T E C H N I C A L B U L L E T I N 9 0 9 *Emmes Surveys For research, analysis and control The Autopol® Series of Automatic Polarimeters Exclusive Windows Embedded operating system providing unmatched flexibility The brand of polarimeter used in more labs than any other.* Rudolph Research Analytical 55 Newburgh Road, Hackettstown, NJ 07840 USA • Phone: 973-584-1558 • Fax: 973-584-5440 • E-mail: info@rudolphresearch.com • Internet: www.rudolphresearch.com Polarimetry Polarimetry • Distinguishing between optical isomers. In each of these applications, the AUTOPOL offers up to six discrete A sensitive nondestructive technique for • Analyzing molecular structure by wavelength selections to observe the measuring the optical activity exhibited plotting optical rotatory dispersion effect of wavelength upon an optically by inorganic and organic compounds. curves over a wide range of wavelengths. active substance. A compound is considered to be optically active if linearly polarized light is rotated when passing through it. The amount of optical rotation is determined by the Quality and Process Control molecular structure and concentration of Applications, chiral molecules in the substance. Each both in the laboratory or on-line in optically active substance has its own the factory, are found throughout the specific rotation as defined in Biot’s law: pharmaceutical, essential oil, flavor, food and chemical industries. A few examples PHARMACEUTICALS are listed below. Pharmaceutical Industry Determines product purity by measuring specific rotation and optical rotation of: • Amino acids • Amino sugars • Analgesics • Antibiotics • Cocaine • Codeine • Dextrose • Diuretics • Serums • Steroids The polarimetric method is a simple • Tranquilizers • Vitamins and accurate means for determination and investigation of structure in macro, semi-micro and micro analysis of expensive Flavor, Fragrance and ESSENTIAL OILS and non-duplicable samples. Polarimetry Essential Oil Industry is employed in quality control, process control and research in the pharmaceutical, Utilizes polarimetry for incoming raw chemical, essential oil, flavor and food materials inspection of: industries. It is so well established that • Camphors • Citric acid the United States Pharmacopoeia and • Glyceric acid • Gums the Food & Drug Administration include • Lavender oil • Lemon oil polarimetric specifications for numerous • Orange oil • Spearmint oil substances. Food Industry Research Applications Ensures product quality by measuring the Research applications for polarimetry are concentration and purity of the following found in industry, research institutes and compounds in sugar based foods, cereals universities as a means of: and syrups: SUGAR & CONFECTIONERY PRODUCTS • Isolating and identifying unknowns • Carbohydrates • Fructose crystallized from various solvents, or • Glucose • Lactose separated by high performance liquid • Levulose • Maltose chromatography. • Raffinose • Sucrose • Various starches • Xylose • Evaluating and characterizing optically • Natural monosaccharides active compounds by measuring their specific rotation and comparing this value with the theoretical values found Chemical Industry in literature. • Investigating kinetic reactions by Analyzes optical rotation as a means measuring optical rotation as a function of identifying and characterizing: of time. • Biopolymers • Natural polymers • Monitoring changes in concentration of an • Synthetic polymers optically active component in a reaction mixture, as in enzymatic cleavage. ORGANIC CHEMISTRY Rudolph Research Analytical 55 Newburgh Road, Hackettstown, NJ 07840 USA • Phone: 973-584-1558 • Fax: 973-584-5440 • E-mail: info@rudolphresearch.com • Internet: www.rudolphresearch.com AUTOPOL® Series Unmatched features and flexibility utilizing Windows Embedded CAT 5 Ethernet Rear Panel View Windows Embedded Operating System No virus, no malware and direct Ethernet connection to LIMS or Server without a PC. Connect to almost any USB printer Measurement Modes Cells 10mm to 200mm Long White-light Source Permits The Autopol can read directly in one Instruments of some manufacturers Spectral Versatility of four measurement modes: Optical accept only special sample cells, with The Tungsten-Halogen lamp employed in Rotation, Specific Rotation, Concentration maximum lengths of 100mm. Autopols the Autopol is a compact, reliable, low-cost, or Specific Rotation Plus. Specific Rotation accept standard sample cells up to 200mm high-intensity light source that allows any Plus allows a correction to be applied to a long. A 200mm sample cell offers twice desired wavelength in the visible spectrum result for loss on drying. Each measurement the sensitivity as the same solution in a to be selected by means of a narrow band mode can be activated via touchscreen 100mm cell. This is especially useful for multilayer interference filter. (See Photo 3) selection. Sample cells can be specified at solutions having small rotations. Also The Autopol standard 10nm bandwidth is any length (e.g., 50mm, 100mm, 200mm), some pharmacopeia monographs require a specified to permit high energy throughput or entered as a precise dimension (e.g., 200mm (2dm) cell like the USP monograph and sensitivity for sample transmittances 199.98mm). Concentration is entered as a for Methotrexate. Rudolph Research of only 0.01% (O.D. = 4), while minimizing percentage. (See Photo 1) sample cells are made to NIST standards; the effects of sample ORD and color. and the complete range of sizes and types are listed in Technical Bulletin 913. NIST This lamp is keyed for easy alignment certificates are available for cell length validation. Estimated Life = 2,000 hours Estimated Cost = $100 USD each Photo 1 Temperature Control Photo 3 of Samples Temperature Display Multiple Wavelengths is allowed through rubber gasketing in the The Autopol comes standard with Autopol door which permits tubing to be The Autopol comes in single, dual and a temperature probe which can be connected from an external temperature six wavelength models. Wavelength inserted into the Polarimeter cell as controlled Water Bath to the jacket of selection is completely automatic and is shown below or into the cell probe port. a jacketed polarimeter sample tube. accomplished via menu selection. There (See Photo 4) (See Figure 2) are no lamps or filters to manually remove or insert. The following wavelengths are available: 365nm, 405nm, 436nm, 546nm, 589nm, and 633nm. Optional wavelengths are available; contact the factory for more information. Figure 2 Photo 4 Rudolph Research Analytical 55 Newburgh Road, Hackettstown, NJ 07840 USA • Phone: 973-584-1558 • Fax: 973-584-5440 • E-mail: info@rudolphresearch.com • Internet: www.rudolphresearch.com Choosing a Polarimeter That’s right for your application and budget Autopol ® I Autopol ® III The Autopol I is our entry-level polarimeter model designed for: The Autopol III is Rudolph’s entry level USP pharmaceutical solution: Accuracy: 0.002°C, 0.2% above 1° • University Education • Natural Product Research Standard Wavelengths: 589nm and 546nm • Starch Analysis • Invert Sugar • Lactose in Milk • Vitamins Complete Accessory Package with IQOQPQ documentation, 2 user • Flavors • Fragrance selectable cells and 1 NIST traceable calibration standard with NIST • Chemicals • Limited USP Monograph Certificate Compliance Optional Features: Standard Features: 0.01° Arc Accuracy, 589nm fixed wavelength, • 100mm or 200mm TempTrol™ heating and cooling: built in thermoprobe for temperature measurement, Windows 15°C – 40°C ±.2°C Embedded 7 for direct connection to the network server and flexible • Optional Wavelengths: 405nm, 436nm, 578nm, 633nm, (up to 4 USB printing. wavelengths may be selected or added later. • AP Accuracy Option: ±0.002ůp to 10° Arc,±0.004° 10° - 89°Arc Optional Features: • USR Resolution Option: 0.01, 0.001, 0.0001°, Arc Selectable • 100mm or 200mm TempTrol™ heating and cooling: 15°C – 40°C ±.2°C Below is an example of why Polarimeters with accuracy of ±0.01 are • AP Accuracy Option: 0.004° Arc Optical Rotation not suitable for pharmaceutical applications. Please review the USP is wavelength specific. For USP Pharmaceutical, Rudolph monograph for the material Ofloxacin. Ofloxacin must have a specific recommends 0.002 accuracy for measurements ±1.000 degrees rotation between +1° and -1° at a concentration of 10mg per ml. • USR Resolution Option: 0.01, 0.001°Arc Selectable Biots Law: Autopol ® II [α] = specific rotation, l = optical pathlength in dm; λ = wavelength, T = temperature, α = optical rotation, c = concentration in g/100ml The Autopol I has an accuracy of ±0.02° optical rotation. Below is how its relative accuracy affects a hypothetical Ofloxacin sample: (Specific Rotation) = ±0.01 (Autopol I) (1)(0.01) 10mg/ml (Optical pathlength in dm) .01g/ml 1g/100ml=1%=0.01 Specific Rotation error = ±1.0 You cannot successfully inspect a pharmaceutical (such as Ofloxacin) having a Specific Rotation between+1 and –1 and a 1% concentration, which is quite common for most pharmaceuticals, with an instrument The Autopol II offers the same standard and optional features as the that has an accuracy of ±1.0 in Specific Rotation. Autopol I, but allows greater wavelength flexibility. The Autopol IV has an accuracy of 0.002 for Optical Rotations of 1°Arc Standard Wavelengths: 589nm and 546nm for a total unknown of +/-0.002 under the same conditions: Optional Wavelengths: 365nm, 405nm, 436nm, 578nm, 633nm A total of 2-4 extra wavelengths may be ordered at time of purchase Specific Rotation error = ±0.002 O O or added later when needed. (1)(0.01) F OH Optional Features: Autopol IV SR error = ±0.2 N N • 100mm or 200mm TempTrol™ heating and cooling: N O 15°C – 40°C ±.2°C USP Ofloxacin H C 3 CH3 • AP Accuracy Option: 0.004° Arc Optical Rotation Specific rotation <781S>: between +1° and -1° is wavelength specific Test solution: 10mg per mL, in chloroform • USR Resolution Option: 0.01, 0.001°Arc Selectable Rudolph Research Analytical 55 Newburgh Road, Hackettstown, NJ 07840 USA • Phone: 973-584-1558 • Fax: 973-584-5440 • E-mail: info@rudolphresearch.com • Internet: www.rudolphresearch.com Autopol ® IV Autopol ® V PLUS This model is available in one, two and six wavelength versions and The APV PLUS has all the features of the APV and adds resistance to has a more expensive optical system than the Autopol III. It is the acid erosion with its Silco Steel trough and Hastelloy cell. Furthermore system of choice for research universities, pharmaceutical and fine this instrument comes standard with a 3 rotation quartz standard, chemical research departments. This instrument is also excellent Windows 7 operating system with Windows based navigation, 3 USB for studying racemics and kinetics. Temperature control may be ports to allow quick & easy connection to a mouse, keyboard, printer, accomplished through a circulating Water Bath or with Rudolph’s bar code scanner or memory stick, ethernet port for network cable optional Patented TempTrol™ System, which electronically connection. The Autopol V Plus comes standard with Rudolph’s heats and cools the sample to a specified temperature without Patented TempTrol™ System, which electronically heats and the use of water circulation. cools the sample to a specified temperature without the use of IQOQPQ documentation is included with the instrument. water circulation. The AutoPol V Plus and VI are available with 325nm to measure USP Dextromethorphan Hydrobromide. IQOQPQ documentation is included with the instrument. Autopol ® V Autopol ® VI This is a top of the line six wavelength polarimeter specifically The APVI was developed for the most demanding advanced research designed with the input of the world’s largest pharmaceutical laboratories and features 0.0003 accuracy for low rotation samples of companies. This unit comes standard with 21CFR11 software +/- 1º Arc in addition to all of the features of the APV PLUS including and Rudolph’s Patented TempTrol™ System, which Rudolph’s Patented TempTrol™ System, which electronically electronically heats and cools the sample to a specified heats and cools the sample to a specified temperature without temperature without the use of water circulation. IQOQ the use of water circulation. documentation is included with the instrument. This unit The AutoPol VI is available with 325nm includes all wavelengths necessary to measure 99% of all to measure USP Dextromethorphan Hydrobromide. monographs found in the USP, EP, JP and BP. Please see IQOQPQ documentation is included with the instrument. Technical Bulletin 928 for more details on the Autopol V. IQOQPQ documentation is included with the instrument. Rudolph Research Analytical 55 Newburgh Road, Hackettstown, NJ 07840 USA • Phone: 973-584-1558 • Fax: 973-584-5440 • E-mail: info@rudolphresearch.com • Internet: www.rudolphresearch.com Specifications Models Autopol I Autopol II Autopol III Autopol IV Autopol V Autopol V Plus Autopol VI Optical Rotation, Specific Rotation, Specific Optical Rotation, Specific Rotation, Specific Optical Rotation, Specific Rotation, Specific Optical Rotation, Specific Rotation, Specific Optical Rotation, Specific Rotation, Specific Optical Rotation, Specific Rotation, Specific Optical Rotation, Specific Rotation, Specific Measuring Mode Rotation Plus Concentration, User Defined Scale, Rotation Plus Concentration, User Defined Scale, Rotation Plus Concentration, User Defined Scale, Rotation Plus Concentration, User Defined Rotation Plus Concentration, User Defined Rotation Plus Concentration, User Defined Rotation Plus Concentration, User Defined Sugar Degrees, °Z (ISS) Sugar Degrees, °Z (ISS) Sugar Degrees, °Z (ISS) Scale, Sugar Degrees, °Z (ISS) Scale, Sugar Degrees, °Z (ISS) Scale, Sugar Degrees, °Z (ISS) Scale, Sugar Degrees, °Z (ISS) Measuring Scale Degrees Arc Optical Rotation Degrees Arc Optical Rotation Degrees Arc Optical Rotation Degrees Arc Optical Rotation Degrees Arc Optical Rotation Degrees Arc, % Concentration Degrees Arc, % Concentration 0.01° Arc Optical Rotation, 0.01% 0.01° Arc Optical Rotation, 0.01% 0.001° Arc Optical Rotation, 0.001% 0.001° Arc Optical Rotation, 0.001% 0.001° Arc Optical Rotation, 0.001% 0.001° Arc Optical Rotation, 0.001% 0.0001° Arc Optical Rotation, 0.0001% Resolution Concentration. 0.01 Specific Rotation Concentration, 0.01 Specific Rotation Concentration, 0.001 Specific Rotation Concentration, 0.001 Specific Rotation Concentration, 0.001 Specific Rotation Concentration, 0.001° Specific Rotation Concentration, 0.0001° Specific Rotation 0.01° Arc Optical Rotation, 0.03°Z (ISS) 0.01° Arc Optical Rotation, 0.03°Z (ISS) 0.002° Arc up to 1°, 0.2% above 1°, 0.002ůp to 1°, 0.2% up to 5°, 0.002ůp to 1°, 0.2% up to 5°, 0.002ůp to 1°, 0.2% up to 5°, 0.0003° Arc Optical Rotation Accuracy Sugar Degrees Sugar Degrees 0.01° Z (ISS) 0.01° above 5° 0.01° above 5° 0.01° above 5° See TB 926 Autopol IV Single Accuracy: ±0.002° Arc Optical Rotation Autopol V Plus Single Accuracy (546nm and 589nm): ±0.002° AP Accuracy Option Resolution: 0.001° Arc Optical Rotation Resolution: 0.001° Arc Optical Rotation Accuracy: ±0.002° Arc Reproducibility: 0.002° Arc Optical Rotation, Reproducibility: 0.002° Arc Optical Rotation, Accuracy: ±0.002ůp to 10°, ±0.004° Autopol IV Dual Arc Optical Rotation Autopol V Plus Six Wavelength Accuracy: ±0.004° Arc Optical Rotation Accuracy: ±0.004° Arc Optical Rotation 10° - 89° Arc Optical Rotation Accuracy: ±0.002° Arc Optical Rotation Not applicable Accuracy for other wavelengths is the Accuracy (546nm and 589nm): ±0.002° Arc is wavelength specific is wavelength specific Autopol IV Six Wavelength Accuracy (546nm and 589nm): ±0.002° same as the standard model Accuracy for other wavelengths is the Arc Optical Rotation same as the standard model USR Resolution Option USR Selectable Resolution: 0.01, 0.001° Arc AP Selectable Resolution: 0.01, 0.001° Arc AP Selectable Resolution: 0.01, 0.001, AP Selectable Resolution: 0.01, 0.001, AP Selectable Resolution: 0.01, 0.001, AP Selectable Resolution: 0.01, 0.001, 0.0001° Arc 0.0001° Arc 0.0001° Arc 0.0001° Arc Not applicable Reproducibility 0.01° Arc Optical Rotation 0.01° Arc Optical Rotation 0.002° Arc Optical Rotation 0.002° Arc Optical Rotation 0.002° Arc Optical Rotation 0.002° Arc 0.0002° Arc Optical Rotation Measuring Range ± 89.9° Arc Optical Rotation, ± 999.99° Arc ± 89.9° Arc Optical Rotation, ± 999.99° Arc ± 89.9° Arc Optical Rotation, ± 999.99° Arc ±89.9° Arc Optical Rotation, ±999.99° Arc ±89.9° Arc Optical Rotation, ±999.99° Arc ±89.9° Arc Optical Rotation, ±999.99° Arc ±89.9° Arc Optical Rotation, ±999.99° Arc Specific Rotation, 0-99.9% Concentration Specific Rotation, 0-99.9% Concentration Specific Rotation, 0-99.9% Concentration Specific Rotation, 0-99.9% Concentration Specific Rotation, 0-99.9% Concentration Specific Rotation, 0-99.9% Concentration Specific Rotation, 0-99.9% Concentration Prism Glan Thompson Calcite Glan Thompson Calcite Glan Thompson Calcite Glan Thompson Calcite Glan Thompson Calcite Glan Thompson calcite quartz Glan Thompson calcite quartz 589nm, 546nm standard 589nm, 546nm standard 365nm, 405nm, 436nm, 546nm, 589nm, 365nm, 405nm, 436nm, 546nm, 589nm, 365nm, 405nm, 436nm, 546nm, 589nm, 365nm, 405nm, 436nm, 546nm, 589nm, Optical Wavelengths 589nm Optional wavelengths: 365nm, 405nm, Optional wavelengths: 405nm, 633nm (other wavelengths available: 633nm (other wavelengths available: 633nm (other wavelengths available: 633nm (other wavelengths available: 436nm, 578nm 436nm, 578nm 578nm) 578 nm) 325 nm) 325 nm) Wavelength Selection Fixed Touchscreen Selectable: Touchscreen Selectable: 2 standard, 4 optional 2 standard, 4 optional Touchscreen Selectable:1, 2 and 6 Touchscreen Selectable: 6 standard Touchscreen Selectable: 6 standard Touchscreen Selectable: 6 standard 21 CFR Part 11 Optional: External PC software or Internal Optional: External PC software or Internal Optional: External PC software or Internal Optional: External PC software or Internal Standard: Embedded solution with on/off Compliant Software Embedded solution with on/off option Embedded solution with on/off option Embedded solution with on/off option Optional: External PC software only Optional: External PC software or Internal Embedded solution with on/off option Embedded solution with on/off option option By external water bath (standard) By external water bath (standard) By external water bath (standard) By external water bath (standard) TempTrol™ Automatic Electronic TempTrol™ Automatic Electronic Heating TempTrol™ Automatic Electronic Temperature Control TempTrol™ Automatic Electric Heating TempTrol™ Automatic Electric Heating TempTrol™ Automatic Electric Heating TempTrol™ Automatic Electric Heating and Cooling 15°- 40°C and Cooling 15°- 40°C and Cooling 15°- 40°C and Cooling 15°- 40°C Heating & Cooling 15°-40°C (standard) & Cooling 15°-40°C (standard) Heating & Cooling 15°- 40°C (standard) ±0.2°C (optional) ±0.2°C (optional) ±0.2°C (optional) ±0.2°C (optional) ±0.2°C (standard) ±0.2°C (standard) ±0.2°C (standard) Temp. Probe Range 10°C - 100°C 10°C - 100°C 10°C - 100°C 10°C - 100°C 10°C - 100°C 10°C - 100°C 10°C - 100°C Temp. Probe Accuracy ±0.1°C ±0.1°C ±0.1°C ±0.1°C ±0.1°C ±0.1°C ±0.1°C Measurement Time 5 measurements in less than 25 seconds (avg.) 5 measurements in less than 25 seconds (avg.) 5 measurements in less than 25 seconds (avg.) 4°/sec. slewing rate & 5 sec. nominal 4°/sec. slewing rate & 5 sec. nominal 4°/sec. slew rate and 5 sec. nominal 4°/sec. slew rate and 5 sec. nominal settling time settling time settling time settling time Light Source Standard Tungsten-Halogen 6V, 20W, Tungsten-Halogen 6V, 20W, Tungsten-Halogen 6V, 20W, Tungsten-Halogen 6V, 20W, Tungsten-Halogen 6V, 20W, Tungsten-halogen 6V, 20W, Tungsten-halogen 6V, 20W, avg. 2,000 hour life avg. 2,000 hour life avg. 2,000 hour life avg. 2,000 hour life avg. 2,000 hour life avg. 2,000 hour life avg. 2,000 hour life Light Source Optional LED 20-30 mA 100,00 hr. avg. life N/A N/A N/A N/A N/A N/A Sample Chamber Accepts sample tubes up to 200 mm Accepts sample tubes up to 200 mm Accepts sample tubes up to 200 mm Accepts sample tubes up to 200 mm Accepts sample tubes up to 200 mm Accepts sample tubes up to 200mm Accepts sample tubes up to 200mm Data Storage 32 GB Non-removable Compact Flash 32 GB Non-removable Compact Flash 32 GB Non-removable Compact Flash 32 GB Non-removable Compact Flash 32 GB Non-removable Compact Flash 32 GB Non-removable Compact Flash 32 GB Non-removable Compact Flash 3 – USB Ports, 1-USB 3.0, 2 – RS232 3 – USB Ports, 1-USB 3.0, 2 – RS232 3 – USB Ports, 1-USB 3.0, 2 – RS232 3 – USB Ports, 1-USB 3.0, 2 – RS232 Communication Interface 3 – USB Ports, 1-USB 3.0, 2 – RS232 Ports, 3 – USB Ports, 1-USB 3.0, 2 – RS232 Ports, 3 – USB Ports, 1-USB 3.0, 2 – RS232 Ports, Ethernet Port for Network Connection Ethernet Port for Network Connection Ethernet Port for Network Connection Ports, Ethernet Port for Network Ports, Ethernet Port for Network Ports, Ethernet Port for Network Ports, Ethernet Port for Network Connection Connection Connection Connection Calibration Automatic calibration via touchscreen Automatic calibration via touchscreen Automatic calibration via touchscreen Automatic calibration via touchscreen Automatic calibration via touchscreen Automatic calibration by push-button Automatic calibration via touch screen Operating System Windows Windows Windows Windows Windows Windows Windows Adjustable 10.4 inch diagonal, 800-600 Adjustable 10.4 inch diagonal, 800-600 Display 8” color, 800 x 600 pixel resolution 8” color, 800 x 600 pixel resolution 8” color, 800 x 600 pixel resolution 8” color, 800 x 600 pixel resolution 8” color, 800 x 600 pixel resolution pixels, color, Flat Panel Monitor with pixels, color, Flat Panel Monitor with with 400 nits of brightness with 400 nits of brightness with 400 nits of brightness with 400 nits of brightness with 400 nits of brightness Resistant Touch Screen Interface, Resistant Touch Screen Interface, 200 nits brightness 200 nits brightness User Interface Touchscreen Touchscreen Touchscreen Touchscreen Touchscreen Touchscreen Touchscreen Automatic Sensitivity Measures samples with transmittance Measures samples with transmittance Measures samples with transmittance Measures samples with transmittance Measures samples with transmittance as Measures samples with transmittance Measures samples with transmittance Control as low as 0.01% (up to O.D. 4.0) as low as 0.01% (up to O.D. 4.0) as low as 0.01% (up to O.D. 4.0) as low as 0.01% (up to O.D. 4.0) low as 0.01% (up to O.D. 4.0) as low as 0.01% (up to O.D. 4.0) as low as 0.01% (up to O.D. 4.0) Input Power 100 - 240V, 50/60 Hz 100 - 240V, 50/60 Hz 100 - 240 V, 50/60 Hz 100 - 240 V, 50/60 Hz 100 - 240 V, 50/60 Hz 100 - 240V, 50/60 Hz 100 - 240V, 50/60 Hz Operating Dimensions 24.3” W x 12.7” H x 17.5” D 24.3” W x 12.7” H x 17.5” D 24.3” W x 12.7” H x 17.5” D 30” W x 11.5” H x 17” D 30” W x 11.5” H x 17” D 32” W x 11.5” H x 19.75” D 32” W x 11.5” H x 19.75” D 617 mm W x 323 mm H x 445 mm D 617 mm W x 323 mm H x 445 mm D 617 mm W x 323 mm H x 445 mm D 762 mm W x 431.8 mm H x 279.4 mm D 762 mm W x 431.8 mm H x 279.4 mm D 813 mm W x 292 mm H x 457 mm D 890 mm W x 267 mm H x 432 mm D Operating Weight 42 lbs. (19.05 kg) 42 lbs. (19.05 kg) 42 lbs. (19.05 kg) 85 lbs. (39 kg) 85 lbs. (39 kg) 90 lbs. (41 kg) 90 lbs. (41 kg) Rudolph Research Analytical 55 Newburgh Road, Hackettstown, NJ 07840 USA • Phone: 973-584-1558 • Fax: 973-584-5440 • E-mail: info@rudolphresearch.com • Internet: www.rudolphresearch.com Specifications Models Autopol I Autopol II Autopol III Autopol IV Autopol V Autopol V Plus Autopol VI Optical Rotation, Specific Rotation, Specific Optical Rotation, Specific Rotation, Specific Optical Rotation, Specific Rotation, Specific Optical Rotation, Specific Rotation, Specific Optical Rotation, Specific Rotation, Specific Optical Rotation, Specific Rotation, Specific Optical Rotation, Specific Rotation, Specific Measuring Mode Rotation Plus Concentration, User Defined Scale, Rotation Plus Concentration, User Defined Scale, Rotation Plus Concentration, User Defined Scale, Rotation Plus Concentration, User Defined Rotation Plus Concentration, User Defined Rotation Plus Concentration, User Defined Rotation Plus Concentration, User Defined Sugar Degrees, °Z (ISS) Sugar Degrees, °Z (ISS) Sugar Degrees, °Z (ISS) Scale, Sugar Degrees, °Z (ISS) Scale, Sugar Degrees, °Z (ISS) Scale, Sugar Degrees, °Z (ISS) Scale, Sugar Degrees, °Z (ISS) Measuring Scale Degrees Arc Optical Rotation Degrees Arc Optical Rotation Degrees Arc Optical Rotation Degrees Arc Optical Rotation Degrees Arc Optical Rotation Degrees Arc, % Concentration Degrees Arc, % Concentration 0.01° Arc Optical Rotation, 0.01% 0.01° Arc Optical Rotation, 0.01% 0.001° Arc Optical Rotation, 0.001% 0.001° Arc Optical Rotation, 0.001% 0.001° Arc Optical Rotation, 0.001% 0.001° Arc Optical Rotation, 0.001% 0.0001° Arc Optical Rotation, 0.0001% Resolution Concentration. 0.01 Specific Rotation Concentration, 0.01 Specific Rotation Concentration, 0.001 Specific Rotation Concentration, 0.001 Specific Rotation Concentration, 0.001 Specific Rotation Concentration, 0.001° Specific Rotation Concentration, 0.0001° Specific Rotation 0.01° Arc Optical Rotation, 0.03°Z (ISS) 0.01° Arc Optical Rotation, 0.03°Z (ISS) 0.002° Arc up to 1°, 0.2% above 1°, 0.002ůp to 1°, 0.2% up to 5°, 0.002ůp to 1°, 0.2% up to 5°, 0.002ůp to 1°, 0.2% up to 5°, 0.0003° Arc Optical Rotation Accuracy Sugar Degrees Sugar Degrees 0.01° Z (ISS) 0.01° above 5° 0.01° above 5° 0.01° above 5° See TB 926 Autopol IV Single Accuracy: ±0.002° Arc Optical Rotation Autopol V Plus Single Accuracy (546nm and 589nm): ±0.002° AP Accuracy Option Resolution: 0.001° Arc Optical Rotation Resolution: 0.001° Arc Optical Rotation Accuracy: ±0.002° Arc Reproducibility: 0.002° Arc Optical Rotation, Reproducibility: 0.002° Arc Optical Rotation, Accuracy: ±0.002ůp to 10°, ±0.004° Autopol IV Dual Arc Optical Rotation Autopol V Plus Six Wavelength Accuracy: ±0.004° Arc Optical Rotation Accuracy: ±0.004° Arc Optical Rotation 10° - 89° Arc Optical Rotation Accuracy: ±0.002° Arc Optical Rotation Not applicable Accuracy for other wavelengths is the Accuracy (546nm and 589nm): ±0.002° Arc is wavelength specific is wavelength specific Autopol IV Six Wavelength Accuracy (546nm and 589nm): ±0.002° same as the standard model Accuracy for other wavelengths is the Arc Optical Rotation same as the standard model USR Resolution Option USR Selectable Resolution: 0.01, 0.001° Arc AP Selectable Resolution: 0.01, 0.001° Arc AP Selectable Resolution: 0.01, 0.001, AP Selectable Resolution: 0.01, 0.001, AP Selectable Resolution: 0.01, 0.001, AP Selectable Resolution: 0.01, 0.001, 0.0001° Arc 0.0001° Arc 0.0001° Arc 0.0001° Arc Not applicable Reproducibility 0.01° Arc Optical Rotation 0.01° Arc Optical Rotation 0.002° Arc Optical Rotation 0.002° Arc Optical Rotation 0.002° Arc Optical Rotation 0.002° Arc 0.0002° Arc Optical Rotation Measuring Range ± 89.9° Arc Optical Rotation, ± 999.99° Arc ± 89.9° Arc Optical Rotation, ± 999.99° Arc ± 89.9° Arc Optical Rotation, ± 999.99° Arc ±89.9° Arc Optical Rotation, ±999.99° Arc ±89.9° Arc Optical Rotation, ±999.99° Arc ±89.9° Arc Optical Rotation, ±999.99° Arc ±89.9° Arc Optical Rotation, ±999.99° Arc Specific Rotation, 0-99.9% Concentration Specific Rotation, 0-99.9% Concentration Specific Rotation, 0-99.9% Concentration Specific Rotation, 0-99.9% Concentration Specific Rotation, 0-99.9% Concentration Specific Rotation, 0-99.9% Concentration Specific Rotation, 0-99.9% Concentration Prism Glan Thompson Calcite Glan Thompson Calcite Glan Thompson Calcite Glan Thompson Calcite Glan Thompson Calcite Glan Thompson calcite quartz Glan Thompson calcite quartz 589nm, 546nm standard 589nm, 546nm standard 365nm, 405nm, 436nm, 546nm, 589nm, 365nm, 405nm, 436nm, 546nm, 589nm, 365nm, 405nm, 436nm, 546nm, 589nm, 365nm, 405nm, 436nm, 546nm, 589nm, Optical Wavelengths 589nm Optional wavelengths: 365nm, 405nm, Optional wavelengths: 405nm, 633nm (other wavelengths available: 633nm (other wavelengths available: 633nm (other wavelengths available: 633nm (other wavelengths available: 436nm, 578nm 436nm, 578nm 578nm) 578 nm) 325 nm) 325 nm) Wavelength Selection Fixed Touchscreen Selectable: Touchscreen Selectable: 2 standard, 4 optional 2 standard, 4 optional Touchscreen Selectable:1, 2 and 6 Touchscreen Selectable: 6 standard Touchscreen Selectable: 6 standard Touchscreen Selectable: 6 standard 21 CFR Part 11 Optional: External PC software or Internal Optional: External PC software or Internal Optional: External PC software or Internal Optional: External PC software or Internal Standard: Embedded solution with on/off Compliant Software Embedded solution with on/off option Embedded solution with on/off option Embedded solution with on/off option Optional: External PC software only Optional: External PC software or Internal Embedded solution with on/off option Embedded solution with on/off option option By external water bath (standard) By external water bath (standard) By external water bath (standard) By external water bath (standard) TempTrol™ Automatic Electronic TempTrol™ Automatic Electronic Heating TempTrol™ Automatic Electronic Temperature Control TempTrol™ Automatic Electric Heating TempTrol™ Automatic Electric Heating TempTrol™ Automatic Electric Heating TempTrol™ Automatic Electric Heating and Cooling 15°- 40°C and Cooling 15°- 40°C and Cooling 15°- 40°C and Cooling 15°- 40°C Heating & Cooling 15°-40°C (standard) & Cooling 15°-40°C (standard) Heating & Cooling 15°- 40°C (standard) ±0.2°C (optional) ±0.2°C (optional) ±0.2°C (optional) ±0.2°C (optional) ±0.2°C (standard) ±0.2°C (standard) ±0.2°C (standard) Temp. Probe Range 10°C - 100°C 10°C - 100°C 10°C - 100°C 10°C - 100°C 10°C - 100°C 10°C - 100°C 10°C - 100°C Temp. Probe Accuracy ±0.1°C ±0.1°C ±0.1°C ±0.1°C ±0.1°C ±0.1°C ±0.1°C Measurement Time 5 measurements in less than 25 seconds (avg.) 5 measurements in less than 25 seconds (avg.) 5 measurements in less than 25 seconds (avg.) 4°/sec. slewing rate & 5 sec. nominal 4°/sec. slewing rate & 5 sec. nominal 4°/sec. slew rate and 5 sec. nominal 4°/sec. slew rate and 5 sec. nominal settling time settling time settling time settling time Light Source Standard Tungsten-Halogen 6V, 20W, Tungsten-Halogen 6V, 20W, Tungsten-Halogen 6V, 20W, Tungsten-Halogen 6V, 20W, Tungsten-Halogen 6V, 20W, Tungsten-halogen 6V, 20W, Tungsten-halogen 6V, 20W, avg. 2,000 hour life avg. 2,000 hour life avg. 2,000 hour life avg. 2,000 hour life avg. 2,000 hour life avg. 2,000 hour life avg. 2,000 hour life Light Source Optional LED 20-30 mA 100,00 hr. avg. life N/A N/A N/A N/A N/A N/A Sample Chamber Accepts sample tubes up to 200 mm Accepts sample tubes up to 200 mm Accepts sample tubes up to 200 mm Accepts sample tubes up to 200 mm Accepts sample tubes up to 200 mm Accepts sample tubes up to 200mm Accepts sample tubes up to 200mm Data Storage 32 GB Non-removable Compact Flash 32 GB Non-removable Compact Flash 32 GB Non-removable Compact Flash 32 GB Non-removable Compact Flash 32 GB Non-removable Compact Flash 32 GB Non-removable Compact Flash 32 GB Non-removable Compact Flash 3 – USB Ports, 1-USB 3.0, 2 – RS232 3 – USB Ports, 1-USB 3.0, 2 – RS232 3 – USB Ports, 1-USB 3.0, 2 – RS232 3 – USB Ports, 1-USB 3.0, 2 – RS232 Communication Interface 3 – USB Ports, 1-USB 3.0, 2 – RS232 Ports, 3 – USB Ports, 1-USB 3.0, 2 – RS232 Ports, 3 – USB Ports, 1-USB 3.0, 2 – RS232 Ports, Ethernet Port for Network Connection Ethernet Port for Network Connection Ethernet Port for Network Connection Ports, Ethernet Port for Network Ports, Ethernet Port for Network Ports, Ethernet Port for Network Ports, Ethernet Port for Network Connection Connection Connection Connection Calibration Automatic calibration via touchscreen Automatic calibration via touchscreen Automatic calibration via touchscreen Automatic calibration via touchscreen Automatic calibration via touchscreen Automatic calibration by push-button Automatic calibration via touch screen Operating System Windows Windows Windows Windows Windows Windows Windows Adjustable 10.4 inch diagonal, 800-600 Adjustable 10.4 inch diagonal, 800-600 Display 8” color, 800 x 600 pixel resolution 8” color, 800 x 600 pixel resolution 8” color, 800 x 600 pixel resolution 8” color, 800 x 600 pixel resolution 8” color, 800 x 600 pixel resolution pixels, color, Flat Panel Monitor with pixels, color, Flat Panel Monitor with with 400 nits of brightness with 400 nits of brightness with 400 nits of brightness with 400 nits of brightness with 400 nits of brightness Resistant Touch Screen Interface, Resistant Touch Screen Interface, 200 nits brightness 200 nits brightness User Interface Touchscreen Touchscreen Touchscreen Touchscreen Touchscreen Touchscreen Touchscreen Automatic Sensitivity Measures samples with transmittance Measures samples with transmittance Measures samples with transmittance Measures samples with transmittance Measures samples with transmittance as Measures samples with transmittance Measures samples with transmittance Control as low as 0.01% (up to O.D. 4.0) as low as 0.01% (up to O.D. 4.0) as low as 0.01% (up to O.D. 4.0) as low as 0.01% (up to O.D. 4.0) low as 0.01% (up to O.D. 4.0) as low as 0.01% (up to O.D. 4.0) as low as 0.01% (up to O.D. 4.0) Input Power 100 - 240V, 50/60 Hz 100 - 240V, 50/60 Hz 100 - 240 V, 50/60 Hz 100 - 240 V, 50/60 Hz 100 - 240 V, 50/60 Hz 100 - 240V, 50/60 Hz 100 - 240V, 50/60 Hz Operating Dimensions 24.3” W x 12.7” H x 17.5” D 24.3” W x 12.7” H x 17.5” D 24.3” W x 12.7” H x 17.5” D 30” W x 11.5” H x 17” D 30” W x 11.5” H x 17” D 32” W x 11.5” H x 19.75” D 32” W x 11.5” H x 19.75” D 617 mm W x 323 mm H x 445 mm D 617 mm W x 323 mm H x 445 mm D 617 mm W x 323 mm H x 445 mm D 762 mm W x 431.8 mm H x 279.4 mm D 762 mm W x 431.8 mm H x 279.4 mm D 813 mm W x 292 mm H x 457 mm D 890 mm W x 267 mm H x 432 mm D Operating Weight 42 lbs. (19.05 kg) 42 lbs. (19.05 kg) 42 lbs. (19.05 kg) 85 lbs. (39 kg) 85 lbs. (39 kg) 90 lbs. (41 kg) 90 lbs. (41 kg) Rudolph Research Analytical 55 Newburgh Road, Hackettstown, NJ 07840 USA • Phone: 973-584-1558 • Fax: 973-584-5440 • E-mail: info@rudolphresearch.com • Internet: www.rudolphresearch.com Full cGMP/GLP Compliance Quality cGMP/GLP Printing No matter which model Autopol you choose, Rudolph uses the same Measurement reports can be saved as an Excel or PDF file and edited high quality optics. While other manufacturers use Polaroid Plastic quickly and easily. You can import logos and print your company’s Dichroic Sheet Polarizers, Rudolph does not. Instead, Rudolph uses customized “C of A” directly. the same high quality Glan Thompson Calcite Polarizers in all its models. Why are high quality polarizing prisms important? Because prisms are two of the most critical optical components in the polarimeter. Polaroid polarizers are made of a polymeric plastic where the molecules are stretched and oriented in a specific direction so as to linearly polarize light. These types of plastic sheet polarizers are very Rudolph Research Analytical inexpensive ($50.00 USD) and are vulnerable to heat, warp over time, Tuesday. January 27, 2009 deteriorate from moisture, and also have greater light absorption This sample was measured on an Autopol VI serial number 80179, manufactured by Rudolph Research Analytical,Hackettstown, NJ. than Calcite Polarizers. In many cases, the plastic polarizers must Set Temperature : 20.0 be replaced in 3 – 5 years. Glan Thompson Calcite Polarizers are Temp Corr : OFF comprised of a carbon crystalline structure similar to diamond and n Average Std.Dev. Maximum Minimum have excellent light transmission characteristics. The quality of these 5 34.467 0.0000 34.467 34.467 prisms is so good, Rudolph guarantees its prisms over the life of the S.No Time Result Scale OR °Arc WLG Lg.mm Temp. Comment 1 4:32:05 PM 34.467 OR 34.467 589 100.00 20.0 polarimeter (See Photo 5) 2 4:32:08 PM 34.467 OR 34.467 589 100.00 20.0 3 4:32:10 PM 34.467 OR 34.467 589 100.00 20.0 4 4:32:13 PM 34.467 OR 34.467 589 100.00 20.0 Photo 5 5 4:32:16 PM 34.467 OR 34.467 589 100.00 20.0 Print your customized Certificate of Analysis including your Glan Thompson Thin Film Plastic company logo directly from the Autopol VI® touch screen Calcite Polarizer Sheet Polarizer Rudolph Research Analytical Tuesday, January 27, 2009 This sample was measured on an Autopol VI, serial number 80179, manufactured by Rudolph Research Analytical,Hackettstown, NJ. Set Temperature : 20.0 Temp Corr : OFF n Average Std.Dev. Maximum Minimum 5 34.467 0.0000 34.467 34.467 S.No Time Result Scale OR °Arc WLG Lg.mm Temp. Comment 1 4:32:05 PM 34.467 OR 34.467 589 100.00 20.0 Validation and Calibration 2 4:32:08 PM 34.467 OR 34.467 589 100.00 20.0 3 4:32:10 PM 34.467 OR 34.467 589 100.00 20.0 4 4:32:13 PM 34.467 OR 34.467 589 100.00 20.0 Rudolph Research Analytical knows that you must be able to validate 5 4:32:16 PM 34.467 OR 34.467 589 100.00 20.0 your instrument’s operating performance regularly. Therefore, the Autopol® V, V Plus and VI come standard with the accessories, validation tools and automatic calibration functions necessary to ensure that the temperature control, temperature measurement Capable of making multiple measurements on and optical measurement processes are working accurately and a single sample and reporting complete statistical reproducibly. All functions are accessed simply and conveniently data and all measurement results through the Autopol® liquid sealed touchscreen. NIST Traceable Just listen to our customers Certificate TempTrol™ “We have the AUTOPOL V from Rudolph Research Validation Cell Analytical (RRA)... I have used numerous polarimeters and this is by far the best. We have had it for 1-1/2 years and have had zero problems, the original lamp is still in the instrument. I recommend it over other instruments. RRA installed, trained and performed the IQOQ... It is one piece of equipment that you never worry about.” TempTrol™ Hastelloy™ Measurement Cell Alan Davis – Rockwell Medical Technologies TempTrol™ NIST Traceable Quartz Calibration Standard Rudolph Research Analytical 55 Newburgh Road, Hackettstown, NJ 07840 USA • Phone: 973-584-1558 • Fax: 973-584-5440 • E-mail: info@rudolphresearch.com • Internet: www.rudolphresearch.com RRA13-10 11/17