How do you explain HPLC? 2 AZURA Educational System for tomorrow’s HPLC professionals AZURA Compact HPLC Analytical HPLC for the training of future HPLC professionals We believe that a lively and entertaining training is the best way to inspire school and university students to strive for a career in science. KNAUER is Germany’s largest family-owned manufacturer of HPLC (High Performance Liquid Chromatography) instruments with an experience of over 40 years in this fi eld. Liquid chromatography is one of the most important analytical techniques used in research, envieronmen-tal protection, laboratory diagnostics and quality control today. Let us show you the wide range of HPLC applications and share our knowledge with you. Together with the Hamburg University of Technology, 2 AZURA Compact HPLC: an isocratic HPLC system KNAUER started the KNAUER Explorer Club a couple of with manual injection valve, pump, HPLC column years ago. It aims to give students a solid understan-and UV detector as well as eluent tray and solvent ding of chromatography and its applications such as bottles. the detection and analysis of pollutants in rivers, 2 A laptop and the HPLC software ClarityChrom® ingredients in soft drinks and doping in sports. to control the system and analyze results. 2 Training material: fl ow chart for installation and We would like to support you in your efforts to teach capillary connection, detailed application note and chromatography in theory and practice with the video tutorials. AZURA Educational System. Our special package for training includes: AZURA Compact HPLC: Robust, modern and cost-effective. Discover more with the AZURA Educational System How do you use AZURA Compact HPLC? Intuitive and up-to-date: The clearly defi ned layout helps to understand the different functions of the HPLC system. All important components are easy to access and maintain. The system is controlled by a laptop that also analyzes the measured data. Status LEDs and standby function Pressure transducer with automatic overpressure UV detector with protection records variable wavelength changes in pressure (190 – 500 nm) Venting valve Capillary guides Leak sensor Uses minimal bench space: 52 x 36 cm (L x W) System shown without front cover. Watch the tutorial video online www.knauer.net/cc-tutorials How is the data analyzed? ClarityChrom, the software that controls the HPLC system, is also used to record and interpret the data – for qualitative and quantitative analysis! The included video tutorials show you step-by-step how to use the program and analyze your Robust manual chromatograms. injection valve for sample application What is the AZURA Compact HPLC made up of? Mobile phase Injector Detector Analysis Pump Column Waste High pressure pump delivers eluent (0.01 – 10 ml/min) More information www.knauer.net/azuraedu How do you explain analytical experiments? Your AZURA Educational System includes printed What is an internal training material that explains several applications of HPLC. For example, the quantitative determination standard? of caffeine and paracetamol in samples of your choice (e.g. coffee, soft drinks, medicine) is explained in The internal standard (IS) responds to inaccuracies detail. In simple steps, the students are introduced in quantitative analysis. It is added to the calibration to basic concepts and terms used in HPLC such as solution and samples in a fi xed amount. calibration curve and internal standard. The easy to Ideally, the IS produces the same peak areas in all understand instructions guide them through the measurements. If this should not be the case, the IS can be used to correct for this variability. complete experiment, including the setup, run and analysis. O CH [AU] 3 H 1,4 H C N CH 1 3 N 3 N 1,2 O N O N HO 1,0 CH 0,8 3 ancerb 2 Caffeine Paracetamol so 0,6 Ab 3 0,4 O 0,2 H C 3 NH 0,0 N 0 1 2 3 4 [min.] N Time O N Overlay of chromatograms used for calibration. CH3 1. Paracetamol, 2. Theophylline (IS), 3. Caffeine Theophylline What is included? The AZURA Educational System – Inject, Pump, Separate, Detect and Analyze HPLC system AZURA Compact HPLC with pump P 4.1S and detector UVD 2.1S HPLC system AZURA Educational System, with fl ow cell (10 mm path length, 1/16“ connectors) item no. 671101100 Manual injection valve including 10 μl sample loop Sample application HPLC injection syringe (straight needle), 100 μl volume Injection valve mounting bracket Start-up kit AZURA capillaries and fittings (PEEK, 1/16“) Eluent tray E 2.1L Accessories Set of mobile phase bottles (2 x 1000 ml) Tool kit for HPLC system setup PC Laptop with pre-installed Windows PC communication Router for LAN connection (8 x) ClarityChrom software and licence* for the Software for controlling and recording AZURA Compact HPLC system Reversed-phase column with integrated pre-column, Column kit 125 mm x 4 mm (L x inner diameter), pre-packed with Eurospher II 100-5 C18 Magnetic clip to attach column Easy to understand software tutorial (video on CD) Flow chart for installation Training material Sample applications with detailed information and hand-on experiments (for more isocratic applications, visit www.knauer.net/applications) Need help with installation and setup? Service Contact our Technical Support Team. Phone: +49 30 809727-111, email: support@knauer.net AZURA products and accessories More options (www.knauer.net/azura or request) *license for educational purposes only Technical data are subject to change without notice. Front cover: Painting from street artist El Bocho. Created for the KNAUER Explorer Club (KEK) in which school students learn about the basics of liquid chromatography. www.knauer.net HPLC · SMB · Osmometry KNAUER Phone: +49 30 809727-0 Wissenschaftliche Geräte GmbH Telefax: +49 30 8015010 Hegauer Weg 38 E-Mail: info@knauer.net 14163 Berlin, Germany Internet: www.knauer.net © KNAUER 2016 V7853-1US/2.0/07.16/4T