlay view, custom labels and settings, also applying mathematical operations to chromatograms Automation Sequences, automatic launch of selected commands or applications immediately following chromatogram acquisition – Post run, Batch Presentation of results Integrated customizable table of results, columns with userdefined calculation, summary table, and export in text or database format Calculations Custom: 12 predefined mathematical operators, 15 basic and 4 summary functions, special: Kovats indexes for GC, determination of noise/drift, performance calculations Data import and export ASCII, AIA, dBase Additional options/extensions MS option separate license option; control and data acquisition from supported QUAD-MS FRC option separate license option; Control of fraction collectors and KANUER valve drives as fraction collector, fractionation per time/Level/Slope, rack info with filling level and chromatogram link PDA option separate license option; 3D chromatogram, peak purity analysis, spectrum search in self-made or commercial spectra library GPC/SEC option separate license option; molecular weight determination in size exclusion chromatography with various calibration methods System suitability test separate license option; automates the calculation of system suitability parameters for system validation Note Autosampler control included beside Educational license Ordering details: A1670 ClarityChrom® single instrument license for one time base A1674 ClarityChrom® offline license for data evaluation A1671 ClarityChrom® additional instrument license on additional time base A1676 ClarityChrom® option for PDA data processing A1677 ClarityChrom® system suitability option A1678 ClarityChrom® option for GPC data processing A1679 ClarityChrom® option for MS data processing A1682 ClarityChrom® KNAUER FRC control module for preparative HPLC A1681 Upgrade for one system from former version to ClarityChrom® A1687 Upgrade for former ClarityChrom® Prep to latest ClarityChrom® with KNAUER FRC control module A1690 30-day trial version of ClarityChrom A1675 ClarityChrom® university package one offline license 63 | Software PurityChrom is a volume-based chromatography software especially designed for the area of bio purification and FPLC applications. PurityChrom provides a very user friendly and clearly structured interface. The system visualisation offers a graphical representation and easy handling of the complex flow processes. Furthermore each device which is displayed in the fluidic scheme can be manually controlled giving the opportunity to optimize and change and adapt your conditions spontaneously during the run. PurityChrom includes intuitive data evaluation with peak recognition and integration. Due to a very high flexibility methods can be developed according to specific demands. You have the option to create a method based on volume, column volume or time. There is also a possibility to pause the method during the run due to the hold function providing you with complete control over your chromatography process. Solvent visualisation calculates the consumption of solvent for the current run and prevents your column from running dry whereas waste management prevents the loss of your sample. For fractionation you can use a fractionation valve as well as a fraction collector. Current guidelines and regulations like 21 CFR part 11 are entirely supported. PurityChrom allows flexible support of different devices from KNAUER and other manufactures. In our new Version of PurityChrom you can define important functions in your method with Variable values. This allows you to write methods that can be adapted more flexibly to a specific sample or column, just before the run with only one click. In combination with the also newly implemented Sequence Table a quick and easy method scouting provides you with the best method for your purification problem in less time. Furthermore we realized the fractionation using a minor pump and the display of lamp lifetime, to give you even more control over you purification System. Specifications Software Software name PurityChrom® 5 Operating system Windows XP, Windows Vista, Windows 7, Windows 8, Windows 10 Expandability Stand-alone Extension to unlimited number of devices and 8 data channels, autosampler supported This software supports a Fields of application FPLC wide range of instruments: Recommended PC hard- CPU/Memory: Pentium III or higher with at least 1 MHz32 or 64 bit www.knauer.net/softwarecontrol ware At least 512 MB RAM (Windows XP) and 2 GB (Windows Vista and higher) Graphics: Screen with minimal resolution 1024 x 768 For PC hardware & periphery Connectors and Slots: USB for license dongle/COM, USB or LAN see p. 67 according to connected instruments Integration Real-time analysis of peaks, automatic or manual integration and baseline correction Security and GLP FDA 21 CFR Part 11 Chromatogram operations overlay view Automation Sequences Presentation of results Individual report configuration Calculations Column performance calculations according to DAB Data import and export Comma Separated Value, AIA/ANDI, ChromStar Slice Special features Method creation based on volume or column volume, Hold function, Functionalised visualisation, Direct Control during a run, Display of solvent supply, Waste management Additional options/extensions FRC option Included Ordering details: Device A2650 Basic License for one system A2652 Extends the Basic License to an unlimited number of controllable devices and 8 data channels, adds autosampler and stacked injections support A2654 3D option for a diode-array detector (DAD) A2656 PurityChrom® Maintenance and Support including free updates and 5 Hours Software support by KNAUER PurityChrom® Software | 64 OpenLAB CDS EZChrom Edition is the next generation of chromatography data systems and the successor of ChromGate CDS. OpenLAB CDS EZChrom Edition provides chromatography data acquisition, processing and control of GC and LC chromatographs and is used in chromatography operations ranging from single user/single instrument to multi-user/multi-instrument laboratories. It provides support of devices from KNAUER and many other manufacturers. The basic workstation license can only be installed on one PC and allows for control and data acquisition from one system. The license includes System Suitability, Fraction Collector Control and one year Software Maintenance Agreement (SMA). The system suitability option allows for test if the system is suitable for particular analysis by testing several parameters as resolution, peak asymmetry and theoretical plates. The KNAUER fraction collector control option includes the drivers of several fraction collectors and supports fractionation by time, the peak recognition by level and/or slope, also with spectral confirmation. Collet Slices allows for setting a desired volume for each fraction, within the defined fraction vial volume. In the fraction collector configuration the delay volume and the fraction vial volume can be defined. This ensures that the target substance will be collected in the fraction vial and the fraction vial will not overflow. The pump flow rate, which is required for calculation of delay and fraction vial fill level, will be automatically read from the pump’s meethod setup. If a chromatogram of your separation already exists, the required fractionation commands can be derived directly from the chromatogram with a double mouse-click. The rack view gives an overview of the already collected fraction, their volume and retention time. The manual fraction control and This software supports a the option to use the KNAUER electric valves for fractionation gives you more flexibility. The com-wide range of instruments: bination of virtual detector and virtual fraction collector allows for optimizing the fractionation www.knauer.net/softwarecontrol settings from an existing chromatogram of your separations without any physically existing device and, therefore, without the loss of solvent or target substance. For PC hardware & periphery OpenLAB EZChrom Edition and EZChrom Elite are registered trademarks of Agilent Technolo-see p. 67 gies, Inc. Specifications Software Software name OpenLAB CDS EZChrom Edition Extensions / Licenses Fraction collection, System suitability, PDA / 3D UV System architecture 32-bit CDS Operating system Depends on CDS version. Latest version, supported by KNAUER drivers, is A.04.08. It runs on Windows Prof/Enterprise 10 64-bit and Windows 7 Prof. 32- and 64-bit. Additional options/extensions FRC option always included, for preparative HPLC, adds tools for detector controlled fraction collection, solvent and peak recycling, stacked injection, rack view with information about RT and volume FRC features fractionation can be controlled by time (volume), level, slope including AND/OR combination of these criteria, spectra comparison, local maximum and local minimum, slices, full manual control of fractionation during a run PDA option 3D chromatogram, peak purity analysis, spectrum search in self-made or commercial spectra library (must be converted in OpenLAB spectral library format), separate license option GPC/SEC option license is discontinued System suitability test license always included, automates the calculation of system suitability parameters for system validation Ordering details: A2600-1 OpenLAB® CDS EZChrom Edition workstation for one system with SMA and 4x System Suitability A2610-1 OpenLAB® CDS EZChrom Edition 3D option for UV detectors MW-1, 2550 and 2600 A2611-1 OpenLAB® CDS EZChrom Edition 3D UV Option for DAD DAD6.1L, DAD2.1L, PDA-1, S2850 A2618-01 OpenLAB® CDS EZChrom Edition drivers for 80LT, 85LT, 90LT, 100LT and LC from Sedere A2602-1 OpenLAB® CDS EZChrom Edition Instrument Control License A2614-1 OpenLAB® CDS EZChrom Edition for distributed systems - please ask for desired configuration OpenLAB® CDS EZChrom Edition 65 | Software Thermo Scientific™ Dionex™ Chromeleon™ is one of the most wide-spread chromatography data systems. Its intuitive handling benefits laboratory workflow and the highly developed algo-rithms simplify data processing. It offers a broad range of third-party drivers and can be easily used with existing HPLC systems. KNAUER offers drivers for a lot of its devices. Disclaimer: KNAUER Wissenschaftliche Geräte GmbH is solely responsible for development, testing and support of Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data System driver software for KNAUER instruments and therefore solely liable for damages associated with the use of this driver software. Specifications Computer requirements Operating system Windows 8.1 Professional, 64-bit;Windows 7 SP1 Professional, This software supports a Enterprise, 64-bit, (32-bit version is not recommended); Windows wide range of instruments: Vista SP2 Business, Ultimate, 32-bit (Vista is not recommended) www.knauer.net/softwarecontrol CPU (recommended) 3 GHz Intel Core i7 or better Memory RAM (reccomended) 8 GB For PC hardware & periphery Free Hard Disk Space 120 GB available, for system with PDA detectors see p. 67 Optical Drive DVD Display (recommended) 1280 x 1024, 32-bit color USB Ports 1 port for USB license key Ethernet Port 1 port for router (for system connection) Ordering details: Drivers A1783-4 Sedex Driver for Chromeleon 7.2; For Sedex 85LT / 90LT; Instrument Controler Class 3 necessary A1783-5 Sedex Driver for Chromeleon 7.2; For Sedex FP / LC / 100LT; Instrument Controler Class 3 necessary A1783-3 Shimadzu LC Driver for Chromeleon 7.2 Shimadzu CBM-20A required A1783-2 Thermo Scientific™ Dionex™ Chromeleon™ Driver CD (AZURA® only) Enterprise A1791-1 Thermo Scientific™ Dionex™ Chromeleon™ 7.2 Enterprise. Complete Software Package incl. Secure Client Lizenz: Data Client, Instrument Operation, Report Designer Pro, Compliance Tools A1792-1 Thermo Scientific™ Dionex™ Chromeleon™ 7.2 Enterprise license - enables full control of one 3rd Party LC Instrument, includes Instrument Controller, Spectral, Fraction Collection and one Class 3 license Workgroup A1780-2 Thermo Scientific™ Dionex™ Chromeleon™ 7.2 Bundle Workstation Open. Complete Software Package incl. License Dongle A1782-2 Thermo Scientific™ Dionex™ Chromeleon™ 7.2 Instrument Controller Option - Instrument Class 3. Max 2 LC per workstation A1787-2 Thermo Scientific™ Dionex™ Chromeleon™ 7.2 Instrument Controller Option - MS License A1784-2 Thermo Scientific™ Dionex™ Chromeleon™ 7.2 Instrument Controller Option - PDA License Chromeleon™ 7.2 Drivers Software | 66 Desktop PCs Desktop PC (SFF) for OpenLAB® and Chromeleon™ with 24” monitor, English A13121 edition Windows 10 Prof. 64-bit Englisch, Intel® Core™ i7, 8 GB RAM, 256 GB SSD, two network cards Desktop PC (SFF) for OpenLAB® and Chromeleon™ with 24” monitor, German A13111 edition Windows 10 Prof. 64-bit German, Intel® Core™ i7, 8 GB RAM, 256 SSD, two network cards Desktop PC (SFF) for PurityChrom® and ClarityChrom® with 24” monitor, English A13120 edition Windows 10 Prof. 64-bit English, Intel® Core™ i5, 8 GB RAM, 256 GB SSD, two network cards Desktop PC (SFF) for PurityChrom® and ClarityChrom® with 24” monitor, A13110 German edition Windows 10 Prof. 64-bit German, Intel® Core™ i5, 8 GB RAM, 256 SSD, two network cards Laptop for OpenLAB®, Windows 10 Prof. German, Intel® Core™ i5, 8 GB RAM, A13113 500 GB HDD, german edition Laptop for PurityChrom® and ClarityChrom®, Windows 10, min. Intel® Core™ i3, A13112 8 GB RAM, 256 GB SSD, German edition Microsoft Surface Pro for PurityChrom®, Windows 10, Core m3, 4GB RAM, 128 A13114 GB SSD, german edition with keyboard, Surface Pen and docking station Configuration on request Tell us your requirements and we will figure out the matching CDS workstation. A13130 We offer the complete CDS installation and promise you a smooth operation. Network devices WiFi router, 8x LAN GBit RJ-45 ports, 1x WAN GBit RJ-45 port A64809 WiFi router, 8x LAN GBit RJ-45 ports, 1x WAN GBit RJ-45 port, power pl ug UK, US or AUS A64809INT 8-port LAN GBit Switch NetGear GS108GE, 8x RJ-45, GBit, Auto MDI-X A3119 8-port LAN GBit switch NetGear GS108GE, 8x RJ-45, GBit, Auto MDI-X, A3119INT power plug UK, US or AUS 5-port LAN Fast Ethernet Switch NetGear FS105, 5x RJ-45 10/100 MBit A3126 5-port Fast Ethernet switch NetGear FS105 5x RJ-45, 100 MBit, power plug UK, US or AUS A3126INT A64809 16-port LAN Fast Ethernet switch NetGear FS116 16x RJ-45, 10/100 MBit, Auto MDI-X, A3129 power plug UK, US or AUS IT accessories VSCOM USB 4 COM 4 x RS-232 DE9 on USB A3114 AZURA® Interface Box IFU 2.1 LAN, A/D converter, 4 channels AZB00XA RS-232 f/f cable 9-pol nullmodem A0895 RS-232 m/f cable 9-pol A0884 APC Smart UPS 1500 VA, uninterruptible power supply for up to 8 devices A3121 Power cables AZB00XA Power cable for Europe, 2 m, with rubber connector type C13, 230 V M1642 Power cable for Switzerland, 2 m, with rubber connector type C13, 230 V M1597 Power cable for UK, 2.5 m, with rubber connector type C13, 230 V M1278 Power cable for USA, 2 m, with rubber connector type C13, 115 V M1651 Power cable, 1.5 m, with rubber connector for UPS APC Smart connector M2561 Power Supply: Distribution Box 24 V for 6 devices like air sensor, external pressure AZS80SA sensor, IFU 2.1 LAN EU power cable with 4 cold-device plugs and cover caps A12345 AZS80SA 67 | PC Hardware & Periphery PC Hardware & Periphery KNAUER Services With profound application knowledge of analytical and preparative HLPC and FLPC, our team is at your service around the world. Our experts are pleased to receive your inquiries and re- quests and will offer attractive customized solutions. HPLC method development Qualify, quantify or purify Do you plan to separate substances by HPLC in order to qualify, quantify or even purify without spending too much time in developing a suitable method? We offer an application and method development service and supports you to select a suitable system four your lab. According to your specifications we prepare an efficient HPLC or FPLC method including advice for an appropriate sample preparation. HPLC method transfer & optimization For optimized quality and speed Do you intend to perform your analyses faster, more efficient and cost effective? We are happy to support you with its profound expertise and experience in liquid chromatography. The team assists in transferring LC applications and methods. 1. Method transfer We investigate the transfer of your method to one of our HPLC systems. Especially complex separations can cause trouble when transferring them to a different system. We ensure continuous and consistent quality after the transfer. 2. Method optimization Using ultra-pure solvents in HPLC can increase the expenses of an analysis substantially. A shift from classic HPLC columns to smaller inner diameters and smaller particle size could cut costs enormously since consider-ably less solvent is required. We optimize and transfer your LC analyses in order to obtain identical, or even better and faster results, reduce eluent consumption and operating costs. Rent-an-expert Get professional assistance in your lab Some of the numerous chromatographic challenges are better solved in your own lab with your own HPLC equipment. Just order a specialist for your assistance in your lab. In order to develop the best procedure for your HPLC/FPLC or even purification challenge, we will together compile a concept with you in advance. KNAUER Services | 68 Chiral column screening and/or method development and optimization As most chiral separations are not predictable, KNAUER offers a screening service to find the best suiting Eurospher II Chiral column for your chiral separation task. � Column screening with all available Eurospher II Chiral columns � Optional: Method optimization � Results will be reported completely and send as a report � Method parameters and column specifications will be handed over directly Eurokat column screening for the analysis of carbohydrates Not sure which column separates your saccharides best? We offer a screening service for Eurokat columns that are recommended for the separation of sugars and all types of carbohydrates. � Column screening with all available Eurokat col- umns � Optional: Method optimization � Results will be reported completely and send as a report � Method parameters and column specifications will be handed over directly Note: Details and requirements must be discussed previously with KNAUERś application specialists. Find more information about chiral columns at: www.knauer.net/chiralcscreening 69 | KNAUER Services KNAUER has been successfully leading courses for many years for its customers, dealers and sales staff. Our main goal is to familiarize every participant with the latest chromatographic technologies in small groups with practical examples. We offer HPLC courses for beginners and advanced users. In additional courses, participants can receive specialized knowledge, e.g. in UHPLC, FPLC or preparative HPLC. Take part in one of the regularly offered courses or book an individual training on special topics. Workshops at KNAUER in Berlin or on site (see dates online: www.knauer.net/academy) Ordering information upon request: Tel. +49 30 8097270, E-Mail academy@knauer.net HPLC Workshops HPLC Basics (1 day) Practical work in small groups on compact HPLC systems from installation to system performance verification. HPLC Troubleshooting (1 day) Participants gain theoretical as well as practical knowledge in troubleshooting detectors, pumps, autosamplers and columns. HPLC Method Development (1 day) Learn HPLC method development from the beginning and become a HPLC method development pro with our training. FPLC Workshop FPLC Basics & Troubleshooting (1 day) Learn protein purification from the beginning and become a FPLC pro with our training. Prep Workshops Preparative HPLC Basics & Learn preparative LC from the beginning and become a preparative LC pro with our Troubleshooting (1 day) training. SMB Workshops SMB Basics (1 day) The participants will gain basic knowledge on SMB chromatography and be able to perform method development on their own. SMB Method Development (2 days) In this course, SMB method development is explained and practiced using a sample application. Software Workshops ClarityChrom® Software (2 days) Deepen your knowledge and improve your analyses through better software skills. The OpenLAB® EE Software (2 days) courses provide step by step explanation of the software and all components including advanced functions. PurityChrom® Software (1 day) Maintenance Trainings Maintaining KNAUER instruments Learn how to perform preventative maintenance on KNAUER equipment by yourself (individual duration) (at KNAUER in Berlin or on site). Individual Workshops (individual duration, at KNAUER in Berlin or on site) Academy Individual Workshop Do you wish a different course date or a customized workshop for your department? We (individual duration) will gladly help to achieve your individual goals (at KNAUER in Berlin or on site). KNAUER Services | 70 Scientific research generates new results and knowledge for industry and society. Currently, KNAUER is in-volved in different research projects. Obviously, we mostly focus on activities where we can efficiently contrib-ute with our expertise in HPLC technology. With our research commitments, we intend to generate new knowledge in the field of chromatography as well as learn even more about our own products. Are you looking for a competent partner in scientific research projects? Do not hesitate to contact us: academy@knauer.net Qualification Note: Standard procedure for IQ and OQ can be handled differently in individual cases for devices. Installation Qualification (IQ) � certification on the functionality of the device The customer may request the IQ, which is free of charge. In case of a request, the Technical Support of KNAUER or from a provider authorized by KNAUER Instrument IQ Document performs this functionality test during the installation. all instruments VIQ-Installation-Qualification The IQ is a standardized document including: � confirmation of flawless condition at delivery � check if the delivery is complete Instrument / Software OQ Doc. AZURA® Assistant ASM 2.1L, ASM 2.2L VOQ-ASM Operation Qualification (OQ) AZURA® AS 6.1L, AS 3950, PLATINblue AS-1 VOQ-AS The Operation Qualification includes an extensive functionality test according to KNAUER standard OQ AZURA® CM 2.1S VOQCM21SA documents. The Operation Qualification is a standard- AZURA® CT 2.1 column thermostat VOQCT21 ized document and free of charge. It is not part of the delivery, please contact the Technical Support in case AZURA® DAD 6.1L, DAD 2.1L, MWD 2.1L VOQ-DAD of request. AZURA® RID 2.1L, Smartline S2300 VOQ-RID-2.1L The OQ includes the following: AZURA® UVD 2.1L VOQUVD21LA � definition of customer requirements and accep- AZURA® UVD 2.1S VOQUVD21SA tance terms Flow cells VOQ-Flowcells � documentation on device specifications Fraction collectors VOQ-FRC � device functionality check at installation site Osmometer K-7400 VOQ-K7400 Test intervals: To make sure that the device operates within the specified range, you should test the device Osmometer K-7400S VOQ-K7400S regularly. The test intervals are dependent on the Pumps AZURA®, Smartline, usage of the device. VOQ-Pumps BlueShadow, Platinblue Execution: The test can be carried out either by the PurityChrom® VOQ-PUC Technical Support of KNAUER or from a provider RF20A/RF20Axs VOQ-RF20 authorized by KNAUER (for a fee). System OQ for analytical systems VOQ-Sys-01 Valves VOQ-Valves 71 | KNAUER Services Performance Verification (PV) Definition: The document Performance Verification (PV) is part of the quality management system of KNAUER. The Performance Verification includes a reproducibility test of an AZURA Analytical HPLC system and must be purchased from the manufacturer. The PV is a standardized KNAUER document and includes: � Documentation on device specifications � All necessary method parameter to perform the PV Goals: The system runs reliably within the documented specifications and the PV is a summary of the results with comments and evaluations. Target group: The test can be carried out either by the technical support of KNAUER, from a provider authorized by KNAUER or by the customer. System Document AZURA® analytical systems with UV detector used in reversed phase mode VPV-001-AZURA-UV AZURA® analytical systems with RI detector used in reversed phase mode VPV-002-AZURA-RID AZURA® FPLC systems VPV-003-AZURA-FPLC AZURA® analytical systems with FLD detector used in reversed phase mode VPV-004-AZURA-FLD AZURA® SMB Lab and Pilot systems VPV-005-AZURA-SMB AZURA® preparative systems with UV detector used in reversed phase mode VPV-007-AZURA-Prep AZURA® preparative systems with RI detector used in reversed phase mode VPV-008-AZURA-Prep-RID Material certification Upon request customized material certification for all wetted parts with varying degrees of complexity from manufacturer statement (only material) to full documentation (e.g. material certification 3.1, FDA compliance statements). Note: Retrospective material certification is not possible. FAT / SAT The factory acceptance test (FAT) refers to the functional test that is performed by upon completion of the manufacturing process to prove the equip- ment has the same specification and functionality that indicated in the datasheet, specification and purchase order. We are experienced in establishing such test procedures together with you before your equipment is delivered. The acceptance of the equipment at your site (site acceptance test, SAT) is also possible: A technician comes to you and ensures that everything works to your utmost satisfaction. In addition, we can inte- grate the equipment into the existing production environment, if necessary. Capillary labeling Complex HPLC systems with a myriad of valves and variable flow paths can be somewhat confusing. We offer professional capillary labeling upon request, to aid end-users in everyday use. KNAUER Services | 72 We are committed to provide the best quality support with experienced staff and technical expertise. All standard user instructions, helpful video tutorials, and a structured section of frequently asked questions is freely accessible on our web page www.knauer.net. If you need further support, our friendly Support team is happy to help you via e-mail, phone or Team Viewer. They will work with you personally until all issues are resolved. Contact Do you have questions about the installation or the operation of your instrument or software? Support in Germany International Support: (Austria & Switzerland on case-to-case basis): Contact your local KNAUER partner for support: Phone: +49 30 809727-111 (workdays 9-17h CET) www.knauer.net/en/Support/Distributors-worldwide Email: support@knauer.net Our highest goal is to keep your laboratory work as effective and productive as possible. Therefore, we not only pay attention to the highest quality in the development and production of our components and instruments, but also stand by your side after the purchase. With our wide range of services, we are ready to meet any demands to your full satisfaction. KNAUER offers worldwide quality service of all products, purchased from KNAUER or our authorized partners. All KNAUER Service technicians have completed a specialized service training in the KNAUER headquarter in Berlin, Germany. They are ready to help on site ensuring efficient operation and minimized downtime. Installation & Instruction Our experienced KNAUER Service technicans can ensure the proper set-up of your instruments. Get in contact whether you want to use a single device, install a complete system or update your chromatography data system. KNAUER installations always include introduction in proper handling of the devices as well as tips for self-maintenance and imparting of neccessary software knowledge. On request you may add an IQ, OQ, PV or PQ for compliance (see page 71). Maintenance Preventive maintenance has proven to be very successful in ensuring the highest availability of HPLC equipment. Unforeseeable failures of individual system components are thus almost impossible, production processes and laboratory capacities can be planned safely. We offer maintenance services customized to your needs. You may either ship your instruments to the nearest KNAUER Service facility or contact your local dealer for on-site service of an authorized KNAUER Service technican. Repair KNAUER still repairs and maintains the following product lines: the current AZURA®, the former Smartline and PLATINblue devices and - to our best ablilities - the Wellchrom equipment which was introduced in the 90s. If you discover any malfunction of your device don’t worry, we will repair it for you! Please contact you local dealer for shipment matters or ask for an on-site visit of our skilled KNAUER service technicans. 73 | KNAUER Services Software development How does your software limit you? Many devices rely on some kind of software to run and interact with you, either internal software (firmware) or drivers and application software on your PC. Development of firmware for HPLC devices like � UHPLC and HPLC pumps � UV, PDA, RI, detectors � Autosamplers � Valves � Column ovens � Fraction collectors Development of device drivers for � OpenLAB® CDS � ChromeleonTM � HyStar � ClarityChrom® (Clarity based) Automation of measurement and operation process KNAUER software support for firmware, drivers and software solutions To provide the most useful tools for your daily work, our team of software engineers combines its expertise in developing firmware, instrument control drivers, as well as application software. KNAUER also has a long experience in customizing instrument operation and in developing drivers for various OEM customers. Let us know about your software challenges - we will program a solution! Hardware development KNAUER has a long experience in customizing scientific equipment according to your needs. With on-site hardware designers, mechanical production and assembly, we can provide tailor made products under certain conditions. Contact us for more information. At times equipment must be removed from your laboratory or you are forced to order equipment before your laboratory is up and running. We can offer storage facilities where your equipment can be stored for future use, giving you peace of mind knowing that you are protecting your investment. We strongly recommend ordering a KNAUER computer with your HPLC system. However, we understand that sometimes certain constraints do not allow this. We offer a PC configuration service of your PC, in order to assure a safe and reliable installation. Note: We cannot guarantee installation on a non-KNAUER PC. KNAUER Services | 74 Every device is supplied with a power plug of the AZURA® series (cold-device plug) in the suitable country-specific version (see Table 2). Exception of allocation (Table 1) Device Power plug type - BlueShadow Pump 40 P Smartline series (see Table 2) - BlueShadow Detector 40D/50D - Smartline Degasser (article no. A5328) - Osmometer - Router Power plug is supplied. For outside Europe, a suitable adapt- - Switch er is supplied (see Table 2). Degasser (article no. AZE03, AZE03-1, AZE02-1) Power plug is supplied for US, UK, Europe, Australia. - Pressure Control (article no. AZG10) Power distributor (article no. AZS80SA) and accessories kit - Pressure Sensor (article no. AZG10-1) with 1x power plug (article no. F1518) is needed. The distrib- utor can provide power for up to 6 devices. Only one power - Airsensor (article no. A70092, A70093, A70082) distributor per system is required. - Interface Box (article no. AZB00XA) - Power plug for China: Article no. M3027D - Power plug for Australia: Article no. M3027C Tablet for Mobile Control The Tablet includes an european power cable. Order M1279/M1277 in addition for an US/UK power cable. If no suitable adapter is available for a specific country, contact the responsible distributor: www.knauer.net/en/Support/Distributors-worldwide Overview (Table 2) Power plugs/ routers/ Article no. Article no. Article no. Article no. Article no. switches USA UK CH Europe Argentina Power plug AZURA® series M1651 M1278 M1597 M1642 M3233 (cold-device plug) Power plug Smartline series M1279 M1277 M1479-1 M1479 - Router (power plug incl.): A64809INT A64809INT - A64809 - Router WLAN, 8x LAN Adapter: Adapter: M0447V2 M0447V1 Switch (power plug incl.): A3119INT A3119INT - A3119 Switch 8x LAN Adapter: Adapter: M0447V2 M0447V1 Switch (power plug incl.): A3126INT A3126INT - A3126 - Switch 5x LAN Adapter: Adapter: M0447V2 M0447V2 75 | Power Cable Overview Power Cable Overview Note: For connecting multiple devices, we provide a special power plug for up to 4 AZURA® devices (Europe), Article no. A12345. Currently, PCs from KNAUER have no serial interface (RS-232). Thus, to operate the following devices, you must install a serial interface (USB-4COM, Article no. A3114): • Sedex 85 LT • Osmometer (only with software) • Shimadzu RF-20A/Axs, ordered from KNAUER, comes with an RS-232 adapter card PCI-e x1 for desktop computers since this device does not work reliably with USB adapters. Note: If the tablet for Mobile Control should be connected via LAN and not WLAN, the USB-to-LAN Adapter (article no. A96181) is required. You find the driver on the KNAUER website: www.knauer.net/en/usb-lan-adapter AZURA® series (cold-device plug) Smartline series All devices of AZURA® series Analytical Pumps 40P (Article no. APC30xx) PCs and monitors UV Detector 40D/50D Preparative pumps BlueShadow 80P (Article no. APD20xx) Smartline Degasser (Article no. A5328) Micro devices Osmometers - BlueShadow Pump 10P/20P - BlueShadow Detector 10D - Degasser 20DG (Article no. AZE02) Detectors - RF20A (Article no. A59200) - RF20AXS, CBM-20A (Article no. A59201) - Gabi Star - HERM flumo, HERM LB500 - Sedex85LT, Sedex90LT, Sedex100LT, Sedex LC (Article no. A0754-x ) - CHIRALYSER-MP Autosamplers AZURA® Column Thermostat CT 2.1 (Article no. A05852) Fraction collectors - Foxy® R1/R2 (Article no. A59100/A59102/A591021) - LABOCOL Vario-4000 (Article no. A591022/ A591024) External pressure sensor (Article no. AZG10-2) Document no. V1662, version 2.3. Last accessed on 2020/02/17. This document is subject to technical changes. For an up-to-date version, please visit: www.knauer.net/en/cableoverview Power Cable Overview | 76 System Configurators M A K E YOU R PRE SE LEC TION UHPLC HPLC Bio-Inert (SST, max. 1000 bar) (SST, max. 700 bar) (metal-free, max. 400 bar) column 2x/6x A1 A2 B1 B2 detector I detector II pump pump A B ELUENT SELECTION SAMPLE COLUMN SELECTION & DETECTION & DELIVERY INJECTION THERMOSTAT 5 ml/min binary Manual injection 2 column selection UV/VIS DAD 2.1L gradient pump P 6.1L valve single wavelength (UHPLC) 8 column selection DAD 6.1L Autosampler AS 6.1L UV/VIS multiple 5 ml/min quaternary Column thermostat wavelength Fluorescence gradient pump P 6.1L Autosampler AS 6.1L Detector RF-20 A (UHPLC) cool/heat Column kit HPLC Conductivity Fluorescence 10 ml/min binary Column kit UHPLC Refractive index Detector RF-20 Axs gradient pump P 6.1L Eluent pre-heating Light Scattering ECD 2.1 10 ml/min quaternary cartridge pump P 6.1L 0.1 mm ID UHPLC A/D-converter (integration of ..... x solvent selection Eluent pre-heating further detectors) valve (6 further inlets) cartridge 0.18 mm ID HPLC ACCESSORIES 0.1 mm tubing 0.18 mm tubing PEEK tubing ..... x Back pressure Workstation regulator (Windows) FLOW CELLS FOR UV-DETECTOR 10 mm/10 µl 10 mm/2 µl 50 mm/6 µl 3 mm/2 µl (up to 100 ml/min) Pressure proof LightGuide® LightGuide® Pressure proof SOFTWARE ClarityChrom® OpenLAB® Chromeleon™ Mobile Control COMMON APPLICATIONS Reversed phase Normal phase other... System Qualification Analytic HPLC/UHPLC 77 | System Configurators M A K E YOU R PRE SE LEC TION SST Titanium 6x 6x air sensor 5x bypass A1 A2 5x waste B1 B2 detector I detector II fraction collector pump pump sample A B pump 6x BUFFER SELECTION SAMPLE COLUMN SELECTION DETECTION FRACTION & DELIVERY INJECTION & THERMOSTAT COLLECTION 10 ml/min binary Injection valve Column selection UV/VIS Fractionation valve gradient pump P 6.1L (two columns or single wavelength Sample pump module one bypass) Foxy fraction 10 ml/min quaternary UV/VIS multiwave collector with fixed pump P 6.1L Sample selection Column selection length rack types ......valve: ..... x inlets high flow 50 ml/min binary (5 columns, one DAD 2.1L Labocol fraction gradient pump P 6.1L Autosampler AS 6.1L bypass) collector with individual Fluorescence rack types ..... x 100 ml/min pump P 2.1L Autosampler AS 6.1L Detector RF-20 A cool/heat Rack for fraction ..... x 250 ml/min pump P 2.1L Conductivity collector ..... x 500 ml/min pump P 2.1L pH Flow splitter ..... x 1 000 ml/min pump P 2.1L Refractive index Ternary gradient Light Scattering module for pump P 2.1L 4000 MiD Binary gradient module for pump P 2.1L A/D-converter (integration of ..... x solvent selection valve further detectors) (6 further inlets) ACCESSORIES ..... x Airsensor ..... x Airsensor Pressure control ..... x Back pressure AZURA Organizer main pump feed pump (2 pressure sensors) regulator ..... x Tubing 1/16” ..... x Tubing 1/8” ..... x Tubing 1/4” Workstation (Windows) SOFTWARE COMMON APPLICATIONS ClarityChrom® OpenLAB® PurityChrom® Reversed phase Normal phase Chromeleon™ Mobile Control other... System Qualification www.knauer.net/prep Prep HPLC System Configurators | 78 M E THOD SEC AC IEX HIC Size Exclusion Affinity Ion-Exchange Hydrophobic Interaction Chromatography Chromatography Chromatography Chromatography up to 8x up to 8x up to 8x up to 8x air sensor 5x air sensor bypass 5x back A1 A2 pressure up to waste B1 B2 bypass back A1 A2 pressure regulator up to 15x waste B1 B2 regulator 15x UV Con UV Con detector pH fraction collector detector pH fraction collector pump pump pump A pump B sample A B sample pump pump up to 8x up to 8x BUFFER SELECTION SAMPLE COLUMN SELECTION DETECTION FRACTION & DELIVERY INJECTION COLLECTION 10 ml/min binary Multi-Injection valve Column selection UV/VIS Outlet valve gradient pump P 6.1L valve up to 50 ml/min single wavelength ..... x Injection valve (5 columns, one Foxy fraction 10 ml/min quaternary bypass, reverse flow) UV/VIS multiwave- collector with pump P 6.1L Sample pump length fixed rack types .......module Column selection 50 ml/min binary (two columns or Conductivity Labocol fraction gradient pump P 6.1L Sample selection one bypass) collector with ......valve: ..... x inlets pH individual rack types ..... x 100 ml/min pump P 2.1L Column selection Biocompatible high flow Fluorescence Rack for fraction ..... x 250 ml/min pump P 2.1L ......Autosampler AS 6.1L (5 columns, one collector bypass) Refractive index ..... x 500 ml/min pump P 2.1L Column selection Light Scattering ..... x 1 000 ml/min pump P 2.1L high flow (7 columns, one Analog integration Ternary gradient module bypass, reverse flow) of further detectors ......for pump P 2.1L Binary gradient module ......for pump P 2.1L COLU M N S & M E DI A ..... x Buffer selection valve SEC: Desalting ..... ml AC: Protein A ..... ml IEX: DEAE – Weak anion exchange ..... ml ......(8 further inlets) SEC: SEC 75 ..... ml AC: Protein G ..... ml IEX: CM – Weak cation exchange ..... ml SEC: SEC 200 ..... ml AC: Ni-NTA ..... ml IEX: Q – Strong anion exchange ..... ml AC: Glutathione ..... ml IEX: SP – Strong cation exchange ..... ml ACCESSORIES ..... x Air sensor ..... x Air sensor Pressure control ..... x Back pressure AZURA Organizer main pump feed pump (2 pressure sensors) regulator ..... x Tubing 1/16” ..... x Tubing 1/8” ..... x Tubing 1/4” Workstation (Windows) FPLC 79 | System Configurators KNAUER Brochures Please visit www.knauer.net/brochures to find more information about KNAUER products and systems. AZURA® Analytical HPLC/UHPLC AZURA® Bio purification AZURA® Preparative HPLC (Document no. V7852US) (Document no. V7855US) (Document no. V7820US) AZURA® SMB systems Freezing point osmometry KNAUER Column Selection Guide (Document no. V7741US) (Document no. V7716US) (Document no. V7803US) BKNAUER Brochures | 80 Dimensions Tubing volume/length mm inches inches mm Tubing ID μl/cm μl/in 0.10 .004” 1/32” 0.8 .004” 0.08 0.21 0.12 .005” 1/16” 1.6 .005” 0.13 0.32 0.15 .006” 1/8” 3.2 .010” 0.51 1.29 0.25 .010” 1/4” 6.4 .015” 1.14 2.90 0.40 .016” 3/8” 9.5 .020” 2.03 5.15 0.50 .020” 1/2” 12.7 .025” 3.17 8.04 0.75 .030” 1” 25.4 .030” 4.56 11.58 1.00 .040” .040” 8.11 20.59 1.50 .060” .060” 18.24 46.33 2.00 .080” .070” 24.83 63.06 4.60 .180” .085” 36.61 92.99 6.00 .236” 6.40 .253” 7.00 .276” 10.00 .400” Pressure Temperature MPa bar psi °C °F °C °F °C °F 5 50 725 -40 -40 65 149 170 338 10 100 1 450 -35 -31 70 158 175 347 20 200 2 901 -30 -22 75 167 180 356 30 300 4 351 -25 -13 80 176 185 365 40 400 5 802 -20 -4 85 185 190 374 50 500 7 252 -15 5 90 194 195 383 60 600 8 702 -10 14 95 203 200 392 70 700 10 153 -5 23 100 212 205 401 80 800 11 603 0 32 105 221 210 410 90 900 13 054 5 41 110 230 215 419 100 1 000 14 504 10 50 115 239 220 428 110 1 100 15 954 15 59 120 248 225 437 120 1 200 17 405 20 68 125 257 230 446 130 1 300 18 855 25 77 130 266 235 455 140 1 400 20 306 30 86 135 275 240 464 150 1 500 21 756 35 95 140 284 245 473 160 1 600 23 206 40 104 145 293 250 482 170 1 700 24 657 45 113 150 302 255 491 180 1 800 26 107 50 122 155 311 260 500 190 1 900 27 558 55 131 160 320 265 509 200 2 000 29 008 60 140 165 329 270 518 Conversion Tables 81 | 6.2. Claims for damages 1. Definition of terms The liability of KNAUER shall be restricted to intentional acts and The following terms and conditions apply to every order received acts of gross negligence and compensation shall only be due for by KNAUER and every delivery of goods. This holds as well in case direct, foreseeable damages. Liability for breach of a material, of contradictory buying conditions of the purchaser. Exceptions essential duty of the contract, liability because of personal injury, are only valid when confirmed by KNAUER in writing. Purchase liability according to the stipulations of the German Law on orders are only binding if confirmed by KNAUER in writing. Product Liability and liability for the lack of the condition of the contract goods guaranteed by KNAUER remain unaffected. 2. Payment Deliveries are due and payable, net, within 30 days of invoice date 7. Third party rights on industrial or other intellec-or in advance. Deductions are not allowed. Foreign deliveries tual property must be paid by irrevocable letter of credit or in advance. All bank and transfer fees must be paid by the customer. The consequenc-KNAUER shall not be liable for the infringement of third party es arising out of delay are due to statutory provisions. Payments rights founded on industrial or other intellectual property caused are due irrespective of an eventual notice of defect, except such by the use of the delivered goods. The customer is fully responsi-defects are evidently justified. ble for the products manufactured with the goods. In particular KNAUER is not obliged to indemnify and hold harmless the customer from all claims raised by third parties based on the 3. Delivery infringement of their industrial or intellectual property rights by the use of the goods. Delivery dates are not binding unless expressly stated in the contract as binding dates. Delay in delivery requires a written reminder and an adequate additional grace period set by the customer. KNAUER is only liable for claims for damages under the 8. Property rights requirements of no. 6. The ownership of the goods shall remain with KNAUER until payment in full for all our claims resulting from our business relation is received. In case of improper treatment of the goods or 4. Claims in case of default KNAUER may demand the return of the delivered Condition for any warranty claim is the immediate inspection of goods. This demand entails resignation of the contract only if the goods upon delivery, and complaint towards and damage KNAUER declares it explicitly. assessment together with the carrier, and an immediate written complaint to KNAUER. The complaint must be made within five Resellers are allowed to sell the goods to third parties in due workdays in case of visible defects or losses. course of the business. The customer herewith assigns his resale claims against third parties to KNAUER. 5. Risk liability 9. Export Delivery is made at the customer's own risk. As soon as the goods leave KNAUER's plant the risk of accidental loss, destruction or Instruments and products delivered by KNAUER may not be deterioration passes to the customer. exported to a country other than of the customer's headquarters without KNAUER's prior written permission. 6. Warranty and damages 10. Place of settlement and court of jurisdiction 6.1. Warranty claims The place of performance is Berlin. Proper venue for all claims is The warranty begins with receipt of the goods. If commissioning the competent local court at KNAUER's principal place of business has been ordered, after commissioning. In the case of delayed - Berlin. KNAUER reserves the right to sue the customer at his commissioning, the warranty begins at the latest four weeks after principal place of business. receipt of the goods unless the supplier is responsible for delayed commissioning. This agreement shall be governed by the laws of the Federal Republic of Germany excluding the UN-Convention on the The warranty for osmometers and liquid chromatography International Sale of Goods (CISG). instruments is limited to two years, excluding glass breakage, damages due to stoppage and consumable materials such as membranes, light bulbs, columns, bushings, gaskets and valves. KNAUER's liability shall be restricted to the replacement of KNAUER Wissenschaftliche Geräte GmbH defective material or repair only. Transportation costs are borne by the customer. In case of failure of replacement or repair the cus-Hegauer Weg 38 tomer may demand a reduction in price or cancellation of the 14163 Berlin, Germany contract with respect to the defective material. The customer has to inspect the goods delivered immediately and shall immediately give written notification of any defects to KNAUER, in case of non-obvious defects within 10 working days after delivery at the These terms and conditions apply since June 1, 2016 very latest. Terms & Conditions | 82 Terms & Conditions Technical data or prices are subject to change without All trademarks are the property of their respective owners. notice. Prices may vary by country and do not include taxes, Our general terms and conditions apply: customs duties or delivery. www.knauer.net/terms . Distributed by: .1 erap n 2 ly pd ersio S, vU-2 tally frienen7854m 020 V viron en ER 2U on AN © K Printed KNAUER +49 30 809727-0 Wissenschaftliche Geräte GmbH +49 30 8015010 (Fax) Hegauer Weg 38 info@knauer.net 14163 Berlin, Germany www.knauer.net Document Outline Instruments Pumps Assistants Autosamplers Column thermostats Detectors Fraction collectors Degassers Valves Osmometers Accessories Autosampler accessories Osmometry accessories and standards Purification accessories Consumables Lab equipment PC Hardware & Software Mobile Control (Chrom) for Windows 10 ClarityChrom® PurityChrom® OpenLAB® CDS EZChrom Edition Chromeleon™ 7.2 Drivers PC Hardware & Periphery KNAUER Services Chiral Column Screening Services KNAUER Academy Research Compliance Support Worldwide Technical Services Development Services Storage of instruments and systems Configuration of your PC Power Cable Overview System Configurators Prep HPLC FPLC KNAUER Brochures Conversion Tables Terms & Conditions