947 Professional UV/VIS Detector Vario 943 Professional Reactor Vario 943 Professional Thermostat Vario UV/VIS detection – an alternative detection method in ion chromatography UV/VIS Detection – catch the color The 947 Professional UV/VIS Detector Vario enables reli-02 With additional precolumn or postcolumn reaction (PCR) able and accurate quantification of substances that many additional substances can be transformed into absorb light in the ultraviolet or visible range. The instru-UV-active or VIS-active molecules, which makes these ment is operated by the user-friendly MagIC Net soft-substances also detectable with the 947 Professional ware and allows a wide variety of analytes to be deter-UV/VIS Detector Vario. Thus, the range of possible appli-mined by direct or indirect detection. cations is extended even further. Broad area of use 943 Professional Reactor Vario for derivatizations The flow path of Metrohm’s IC instruments is completely For derivatizations of all kinds, the 943 Professional metal-free, as the measuring cell is made of PEEK. Thus, Reactor Vario is the ideal complement to your analytical not only the standard anions and cations, but also transi-system. Its robust design and reaction temperatures of tion metal ions and various organic substances can be up to 150 °C open up a diversity of possible uses for the detected without any problem. Even in complex matri-943 Professional Reactor Vario. ces, e.g. biological samples, good detection limits can be achieved on the basis of the specific absorption proper-The 947 Professional UV/VIS Detector Vario is an alternaties of many analytes. Depending on the application tive to the well-established conductivity detector and there is an option to choose either the single (SW) or the extends the family of intelligent detectors from Metrohm. multi (MW) wavelenght detector for analysis. All Metrohm detectors can be swapped round or combined with each other in a flexible way. 947 Professional UV/VIS Detector Vario extends the range of use of the intelligent IC series. The 947 Professional UV/VIS Detector Vario can be combined with the 940 Professional IC Vario and the 930 Compact IC Flex. 03 Highlights • UV/VIS detector equipped with diode array • Available as Single Wavelength, Multi Wavelength detector • Equipped with longlife lamps • Depending on the application, only the UV lamp or the VIS lamp is necessary • Up to 8 variable wavelengths* • Variable band widths and integration times • Spectrum recording possible at any time* • User-friendly operation thanks to MagIC Net • Many options for data evaluation and representation • Universal use, many combination possibilities • Robust, heatable reactor for precolumn and postcolumn derivatizations • iReactor (intelligent derivatization reactor) • High precision and measuring accuracy • Low detection limits even in complex matrices * This refers to the Multi Wavelenght Detector Very flexible and easy to use The diode array detector opens up the possibility to 04 detect a variety of substances across the entire UV/VIS range. Maximum performance in method development is enabled by flexible settings for data recording. For substances with different absorption maxima there are 8 measuring channels and 1 reference channel available. Furthermore, data can be read out via an analog output. The possibility of connecting a leak sensor makes unat-tended measuring easier. The UV and the VIS lamp are not included in the scope of delivery. This allows you to choose only the necessary lamp for the application at hand. Using the Multiwave Detector, the extensive range of possibilities for data evaluation allows you to obtain, for example, up to 10 absorption maxima of a spectrum automatically. That makes it easier to choose the opti-mum wavelength for a particular application. Complete system for UV/VIS analysis. The minimum setup consists of a 947 Professional UV/VIS Detector Vario – SW, a 943 Professional Thermostat Vario as column compartment and a 942 Extension Module Vario ONE/Deg. Applications Direct extinction Postcolumn derivatization • Nitrogen compounds NO –, NO –, ... • Transition metals 2 3 • Sulfur compounds: S2–, S O 2–, SO 2–, ... • Chromate 2 3 3 • Halogen compounds: IO –, Br–, I–, ... • Bromate 3 • Organic substances: • Silicate - organic acids • Cyanide - vitamins • Ammonium - sweeteners • Aluminum - caffeine, melamine • Amino acids • Other ions: CrO 2–, SCN–, ... • ... 4 Precolumn derivatization • Complexing agents: EDTA, NTA, PBTC, THPC, ... Chromium(VI) in tap water sample Trace level anions and silicate in boiled feed water 05 Chromium(VI) is a carcinogenic element where the con-In the power industry, silicate is a water contaminant that centration in leather, toys, packaging, inks or water should be kept under control to avoid deposits on samples are restricted and need to be monitored. Using boiler or steam turbine blades. Silicate can be determined a post-column derivatisation method with diphenylcar-in low µg/L after derivatisation with a molybdate solu-bazide very low concentrations of chromium(VI) can be tion. Next to the silicate trace anions impurities can be determined. determined in single analytical determination using conductivity detection. 0.40 30 2.0 0.36 27 1.9 0.32 1.8 24 0.28 1.7 U] 21 1.6 0.24 18 1.5 0.20 U] fluoride; 20 µg/L 15 1.4 y [µS/cm] 0.16 chromate; 0.025 mg/L 1.3 Intensity [mA 12 0.12 chloride; 20 µg/L 1.2 9 0.08 Intensity [mA 1.1 Conductivit 6 nitrite; 20 µg/L bromide; 20 µg/L nitrate; 20 µg/L 0.04 0.9 sulfate; 20 µg/L 0.00 3 silicate; 20 µg/L phosphate; 20 µg/L 0.8 0 0.7 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 0 2 4 6 8 10 12 14 16 18 20 22 Time [min] Time [min] Chromate 0.025 µg/L, column: Metrosep A Supp 17 - 100/4.0, Silicate next to standard anions (0.02 mg/L), column: Metrosep eluent: 70 mmol/L NH NO , 7.0 mmol/L NaOH, flow 0.7 mL/min, A Supp 17 - 150/4.0, eluent: 5.0 mmol/L Na CO , 0.2 mmol/L 4 3 2 3 45 °C, postcolumn reagent: 2 mmol/L 1.5 - diphenylcarbazide, NaHCO , flow 0.6 mL/min, 45 °C, postcolumn reagent: 3 10% ethanol, 0.5 mol/L sulphuric acid, flow 0.22 mL/min, 20 mmol/L Na MoO , 200 mmol/L HNO , flow 0.3 mL/min, 2 4 3 sample volume 1.325 mL, wavelength 538 nm. sample volume 200 µL, wavelength 346 nm. Nitrite and nitrate in seawater Bromate in tap water Some ions like nitrite, nitrate, bromide show UV absorp-Bromate is classified as a potential carcinogen and needs tion. These characteristics make it possible to detect them to be monitored. In tap water the maximum contamina-next to high concentration of matrix ions like chloride. In tion level of 10 µg/L (WHO, EC, EPA regulations) should conductivity determination, the chloride peak will overlay not be exceeded. For this application, a specific derivati-the peaks of the mentioned ions and they cannot be sation procedure using Triiodide Method was applied. quantified. Using UV/VIS detection, this problem will be overcome. 16 1.6 14 1.4 1.2 12 1.0 nitrate; 10.0 mg/L 10 U] U] 0.8 8 0.6 6 0.4 bromate; 0.17 µg/L Intensity [mA Intensity [mA 0.2 4 0.0 2 -0.2 0 nitrite; 0.5 mg/L -0.4 bromide; 1.0 mg/L -2 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 0 1 2 3 4 5 6 7 8 9 10 Time [min] Time [min] Nitrite ((0.5 mg/L), bromide (1.0 mg/L) and nitrate (10 mg/L) 0.1 µg/L bromate spiked in tap water sample, column: in artificial sea water, (28 g/L NaCl), column: Metrosep A Supp Metrosep A Supp 16 - 150/4.0, eluent: 100 mmol/L H SO , 2 4 10 - 250/4.0, eluent: 5.0 mmol/L Na CO , 5.0 mmol/L NaHCO , 19.3 µmol/L ammonium heptamolybdate, flow 0.8 mL/min, 2 3 3 flow 1.0 mL/min, 40 °C, chemical suppression, sample volume 45 °C, post-column reagent: 0.27 mmol/L KI, flow 0.15 mL/min 10 µL, wavelength 218 nm. sample volume 1200 µL, wavelength 352 nm. 943 Professional Reactor Vario and 943 Professional Thermostat Vario – the ideal supplement to the 947 Professional UV/VIS Detector Vario 06 The 943 Professional Reactor Vario and the 943 Professional Thermostat Vario are highly flexible. The instrument has metal-free flow paths and can serve as host for other 942 Extension Modules Vario, such as modules with additional pumps or valves. The module can be used both as a heatable precolumn- and postcolumn-reactor and as a column ther mostat. Hence, it is offered in two versions: 943 Professional Reactor Vario: Reactor for 943 Professional Thermostat Vario: Thermostat precolumn or postcolumn reactions for column heating The 943 Professional Reactor Vario features a rapid heat-The heated column oven can be operated with tempera-ing rate and enables precolumn and postcolumn reac-tures as high as 80 °C. Temperature stability is ensured by tions at temperatures as high as 150 °C. Owing to direct a preheating capillary. Up to two columns can be fitted. heat trans fer it is heated up faster and more precisely Monitoring and traceability are also guaranteed in the 943 than conventional reactors or column ovens. Depending Professional Thermostat Vario, as a component of intel i-on the requirement of your application, postcolumn reagent IC. gent and eluent can be brought to the right temperature beforehand or the residence time in the reactor can be flexibly adjusted. Traceability and monitoring are guaranteed in the iReactor by means of a data chip. This integral component of the intelligent IC concept minimizes operator errors and improves the reliability of analysis throughout the analy - tical system. The design of the iReactor enables fast and direct heat transfer. In the column thermostat it is possible to monitor, for example, an iColumn with its precolumn by the column chip. Temperature fluctuations are ruled out by the integral preheating capillary. Technical information 947 Professional UV/VIS Detector Vario 07 Wavelength 190–900 nm (Increment 1) Band width ± 1–50 nm (Increment 1) Integration duration 10–247 ms Measuring duration 50–2'000 ms Measuring principle Diode array with 1024 diodes Lamp lifetime long life UV lamp approx. 2000 h Lamp lifetime long life VIS lamp approx. 5000 h 943 Professional Reactor Vario and 943 Professional Thermostat Vario Temperature stability < 0.05 °C deviation Reproducibility of temperature < ± 0.2 °C Temperature accuracy < 1 °C Heating time < 10 min. from 20 °C to 40 °C Column thermostat Ambient temperature + 5 °C to 80 °C, adjustable in 0.1 °C steps Reactor exchange unit intelligent Ambient temperature + 5 °C to 120 °C, adjustable in 0.1 °C steps Reactor exchange unit high temperature Ambient temperature + 5 °C to 150 °C, adjustable in 0.1 °C steps Without exchange unit Ambient temperature + 5 °C to 150 °C, adjustable in 0.1 °C steps Ordering information 2.947.0010 947 Professional UV/VIS Detector Vario – SW (Single Wavelength) 2.947.0020 947 Professional UV/VIS Detector Vario – MW (Multi Wavelength) 2.943.0110 943 Professional Reactor Vario 2.943.0210 943 Professional Thermostat Vario Accessories for the 947 Professional UV/VIS Detector Vario 6.2804.100 Halogen lamp (VIS) 6.2804.110 Deuterium lamp (UV) Accessories for the 943 Professional Reactor Vario 6.2744.330 Y-connector 3 × UNF 10/32 6.2845.100 Reactor plate complete to Professional Reactor 6.2845.200 Reactor complete for 6.2845.100 6.1015.100 Metrosep BP 1 Guard/2.0 Accessories for the 943 Professional Thermostat Vario 6.1836.020 Preheating capillary with core, 1.44 m 6.2845.600 Column holder complete for 943 Professional Thermostat Vario Peripheral devices and other accessories 6.2061.100 Bottle holder for Professional IC Vario instruments 6.2061.110 Tray with sensor for Professional IC Vario instruments 6.2061.120 System Connector www.metrohm.com G, CH-9100 Herisau ohm A witzerland by Metr , printed in S SW Subject to change Layout by Ecknauer+Schoch A 8.947.5000EN – 2019-08